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The map label for this gene is dhaT [H]

Identifier: 213157663

GI number: 213157663

Start: 3264774

End: 3265958

Strand: Direct

Name: dhaT [H]

Synonym: AB57_3141

Alternate gene names: 213157663

Gene position: 3264774-3265958 (Clockwise)

Preceding gene: 213157662

Following gene: 213157664

Centisome position: 80.6

GC content: 40.93

Gene sequence:

>1185_bases
ATGGCTAAACCATATTACGAATTCTTTTGTCCTGTAAAAGTCATTGCCGGTAATGCTGCTTTAGAACATATCCCGTTTGA
ACTTGCGACTTTAGGTGCAAAATGCCCTCTTATCATTACCGATAAAGGTGTTCGTGCAAATGGCTTGTTAAACCCTATTG
AAGCTGCTTTTAGCACAACTGATGCAGTCATTGGTCATGTTTTTGATGATGTTCCACCAGACTCAAGCCTTGAAGTTGTT
CGACTTGCCGCGGAAGCTTATCGCACAAATAAATGTGATGCGATTATTGCTGTGGGTGGTGGTTCTGTTATCGATACCTC
TAAAGCAACTAACATTTTAGTATCAGAAGGTGGAGATGATCTTCTTCAATATTCTGGTGCACACAACCTACCAAAACCAT
TAAAACCATTTTTCGTCATCCCGACTACATCAGGTACGGGATCTGAAGTGACCATGGTTGCTGTTGTGTCTGATACACAA
AAGAATGTTAAATTGGCATTTGCTTCATATTACTTAATGCCACATGCTGCGATTTTAGATCCACGCATGACATTAACTTT
ACCACCTCACTTAACTGCAATGACGGGTATGGATGCGTTAACACATTGTGTTGAAGCATACACCTGTATGGCAGCCAACC
CACTCAGCGATGCCTATGCAAGTGCAGGTATTAAAAAGATTAGCGAAAATCTATTTAATGTCTTAGAAAATCCAAATGAC
TCACAAGGTCGTTTAGAACTTGCACAAGCTTCTACAATGGCAGGTATCGCATTCTCTAACTCGATGGTAGGCCTTGTACA
CTCATTAGGACATGCATTAGGTGCAGTTGCTCACCTGCCCCACGGGCTGTGCATGAACTTATTCTTACCTTATGTGCTTG
AGTACAATAAACAAGTCAATGGTAAGAAAATTGGCGAGTTGCTATTACCACTTGCAGGTGCCGATATTTATGCCCAGACT
CCTGCTACTCAGCGTGCAGACAGAGCAATCGCAACTATTTTAGCAATGCGTGATCGTTTATTTAGCTTAACCAAATTACC
ACGTACCTTACGTGAAACAGGTAAAGTTACTGAAGCACAGCTTGATGAAGTGGCAGAGAAAGCACTAAATGATGGCTCAA
TTATTTATAATCCTAAAGAAGCAAGCTTAAAAGATCTACGAGAGATCTTACAAAAAGCTTGGTAA

Upstream 100 bases:

>100_bases
AAAGAATACCCAACTGAACTTACCCTAAAAGAAAAATTCAACCAAGCTGCAAATATTTATTTAAAAGTAGCTAAGTCTTA
CTTCAAGCGGAGCGCATAAC

Downstream 100 bases:

>100_bases
GTTAAGATTTAGATATCTAAAGCAAAAAGCTAAAGGTCTGATTTTTATGACAAATCAGACCTTTTTTATATCTATGAATT
TTTATTCATCATTTTCTTTG

Product: alcohol dehydrogenase, class IV

Products: NA

Alternate protein names: 1,3-propanediol oxidoreductase; 3-hydroxypropionaldehyde reductase [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAKPYYEFFCPVKVIAGNAALEHIPFELATLGAKCPLIITDKGVRANGLLNPIEAAFSTTDAVIGHVFDDVPPDSSLEVV
RLAAEAYRTNKCDAIIAVGGGSVIDTSKATNILVSEGGDDLLQYSGAHNLPKPLKPFFVIPTTSGTGSEVTMVAVVSDTQ
KNVKLAFASYYLMPHAAILDPRMTLTLPPHLTAMTGMDALTHCVEAYTCMAANPLSDAYASAGIKKISENLFNVLENPND
SQGRLELAQASTMAGIAFSNSMVGLVHSLGHALGAVAHLPHGLCMNLFLPYVLEYNKQVNGKKIGELLLPLAGADIYAQT
PATQRADRAIATILAMRDRLFSLTKLPRTLRETGKVTEAQLDEVAEKALNDGSIIYNPKEASLKDLREILQKAW

Sequences:

>Translated_394_residues
MAKPYYEFFCPVKVIAGNAALEHIPFELATLGAKCPLIITDKGVRANGLLNPIEAAFSTTDAVIGHVFDDVPPDSSLEVV
RLAAEAYRTNKCDAIIAVGGGSVIDTSKATNILVSEGGDDLLQYSGAHNLPKPLKPFFVIPTTSGTGSEVTMVAVVSDTQ
KNVKLAFASYYLMPHAAILDPRMTLTLPPHLTAMTGMDALTHCVEAYTCMAANPLSDAYASAGIKKISENLFNVLENPND
SQGRLELAQASTMAGIAFSNSMVGLVHSLGHALGAVAHLPHGLCMNLFLPYVLEYNKQVNGKKIGELLLPLAGADIYAQT
PATQRADRAIATILAMRDRLFSLTKLPRTLRETGKVTEAQLDEVAEKALNDGSIIYNPKEASLKDLREILQKAW
>Mature_393_residues
AKPYYEFFCPVKVIAGNAALEHIPFELATLGAKCPLIITDKGVRANGLLNPIEAAFSTTDAVIGHVFDDVPPDSSLEVVR
LAAEAYRTNKCDAIIAVGGGSVIDTSKATNILVSEGGDDLLQYSGAHNLPKPLKPFFVIPTTSGTGSEVTMVAVVSDTQK
NVKLAFASYYLMPHAAILDPRMTLTLPPHLTAMTGMDALTHCVEAYTCMAANPLSDAYASAGIKKISENLFNVLENPNDS
QGRLELAQASTMAGIAFSNSMVGLVHSLGHALGAVAHLPHGLCMNLFLPYVLEYNKQVNGKKIGELLLPLAGADIYAQTP
ATQRADRAIATILAMRDRLFSLTKLPRTLRETGKVTEAQLDEVAEKALNDGSIIYNPKEASLKDLREILQKAW

Specific function: Most active with substrates containing two primary alcohol groups separated by one or two carbon atoms. In the physiological direction, 3-hydroxypropionaldehyde is the preferred substrate [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=370, Percent_Identity=30.5405405405405, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI48994951, Length=387, Percent_Identity=31.0077519379845, Blast_Score=194, Evalue=6e-51,
Organism=Escherichia coli, GI87082107, Length=299, Percent_Identity=36.4548494983278, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1789163, Length=380, Percent_Identity=31.3157894736842, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1787493, Length=388, Percent_Identity=32.9896907216495, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI1789386, Length=314, Percent_Identity=27.3885350318471, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17537053, Length=315, Percent_Identity=29.2063492063492, Blast_Score=137, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6321181, Length=389, Percent_Identity=33.1619537275064, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24657991, Length=311, Percent_Identity=30.8681672025724, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.202 [H]

Molecular weight: Translated: 42220; Mature: 42089

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPYYEFFCPVKVIAGNAALEHIPFELATLGAKCPLIITDKGVRANGLLNPIEAAFSTT
CCCCCHHHHCCCCEEECCHHHHHCCHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHH
DAVIGHVFDDVPPDSSLEVVRLAAEAYRTNKCDAIIAVGGGSVIDTSKATNILVSEGGDD
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCEEEEECCCCH
LLQYSGAHNLPKPLKPFFVIPTTSGTGSEVTMVAVVSDTQKNVKLAFASYYLMPHAAILD
HHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCHHHCC
PRMTLTLPPHLTAMTGMDALTHCVEAYTCMAANPLSDAYASAGIKKISENLFNVLENPND
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
SQGRLELAQASTMAGIAFSNSMVGLVHSLGHALGAVAHLPHGLCMNLFLPYVLEYNKQVN
CCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
GKKIGELLLPLAGADIYAQTPATQRADRAIATILAMRDRLFSLTKLPRTLRETGKVTEAQ
HHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LDEVAEKALNDGSIIYNPKEASLKDLREILQKAW
HHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AKPYYEFFCPVKVIAGNAALEHIPFELATLGAKCPLIITDKGVRANGLLNPIEAAFSTT
CCCCHHHHCCCCEEECCHHHHHCCHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHH
DAVIGHVFDDVPPDSSLEVVRLAAEAYRTNKCDAIIAVGGGSVIDTSKATNILVSEGGDD
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCEEEEECCCCH
LLQYSGAHNLPKPLKPFFVIPTTSGTGSEVTMVAVVSDTQKNVKLAFASYYLMPHAAILD
HHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCHHHCC
PRMTLTLPPHLTAMTGMDALTHCVEAYTCMAANPLSDAYASAGIKKISENLFNVLENPND
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
SQGRLELAQASTMAGIAFSNSMVGLVHSLGHALGAVAHLPHGLCMNLFLPYVLEYNKQVN
CCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
GKKIGELLLPLAGADIYAQTPATQRADRAIATILAMRDRLFSLTKLPRTLRETGKVTEAQ
HHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LDEVAEKALNDGSIIYNPKEASLKDLREILQKAW
HHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7721705 [H]