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The map label for this gene is proS

Identifier: 213157835

GI number: 213157835

Start: 3426111

End: 3427826

Strand: Direct

Name: proS

Synonym: AB57_3319

Alternate gene names: 213157835

Gene position: 3426111-3427826 (Clockwise)

Preceding gene: 213157834

Following gene: 213157840

Centisome position: 84.58

GC content: 42.19

Gene sequence:

>1716_bases
ATGCGCGCAAGCCGCTTTTTATTTGCAACGTTGAGAGAAACCCCAAACGATGCTGAAGTGATTTCACATCAGCTCATGTT
ACGTGCGGGGATGATTCGTAAATTGGCATCTGGTTTATATACATGGCTGCCGATGGGTACACGTGTGCTTAAAAAAGTTG
ATGCCATTGTTCGTGAAGAAATGAACCGTTCAGGTGCTATGGAAGTGTTCATGCCCGTAACTCAACCCGCAAGCTTGTGG
GAAGAATCGGGTCGTTATGAACAATATGGTCCTGAATTATTACGTTTCAAAGATCGTCACGACAATCCGTTTGTGCTTGG
TCCTACACATGAAGAAGTGATTACCGATTTAGCACGTAACGAGCTAAAAAGTTATAAACAGCTTCCTGTAAATTTCTATC
AAATTCAAACCAAATTCCGTGATGAGATCCGCCCTCGTTTTGGTGTAATGCGTTCACGCGAGTTCATCATGAAAGATGCT
TATTCTTTCCATGCAACTCAAGAATCATTACAAGAAACTTATGATGTGATGTATGACACCTATAGCCGTATTTTCACTCG
TTTAGGTTTAGATTTCCGCCCAGTACAAGCAGATACAGGTTCAATTGGTGGTTCAGCTTCACATGAATTCCACGTATTAG
CTGCAAGTGGTGAAGATGACATTGCATTTTCAACTGAGTCTGATTATGCAGCAAACGTTGAAATGGCTGAAGCTGTTTTA
GTGGGTGAGCGTGCAGCGCCTACACAAGAATTTAAATTGGTTGAAACACCAAATCAAAAAACAATTGCTGATGTTTGCCA
GTTCTTAAATGCTGACCCTAAACAATCTGTAAAAGCCTTATTGGTTCAAGGCGTTGCTGATGAGAAAGGCAATGTACCTG
TCGTTGCCCTATTCTTACGTGGTGACCACGAACTGAATGAAATTAAAGCCGAGAAACATCCTTTAGTGGCCGCTCCTCTC
GCATTTGCCACAGAAGAACAACTTCAAGCATTTGGCTTAACTGCTGGCTTCACTGGTCCACAAGGTTTAGTTGAAAAAGG
CATCACTGTAATTGTTGACCGTGCTGCATCTGTTCTTTCTGACTTCGTTGCTGGTGCAAATGAGGCAGACAAACATGCTA
TAGGCGTAAACTGGGAACGTGATGCACAAATTACTGAAGTTTTCGATTTGCGTAATGTGGTTGAAGGTGACCCATCTCCA
GATGGTAAAGGTACACTACAAATTAAACGCGGTATTGAAGTGGGCCATATTTTCCAACTTGGTACTAAATACTCTGAAGC
TTTAGGATGTAAAGTTCTCGGTGAAGATGGCAAACCATTTACAGTAACAATGGGCTGTTATGGTATTGGTGTGACACGCG
TAGTTGCAGCTGCAATTGAACAAAATTACGATGACAAAGGGATTATTTGGCCACAAGCGATTGCTCCTTTTGAAATCGCA
ATTGTTCCAATGAATGCACACAAGTCACCACGTACTCTTGAAGCAGCAGAAGCGCTTTATGCAGAACTTCAAGCCCAAGG
CTTTGACGTATTACTTGATGACCGTAACGAACGTCCGGGCGTTAAATTCTCTGACCTTGAACTAATGGGTATTCCACACC
GTATTGTGATTGGTGAAAAAGGTCTTGATGCGGGTACTTTTGAATATAAAGGTCGCCGTGATGCTGAAGCGAGCAATTTA
ACTAAAGAAGAACTTTTAGCTAAATTAGCGCGTTAA

Upstream 100 bases:

>100_bases
AAAAACTTTATGCTGCGTCAAACCCGACGATTCATGTCATAATGCAGCGACTATCGTATAATCCGCGTTTTTATAACGCT
TAACTTTTAGGACTTTTTCC

Downstream 100 bases:

>100_bases
TAAATACATAAATAAAAAAGCAGCTTTTAGAAGCTGCTTTTTTAATTTAATTTTACGCCTGAACTGTTAAGTCTTTAATG
GTTGTGGAAGATCCATTGTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MRASRFLFATLRETPNDAEVISHQLMLRAGMIRKLASGLYTWLPMGTRVLKKVDAIVREEMNRSGAMEVFMPVTQPASLW
EESGRYEQYGPELLRFKDRHDNPFVLGPTHEEVITDLARNELKSYKQLPVNFYQIQTKFRDEIRPRFGVMRSREFIMKDA
YSFHATQESLQETYDVMYDTYSRIFTRLGLDFRPVQADTGSIGGSASHEFHVLAASGEDDIAFSTESDYAANVEMAEAVL
VGERAAPTQEFKLVETPNQKTIADVCQFLNADPKQSVKALLVQGVADEKGNVPVVALFLRGDHELNEIKAEKHPLVAAPL
AFATEEQLQAFGLTAGFTGPQGLVEKGITVIVDRAASVLSDFVAGANEADKHAIGVNWERDAQITEVFDLRNVVEGDPSP
DGKGTLQIKRGIEVGHIFQLGTKYSEALGCKVLGEDGKPFTVTMGCYGIGVTRVVAAAIEQNYDDKGIIWPQAIAPFEIA
IVPMNAHKSPRTLEAAEALYAELQAQGFDVLLDDRNERPGVKFSDLELMGIPHRIVIGEKGLDAGTFEYKGRRDAEASNL
TKEELLAKLAR

Sequences:

>Translated_571_residues
MRASRFLFATLRETPNDAEVISHQLMLRAGMIRKLASGLYTWLPMGTRVLKKVDAIVREEMNRSGAMEVFMPVTQPASLW
EESGRYEQYGPELLRFKDRHDNPFVLGPTHEEVITDLARNELKSYKQLPVNFYQIQTKFRDEIRPRFGVMRSREFIMKDA
YSFHATQESLQETYDVMYDTYSRIFTRLGLDFRPVQADTGSIGGSASHEFHVLAASGEDDIAFSTESDYAANVEMAEAVL
VGERAAPTQEFKLVETPNQKTIADVCQFLNADPKQSVKALLVQGVADEKGNVPVVALFLRGDHELNEIKAEKHPLVAAPL
AFATEEQLQAFGLTAGFTGPQGLVEKGITVIVDRAASVLSDFVAGANEADKHAIGVNWERDAQITEVFDLRNVVEGDPSP
DGKGTLQIKRGIEVGHIFQLGTKYSEALGCKVLGEDGKPFTVTMGCYGIGVTRVVAAAIEQNYDDKGIIWPQAIAPFEIA
IVPMNAHKSPRTLEAAEALYAELQAQGFDVLLDDRNERPGVKFSDLELMGIPHRIVIGEKGLDAGTFEYKGRRDAEASNL
TKEELLAKLAR
>Mature_571_residues
MRASRFLFATLRETPNDAEVISHQLMLRAGMIRKLASGLYTWLPMGTRVLKKVDAIVREEMNRSGAMEVFMPVTQPASLW
EESGRYEQYGPELLRFKDRHDNPFVLGPTHEEVITDLARNELKSYKQLPVNFYQIQTKFRDEIRPRFGVMRSREFIMKDA
YSFHATQESLQETYDVMYDTYSRIFTRLGLDFRPVQADTGSIGGSASHEFHVLAASGEDDIAFSTESDYAANVEMAEAVL
VGERAAPTQEFKLVETPNQKTIADVCQFLNADPKQSVKALLVQGVADEKGNVPVVALFLRGDHELNEIKAEKHPLVAAPL
AFATEEQLQAFGLTAGFTGPQGLVEKGITVIVDRAASVLSDFVAGANEADKHAIGVNWERDAQITEVFDLRNVVEGDPSP
DGKGTLQIKRGIEVGHIFQLGTKYSEALGCKVLGEDGKPFTVTMGCYGIGVTRVVAAAIEQNYDDKGIIWPQAIAPFEIA
IVPMNAHKSPRTLEAAEALYAELQAQGFDVLLDDRNERPGVKFSDLELMGIPHRIVIGEKGLDAGTFEYKGRRDAEASNL
TKEELLAKLAR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=235, Percent_Identity=44.2553191489362, Blast_Score=215, Evalue=1e-55,
Organism=Escherichia coli, GI1786392, Length=572, Percent_Identity=68.1818181818182, Blast_Score=797, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=216, Percent_Identity=41.2037037037037, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=38.2022471910112, Blast_Score=82, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=218, Percent_Identity=38.5321100917431, Blast_Score=174, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24656200, Length=245, Percent_Identity=36.734693877551, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_ACIB3 (B7GWV6)

Other databases:

- EMBL:   CP001172
- RefSeq:   YP_002324567.1
- ProteinModelPortal:   B7GWV6
- SMR:   B7GWV6
- GeneID:   7057551
- GenomeReviews:   CP001172_GR
- KEGG:   abb:ABBFA_000643
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63276; Mature: 63276

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRASRFLFATLRETPNDAEVISHQLMLRAGMIRKLASGLYTWLPMGTRVLKKVDAIVREE
CCCCCEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
MNRSGAMEVFMPVTQPASLWEESGRYEQYGPELLRFKDRHDNPFVLGPTHEEVITDLARN
HCCCCCEEEEEECCCCHHHHHHCCCCHHCCCHHHHHHCCCCCCEEECCCHHHHHHHHHHH
ELKSYKQLPVNFYQIQTKFRDEIRPRFGVMRSREFIMKDAYSFHATQESLQETYDVMYDT
HHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSRIFTRLGLDFRPVQADTGSIGGSASHEFHVLAASGEDDIAFSTESDYAANVEMAEAVL
HHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCHHHHHHHH
VGERAAPTQEFKLVETPNQKTIADVCQFLNADPKQSVKALLVQGVADEKGNVPVVALFLR
CCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEE
GDHELNEIKAEKHPLVAAPLAFATEEQLQAFGLTAGFTGPQGLVEKGITVIVDRAASVLS
CCCHHHHHHHCCCCCEECCHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHEEHHHHHHHHH
DFVAGANEADKHAIGVNWERDAQITEVFDLRNVVEGDPSPDGKGTLQIKRGIEVGHIFQL
HHHCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEHHHHH
GTKYSEALGCKVLGEDGKPFTVTMGCYGIGVTRVVAAAIEQNYDDKGIIWPQAIAPFEIA
CCHHHHHCCCEEECCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
IVPMNAHKSPRTLEAAEALYAELQAQGFDVLLDDRNERPGVKFSDLELMGIPHRIVIGEK
EEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEECCEEEECCCEEEEEECC
GLDAGTFEYKGRRDAEASNLTKEELLAKLAR
CCCCCCCCCCCCCCCCHHCCCHHHHHHHHCC
>Mature Secondary Structure
MRASRFLFATLRETPNDAEVISHQLMLRAGMIRKLASGLYTWLPMGTRVLKKVDAIVREE
CCCCCEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
MNRSGAMEVFMPVTQPASLWEESGRYEQYGPELLRFKDRHDNPFVLGPTHEEVITDLARN
HCCCCCEEEEEECCCCHHHHHHCCCCHHCCCHHHHHHCCCCCCEEECCCHHHHHHHHHHH
ELKSYKQLPVNFYQIQTKFRDEIRPRFGVMRSREFIMKDAYSFHATQESLQETYDVMYDT
HHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSRIFTRLGLDFRPVQADTGSIGGSASHEFHVLAASGEDDIAFSTESDYAANVEMAEAVL
HHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCHHHHHHHH
VGERAAPTQEFKLVETPNQKTIADVCQFLNADPKQSVKALLVQGVADEKGNVPVVALFLR
CCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEE
GDHELNEIKAEKHPLVAAPLAFATEEQLQAFGLTAGFTGPQGLVEKGITVIVDRAASVLS
CCCHHHHHHHCCCCCEECCHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHEEHHHHHHHHH
DFVAGANEADKHAIGVNWERDAQITEVFDLRNVVEGDPSPDGKGTLQIKRGIEVGHIFQL
HHHCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEHHHHH
GTKYSEALGCKVLGEDGKPFTVTMGCYGIGVTRVVAAAIEQNYDDKGIIWPQAIAPFEIA
CCHHHHHCCCEEECCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
IVPMNAHKSPRTLEAAEALYAELQAQGFDVLLDDRNERPGVKFSDLELMGIPHRIVIGEK
EEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEECCEEEECCCEEEEEECC
GLDAGTFEYKGRRDAEASNLTKEELLAKLAR
CCCCCCCCCCCCCCCCHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA