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The map label for this gene is btuB [H]

Identifier: 213157889

GI number: 213157889

Start: 3485567

End: 3487441

Strand: Direct

Name: btuB [H]

Synonym: AB57_3381

Alternate gene names: 213157889

Gene position: 3485567-3487441 (Clockwise)

Preceding gene: 213157879

Following gene: 213157890

Centisome position: 86.05

GC content: 37.49

Gene sequence:

>1875_bases
ATGGAGCATGTTATGTCTAAATCTTTTCAACCTACTCGTCTGGTAGGGGCTATCGCTATTGCGATGGGGTGTTCACCTGT
TATTTTTGCTGAAGATGCAACAGATGCAACTCAGCTTGACCCAATTGTTATCACTGCTTCAAAAAGTGCTGAAAAAGCAA
GTGAAGTTCCGGCTCGAATTTCTATTATTGAACCTAAAATTGTAGAACAGTCACCTATTGCTGAATTACCACACTTATTA
ATGAGTGATGCAGCTATTAATATGGTTCAAAGTGGTGGCTTAGGGCAAACATCTTCAATTTTTATTCGTGGTACTAATTC
TGAGCATGCTTTGATTTTACGTGATGGCGCACGTCTGAACACTGCCTCAACTGGTGCAGCAAATCTTGCATTTATTGATA
CCACAGATATTAAACAAATTGAAATTCTCAAAGGACCAGCATCAGTTCTTTATGGTACGGATGCTATTGGTGGTGTTGTA
CAAATTATTTCAAAGACTCCAGAAAAAACCTCAGCATTTGTGACAGGTGAAATTGGTGAAAATAAAACTTATAAATCGAT
TGTAGGCGCAGATCTTGCCGAAAACGGATTTTATGCGCAAGTTCGCGGTCAACGTTTAGAGTCTGACGGTTCACGTATCA
CTGATCTAAAAGGTAATGACATTAAAAAAGCCTCTTATGATCAAAAAGGCTTCAGCACTAAAGTTGGGGTAGAAAAAGAA
GATTTCGGGGCATCTGTTGACTACACACAAAATGAAGGAACAAGCCAATACGATACTTTTCGTTATGATGGTTCATTGAC
TTCTCAAGATTTCAAAAATGAATTACTTAATATTCGTGGCCGTGTAAATCTCAATTCAGATATTTCTCTAAATGCAAGAT
TATCTCAATTTAAAGATGAACTTGATCAAAATGGTTCAAGGGATTTTGTACATAGTACGACAAAAGAAGCAGAGGTTTAC
GGCAAATGGCAATTCACCTCATCTCAAAATGTATTGGCAGGCGTAACTCATCAAAATATTGATGGCGATGTACTCTCTTA
TGGTTCGCCTTACGATGAAGATGTCAATAGCACAGGCTATTTCGTACAACATCAATATCAGAACAATGGTTTAAATACCC
AAGTCGGTGTTCGTGTTGAAGATAACGAAAAATACGGAACACATACCGTGGCACAAGGTGCAATCCGTTATCAACTTTTA
CCATTGACGAGTATTTATGCCAACATTGGTTCAGCCTTCAAAGCACCAACACTAAATGACATGTATGGTTCTGGTGGCAA
TCCCAATCTTAAGCCAGAAGAAAGTATTTCTTATGAAGTCGGTATAGATCAAAAGTTAAATTACAATATTTCGACTGGCC
TTTCTGCCTATTACACCAAAATAGACAATCTAATTGAATCACGCTGTATAGCTGTATGTAATGGCGATTGGATAAATACA
TTCCCTGTTTATCAAAATATCAACATCGACAAGGCATCTATGCGCGGTGGTGAAATCTATGCAAATTGGAATAGAGATGA
CTTATTTATTAAATCATCTTATAACTATGTTAAAGCTATCAACGATGAAACAGATCAAGAATTATCAAGACGTCCTCGTC
AAAGCTTTACTGTAAGTACGGGTTTACAAAATGAACATTACGGACTGAGTATTTCACTTTCTGCAAAATCTAAAGCGAAA
GACTACAAACAAGATACGCCAGGATATACAACTGTTGACTTCAATGGTTACTGGAATGCCACACCAAACGTTAAGCTTTT
TACCAATATCCAGAATATTGGAGATGTGAAATATAAAACTGCTTCCTATGATAAAGGCATCTATTACGTAAATGGTGGCC
GCCTTGCATCAGCTGGTGTGACCTTAAGCTACTAA

Upstream 100 bases:

>100_bases
GTGTGGGAAGGTGAATATGTCCATAAATGAATTATGGCGCTGAAGTCCGGAGACCTACTTGTTTCATCTATCTACCCGAT
CATTCACGTGGGGTGAATGT

Downstream 100 bases:

>100_bases
GCGAAAACTTTATTATAAAAATATTTTCTTAACTCCAGTTTCGGGGTAATGTTTTGACCGACATTATCCCCTTTTTTATG
CTCAGGATTATCATCATGGG

Product: vitamin B12 receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 624; Mature: 624

Protein sequence:

>624_residues
MEHVMSKSFQPTRLVGAIAIAMGCSPVIFAEDATDATQLDPIVITASKSAEKASEVPARISIIEPKIVEQSPIAELPHLL
MSDAAINMVQSGGLGQTSSIFIRGTNSEHALILRDGARLNTASTGAANLAFIDTTDIKQIEILKGPASVLYGTDAIGGVV
QIISKTPEKTSAFVTGEIGENKTYKSIVGADLAENGFYAQVRGQRLESDGSRITDLKGNDIKKASYDQKGFSTKVGVEKE
DFGASVDYTQNEGTSQYDTFRYDGSLTSQDFKNELLNIRGRVNLNSDISLNARLSQFKDELDQNGSRDFVHSTTKEAEVY
GKWQFTSSQNVLAGVTHQNIDGDVLSYGSPYDEDVNSTGYFVQHQYQNNGLNTQVGVRVEDNEKYGTHTVAQGAIRYQLL
PLTSIYANIGSAFKAPTLNDMYGSGGNPNLKPEESISYEVGIDQKLNYNISTGLSAYYTKIDNLIESRCIAVCNGDWINT
FPVYQNINIDKASMRGGEIYANWNRDDLFIKSSYNYVKAINDETDQELSRRPRQSFTVSTGLQNEHYGLSISLSAKSKAK
DYKQDTPGYTTVDFNGYWNATPNVKLFTNIQNIGDVKYKTASYDKGIYYVNGGRLASAGVTLSY

Sequences:

>Translated_624_residues
MEHVMSKSFQPTRLVGAIAIAMGCSPVIFAEDATDATQLDPIVITASKSAEKASEVPARISIIEPKIVEQSPIAELPHLL
MSDAAINMVQSGGLGQTSSIFIRGTNSEHALILRDGARLNTASTGAANLAFIDTTDIKQIEILKGPASVLYGTDAIGGVV
QIISKTPEKTSAFVTGEIGENKTYKSIVGADLAENGFYAQVRGQRLESDGSRITDLKGNDIKKASYDQKGFSTKVGVEKE
DFGASVDYTQNEGTSQYDTFRYDGSLTSQDFKNELLNIRGRVNLNSDISLNARLSQFKDELDQNGSRDFVHSTTKEAEVY
GKWQFTSSQNVLAGVTHQNIDGDVLSYGSPYDEDVNSTGYFVQHQYQNNGLNTQVGVRVEDNEKYGTHTVAQGAIRYQLL
PLTSIYANIGSAFKAPTLNDMYGSGGNPNLKPEESISYEVGIDQKLNYNISTGLSAYYTKIDNLIESRCIAVCNGDWINT
FPVYQNINIDKASMRGGEIYANWNRDDLFIKSSYNYVKAINDETDQELSRRPRQSFTVSTGLQNEHYGLSISLSAKSKAK
DYKQDTPGYTTVDFNGYWNATPNVKLFTNIQNIGDVKYKTASYDKGIYYVNGGRLASAGVTLSY
>Mature_624_residues
MEHVMSKSFQPTRLVGAIAIAMGCSPVIFAEDATDATQLDPIVITASKSAEKASEVPARISIIEPKIVEQSPIAELPHLL
MSDAAINMVQSGGLGQTSSIFIRGTNSEHALILRDGARLNTASTGAANLAFIDTTDIKQIEILKGPASVLYGTDAIGGVV
QIISKTPEKTSAFVTGEIGENKTYKSIVGADLAENGFYAQVRGQRLESDGSRITDLKGNDIKKASYDQKGFSTKVGVEKE
DFGASVDYTQNEGTSQYDTFRYDGSLTSQDFKNELLNIRGRVNLNSDISLNARLSQFKDELDQNGSRDFVHSTTKEAEVY
GKWQFTSSQNVLAGVTHQNIDGDVLSYGSPYDEDVNSTGYFVQHQYQNNGLNTQVGVRVEDNEKYGTHTVAQGAIRYQLL
PLTSIYANIGSAFKAPTLNDMYGSGGNPNLKPEESISYEVGIDQKLNYNISTGLSAYYTKIDNLIESRCIAVCNGDWINT
FPVYQNINIDKASMRGGEIYANWNRDDLFIKSSYNYVKAINDETDQELSRRPRQSFTVSTGLQNEHYGLSISLSAKSKAK
DYKQDTPGYTTVDFNGYWNATPNVKLFTNIQNIGDVKYKTASYDKGIYYVNGGRLASAGVTLSY

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=639, Percent_Identity=27.0735524256651, Blast_Score=159, Evalue=7e-40,
Organism=Escherichia coli, GI1788478, Length=671, Percent_Identity=24.8882265275708, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1786798, Length=165, Percent_Identity=30.9090909090909, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 68375; Mature: 68375

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHVMSKSFQPTRLVGAIAIAMGCSPVIFAEDATDATQLDPIVITASKSAEKASEVPARI
CCCHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCCHHHHHCCCCEE
SIIEPKIVEQSPIAELPHLLMSDAAINMVQSGGLGQTSSIFIRGTNSEHALILRDGARLN
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEECCCEEC
TASTGAANLAFIDTTDIKQIEILKGPASVLYGTDAIGGVVQIISKTPEKTSAFVTGEIGE
CCCCCCCEEEEEECCCCCEEEEECCCHHEEECCHHHHHHHHHHHCCCCCCCEEEEECCCC
NKTYKSIVGADLAENGFYAQVRGQRLESDGSRITDLKGNDIKKASYDQKGFSTKVGVEKE
CCCHHHHHCCCHHCCCEEEEEECCEECCCCCEEEECCCCCCCCCCCCCCCCCEECCCCHH
DFGASVDYTQNEGTSQYDTFRYDGSLTSQDFKNELLNIRGRVNLNSDISLNARLSQFKDE
HCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHEEEEEEECCCCCEECHHHHHHHHH
LDQNGSRDFVHSTTKEAEVYGKWQFTSSQNVLAGVTHQNIDGDVLSYGSPYDEDVNSTGY
HHCCCCCCHHHCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCEECCCCCCCCCCCCCCE
FVQHQYQNNGLNTQVGVRVEDNEKYGTHTVAQGAIRYQLLPLTSIYANIGSAFKAPTLND
EEEEEECCCCCCEEEEEEEECCCCCCCHHHHCCCEEEEEEEHHHHHHHHCCCCCCCCCCH
MYGSGGNPNLKPEESISYEVGIDQKLNYNISTGLSAYYTKIDNLIESRCIAVCNGDWINT
HCCCCCCCCCCCCCCCEEEECCCCEECCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCEE
FPVYQNINIDKASMRGGEIYANWNRDDLFIKSSYNYVKAINDETDQELSRRPRQSFTVST
ECEEECCCCCHHHCCCCEEEEECCCCEEEEECCCCEEEECCCCHHHHHHHCCCCCEEEEC
GLQNEHYGLSISLSAKSKAKDYKQDTPGYTTVDFNGYWNATPNVKLFTNIQNIGDVKYKT
CCCCCCCCEEEEECCCHHHHHHHCCCCCEEEEEECCEECCCCCEEEEEECCCCCCEEEEE
ASYDKGIYYVNGGRLASAGVTLSY
CCCCCCEEEEECCEEEECCEEEEC
>Mature Secondary Structure
MEHVMSKSFQPTRLVGAIAIAMGCSPVIFAEDATDATQLDPIVITASKSAEKASEVPARI
CCCHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCCHHHHHCCCCEE
SIIEPKIVEQSPIAELPHLLMSDAAINMVQSGGLGQTSSIFIRGTNSEHALILRDGARLN
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEECCCEEC
TASTGAANLAFIDTTDIKQIEILKGPASVLYGTDAIGGVVQIISKTPEKTSAFVTGEIGE
CCCCCCCEEEEEECCCCCEEEEECCCHHEEECCHHHHHHHHHHHCCCCCCCEEEEECCCC
NKTYKSIVGADLAENGFYAQVRGQRLESDGSRITDLKGNDIKKASYDQKGFSTKVGVEKE
CCCHHHHHCCCHHCCCEEEEEECCEECCCCCEEEECCCCCCCCCCCCCCCCCEECCCCHH
DFGASVDYTQNEGTSQYDTFRYDGSLTSQDFKNELLNIRGRVNLNSDISLNARLSQFKDE
HCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHEEEEEEECCCCCEECHHHHHHHHH
LDQNGSRDFVHSTTKEAEVYGKWQFTSSQNVLAGVTHQNIDGDVLSYGSPYDEDVNSTGY
HHCCCCCCHHHCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCEECCCCCCCCCCCCCCE
FVQHQYQNNGLNTQVGVRVEDNEKYGTHTVAQGAIRYQLLPLTSIYANIGSAFKAPTLND
EEEEEECCCCCCEEEEEEEECCCCCCCHHHHCCCEEEEEEEHHHHHHHHCCCCCCCCCCH
MYGSGGNPNLKPEESISYEVGIDQKLNYNISTGLSAYYTKIDNLIESRCIAVCNGDWINT
HCCCCCCCCCCCCCCCEEEECCCCEECCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCEE
FPVYQNINIDKASMRGGEIYANWNRDDLFIKSSYNYVKAINDETDQELSRRPRQSFTVST
ECEEECCCCCHHHCCCCEEEEECCCCEEEEECCCCEEEECCCCHHHHHHHCCCCCEEEEC
GLQNEHYGLSISLSAKSKAKDYKQDTPGYTTVDFNGYWNATPNVKLFTNIQNIGDVKYKT
CCCCCCCCEEEEECCCHHHHHHHCCCCCEEEEEECCEECCCCCEEEEEECCCCCCEEEEE
ASYDKGIYYVNGGRLASAGVTLSY
CCCCCCEEEEECCEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA