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The map label for this gene is dnaB [H]

Identifier: 213157767

GI number: 213157767

Start: 3353067

End: 3354392

Strand: Reverse

Name: dnaB [H]

Synonym: AB57_3246

Alternate gene names: 213157767

Gene position: 3354392-3353067 (Counterclockwise)

Preceding gene: 213157768

Following gene: 213157766

Centisome position: 82.81

GC content: 41.33

Gene sequence:

>1326_bases
ATGAGTTCTGATATCCAAAATATTTCTATTGAGCAGAGTGTTCTTGTTGCACTCATGACAACAAGCAACTCACTTGAAGT
AGTTGCAAACGATTTGACTGAGGAACATTTCTTCGCTGGACGACACAAAATCATTTATCGCGCCATTGTTGAGTTATCAA
ATGCTGATATGCCGTATGACGCAGTATTCGTTGGCAAGCATCTACAAGAAAGAAATCTGCTTAACGATATCGGCGGTGAA
GAATATTTAATTCAACTTAATAGCGCTATTGGTAGTGTCCATCACCTTGAATACTTTGTCGCAGAATTAACTAAGTTAAA
AAACCATCGTGAAGTTGAAGGTATTGGTTTAGCTATTGCTGGACGCGCTAAAGACTTAACAGTAAGTGACATTTACCTTG
AAGCTGAGAACCTATTCAGCACTTCAAGCAGCACCTTAGAGGCTAAGCAGACTAGTTTCGATTTCAATCAAGCACTAGAA
AAAACTCTTGAAAGATTCGAAAAGAAAATTGCTCAAAAAGAAGATAAGGGGTTCATTGGTGTTCAGTTCAATATTCCACA
TTTAGATAATCTTCTTGGGACTATCGAGAAAGGACATTTTTGCGTTATTGGTGGTCGTCCGGGTAGTGGCAAGTCAACAC
TCGCGCAAATGTGTGCAATGCAAACTGCTAAGCGCTACAACATGCCTGTTTTATTTATCTCTGCTGAGATGGACACACCA
ACCCTAACCAACCGCATGATCTCAGCATTAGGGCATATCCCATATAACAATCTGCACAATGGGGAAATTTATGACGGGAT
GTTTGAAAAGCTTACTGGCACGATAGCTCAGTTCCGCAACCTTCCAATTTTTATTGAAGAGAAGCAGAAGCCAACAATTT
CTGAAATCCAAAGCTATGCGCGTAAAGCAAAACGCAAATATAAGGCTCTAGGCTGCATCATTGTGGACTACTTGGGCTTA
ATTCGTGACCCATCTAAAAAAGACCGTGTTCAGGAAGTTGCATCAATTAGCCGTGATTTAAAAGCCATGGCTAAAGAGTT
TGATTGTCCAGTAATTGCATTGGCTCAACTTAACCGTGGAGCAGAAGGACACAAGCCAGTAGCAAGTGATCTTAAGGATT
CTGGACAGATTGAACAGGATGCAGACCAAATCATCATGGTTCATCCAATCCTCGAAAAAGAGACTAATGCGCCAACTGGT
GTAACCGAGTTAATTATTGCCAAAAACCGTCATGGCAAGCGTGGATCTGTGAATGTTCAAGACCGTTTAGATATTTGCCG
TTTCGTTGGGATGTCATTCCCAGTGGAAGAGAGAGGTGCGGCGTGA

Upstream 100 bases:

>100_bases
GTTACAACTGGAAGCCAGCATTTGAAACTTTGAATGGTTCTGCACAACAACAAACACCAGCAGATATGAAAAATGCTGAT
CTTAATTATGGAGATTGGTA

Downstream 100 bases:

>100_bases
ATCCAAAACAAAGAGTAATCGCGTTCCAAAACATTTTTGACATTTTGTTGTTCGCAACGCATGCGACTGAGCCTTTCACA
ATGAAGGATTTACGTGACTA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSSDIQNISIEQSVLVALMTTSNSLEVVANDLTEEHFFAGRHKIIYRAIVELSNADMPYDAVFVGKHLQERNLLNDIGGE
EYLIQLNSAIGSVHHLEYFVAELTKLKNHREVEGIGLAIAGRAKDLTVSDIYLEAENLFSTSSSTLEAKQTSFDFNQALE
KTLERFEKKIAQKEDKGFIGVQFNIPHLDNLLGTIEKGHFCVIGGRPGSGKSTLAQMCAMQTAKRYNMPVLFISAEMDTP
TLTNRMISALGHIPYNNLHNGEIYDGMFEKLTGTIAQFRNLPIFIEEKQKPTISEIQSYARKAKRKYKALGCIIVDYLGL
IRDPSKKDRVQEVASISRDLKAMAKEFDCPVIALAQLNRGAEGHKPVASDLKDSGQIEQDADQIIMVHPILEKETNAPTG
VTELIIAKNRHGKRGSVNVQDRLDICRFVGMSFPVEERGAA

Sequences:

>Translated_441_residues
MSSDIQNISIEQSVLVALMTTSNSLEVVANDLTEEHFFAGRHKIIYRAIVELSNADMPYDAVFVGKHLQERNLLNDIGGE
EYLIQLNSAIGSVHHLEYFVAELTKLKNHREVEGIGLAIAGRAKDLTVSDIYLEAENLFSTSSSTLEAKQTSFDFNQALE
KTLERFEKKIAQKEDKGFIGVQFNIPHLDNLLGTIEKGHFCVIGGRPGSGKSTLAQMCAMQTAKRYNMPVLFISAEMDTP
TLTNRMISALGHIPYNNLHNGEIYDGMFEKLTGTIAQFRNLPIFIEEKQKPTISEIQSYARKAKRKYKALGCIIVDYLGL
IRDPSKKDRVQEVASISRDLKAMAKEFDCPVIALAQLNRGAEGHKPVASDLKDSGQIEQDADQIIMVHPILEKETNAPTG
VTELIIAKNRHGKRGSVNVQDRLDICRFVGMSFPVEERGAA
>Mature_440_residues
SSDIQNISIEQSVLVALMTTSNSLEVVANDLTEEHFFAGRHKIIYRAIVELSNADMPYDAVFVGKHLQERNLLNDIGGEE
YLIQLNSAIGSVHHLEYFVAELTKLKNHREVEGIGLAIAGRAKDLTVSDIYLEAENLFSTSSSTLEAKQTSFDFNQALEK
TLERFEKKIAQKEDKGFIGVQFNIPHLDNLLGTIEKGHFCVIGGRPGSGKSTLAQMCAMQTAKRYNMPVLFISAEMDTPT
LTNRMISALGHIPYNNLHNGEIYDGMFEKLTGTIAQFRNLPIFIEEKQKPTISEIQSYARKAKRKYKALGCIIVDYLGLI
RDPSKKDRVQEVASISRDLKAMAKEFDCPVIALAQLNRGAEGHKPVASDLKDSGQIEQDADQIIMVHPILEKETNAPTGV
TELIIAKNRHGKRGSVNVQDRLDICRFVGMSFPVEERGAA

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=446, Percent_Identity=29.8206278026906, Blast_Score=189, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 49068; Mature: 48937

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDIQNISIEQSVLVALMTTSNSLEVVANDLTEEHFFAGRHKIIYRAIVELSNADMPYD
CCCCCCCCCHHHHEEEEEEECCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCH
AVFVGKHLQERNLLNDIGGEEYLIQLNSAIGSVHHLEYFVAELTKLKNHREVEGIGLAIA
HHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
GRAKDLTVSDIYLEAENLFSTSSSTLEAKQTSFDFNQALEKTLERFEKKIAQKEDKGFIG
CCCCCCEEEHEEEEHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
VQFNIPHLDNLLGTIEKGHFCVIGGRPGSGKSTLAQMCAMQTAKRYNMPVLFISAEMDTP
EEECCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
TLTNRMISALGHIPYNNLHNGEIYDGMFEKLTGTIAQFRNLPIFIEEKQKPTISEIQSYA
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
RKAKRKYKALGCIIVDYLGLIRDPSKKDRVQEVASISRDLKAMAKEFDCPVIALAQLNRG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCC
AEGHKPVASDLKDSGQIEQDADQIIMVHPILEKETNAPTGVTELIIAKNRHGKRGSVNVQ
CCCCCCCHHHHHCCCCCCCCCCCEEEEEEHHHCCCCCCCCHHHHEEECCCCCCCCCCCHH
DRLDICRFVGMSFPVEERGAA
HHHHHHHHHCCCCCHHHCCCC
>Mature Secondary Structure 
SSDIQNISIEQSVLVALMTTSNSLEVVANDLTEEHFFAGRHKIIYRAIVELSNADMPYD
CCCCCCCCHHHHEEEEEEECCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCH
AVFVGKHLQERNLLNDIGGEEYLIQLNSAIGSVHHLEYFVAELTKLKNHREVEGIGLAIA
HHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
GRAKDLTVSDIYLEAENLFSTSSSTLEAKQTSFDFNQALEKTLERFEKKIAQKEDKGFIG
CCCCCCEEEHEEEEHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
VQFNIPHLDNLLGTIEKGHFCVIGGRPGSGKSTLAQMCAMQTAKRYNMPVLFISAEMDTP
EEECCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
TLTNRMISALGHIPYNNLHNGEIYDGMFEKLTGTIAQFRNLPIFIEEKQKPTISEIQSYA
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
RKAKRKYKALGCIIVDYLGLIRDPSKKDRVQEVASISRDLKAMAKEFDCPVIALAQLNRG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCC
AEGHKPVASDLKDSGQIEQDADQIIMVHPILEKETNAPTGVTELIIAKNRHGKRGSVNVQ
CCCCCCCHHHHHCCCCCCCCCCCEEEEEEHHHCCCCCCCCHHHHEEECCCCCCCCCCCHH
DRLDICRFVGMSFPVEERGAA
HHHHHHHHHCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]