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The map label for this gene is ydgK [H]

Identifier: 213157814

GI number: 213157814

Start: 3397125

End: 3398339

Strand: Reverse

Name: ydgK [H]

Synonym: AB57_3298

Alternate gene names: 213157814

Gene position: 3398339-3397125 (Counterclockwise)

Preceding gene: 213157819

Following gene: 213157812

Centisome position: 83.9

GC content: 41.73

Gene sequence:

>1215_bases
ATGTCTGAACAAACAGCCCAGCGTCAATATTCTATTGGCTGGATTATGTTGCTCGCTTTACTCACGGCTTTAGGGCCATT
ATCCATTGATATGTACTTACCTGCGTTGCCTCAAATGGCCCATGATTTTGGGGTGAGTACACAAATGGTAGCAAACACAT
TGCCGGCTTATTTTTTTGGTTTAGCCATTGGACAACTGGTATATGGACCTTTAAGCGACCGTATTGGTCGAAAAAAGCCT
CTTTATTTTGGGCTTGCGCTTTATGCAGTTGCAAGCTTGTTCTGTGTGCTCGCTACAAATGAATGGAGCCTAATTGCTGC
CCGTATTTTACAAGCCTTAGGTGGTTGTGTGGGGGTGGTTATGGCTCGTGCTGCTATACGAGATAGATTAGATGTCCAAG
GCTCGGCACAAGCTTTTTCAAGCATGATGATCGTAATGGGCTTAGCACCTATTTTGGCCCCGATGATTGGGGCCTGGATA
TTAATCTGGTTTCCGTGGCAAGCGATTTTTATTGCGCTTTCAATTGTAGGGGCAATCTGTTGGCTATGTGTACATTTCTT
TTTTAAAGAAACTTTGGCAGTTGATAAGAGACTCAAGTTATCTCTATATCAAGTGGCTACCTTATATGGTGCTATTTTCA
AAGATGCAAGCTTCCGTTTACCTATGTTTGCAGGATGTTTAACAGGTGCAGCATTATTCTGTTATATCAGTTCAGCTCCA
GCCGTTTTTATGGACCAATATGGCTTAAATCAGCAAGAGTTTGCTTATGTATTCGGGCTCAATGCTTTTGGCATTATGTT
AATGTCATCTTTAAATAAGCACTTAACCACACGTGTTGAAATCACTAAGCGCTTAAAAGCAGGTTCAATGGTACAAGTGA
CAGGTGCAAGCATTGTGTTGATTGCTGGCTTAATTTCTGCAGCACCTTTATGGCTAGTAATGTTAGGACTGTTCTTAGCA
ATTTCAGGAATTGGTTTAACTGGACCAAATGCTATGGCTCTAGCCATGTCAAAGCAAGGTGCTCGTGCAGGAACAGCCAG
TGCAATTATGGGCAGTATGCAATTTGCTTGCGGTCTATTGGGTGGAGTACTGCTTAACTTCCTTATTTGGTCTGCATCAC
TCAATATGGGAGTGATGATGGTGTTATTTACATTAAGTGGTTTTATTGTTGTTTTAAAAGCGATTCGACAAGCAAAAAGC
CAATCACTCTCTTGA

Upstream 100 bases:

>100_bases
GATAAAGGATTTGCGTATTTCTACACCAATGTTGCACAAATCATACGATAGACAGTGTAAGCTTGTCTGTATGCAAATGA
TTGAAAAATGAAATATCGTT

Downstream 100 bases:

>100_bases
TTTTAAGAGAGTGATTGTCTTGCATCTTTAATCAAGAAAAACTGCAAAATTCTCAACAGGCTCTCTAGGTGTAATAAAGG
TATTCACAATATGTTCAGGG

Product: drug resistance transporter, Bcr/CflA subfamily

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSEQTAQRQYSIGWIMLLALLTALGPLSIDMYLPALPQMAHDFGVSTQMVANTLPAYFFGLAIGQLVYGPLSDRIGRKKP
LYFGLALYAVASLFCVLATNEWSLIAARILQALGGCVGVVMARAAIRDRLDVQGSAQAFSSMMIVMGLAPILAPMIGAWI
LIWFPWQAIFIALSIVGAICWLCVHFFFKETLAVDKRLKLSLYQVATLYGAIFKDASFRLPMFAGCLTGAALFCYISSAP
AVFMDQYGLNQQEFAYVFGLNAFGIMLMSSLNKHLTTRVEITKRLKAGSMVQVTGASIVLIAGLISAAPLWLVMLGLFLA
ISGIGLTGPNAMALAMSKQGARAGTASAIMGSMQFACGLLGGVLLNFLIWSASLNMGVMMVLFTLSGFIVVLKAIRQAKS
QSLS

Sequences:

>Translated_404_residues
MSEQTAQRQYSIGWIMLLALLTALGPLSIDMYLPALPQMAHDFGVSTQMVANTLPAYFFGLAIGQLVYGPLSDRIGRKKP
LYFGLALYAVASLFCVLATNEWSLIAARILQALGGCVGVVMARAAIRDRLDVQGSAQAFSSMMIVMGLAPILAPMIGAWI
LIWFPWQAIFIALSIVGAICWLCVHFFFKETLAVDKRLKLSLYQVATLYGAIFKDASFRLPMFAGCLTGAALFCYISSAP
AVFMDQYGLNQQEFAYVFGLNAFGIMLMSSLNKHLTTRVEITKRLKAGSMVQVTGASIVLIAGLISAAPLWLVMLGLFLA
ISGIGLTGPNAMALAMSKQGARAGTASAIMGSMQFACGLLGGVLLNFLIWSASLNMGVMMVLFTLSGFIVVLKAIRQAKS
QSLS
>Mature_403_residues
SEQTAQRQYSIGWIMLLALLTALGPLSIDMYLPALPQMAHDFGVSTQMVANTLPAYFFGLAIGQLVYGPLSDRIGRKKPL
YFGLALYAVASLFCVLATNEWSLIAARILQALGGCVGVVMARAAIRDRLDVQGSAQAFSSMMIVMGLAPILAPMIGAWIL
IWFPWQAIFIALSIVGAICWLCVHFFFKETLAVDKRLKLSLYQVATLYGAIFKDASFRLPMFAGCLTGAALFCYISSAPA
VFMDQYGLNQQEFAYVFGLNAFGIMLMSSLNKHLTTRVEITKRLKAGSMVQVTGASIVLIAGLISAAPLWLVMLGLFLAI
SGIGLTGPNAMALAMSKQGARAGTASAIMGSMQFACGLLGGVLLNFLIWSASLNMGVMMVLFTLSGFIVVLKAIRQAKSQ
SLS

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=398, Percent_Identity=30.1507537688442, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1790146, Length=343, Percent_Identity=30.0291545189504, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI48994889, Length=230, Percent_Identity=31.7391304347826, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787065, Length=331, Percent_Identity=27.190332326284, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI87082312, Length=363, Percent_Identity=29.4765840220386, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1790794, Length=329, Percent_Identity=22.7963525835866, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43401; Mature: 43270

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SEQTAQRQYSIGWIMLLALLTALGPLSIDMYLPALPQMAHDFGVSTQMVANTLPAYFFG
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHH
LAIGQLVYGPLSDRIGRKKPLYFGLALYAVASLFCVLATNEWSLIAARILQALGGCVGVV
HHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
MARAAIRDRLDVQGSAQAFSSMMIVMGLAPILAPMIGAWILIWFPWQAIFIALSIVGAIC
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLCVHFFFKETLAVDKRLKLSLYQVATLYGAIFKDASFRLPMFAGCLTGAALFCYISSAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
AVFMDQYGLNQQEFAYVFGLNAFGIMLMSSLNKHLTTRVEITKRLKAGSMVQVTGASIVL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHH
IAGLISAAPLWLVMLGLFLAISGIGLTGPNAMALAMSKQGARAGTASAIMGSMQFACGLL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEHHCCCCCCHHHHHHHHHHHHHHHH
GGVLLNFLIWSASLNMGVMMVLFTLSGFIVVLKAIRQAKSQSLS
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]