Click here to switch to the map view.

The map label for this gene is lipB [H]

Identifier: 213157661

GI number: 213157661

Start: 3263505

End: 3264158

Strand: Direct

Name: lipB [H]

Synonym: AB57_3139

Alternate gene names: 213157661

Gene position: 3263505-3264158 (Clockwise)

Preceding gene: 213157660

Following gene: 213157662

Centisome position: 80.57

GC content: 40.06

Gene sequence:

>654_bases
ATGAGCCTTGATAAACCCACTTTGATTATTCGTCAGTGGAACGATTTACAAGATTATCAAAGCAAATTCGAATCGATGAA
GAACCTGACCAATCAACGTGATGAAAATACAGCAGACGAGCTGTGGTTACTCCAGCATCACGAAGTACTAACTCAAGGTC
AGGCAGGAAAACCAGAACATATTCTGATTCCATCAAACATTCCTGTGGTACAAACTGACCGTGGTGGACAAGTGACATGG
CATGGTCCTGGTCAACTCGTCGCTTACTTTATGTTTGACCTTAATCGACTGAAATGGAATGTACGCACATTGGTCAGTTT
TGCTGAACAATTTATGATAGATGTTTTAAAGAAATATAACATTGAAGCATATGCAAAACCTGATGCACCAGGCGTTTATG
TGGATGGCAGAAAGATTGGTTCATTAGGCTTCAAAATTCGCCGTGGATGTAGTTATCACGGTTTAGCCCTAAACTTGGAC
TGTGCTCTAACTGGTTTTCAAACCATCAACCCTTGTGGTTATGCAGGGCTAGAAATGGTCCGCATACAAGATTTAGTGAC
TCCATATCCATCATTTGAACAACTTTGTCAGGACTTTATTGAGTATATTAAAGCGACTGGGTACTTTAATGACCCAGAAG
TCAAAATTGAATAA

Upstream 100 bases:

>100_bases
CGTTTAGGCAAAATACTTTAAACTCATCAAAAGCATACTCTCATAATTTAAAGTTGAGAGACTTCCCTTCTGTTAAGTAC
CATGCACAGGAAAAAGTTTA

Downstream 100 bases:

>100_bases
ATGATTTTGAGATGGAATAAAAATATATTAGAGTAATTACTCTAAATTTGCTTTTGCATAATTTTAAGAAGGGATAGCAA
GTGATTTCGACAAAAGCTGT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acylcarrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSLDKPTLIIRQWNDLQDYQSKFESMKNLTNQRDENTADELWLLQHHEVLTQGQAGKPEHILIPSNIPVVQTDRGGQVTW
HGPGQLVAYFMFDLNRLKWNVRTLVSFAEQFMIDVLKKYNIEAYAKPDAPGVYVDGRKIGSLGFKIRRGCSYHGLALNLD
CALTGFQTINPCGYAGLEMVRIQDLVTPYPSFEQLCQDFIEYIKATGYFNDPEVKIE

Sequences:

>Translated_217_residues
MSLDKPTLIIRQWNDLQDYQSKFESMKNLTNQRDENTADELWLLQHHEVLTQGQAGKPEHILIPSNIPVVQTDRGGQVTW
HGPGQLVAYFMFDLNRLKWNVRTLVSFAEQFMIDVLKKYNIEAYAKPDAPGVYVDGRKIGSLGFKIRRGCSYHGLALNLD
CALTGFQTINPCGYAGLEMVRIQDLVTPYPSFEQLCQDFIEYIKATGYFNDPEVKIE
>Mature_216_residues
SLDKPTLIIRQWNDLQDYQSKFESMKNLTNQRDENTADELWLLQHHEVLTQGQAGKPEHILIPSNIPVVQTDRGGQVTWH
GPGQLVAYFMFDLNRLKWNVRTLVSFAEQFMIDVLKKYNIEAYAKPDAPGVYVDGRKIGSLGFKIRRGCSYHGLALNLDC
ALTGFQTINPCGYAGLEMVRIQDLVTPYPSFEQLCQDFIEYIKATGYFNDPEVKIE

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=135, Percent_Identity=34.0740740740741, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI87081767, Length=206, Percent_Identity=49.5145631067961, Blast_Score=210, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6323268, Length=117, Percent_Identity=36.7521367521368, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355483, Length=106, Percent_Identity=34.9056603773585, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 24837; Mature: 24705

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDKPTLIIRQWNDLQDYQSKFESMKNLTNQRDENTADELWLLQHHEVLTQGQAGKPEH
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCE
ILIPSNIPVVQTDRGGQVTWHGPGQLVAYFMFDLNRLKWNVRTLVSFAEQFMIDVLKKYN
EEECCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IEAYAKPDAPGVYVDGRKIGSLGFKIRRGCSYHGLALNLDCALTGFQTINPCGYAGLEMV
CEEEECCCCCCEEECCCEECCCCHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCCCHHHH
RIQDLVTPYPSFEQLCQDFIEYIKATGYFNDPEVKIE
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEC
>Mature Secondary Structure 
SLDKPTLIIRQWNDLQDYQSKFESMKNLTNQRDENTADELWLLQHHEVLTQGQAGKPEH
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCE
ILIPSNIPVVQTDRGGQVTWHGPGQLVAYFMFDLNRLKWNVRTLVSFAEQFMIDVLKKYN
EEECCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IEAYAKPDAPGVYVDGRKIGSLGFKIRRGCSYHGLALNLDCALTGFQTINPCGYAGLEMV
CEEEECCCCCCEEECCCEECCCCHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCCCHHHH
RIQDLVTPYPSFEQLCQDFIEYIKATGYFNDPEVKIE
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA