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The map label for this gene is tgt [H]

Identifier: 213157923

GI number: 213157923

Start: 3525479

End: 3526612

Strand: Direct

Name: tgt [H]

Synonym: AB57_3415

Alternate gene names: 213157923

Gene position: 3525479-3526612 (Clockwise)

Preceding gene: 213157922

Following gene: 213157924

Centisome position: 87.04

GC content: 42.06

Gene sequence:

>1134_bases
ATGAAGTTTGAAAAATTAGGTCAGTCGGGTCGTGCCCGTCGTGGTCGTTTAACTTTAGAACATGGTGTTGTTGAAACCCC
TGTTTTTATGCCGGTGGGGACTTACGGTACAGTCAAAGGCATGCTGCCACGTGATATTGAAGATATTCAGGCACAAATTA
TTTTAGGTAATACCTTCCATTTATATTTGCGTCCGGGCCTTGAAGTGATCAAGCAACACGGCGGCTTGCACGACTTTATT
AAATGGAATAAGCCAATTTTGACCGACTCTGGCGGCTTCCAAGTATTTAGTCTTGGCGCTATGCGTAAAATTAAGGAAGA
AGGTGTTACTTTCCGTTCTCCGATTGATGGTTCAAAAGTCTTTTTATCGCCTGAAATTTCAATGGAAATTCAGCATGTAT
TGAACTCTGACATTGTCATGATTTTTGATGAATGTACGCCTTATCCTGCGACACATGAAGAAGCACAAAAATCATTACAG
CTTTCTTTGCGCTGGGCAAAACGCTGTAAGGCTCATCACCACGATGAACTTAAAAATAAAAATGCGTTATTTGGCATTAT
TCAAGGCGGCATGTATGAAGACTTACGTGATGAGTCTTTAAATGGCTTACTTGAAATCGGCTTTGACGGTTACGCAATTG
GCGGTTTGTCAGTTGGTGAACCAAAAGAAGAGATGATCAAGGTTTTAGATTATCTTCCAAACAAGATGCCGCATGACAAA
CCACGTTACCTGATGGGTGTAGGTAAACCTGAAGATATCGTTGAAGCGGTACGCCGTGGTGTCGATATGTTTGACTGTGT
AATGCCAACACGTAATGCACGTAATGGTCATTATTTTGTCACTGATGGTCTGGTACGTATACGTAATAGTAAATATCGTC
ATGACCAAGGACCACTTGACCCACACTGCGATTGCTATACCTGTAAGAACTTTACTCGTGCTTACCTGTTCCACCTTGAG
AAGTGTGGAGAGATGCTGGCGTCTATGCTTGGTACCATTCATAACTTACGTTATTACCAACGTTTAACTGAAGGTATGCG
TGATGCATTAGACAATGGCACTTTTGATGAATTTGTTCAGGACTTTTATGCCCGCCGCGGATTAGAAGTTCCACCATGCC
CTGTCGATGAATAA

Upstream 100 bases:

>100_bases
GTCGCAGAATCAAGGAAAATAGCCCTCTTTTTAGCAGCGAACTGTTTTTTCGCCCTTGCACTGCCCTACGGCATTGCTTG
CTATTGTTCAGGATTTTTAA

Downstream 100 bases:

>100_bases
GCGAGATTTGCGCTCAACCTCAAGACAAGGTAAATTGCAAAACGAATTAGGATTATGTCAGGTATCTGACTCATATTTTT
TAACTTTAGGAAATTAAAAT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MKFEKLGQSGRARRGRLTLEHGVVETPVFMPVGTYGTVKGMLPRDIEDIQAQIILGNTFHLYLRPGLEVIKQHGGLHDFI
KWNKPILTDSGGFQVFSLGAMRKIKEEGVTFRSPIDGSKVFLSPEISMEIQHVLNSDIVMIFDECTPYPATHEEAQKSLQ
LSLRWAKRCKAHHHDELKNKNALFGIIQGGMYEDLRDESLNGLLEIGFDGYAIGGLSVGEPKEEMIKVLDYLPNKMPHDK
PRYLMGVGKPEDIVEAVRRGVDMFDCVMPTRNARNGHYFVTDGLVRIRNSKYRHDQGPLDPHCDCYTCKNFTRAYLFHLE
KCGEMLASMLGTIHNLRYYQRLTEGMRDALDNGTFDEFVQDFYARRGLEVPPCPVDE

Sequences:

>Translated_377_residues
MKFEKLGQSGRARRGRLTLEHGVVETPVFMPVGTYGTVKGMLPRDIEDIQAQIILGNTFHLYLRPGLEVIKQHGGLHDFI
KWNKPILTDSGGFQVFSLGAMRKIKEEGVTFRSPIDGSKVFLSPEISMEIQHVLNSDIVMIFDECTPYPATHEEAQKSLQ
LSLRWAKRCKAHHHDELKNKNALFGIIQGGMYEDLRDESLNGLLEIGFDGYAIGGLSVGEPKEEMIKVLDYLPNKMPHDK
PRYLMGVGKPEDIVEAVRRGVDMFDCVMPTRNARNGHYFVTDGLVRIRNSKYRHDQGPLDPHCDCYTCKNFTRAYLFHLE
KCGEMLASMLGTIHNLRYYQRLTEGMRDALDNGTFDEFVQDFYARRGLEVPPCPVDE
>Mature_377_residues
MKFEKLGQSGRARRGRLTLEHGVVETPVFMPVGTYGTVKGMLPRDIEDIQAQIILGNTFHLYLRPGLEVIKQHGGLHDFI
KWNKPILTDSGGFQVFSLGAMRKIKEEGVTFRSPIDGSKVFLSPEISMEIQHVLNSDIVMIFDECTPYPATHEEAQKSLQ
LSLRWAKRCKAHHHDELKNKNALFGIIQGGMYEDLRDESLNGLLEIGFDGYAIGGLSVGEPKEEMIKVLDYLPNKMPHDK
PRYLMGVGKPEDIVEAVRRGVDMFDCVMPTRNARNGHYFVTDGLVRIRNSKYRHDQGPLDPHCDCYTCKNFTRAYLFHLE
KCGEMLASMLGTIHNLRYYQRLTEGMRDALDNGTFDEFVQDFYARRGLEVPPCPVDE

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=365, Percent_Identity=43.5616438356164, Blast_Score=311, Evalue=6e-85,
Organism=Escherichia coli, GI1786607, Length=376, Percent_Identity=67.8191489361702, Blast_Score=545, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI17544680, Length=363, Percent_Identity=41.8732782369146, Blast_Score=291, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI25146502, Length=157, Percent_Identity=31.8471337579618, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19920510, Length=367, Percent_Identity=45.2316076294278, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI21355311, Length=258, Percent_Identity=25.5813953488372, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42913; Mature: 42913

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFEKLGQSGRARRGRLTLEHGVVETPVFMPVGTYGTVKGMLPRDIEDIQAQIILGNTFH
CCHHHHCCCCCCCCCCEEECCCCCCCCCEEECCCCCCCCCCCCCCHHHHEEEEEECCEEE
LYLRPGLEVIKQHGGLHDFIKWNKPILTDSGGFQVFSLGAMRKIKEEGVTFRSPIDGSKV
EEECCHHHHHHHCCCHHHHHHCCCCEEECCCCCEEEEHHHHHHHHHCCCEEECCCCCCEE
FLSPEISMEIQHVLNSDIVMIFDECTPYPATHEEAQKSLQLSLRWAKRCKAHHHDELKNK
EECCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NALFGIIQGGMYEDLRDESLNGLLEIGFDGYAIGGLSVGEPKEEMIKVLDYLPNKMPHDK
CCEEEEECCCCHHHHHHHHCCCEEEECCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCC
PRYLMGVGKPEDIVEAVRRGVDMFDCVMPTRNARNGHYFVTDGLVRIRNSKYRHDQGPLD
CCEEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEECCCCEECCCCCCCCCCCCC
PHCDCYTCKNFTRAYLFHLEKCGEMLASMLGTIHNLRYYQRLTEGMRDALDNGTFDEFVQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
DFYARRGLEVPPCPVDE
HHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKFEKLGQSGRARRGRLTLEHGVVETPVFMPVGTYGTVKGMLPRDIEDIQAQIILGNTFH
CCHHHHCCCCCCCCCCEEECCCCCCCCCEEECCCCCCCCCCCCCCHHHHEEEEEECCEEE
LYLRPGLEVIKQHGGLHDFIKWNKPILTDSGGFQVFSLGAMRKIKEEGVTFRSPIDGSKV
EEECCHHHHHHHCCCHHHHHHCCCCEEECCCCCEEEEHHHHHHHHHCCCEEECCCCCCEE
FLSPEISMEIQHVLNSDIVMIFDECTPYPATHEEAQKSLQLSLRWAKRCKAHHHDELKNK
EECCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NALFGIIQGGMYEDLRDESLNGLLEIGFDGYAIGGLSVGEPKEEMIKVLDYLPNKMPHDK
CCEEEEECCCCHHHHHHHHCCCEEEECCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCC
PRYLMGVGKPEDIVEAVRRGVDMFDCVMPTRNARNGHYFVTDGLVRIRNSKYRHDQGPLD
CCEEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEECCCCEECCCCCCCCCCCCC
PHCDCYTCKNFTRAYLFHLEKCGEMLASMLGTIHNLRYYQRLTEGMRDALDNGTFDEFVQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
DFYARRGLEVPPCPVDE
HHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA