Click here to switch to the map view.

The map label for this gene is pmrC [H]

Identifier: 213157695

GI number: 213157695

Start: 3299099

End: 3300700

Strand: Reverse

Name: pmrC [H]

Synonym: AB57_3174

Alternate gene names: 213157695

Gene position: 3300700-3299099 (Counterclockwise)

Preceding gene: 213157696

Following gene: 213157694

Centisome position: 81.49

GC content: 37.02

Gene sequence:

>1602_bases
ATGTTTAATCTCATTATAGCCATTTGGCTAGGTGCAATTTTAAATATTGGTTTTTATCATCAAGTCCATACTTTAACTCC
ATATTTTGGAGTAAAGGCTATTTTGTTTTTAGCTGCTACTCTCGTAATTCTTGTTGCTACCTATTATGCGGTTTTACAAA
TCTTAAATTGGAAATGGACTGCCAAAATCTTTGCAATTTTATTGATATTTATTGGTGGCTTTAGCTCTTATTTTGTAAAC
ACATTGGGTGTCATTATTTCACCTGACCAAATTCAAAACATGGTCCAGACAGATGTTTCAGAAGTTACCGATCTAATCTC
TTTACGCTTTGTTTTATGGACAGTTTTTTTTGTTATTTTACCCATTTTTTTAATTACTCAAGTTAAATTTAAACAAGAAA
AAGTATCACGGTTGTTATTGAAGAAAGTATTCTCACTGGTAGCTTCATTTGCAGTGGTCGGTGTTTTACTTTTTACCTAC
TATGTCGATTTCGCTGCAATATTTCGTGAACATCGTGATTTAAAAGGGATGATTTCACCGCAAAATAGTATTTCATCGCT
TATGTCTTACTATCATAAGAAGGCTCCGAAGAAAAATCTGCCTCTTGTGATATATGGACAAGATGCTCATCAAGTTCAGC
GCGTACAAAAGAACCTCCCTAAGTTAATGATACTTGTTGTAGGTGAAACGGCACGTGCCGAAAGTTTCTCTCTAAATGGG
TATGCAAAAAATACGAATCCGGAGCTTTCTAAACAAGATATTTTCAACTTTTCGCAAGTGAGCTCATGCGGTACGGCGAC
AGCAGTTTCTGTGCCATGTATGTTCTCGGGTATGCCACGTGTAGATTATGATGAGCAATTAGCCAGTCACCGCGAAGGTT
TACTAGATATTGCAAAACGTGCGGGTTACCAAGTGACTTGGATTGATAATAACTCGGGTTGTAAAGGTGCATGTGATCGT
GTTGAACAATACCAGATTCCAGAAAACTTAAAGAAAAAATGGTGTAAAGATGGCGAATGTTATGATGACATTCTCATTGA
CAGCTTAAAGCAGTATTTGGCTACTATTGCCAAAGATGATGATCGCCCACGTTTGATTGTTTTGCATCAGGTGGGTAGTC
ACGGGCCTGCATATTACAAGCGTGCGCCTGAGGCATATCAACCCTTTAAACCGACTTGTGATACGAATGCGATACAGGGC
TGTTCGCAAACCGAATTGCTAAATAGTTATGATAATACAATCGTATATACAGACCATGTATTAAGCCAAATGATTAATAC
TCTAAAAGAAATATCAAAATATCAGACAGGTTTATGGTATTTATCTGATCATGGCGAATCAACCGGAGAACATGGTTTAT
ATTTACATGGTTCACCTTATGCAATCGCACCGAGCCAACAAACACATGTACCAATGATTATGTGGTTCTCTGAAAGTTGG
AAACAACGTAATCTTGCTCAAGTGAATTGTTTAAGCCAACAAACTAAACAAAAGTTAAGTCAGGATAATTTATTCCCAAG
TTTGTTAAGTTTGCTGGATGTAAAAACTCAGGTCATCAACCCTCAACTGGACATGTTGCACTCTTGTGCCCATGTAAACT
AA

Upstream 100 bases:

>100_bases
TAAGTTTCAATTTCTACATTAAAGCATCATAAAAAGATTGTAGTCACTCACGATGCTGAATTTTTTTTCAACATTAAGAA
ATAAACAGATTTCTCTGTTT

Downstream 100 bases:

>100_bases
AGCGAGCCTAGAACATGACAAAAATCTTGATGATTGAAGATGATTTTATGATTGCAGAATCAACGATCACGTTGCTGCAA
TATCATCAGTTTGAGGTGGA

Product: lipid A phosphoethanolamine transferase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MFNLIIAIWLGAILNIGFYHQVHTLTPYFGVKAILFLAATLVILVATYYAVLQILNWKWTAKIFAILLIFIGGFSSYFVN
TLGVIISPDQIQNMVQTDVSEVTDLISLRFVLWTVFFVILPIFLITQVKFKQEKVSRLLLKKVFSLVASFAVVGVLLFTY
YVDFAAIFREHRDLKGMISPQNSISSLMSYYHKKAPKKNLPLVIYGQDAHQVQRVQKNLPKLMILVVGETARAESFSLNG
YAKNTNPELSKQDIFNFSQVSSCGTATAVSVPCMFSGMPRVDYDEQLASHREGLLDIAKRAGYQVTWIDNNSGCKGACDR
VEQYQIPENLKKKWCKDGECYDDILIDSLKQYLATIAKDDDRPRLIVLHQVGSHGPAYYKRAPEAYQPFKPTCDTNAIQG
CSQTELLNSYDNTIVYTDHVLSQMINTLKEISKYQTGLWYLSDHGESTGEHGLYLHGSPYAIAPSQQTHVPMIMWFSESW
KQRNLAQVNCLSQQTKQKLSQDNLFPSLLSLLDVKTQVINPQLDMLHSCAHVN

Sequences:

>Translated_533_residues
MFNLIIAIWLGAILNIGFYHQVHTLTPYFGVKAILFLAATLVILVATYYAVLQILNWKWTAKIFAILLIFIGGFSSYFVN
TLGVIISPDQIQNMVQTDVSEVTDLISLRFVLWTVFFVILPIFLITQVKFKQEKVSRLLLKKVFSLVASFAVVGVLLFTY
YVDFAAIFREHRDLKGMISPQNSISSLMSYYHKKAPKKNLPLVIYGQDAHQVQRVQKNLPKLMILVVGETARAESFSLNG
YAKNTNPELSKQDIFNFSQVSSCGTATAVSVPCMFSGMPRVDYDEQLASHREGLLDIAKRAGYQVTWIDNNSGCKGACDR
VEQYQIPENLKKKWCKDGECYDDILIDSLKQYLATIAKDDDRPRLIVLHQVGSHGPAYYKRAPEAYQPFKPTCDTNAIQG
CSQTELLNSYDNTIVYTDHVLSQMINTLKEISKYQTGLWYLSDHGESTGEHGLYLHGSPYAIAPSQQTHVPMIMWFSESW
KQRNLAQVNCLSQQTKQKLSQDNLFPSLLSLLDVKTQVINPQLDMLHSCAHVN
>Mature_533_residues
MFNLIIAIWLGAILNIGFYHQVHTLTPYFGVKAILFLAATLVILVATYYAVLQILNWKWTAKIFAILLIFIGGFSSYFVN
TLGVIISPDQIQNMVQTDVSEVTDLISLRFVLWTVFFVILPIFLITQVKFKQEKVSRLLLKKVFSLVASFAVVGVLLFTY
YVDFAAIFREHRDLKGMISPQNSISSLMSYYHKKAPKKNLPLVIYGQDAHQVQRVQKNLPKLMILVVGETARAESFSLNG
YAKNTNPELSKQDIFNFSQVSSCGTATAVSVPCMFSGMPRVDYDEQLASHREGLLDIAKRAGYQVTWIDNNSGCKGACDR
VEQYQIPENLKKKWCKDGECYDDILIDSLKQYLATIAKDDDRPRLIVLHQVGSHGPAYYKRAPEAYQPFKPTCDTNAIQG
CSQTELLNSYDNTIVYTDHVLSQMINTLKEISKYQTGLWYLSDHGESTGEHGLYLHGSPYAIAPSQQTHVPMIMWFSESW
KQRNLAQVNCLSQQTKQKLSQDNLFPSLLSLLDVKTQVINPQLDMLHSCAHVN

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=531, Percent_Identity=42.5612052730697, Blast_Score=425, Evalue=1e-120,
Organism=Escherichia coli, GI87082286, Length=525, Percent_Identity=24.7619047619048, Blast_Score=172, Evalue=7e-44,
Organism=Escherichia coli, GI87082223, Length=466, Percent_Identity=25.5364806866953, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1790392, Length=465, Percent_Identity=23.010752688172, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 60333; Mature: 60333

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNLIIAIWLGAILNIGFYHQVHTLTPYFGVKAILFLAATLVILVATYYAVLQILNWKWT
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
AKIFAILLIFIGGFSSYFVNTLGVIISPDQIQNMVQTDVSEVTDLISLRFVLWTVFFVIL
HHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIFLITQVKFKQEKVSRLLLKKVFSLVASFAVVGVLLFTYYVDFAAIFREHRDLKGMISP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QNSISSLMSYYHKKAPKKNLPLVIYGQDAHQVQRVQKNLPKLMILVVGETARAESFSLNG
HHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHCCCEEEEEEECCCCCCCCEECCC
YAKNTNPELSKQDIFNFSQVSSCGTATAVSVPCMFSGMPRVDYDEQLASHREGLLDIAKR
EECCCCCCCCHHHHCCHHHHHCCCCHHHEECCHHHCCCCCCCHHHHHHHHHHHHHHHHHH
AGYQVTWIDNNSGCKGACDRVEQYQIPENLKKKWCKDGECYDDILIDSLKQYLATIAKDD
CCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
DRPRLIVLHQVGSHGPAYYKRAPEAYQPFKPTCDTNAIQGCSQTELLNSYDNTIVYTDHV
CCCCEEEEEECCCCCCHHHHCCCCCCCCCCCCCCCHHHCCCCHHHHHHHCCCEEEEHHHH
LSQMINTLKEISKYQTGLWYLSDHGESTGEHGLYLHGSPYAIAPSQQTHVPMIMWFSESW
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEECCCCEEECCCCCCCCCEEEEECHHH
KQRNLAQVNCLSQQTKQKLSQDNLFPSLLSLLDVKTQVINPQLDMLHSCAHVN
HHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MFNLIIAIWLGAILNIGFYHQVHTLTPYFGVKAILFLAATLVILVATYYAVLQILNWKWT
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
AKIFAILLIFIGGFSSYFVNTLGVIISPDQIQNMVQTDVSEVTDLISLRFVLWTVFFVIL
HHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIFLITQVKFKQEKVSRLLLKKVFSLVASFAVVGVLLFTYYVDFAAIFREHRDLKGMISP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QNSISSLMSYYHKKAPKKNLPLVIYGQDAHQVQRVQKNLPKLMILVVGETARAESFSLNG
HHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHCCCEEEEEEECCCCCCCCEECCC
YAKNTNPELSKQDIFNFSQVSSCGTATAVSVPCMFSGMPRVDYDEQLASHREGLLDIAKR
EECCCCCCCCHHHHCCHHHHHCCCCHHHEECCHHHCCCCCCCHHHHHHHHHHHHHHHHHH
AGYQVTWIDNNSGCKGACDRVEQYQIPENLKKKWCKDGECYDDILIDSLKQYLATIAKDD
CCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
DRPRLIVLHQVGSHGPAYYKRAPEAYQPFKPTCDTNAIQGCSQTELLNSYDNTIVYTDHV
CCCCEEEEEECCCCCCHHHHCCCCCCCCCCCCCCCHHHCCCCHHHHHHHCCCEEEEHHHH
LSQMINTLKEISKYQTGLWYLSDHGESTGEHGLYLHGSPYAIAPSQQTHVPMIMWFSESW
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEECCCCEEECCCCCCCCCEEEEECHHH
KQRNLAQVNCLSQQTKQKLSQDNLFPSLLSLLDVKTQVINPQLDMLHSCAHVN
HHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]