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The map label for this gene is prpF [H]

Identifier: 213157704

GI number: 213157704

Start: 3308780

End: 3309967

Strand: Reverse

Name: prpF [H]

Synonym: AB57_3183

Alternate gene names: 213157704

Gene position: 3309967-3308780 (Counterclockwise)

Preceding gene: 213157707

Following gene: 213157703

Centisome position: 81.72

GC content: 43.1

Gene sequence:

>1188_bases
ATGAGCTTCGTACCGCAAATTAAAATTCCAGCAACTTACATGCGTGGTGGAACCAGTAAAGGTGTATTCTTTAAACTTGA
TGATTTGCCGGAAAAAGCTCAAGTTGCAGGTCAAGCAAGAGATCAACTCTTGCTACGTGTCATTGGTAGCCCAGACCCTT
ATGGAAAACAAATTGATGGAATGGGTGGAGCGACCTCGAGTACCAGTAAAACCGTTATTCTCGCTAAAAGTACACAGCCA
GATCATGATGTCGACTATTTGTTTGGGCAGGTTTCAATTGATCAAGCTTTTGTAGATTGGAGTGGTAACTGCGGTAATTT
GACAGCTGCGGTTGGTTCATTTGCGATTTCAAATGGTTTGGTTGATGCCGATCGTATTCCTGAAAATGGCCTCTGTACTG
TTCGAATTTGGCAAAAAAATATTCAAAAAACTATTATTGCGCATGTACCTATCACCAATGGCCAGGTTCAAGAAACCGGA
GACTTTGAACTAGATGGGGTAACTTTCCCTGCTGCTGAAGTTCAAATTGAGTTTTTAGACCCTGCCGATGATGGTGAAGA
GGGCGGTGATATGTTCCCGACAGGGAATGTTGTTGACCAATTAGATGTTCCAGAGATTGGTAGTTTTCAAGCCACATTTA
TTAATGCTGGTATTCCAACCATTTTCTTAAATGCAGAAGATTTAGGCTACGAAGGTACTGAATTACAAGATCATATTAAT
GGTGATGCAGCAGCATTAGCACGTTTTGAAAAAATTCGTGCCTATGGTGCAGTTCAGATGGGCTTAATTAAAGATATTTC
AGAAGCAGCCGCACGTCAGCACACACCAAAAATTGCTTTTGTCTCTAAACCAAAAAATTACACAGCTTCAAGCGGTAAAA
ACGTTTCAGAGAACGATGTCGACTTACTCGTTCGAGCTTTGTCGATGGGCAAACTTCACCATGCCATGATGGGAACAGCA
GCGGTAGCAATTGGTACAGCGGCTGCGATTCCAGGCACTTTAGTAAATTTAGCGGCTGGTGGTGGAGAACGTGAAGCAGT
ACGTTTTGGACATCCATCTGGAACATTACGTGTTGGTGCCCAAGCTGAACTTACAAATGGCCAATGGGTGGTGAAAAAAG
CCATTATGAGTCGTAGTGCGCGTGTGTTAATGGAAGGTTGGGTACGCGTTCCGGGTGACAGTTTCTAA

Upstream 100 bases:

>100_bases
GCTATCTCTTGATCATAAAACGATTTAAGATGAAGCGAATGACTAAACAAGTCTGCTTGTTTTGAGATATAAAAACACAA
TAGTAAGGATAACAGTTGAT

Downstream 100 bases:

>100_bases
AAGTCATTTAAATGAAAAGCCTCTCTACCCTAGAGAGGCTTTTTACTTCTGACATCCATAACTCATCACGCACTTTTTAC
AGTGAATCGTGTATGATGTA

Product: AcnD-accessory protein PrpF

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MSFVPQIKIPATYMRGGTSKGVFFKLDDLPEKAQVAGQARDQLLLRVIGSPDPYGKQIDGMGGATSSTSKTVILAKSTQP
DHDVDYLFGQVSIDQAFVDWSGNCGNLTAAVGSFAISNGLVDADRIPENGLCTVRIWQKNIQKTIIAHVPITNGQVQETG
DFELDGVTFPAAEVQIEFLDPADDGEEGGDMFPTGNVVDQLDVPEIGSFQATFINAGIPTIFLNAEDLGYEGTELQDHIN
GDAAALARFEKIRAYGAVQMGLIKDISEAAARQHTPKIAFVSKPKNYTASSGKNVSENDVDLLVRALSMGKLHHAMMGTA
AVAIGTAAAIPGTLVNLAAGGGEREAVRFGHPSGTLRVGAQAELTNGQWVVKKAIMSRSARVLMEGWVRVPGDSF

Sequences:

>Translated_395_residues
MSFVPQIKIPATYMRGGTSKGVFFKLDDLPEKAQVAGQARDQLLLRVIGSPDPYGKQIDGMGGATSSTSKTVILAKSTQP
DHDVDYLFGQVSIDQAFVDWSGNCGNLTAAVGSFAISNGLVDADRIPENGLCTVRIWQKNIQKTIIAHVPITNGQVQETG
DFELDGVTFPAAEVQIEFLDPADDGEEGGDMFPTGNVVDQLDVPEIGSFQATFINAGIPTIFLNAEDLGYEGTELQDHIN
GDAAALARFEKIRAYGAVQMGLIKDISEAAARQHTPKIAFVSKPKNYTASSGKNVSENDVDLLVRALSMGKLHHAMMGTA
AVAIGTAAAIPGTLVNLAAGGGEREAVRFGHPSGTLRVGAQAELTNGQWVVKKAIMSRSARVLMEGWVRVPGDSF
>Mature_394_residues
SFVPQIKIPATYMRGGTSKGVFFKLDDLPEKAQVAGQARDQLLLRVIGSPDPYGKQIDGMGGATSSTSKTVILAKSTQPD
HDVDYLFGQVSIDQAFVDWSGNCGNLTAAVGSFAISNGLVDADRIPENGLCTVRIWQKNIQKTIIAHVPITNGQVQETGD
FELDGVTFPAAEVQIEFLDPADDGEEGGDMFPTGNVVDQLDVPEIGSFQATFINAGIPTIFLNAEDLGYEGTELQDHING
DAAALARFEKIRAYGAVQMGLIKDISEAAARQHTPKIAFVSKPKNYTASSGKNVSENDVDLLVRALSMGKLHHAMMGTAA
VAIGTAAAIPGTLVNLAAGGGEREAVRFGHPSGTLRVGAQAELTNGQWVVKKAIMSRSARVLMEGWVRVPGDSF

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=385, Percent_Identity=35.0649350649351, Blast_Score=181, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 41947; Mature: 41816

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFVPQIKIPATYMRGGTSKGVFFKLDDLPEKAQVAGQARDQLLLRVIGSPDPYGKQIDG
CCCCCCCCCCHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
MGGATSSTSKTVILAKSTQPDHDVDYLFGQVSIDQAFVDWSGNCGNLTAAVGSFAISNGL
CCCCCCCCCEEEEEEECCCCCCCHHHEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCC
VDADRIPENGLCTVRIWQKNIQKTIIAHVPITNGQVQETGDFELDGVTFPAAEVQIEFLD
CCHHCCCCCCEEEEEEEHHCCCEEEEEEECCCCCCCCCCCCEEECCEECCCEEEEEEEEC
PADDGEEGGDMFPTGNVVDQLDVPEIGSFQATFINAGIPTIFLNAEDLGYEGTELQDHIN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECHHHCCCCCCCHHHHCC
GDAAALARFEKIRAYGAVQMGLIKDISEAAARQHTPKIAFVSKPKNYTASSGKNVSENDV
CHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHH
DLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGEREAVRFGHPSGTLRVGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCEEECC
QAELTNGQWVVKKAIMSRSARVLMEGWVRVPGDSF
EEEECCCHHHHHHHHHHHHHHHHHHCCEECCCCCC
>Mature Secondary Structure 
SFVPQIKIPATYMRGGTSKGVFFKLDDLPEKAQVAGQARDQLLLRVIGSPDPYGKQIDG
CCCCCCCCCHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
MGGATSSTSKTVILAKSTQPDHDVDYLFGQVSIDQAFVDWSGNCGNLTAAVGSFAISNGL
CCCCCCCCCEEEEEEECCCCCCCHHHEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCC
VDADRIPENGLCTVRIWQKNIQKTIIAHVPITNGQVQETGDFELDGVTFPAAEVQIEFLD
CCHHCCCCCCEEEEEEEHHCCCEEEEEEECCCCCCCCCCCCEEECCEECCCEEEEEEEEC
PADDGEEGGDMFPTGNVVDQLDVPEIGSFQATFINAGIPTIFLNAEDLGYEGTELQDHIN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECHHHCCCCCCCHHHHCC
GDAAALARFEKIRAYGAVQMGLIKDISEAAARQHTPKIAFVSKPKNYTASSGKNVSENDV
CHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHH
DLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGEREAVRFGHPSGTLRVGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCEEECC
QAELTNGQWVVKKAIMSRSARVLMEGWVRVPGDSF
EEEECCCHHHHHHHHHHHHHHHHHHCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA