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The map label for this gene is mdh [H]

Identifier: 213157979

GI number: 213157979

Start: 3586958

End: 3587944

Strand: Reverse

Name: mdh [H]

Synonym: AB57_3474

Alternate gene names: 213157979

Gene position: 3587944-3586958 (Counterclockwise)

Preceding gene: 213157980

Following gene: 213157978

Centisome position: 88.58

GC content: 43.87

Gene sequence:

>987_bases
ATGAAGCAACCCGTTCGTGTTGCCGTGACTGGCGCTGCAGGTCAAATTGGTTACAGCTTATTATTCCGTATCGCAAGCGG
TGAAATGCTAGGTAAAGATCAACCTGTTATTTTACAATTGCTTGAAGTTCCAGTTGAAAAAGCACAACAAGCGCTTAAAG
GCGTAATGATGGAACTTGATGACTGTGCTTTCCCTTTATTGGCTGGCATGATCGGGACTGATGATCCTAAAGTTGCATTT
AAAGATGCTGACTATGCGTTATTGGTAGGTTCTCGTCCACGTGGTCCTGGTATGGAACGTGCTGACTTGTTAAAAGTTAA
CGGCGAAATCTTCATTGGCCAAGGTCAAGCATTAAACGAAGTTGCTAGCCGTGACGTTAAAGTATTAGTTGTAGGTAATC
CTGCAAACACGAATGCTTACATTGCGATGAAATCTGCTCCAGATCTTCCAGCGAAAAACTTCACAGCAATGTTGCGTCTT
GACCACAACCGTGCGTTGACTCAAGTTGCTCAAAAAGCTGGTGTTGCTGTAGCTGACATCGAAAAATTAACAGTTTGGGG
TAACCACTCTCCAACTATGTATGCTGACTACCGTTTTGCAACTGCTAATGGTGAAAGCTTAAAAGACAAGATCAACGATC
CAGCTTGGAACAAAGACGTATTCCTTCCAACTGTTGGTAAACGTGGTGCTGCGATTATCGAAGCGCGTGGTTTGTCTTCT
GCTGCTTCTGCTGCTAACGCTGCAATCGACCATATGCGTGATTGGGCACTTGGTACAAACGGCAAATGGGTAACTATGGG
CGTTCCTTCTGACGGTTCTTATGGTATTCCTGAAGGCGTTATGTTCGGTTTCCCTGTAACAACTGAAAACGGTGAATACA
AAATCGTTCAAGGTTTAGAAATCGATGAATTCAGCCGTGAGCGTATCAACTTCACTCTAAACGAGCTTGAAGAAGAACGT
GCAGCAATTGCTGACATGGTAAAATAA

Upstream 100 bases:

>100_bases
TCTGCCACTTCTTTAGAGTACAATCTTTGCCGTTTATGATGATTTGGTTAAGTAGAATCGCTACTTAACCCGATTAAGGA
CACTCATTGGAGACAATTAC

Downstream 100 bases:

>100_bases
TTCATCAGTTTGATGAGTTGGAAAGCACTCTCTTGTAGGGTGCTTTTTTTATATGTGAAATTTAATAAGAACACACAAAA
AACAACAAGAAAATATAGGC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKQPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLEVPVEKAQQALKGVMMELDDCAFPLLAGMIGTDDPKVAF
KDADYALLVGSRPRGPGMERADLLKVNGEIFIGQGQALNEVASRDVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLRL
DHNRALTQVAQKAGVAVADIEKLTVWGNHSPTMYADYRFATANGESLKDKINDPAWNKDVFLPTVGKRGAAIIEARGLSS
AASAANAAIDHMRDWALGTNGKWVTMGVPSDGSYGIPEGVMFGFPVTTENGEYKIVQGLEIDEFSRERINFTLNELEEER
AAIADMVK

Sequences:

>Translated_328_residues
MKQPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLEVPVEKAQQALKGVMMELDDCAFPLLAGMIGTDDPKVAF
KDADYALLVGSRPRGPGMERADLLKVNGEIFIGQGQALNEVASRDVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLRL
DHNRALTQVAQKAGVAVADIEKLTVWGNHSPTMYADYRFATANGESLKDKINDPAWNKDVFLPTVGKRGAAIIEARGLSS
AASAANAAIDHMRDWALGTNGKWVTMGVPSDGSYGIPEGVMFGFPVTTENGEYKIVQGLEIDEFSRERINFTLNELEEER
AAIADMVK
>Mature_328_residues
MKQPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLEVPVEKAQQALKGVMMELDDCAFPLLAGMIGTDDPKVAF
KDADYALLVGSRPRGPGMERADLLKVNGEIFIGQGQALNEVASRDVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLRL
DHNRALTQVAQKAGVAVADIEKLTVWGNHSPTMYADYRFATANGESLKDKINDPAWNKDVFLPTVGKRGAAIIEARGLSS
AASAANAAIDHMRDWALGTNGKWVTMGVPSDGSYGIPEGVMFGFPVTTENGEYKIVQGLEIDEFSRERINFTLNELEEER
AAIADMVK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family [H]

Homologues:

Organism=Homo sapiens, GI5174539, Length=328, Percent_Identity=51.8292682926829, Blast_Score=334, Evalue=7e-92,
Organism=Homo sapiens, GI89886456, Length=345, Percent_Identity=24.6376811594203, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17561064, Length=326, Percent_Identity=54.2944785276074, Blast_Score=328, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI32566798, Length=264, Percent_Identity=52.6515151515151, Blast_Score=250, Evalue=6e-67,
Organism=Caenorhabditis elegans, GI32566800, Length=169, Percent_Identity=52.0710059171598, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24583394, Length=325, Percent_Identity=53.8461538461538, Blast_Score=326, Evalue=1e-89,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 35324; Mature: 35324

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLEVPVEKAQQALKGVMMELD
CCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
DCAFPLLAGMIGTDDPKVAFKDADYALLVGSRPRGPGMERADLLKVNGEIFIGQGQALNE
HHHHHHHHHHCCCCCCCEEEECCCEEEEECCCCCCCCCCCCEEEEECCEEEEECCCHHHH
VASRDVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLRLDHNRALTQVAQKAGVAVADI
HHCCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCEEEEE
EKLTVWGNHSPTMYADYRFATANGESLKDKINDPAWNKDVFLPTVGKRGAAIIEARGLSS
EEEEEECCCCCCEEEEEEEEECCCCHHHHHCCCCCCCCCEEEECCCCCCCEEEEECCCCH
AASAANAAIDHMRDWALGTNGKWVTMGVPSDGSYGIPEGVMFGFPVTTENGEYKIVQGLE
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEECEEECCCCCEEEEECCC
IDEFSRERINFTLNELEEERAAIADMVK
CCHHCHHHHCEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLEVPVEKAQQALKGVMMELD
CCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
DCAFPLLAGMIGTDDPKVAFKDADYALLVGSRPRGPGMERADLLKVNGEIFIGQGQALNE
HHHHHHHHHHCCCCCCCEEEECCCEEEEECCCCCCCCCCCCEEEEECCEEEEECCCHHHH
VASRDVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLRLDHNRALTQVAQKAGVAVADI
HHCCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCEEEEE
EKLTVWGNHSPTMYADYRFATANGESLKDKINDPAWNKDVFLPTVGKRGAAIIEARGLSS
EEEEEECCCCCCEEEEEEEEECCCCHHHHHCCCCCCCCCEEEECCCCCCCEEEEECCCCH
AASAANAAIDHMRDWALGTNGKWVTMGVPSDGSYGIPEGVMFGFPVTTENGEYKIVQGLE
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEECEEECCCCCEEEEECCC
IDEFSRERINFTLNELEEERAAIADMVK
CCHHCHHHHCEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA