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The map label for this gene is yfiN [H]

Identifier: 213157945

GI number: 213157945

Start: 3550843

End: 3551943

Strand: Direct

Name: yfiN [H]

Synonym: AB57_3439

Alternate gene names: 213157945

Gene position: 3550843-3551943 (Clockwise)

Preceding gene: 213157944

Following gene: 213157946

Centisome position: 87.66

GC content: 33.88

Gene sequence:

>1101_bases
ATGGAAACTTATGCAAAACAAAATTTGCAATTACTTAGCCATACTCTTCTTGAGCGTATTCAGCCTGCCGTTGTGTTTAA
CGATAAGATCACGATCGAACAAATCTTAAATGAATACACAAATGATCACTCTATTCGAGCGATTCATATTTATGACTCTG
AACATCACCTGATTGCTCAAAGTTTTAAGTTGTCGAGTCATACTTCAGTGTTAGAGAAATGGTTTGATCACTGGTTTTTA
AATGAGCCGGTACATTTAACGATTTATCATCATCAACAGAATGTAGGTGAACTCACCCTTTTTGGTAGCTCTGAAAAAAT
ATTGCAATTTTTAAAGATGATTATGGTCGGCCTTGCTATTGCGATGCTATTTATTGTTTGTGCATTGTGGTGGTCAGTGA
ATTTGACTTATCGTCAAATTATGCAAGCGATTTATCCACTCACAAATATTGCTCAAATCGTGAGTGAACAAAAAGCTTAT
AACCTTCGTTTTCCTTATAACCGAATTAAAGAGTTTCAAGATTTAAATACAGTTTTTAATGAGTTACTTGAAGAAATTCA
GATTTGGGATAATCAGCTGCAAAAGGAAAATCGTAAATTATCGTTTCAAGCTCATCATGACCAGCTGACCCTACTACCGA
ATAGACATTATTTTTATCAAATACTATTAGATATGTTTGAAGATAAGGATTTTGATAATAAGTCAGCCTTATTGTTTATA
GATAACAATAACTTCAAATCAATTAATGATCAGTTCGGACATTTAGCTGGTGATGAGGTTTTAAAAGAGATGGCGAAAAG
GTTACAAACGCGTTTACGTCATCAAGATTTTATTGCCCGTTTAGGCGGTGATGAATTTGCGGTTATTTTGCATTCCATTA
CGCATGCCGATCATTTAATATCTATTGCTGAAAATTTACTCGAAAGCTGTAAAGAACCTTTGCATCTAAATGATCAAACG
ATTTATTTCAGTTTTAGTGTAGGGATTGCTTTATCCCAATTTGCGTCTAGTCCAGAAGATTTTATTATGCAAGCAGATCA
AGCGATGTATAAGGCTAAGACTTCTGAGCAACATTGGTTTATTTATAAACCAGAGAATTAA

Upstream 100 bases:

>100_bases
TCCACTTCGCTACATGCTACTTTAGAAGTCTCAATTTACGATCTTTGCTATAACTTTCTTTATTTGCTCTTTTACATTTG
TATCAATTTCCGTTTTTACG

Downstream 100 bases:

>100_bases
ATTTAGGCTAATTTATGAACACAAGATCTTTGAAACTCTCCTTTATTGCATTGCTGTGCATTGCGCTTGCAGGCTGCTTG
AGTTTTGGTCCTTTGAAATA

Product: GGDEF domain protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
METYAKQNLQLLSHTLLERIQPAVVFNDKITIEQILNEYTNDHSIRAIHIYDSEHHLIAQSFKLSSHTSVLEKWFDHWFL
NEPVHLTIYHHQQNVGELTLFGSSEKILQFLKMIMVGLAIAMLFIVCALWWSVNLTYRQIMQAIYPLTNIAQIVSEQKAY
NLRFPYNRIKEFQDLNTVFNELLEEIQIWDNQLQKENRKLSFQAHHDQLTLLPNRHYFYQILLDMFEDKDFDNKSALLFI
DNNNFKSINDQFGHLAGDEVLKEMAKRLQTRLRHQDFIARLGGDEFAVILHSITHADHLISIAENLLESCKEPLHLNDQT
IYFSFSVGIALSQFASSPEDFIMQADQAMYKAKTSEQHWFIYKPEN

Sequences:

>Translated_366_residues
METYAKQNLQLLSHTLLERIQPAVVFNDKITIEQILNEYTNDHSIRAIHIYDSEHHLIAQSFKLSSHTSVLEKWFDHWFL
NEPVHLTIYHHQQNVGELTLFGSSEKILQFLKMIMVGLAIAMLFIVCALWWSVNLTYRQIMQAIYPLTNIAQIVSEQKAY
NLRFPYNRIKEFQDLNTVFNELLEEIQIWDNQLQKENRKLSFQAHHDQLTLLPNRHYFYQILLDMFEDKDFDNKSALLFI
DNNNFKSINDQFGHLAGDEVLKEMAKRLQTRLRHQDFIARLGGDEFAVILHSITHADHLISIAENLLESCKEPLHLNDQT
IYFSFSVGIALSQFASSPEDFIMQADQAMYKAKTSEQHWFIYKPEN
>Mature_366_residues
METYAKQNLQLLSHTLLERIQPAVVFNDKITIEQILNEYTNDHSIRAIHIYDSEHHLIAQSFKLSSHTSVLEKWFDHWFL
NEPVHLTIYHHQQNVGELTLFGSSEKILQFLKMIMVGLAIAMLFIVCALWWSVNLTYRQIMQAIYPLTNIAQIVSEQKAY
NLRFPYNRIKEFQDLNTVFNELLEEIQIWDNQLQKENRKLSFQAHHDQLTLLPNRHYFYQILLDMFEDKDFDNKSALLFI
DNNNFKSINDQFGHLAGDEVLKEMAKRLQTRLRHQDFIARLGGDEFAVILHSITHADHLISIAENLLESCKEPLHLNDQT
IYFSFSVGIALSQFASSPEDFIMQADQAMYKAKTSEQHWFIYKPEN

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1788956, Length=360, Percent_Identity=30.5555555555556, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1787541, Length=174, Percent_Identity=35.632183908046, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786584, Length=170, Percent_Identity=35.2941176470588, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87081977, Length=177, Percent_Identity=35.5932203389831, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87081881, Length=236, Percent_Identity=29.2372881355932, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788381, Length=98, Percent_Identity=43.8775510204082, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787802, Length=312, Percent_Identity=23.3974358974359, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787056, Length=152, Percent_Identity=36.1842105263158, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=29.8136645962733, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87082007, Length=166, Percent_Identity=34.3373493975904, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI145693134, Length=151, Percent_Identity=33.112582781457, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788085, Length=158, Percent_Identity=31.6455696202532, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 43076; Mature: 43076

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METYAKQNLQLLSHTLLERIQPAVVFNDKITIEQILNEYTNDHSIRAIHIYDSEHHLIAQ
CCCHHHHHHHHHHHHHHHHCCCCEEECCCEEHHHHHHHHCCCCCEEEEEEECCCCHHHHH
SFKLSSHTSVLEKWFDHWFLNEPVHLTIYHHQQNVGELTLFGSSEKILQFLKMIMVGLAI
HHCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHH
AMLFIVCALWWSVNLTYRQIMQAIYPLTNIAQIVSEQKAYNLRFPYNRIKEFQDLNTVFN
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCHHHHHHHHHHHHHHH
ELLEEIQIWDNQLQKENRKLSFQAHHDQLTLLPNRHYFYQILLDMFEDKDFDNKSALLFI
HHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEE
DNNNFKSINDQFGHLAGDEVLKEMAKRLQTRLRHQDFIARLGGDEFAVILHSITHADHLI
ECCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SIAENLLESCKEPLHLNDQTIYFSFSVGIALSQFASSPEDFIMQADQAMYKAKTSEQHWF
HHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEE
IYKPEN
EECCCC
>Mature Secondary Structure
METYAKQNLQLLSHTLLERIQPAVVFNDKITIEQILNEYTNDHSIRAIHIYDSEHHLIAQ
CCCHHHHHHHHHHHHHHHHCCCCEEECCCEEHHHHHHHHCCCCCEEEEEEECCCCHHHHH
SFKLSSHTSVLEKWFDHWFLNEPVHLTIYHHQQNVGELTLFGSSEKILQFLKMIMVGLAI
HHCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHH
AMLFIVCALWWSVNLTYRQIMQAIYPLTNIAQIVSEQKAYNLRFPYNRIKEFQDLNTVFN
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCHHHHHHHHHHHHHHH
ELLEEIQIWDNQLQKENRKLSFQAHHDQLTLLPNRHYFYQILLDMFEDKDFDNKSALLFI
HHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEE
DNNNFKSINDQFGHLAGDEVLKEMAKRLQTRLRHQDFIARLGGDEFAVILHSITHADHLI
ECCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SIAENLLESCKEPLHLNDQTIYFSFSVGIALSQFASSPEDFIMQADQAMYKAKTSEQHWF
HHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEE
IYKPEN
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 6357787; 7567469 [H]