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The map label for this gene is rpoD [H]

Identifier: 213157659

GI number: 213157659

Start: 3261050

End: 3262936

Strand: Direct

Name: rpoD [H]

Synonym: AB57_3137

Alternate gene names: 213157659

Gene position: 3261050-3262936 (Clockwise)

Preceding gene: 213157656

Following gene: 213157660

Centisome position: 80.51

GC content: 41.28

Gene sequence:

>1887_bases
ATGAGCGATATGCATTCCCCTACTTCTCAAGTAGCGGCTCTGATTAGCCGAGGCAAAGAACAAGGTTACTTAACTTACGC
TGAGGTTAACGATCATCTGCCGGACTCGATTACTGAAAGCGAACAGATTGAAGACATCATCCAGATGCTTCAAGACGTTG
GTATTCCTGTACACGAGCGTGCACCTGAATCTGACGATACCATGTTCGGCGAATCAGCTGATGCAACTGATGAAGTTGCT
GAAGAAGAAGCTGCTGCCGTACTTGCATCTGTTGAAAATGAGCCTGGACGTACGACAGACCCTGTACGTATGTACATGCG
TGAAATGGGTACAGTAGAACTGTTAACCCGTGAAGGTGAAATCAGCATTGCTAAACGTATTGAAGAAGGCATTCGCGATG
TCTTGAACTCAATCGCTTACTGGCCAAATGCTGTTGAAGTTGTATTAAAAGAATATAATGATTTTTTAACTGGCGAACGC
CGTCTTGCCGATATCTTATCTGGTTATTTAGACCCTGAAACTGATGAAGATATTCCTGAAGTTTTAGAAGACGTTGAAGA
GCTTGAGGAAGAAGACGAATCATCTACAAAGTCAACGAAAGAAGTTAAGCTTGATGATGACGATGAAGAAGAAGAGTCTG
AAGGTGATGATGATTCTGAAGGTGATTCAGGTCCAGACCCTGAAGTTGCAAAAGTTCGTTTTGCTGAATTAGAAGCTGCA
TGGGCTCAAACTAAAGCTGTCATCGAAAAACATGGCCGTAATAGCCCTGAAGCAAATGAAGCTCTTGAGTCTTTAGCAAC
TGTATTTATGATGTTTAAATTTACTCCACGTTTATTTGACATCATTTCAGAAATGATTCGCGGAACTCATGAACAAATTC
GTGCCAATGAACGTGAAATTATGCGTTATGCAGTACGTCGTGGCCGTATGGACCGTAACCAGTTCCGTACATCTTTCCCG
AAACAGGAATCTAATCCTGCTTGGTTAGATGAACAAATTGCTAAAGCTCCAGCTGAAATCAAGGCTCACTTGGAAAAAGT
TCGTCCTGATGTTTTAGCCTTCCAGCAAAAAATTGCAGATATCGAAAAAGAGCTTGGCCTAAACGTTAGTGAAATTAAAG
ATATTTCTAAACGTATGGCAATTGGTGAAGCCAAAGCTCGTCGTGCGAAAAAAGAAATGGTAGAGGCCAACCTTCGTTTG
GTAATTTCGATTGCGAAAAAATATACCAACCGTGGTTTACAGTTCCTTGACTTGATTCAAGAAGGGAACATTGGTTTGAT
GAAAGCCGTAGACAAGTTTGAATACCGTCGTGGTTATAAATTCTCGACTTATGCGACTTGGTGGATTCGTCAGGCAATTA
CCCGTTCTATTGCCGATCAGGCTCGTACAATTCGTATTCCTGTACACATGATTGAAACGATTAACAAAATTAACCGTGTA
TCGCGTCAATTATTACAGGAAATGGGCCGTGAACCTACTCCTGAAGAGTTAGGCGAACGCTTAGAAATGGATGAGGTTAA
AGTTCGTAAGGTTCTTAAAATTGCGAAAGAGCCAATTTCAATGGAAACTCCAATCGGTGATGATGAAGATTCGCATCTTG
GGGATTTTATTGAAGACCAGAACATCACTTCTCCAATTGATGCTGCGACTTCAGAAGGCTTAAAAGAAGCAACTCGTGAA
GTTTTAGAAAACTTAACTGAACGTGAAGCAAAAGTATTGAAGATGCGTTTTGGTATTGATATGCCAACCGATCATACTTT
AGAAGAAGTAGGTAAGCAGTTTGATGTAACACGTGAGCGTATTCGTCAGATTGAAGCGAAAGCATTGCGTAAATTACGTC
ACCCTTCTCGTTCTGAACACTTGCGTTCTTTCCTAGAGAATGACTAA

Upstream 100 bases:

>100_bases
TTTATTTAACGGTCACCGATCAAAGCCCAAACATTTCAAGCAAATTGTGCTATCATGCACGGTTTTTCACTGCCACAGAT
TCAACAAAGAGTAAGCAAAG

Downstream 100 bases:

>100_bases
TTTTTGAAATCTTGAAATTTCGAGACTAGTCCCCATTTAATAGAAAGAGCTATAAACGCCTGCGATGATGCAGGCGTTTC
ACATGATGAGGTTACTTATG

Product: RNA polymerase sigma factor sigma-70

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 628; Mature: 627

Protein sequence:

>628_residues
MSDMHSPTSQVAALISRGKEQGYLTYAEVNDHLPDSITESEQIEDIIQMLQDVGIPVHERAPESDDTMFGESADATDEVA
EEEAAAVLASVENEPGRTTDPVRMYMREMGTVELLTREGEISIAKRIEEGIRDVLNSIAYWPNAVEVVLKEYNDFLTGER
RLADILSGYLDPETDEDIPEVLEDVEELEEEDESSTKSTKEVKLDDDDEEEESEGDDDSEGDSGPDPEVAKVRFAELEAA
WAQTKAVIEKHGRNSPEANEALESLATVFMMFKFTPRLFDIISEMIRGTHEQIRANEREIMRYAVRRGRMDRNQFRTSFP
KQESNPAWLDEQIAKAPAEIKAHLEKVRPDVLAFQQKIADIEKELGLNVSEIKDISKRMAIGEAKARRAKKEMVEANLRL
VISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKINRV
SRQLLQEMGREPTPEELGERLEMDEVKVRKVLKIAKEPISMETPIGDDEDSHLGDFIEDQNITSPIDAATSEGLKEATRE
VLENLTEREAKVLKMRFGIDMPTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEHLRSFLEND

Sequences:

>Translated_628_residues
MSDMHSPTSQVAALISRGKEQGYLTYAEVNDHLPDSITESEQIEDIIQMLQDVGIPVHERAPESDDTMFGESADATDEVA
EEEAAAVLASVENEPGRTTDPVRMYMREMGTVELLTREGEISIAKRIEEGIRDVLNSIAYWPNAVEVVLKEYNDFLTGER
RLADILSGYLDPETDEDIPEVLEDVEELEEEDESSTKSTKEVKLDDDDEEEESEGDDDSEGDSGPDPEVAKVRFAELEAA
WAQTKAVIEKHGRNSPEANEALESLATVFMMFKFTPRLFDIISEMIRGTHEQIRANEREIMRYAVRRGRMDRNQFRTSFP
KQESNPAWLDEQIAKAPAEIKAHLEKVRPDVLAFQQKIADIEKELGLNVSEIKDISKRMAIGEAKARRAKKEMVEANLRL
VISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKINRV
SRQLLQEMGREPTPEELGERLEMDEVKVRKVLKIAKEPISMETPIGDDEDSHLGDFIEDQNITSPIDAATSEGLKEATRE
VLENLTEREAKVLKMRFGIDMPTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEHLRSFLEND
>Mature_627_residues
SDMHSPTSQVAALISRGKEQGYLTYAEVNDHLPDSITESEQIEDIIQMLQDVGIPVHERAPESDDTMFGESADATDEVAE
EEAAAVLASVENEPGRTTDPVRMYMREMGTVELLTREGEISIAKRIEEGIRDVLNSIAYWPNAVEVVLKEYNDFLTGERR
LADILSGYLDPETDEDIPEVLEDVEELEEEDESSTKSTKEVKLDDDDEEEESEGDDDSEGDSGPDPEVAKVRFAELEAAW
AQTKAVIEKHGRNSPEANEALESLATVFMMFKFTPRLFDIISEMIRGTHEQIRANEREIMRYAVRRGRMDRNQFRTSFPK
QESNPAWLDEQIAKAPAEIKAHLEKVRPDVLAFQQKIADIEKELGLNVSEIKDISKRMAIGEAKARRAKKEMVEANLRLV
ISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKINRVS
RQLLQEMGREPTPEELGERLEMDEVKVRKVLKIAKEPISMETPIGDDEDSHLGDFIEDQNITSPIDAATSEGLKEATREV
LENLTEREAKVLKMRFGIDMPTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEHLRSFLEND

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=624, Percent_Identity=61.8589743589744, Blast_Score=720, Evalue=0.0,
Organism=Escherichia coli, GI1789098, Length=247, Percent_Identity=40.8906882591093, Blast_Score=206, Evalue=5e-54,
Organism=Escherichia coli, GI1789871, Length=252, Percent_Identity=27.3809523809524, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=29.2079207920792, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 71678; Mature: 71547

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDMHSPTSQVAALISRGKEQGYLTYAEVNDHLPDSITESEQIEDIIQMLQDVGIPVHER
CCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
APESDDTMFGESADATDEVAEEEAAAVLASVENEPGRTTDPVRMYMREMGTVELLTREGE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHCCCC
ISIAKRIEEGIRDVLNSIAYWPNAVEVVLKEYNDFLTGERRLADILSGYLDPETDEDIPE
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHH
VLEDVEELEEEDESSTKSTKEVKLDDDDEEEESEGDDDSEGDSGPDPEVAKVRFAELEAA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
WAQTKAVIEKHGRNSPEANEALESLATVFMMFKFTPRLFDIISEMIRGTHEQIRANEREI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHH
MRYAVRRGRMDRNQFRTSFPKQESNPAWLDEQIAKAPAEIKAHLEKVRPDVLAFQQKIAD
HHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHCHHHHHHHHHHHH
IEKELGLNVSEIKDISKRMAIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQ
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKINRV
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHH
SRQLLQEMGREPTPEELGERLEMDEVKVRKVLKIAKEPISMETPIGDDEDSHLGDFIEDQ
HHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCC
NITSPIDAATSEGLKEATREVLENLTEREAKVLKMRFGIDMPTDHTLEEVGKQFDVTRER
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
IRQIEAKALRKLRHPSRSEHLRSFLEND
HHHHHHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SDMHSPTSQVAALISRGKEQGYLTYAEVNDHLPDSITESEQIEDIIQMLQDVGIPVHER
CCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
APESDDTMFGESADATDEVAEEEAAAVLASVENEPGRTTDPVRMYMREMGTVELLTREGE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHCCCC
ISIAKRIEEGIRDVLNSIAYWPNAVEVVLKEYNDFLTGERRLADILSGYLDPETDEDIPE
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHH
VLEDVEELEEEDESSTKSTKEVKLDDDDEEEESEGDDDSEGDSGPDPEVAKVRFAELEAA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
WAQTKAVIEKHGRNSPEANEALESLATVFMMFKFTPRLFDIISEMIRGTHEQIRANEREI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHH
MRYAVRRGRMDRNQFRTSFPKQESNPAWLDEQIAKAPAEIKAHLEKVRPDVLAFQQKIAD
HHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHCHHHHHHHHHHHH
IEKELGLNVSEIKDISKRMAIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQ
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKINRV
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHH
SRQLLQEMGREPTPEELGERLEMDEVKVRKVLKIAKEPISMETPIGDDEDSHLGDFIEDQ
HHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCC
NITSPIDAATSEGLKEATREVLENLTEREAKVLKMRFGIDMPTDHTLEEVGKQFDVTRER
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
IRQIEAKALRKLRHPSRSEHLRSFLEND
HHHHHHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]