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The map label for this gene is mscL

Identifier: 213157849

GI number: 213157849

Start: 3439379

End: 3439810

Strand: Reverse

Name: mscL

Synonym: AB57_3335

Alternate gene names: 213157849

Gene position: 3439810-3439379 (Counterclockwise)

Preceding gene: 213157850

Following gene: 213157848

Centisome position: 84.92

GC content: 35.19

Gene sequence:

>432_bases
ATGAGTATTATTCAAGAATTTAAAGAATTTGCCATTAAAGGCAATATGATGGATTTAGCTATTGGTGTAATTATCGGTGG
TGCTTTTGGTAAAATCGTAGACTCGTTAGTAAAAGATATCATCATGCCGCTTATCACTGTAATTACTGGTGGTGGTGTTG
ATTTCTCGCAGAAATTTATCGTGTTAGGTGCAAATCCTAATAACTTACAATCTCTAGATGCCCTACAAAAAGCAGGGATC
AATGTATTAACTTATGGTAACTTCCTGACCATTTTAATTAACTTCCTGATTTTGGCTTGGGTTGTGTTCTTAATGGTGAA
ATTATTAAACAAACTTCGCCGTGATAAGAATGAGCCAGAAGCTCCAGCTGCAACTCCAGAAGATATCCAATTATTACGTG
AAATTCGTGATGAGTTGAAAAAACAAGCTTAA

Upstream 100 bases:

>100_bases
AATTATCAATATCATGGCGAGTACTATCTAGACGGATAAGAGGCCAGTGAAAAAAGAAAAATATGATGTGTTCCTCCCCC
CTACATCTGGAGATGTTTAG

Downstream 100 bases:

>100_bases
TAAAAAGAATTTGAGTATAAAAAACGGTACCTAGAGTACCGTTTTTATTACATAGCATTTTATACTGGACTAGAGATATT
GATAAACTTGCTAAATCACA

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 142

Protein sequence:

>143_residues
MSIIQEFKEFAIKGNMMDLAIGVIIGGAFGKIVDSLVKDIIMPLITVITGGGVDFSQKFIVLGANPNNLQSLDALQKAGI
NVLTYGNFLTILINFLILAWVVFLMVKLLNKLRRDKNEPEAPAATPEDIQLLREIRDELKKQA

Sequences:

>Translated_143_residues
MSIIQEFKEFAIKGNMMDLAIGVIIGGAFGKIVDSLVKDIIMPLITVITGGGVDFSQKFIVLGANPNNLQSLDALQKAGI
NVLTYGNFLTILINFLILAWVVFLMVKLLNKLRRDKNEPEAPAATPEDIQLLREIRDELKKQA
>Mature_142_residues
SIIQEFKEFAIKGNMMDLAIGVIIGGAFGKIVDSLVKDIIMPLITVITGGGVDFSQKFIVLGANPNNLQSLDALQKAGIN
VLTYGNFLTILINFLILAWVVFLMVKLLNKLRRDKNEPEAPAATPEDIQLLREIRDELKKQA

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=143, Percent_Identity=49.6503496503497, Blast_Score=124, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_ACIB3 (B7GWU1)

Other databases:

- EMBL:   CP001172
- RefSeq:   YP_002324552.1
- ProteinModelPortal:   B7GWU1
- SMR:   B7GWU1
- GeneID:   7057968
- GenomeReviews:   CP001172_GR
- KEGG:   abb:ABBFA_000628
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- ProtClustDB:   PRK13952
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15783; Mature: 15652

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x235ad8dc)-; HASH(0x230c0b40)-; HASH(0x236d4700)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIQEFKEFAIKGNMMDLAIGVIIGGAFGKIVDSLVKDIIMPLITVITGGGVDFSQKFI
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
VLGANPNNLQSLDALQKAGINVLTYGNFLTILINFLILAWVVFLMVKLLNKLRRDKNEPE
EECCCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
APAATPEDIQLLREIRDELKKQA
CCCCCHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
SIIQEFKEFAIKGNMMDLAIGVIIGGAFGKIVDSLVKDIIMPLITVITGGGVDFSQKFI
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
VLGANPNNLQSLDALQKAGINVLTYGNFLTILINFLILAWVVFLMVKLLNKLRRDKNEPE
EECCCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
APAATPEDIQLLREIRDELKKQA
CCCCCHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA