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The map label for this gene is dapE [H]

Identifier: 213157823

GI number: 213157823

Start: 3408412

End: 3409545

Strand: Direct

Name: dapE [H]

Synonym: AB57_3307

Alternate gene names: 213157823

Gene position: 3408412-3409545 (Clockwise)

Preceding gene: 213157822

Following gene: 213157832

Centisome position: 84.15

GC content: 40.48

Gene sequence:

>1134_bases
ATGAACCACTCAGACACTCTCTCGCTTAGCTTAGAACTTTTAGAACAGCCTTCTGTTACCCCTATTGATCATACTTGCCA
AACTATTATGGCTGACCGCTTAGCTAAAGTTGGCTTCCATATTGAACCTATGCGCTTTGGAGATGTCGATAATTTATGGG
CTCGACGTGGAACCGAAGGCCCTGTATTTTGTTTTGCTGGTCATACCGACGTAGTACCAACAGGACGTTTAGACGCTTGG
AATTCAGACCCATTTGCACCAGAAATTCGCGATGGCAAATTATATGGTCGTGGTTCAGCAGATATGAAAACTGCATTAGC
TGCAATGGTAGTTGCATCTGAACGTTTTGTAGCAAAACACCCAAACCATAAAGGTTCAATTGCTTTTTTAATTACTTCAG
ATGAAGAAGGTCCTGCTGTTAACGGTACCGTTAAAGTCATCGAAACACTGGAAAAGCGTAATGAAAAAATTACTTGGTGT
TTGGTTGGTGAACCATCAAGTACTCATAAATTAGGTGATATTGTTAAGAATGGCCGTCGTGGTTCTCTTAATGCTGTATT
GAAGGTTCAAGGCAAACAAGGTCATGTAGCTTACCCACACTTGGCACGTAATCCAATTCATGAAGCATCTCCAGCGTTAG
CTGAGTTGTGCCAAACAGTTTGGGACAATGGCAATGAGTATTTCCCTGCAACTTCATTCCAGATTTCAAATATCCATGCA
GGTACTGGCGCAACCAATGTAATCCCTGGTGCTTTAGAAGTTACTTTTAATTTCCGTTATTCAACCGAGGTTACAGCAGA
ACAGCTTAAACAACGTGTTCATGAAATTTTAGACAAGCATGGCTTGCAGTATGAAATTGTCTGGAATTTGTCTGGCTTAC
CATTCTTAACTCCAGTAGGTGAATTAGTTAACGCAGCTCAAACTGCTATTTTAAATGTGACTGGTACTGAGACTGAGCTT
TCAACAAGTGGTGGTACATCTGATGGACGCTTCATTGCTCCAACAGGTGCACAAGTTTTAGAACTCGGTGTTCTAAACGC
TACTATCCATCAAATTAATGAACATGTTGATGTACATGATCTTGATCCATTAACTGATATTTATGAGCAAATTTTAGAGA
ACCTACTTGCTTAA

Upstream 100 bases:

>100_bases
TATCGTTTCGATAAGGTTTTTTATTTTTAAATACCCATCCCTTAGAGATTCGCCTATTATATCGGACTATATTTCACCTC
ATCAGTACGCAATTTCCGGT

Downstream 100 bases:

>100_bases
AATATTAAAGATCAGGAATTAGATAAAAAGCATCCTCTTTAGGGTGCTTTTTATTATTTTTAACTGTGAGAATATTTGTT
AAAAATCTTATAATAAGATT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MNHSDTLSLSLELLEQPSVTPIDHTCQTIMADRLAKVGFHIEPMRFGDVDNLWARRGTEGPVFCFAGHTDVVPTGRLDAW
NSDPFAPEIRDGKLYGRGSADMKTALAAMVVASERFVAKHPNHKGSIAFLITSDEEGPAVNGTVKVIETLEKRNEKITWC
LVGEPSSTHKLGDIVKNGRRGSLNAVLKVQGKQGHVAYPHLARNPIHEASPALAELCQTVWDNGNEYFPATSFQISNIHA
GTGATNVIPGALEVTFNFRYSTEVTAEQLKQRVHEILDKHGLQYEIVWNLSGLPFLTPVGELVNAAQTAILNVTGTETEL
STSGGTSDGRFIAPTGAQVLELGVLNATIHQINEHVDVHDLDPLTDIYEQILENLLA

Sequences:

>Translated_377_residues
MNHSDTLSLSLELLEQPSVTPIDHTCQTIMADRLAKVGFHIEPMRFGDVDNLWARRGTEGPVFCFAGHTDVVPTGRLDAW
NSDPFAPEIRDGKLYGRGSADMKTALAAMVVASERFVAKHPNHKGSIAFLITSDEEGPAVNGTVKVIETLEKRNEKITWC
LVGEPSSTHKLGDIVKNGRRGSLNAVLKVQGKQGHVAYPHLARNPIHEASPALAELCQTVWDNGNEYFPATSFQISNIHA
GTGATNVIPGALEVTFNFRYSTEVTAEQLKQRVHEILDKHGLQYEIVWNLSGLPFLTPVGELVNAAQTAILNVTGTETEL
STSGGTSDGRFIAPTGAQVLELGVLNATIHQINEHVDVHDLDPLTDIYEQILENLLA
>Mature_377_residues
MNHSDTLSLSLELLEQPSVTPIDHTCQTIMADRLAKVGFHIEPMRFGDVDNLWARRGTEGPVFCFAGHTDVVPTGRLDAW
NSDPFAPEIRDGKLYGRGSADMKTALAAMVVASERFVAKHPNHKGSIAFLITSDEEGPAVNGTVKVIETLEKRNEKITWC
LVGEPSSTHKLGDIVKNGRRGSLNAVLKVQGKQGHVAYPHLARNPIHEASPALAELCQTVWDNGNEYFPATSFQISNIHA
GTGATNVIPGALEVTFNFRYSTEVTAEQLKQRVHEILDKHGLQYEIVWNLSGLPFLTPVGELVNAAQTAILNVTGTETEL
STSGGTSDGRFIAPTGAQVLELGVLNATIHQINEHVDVHDLDPLTDIYEQILENLLA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=372, Percent_Identity=58.0645161290323, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1790395, Length=262, Percent_Identity=28.2442748091603, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 41063; Mature: 41063

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSDTLSLSLELLEQPSVTPIDHTCQTIMADRLAKVGFHIEPMRFGDVDNLWARRGTEG
CCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHCCCCC
PVFCFAGHTDVVPTGRLDAWNSDPFAPEIRDGKLYGRGSADMKTALAAMVVASERFVAKH
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCC
PNHKGSIAFLITSDEEGPAVNGTVKVIETLEKRNEKITWCLVGEPSSTHKLGDIVKNGRR
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCC
GSLNAVLKVQGKQGHVAYPHLARNPIHEASPALAELCQTVWDNGNEYFPATSFQISNIHA
CCCEEEEEEECCCCCEECCHHHCCCCCCCCHHHHHHHHHHHCCCCEECCCCCEEEEEEEC
GTGATNVIPGALEVTFNFRYSTEVTAEQLKQRVHEILDKHGLQYEIVWNLSGLPFLTPVG
CCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHH
ELVNAAQTAILNVTGTETELSTSGGTSDGRFIAPTGAQVLELGVLNATIHQINEHVDVHD
HHHHHHHHEEEEECCCCEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCC
LDPLTDIYEQILENLLA
CCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNHSDTLSLSLELLEQPSVTPIDHTCQTIMADRLAKVGFHIEPMRFGDVDNLWARRGTEG
CCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHCCCCC
PVFCFAGHTDVVPTGRLDAWNSDPFAPEIRDGKLYGRGSADMKTALAAMVVASERFVAKH
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCC
PNHKGSIAFLITSDEEGPAVNGTVKVIETLEKRNEKITWCLVGEPSSTHKLGDIVKNGRR
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCC
GSLNAVLKVQGKQGHVAYPHLARNPIHEASPALAELCQTVWDNGNEYFPATSFQISNIHA
CCCEEEEEEECCCCCEECCHHHCCCCCCCCHHHHHHHHHHHCCCCEECCCCCEEEEEEEC
GTGATNVIPGALEVTFNFRYSTEVTAEQLKQRVHEILDKHGLQYEIVWNLSGLPFLTPVG
CCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHH
ELVNAAQTAILNVTGTETELSTSGGTSDGRFIAPTGAQVLELGVLNATIHQINEHVDVHD
HHHHHHHHEEEEECCCCEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCC
LDPLTDIYEQILENLLA
CCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA