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The map label for this gene is gltA

Identifier: 213157654

GI number: 213157654

Start: 3256889

End: 3258163

Strand: Direct

Name: gltA

Synonym: AB57_3132

Alternate gene names: 213157654

Gene position: 3256889-3258163 (Clockwise)

Preceding gene: 213157644

Following gene: 213157656

Centisome position: 80.41

GC content: 42.27

Gene sequence:

>1275_bases
ATGTCTGAAGCAACTGGCAAAAAAGCCGTATTACATCTTGATGGCAAAGAAATTGAATTACCAATTTACAGTGGCACATT
AGGTCCCGATGTAATCGACGTTAAAGATGTATTGGCCTCAGGTCACTTTACTTTTGATCCTGGTTTTATGGCGACAGCTT
CATGCGAGTCTAAAATCACATTTATCGATGGTGACAAAGGTATTTTATTACACCGCGGTTACCCGATTGACCAGTTAGCG
ACTCAAGCAGACTACCTTGAAACTTGTTATTTATTATTAAATGGCGAGTTACCAACTGCTGAACAAAAAGTTGAGTTCGA
TGCGAAAGTTCGTGCTCATACTATGGTTCATGATCAAGTTAGCCGTTTCTTCAATGGTTTCCGTCGTGATGCTCACCCTA
TGGCAATCATGGTTGGTGTAGTAGGCGCATTATCTGCTTTCTATCACAACAACCTTGACATTGAAGACATCAATCACCGC
GAAATTACTGCGATTCGTTTGATTGCTAAAATTCCAACGCTTGCTGCTTGGAGCTACAAATATACTGTAGGTCAGCCATT
CATCTATCCACGTAATGACTTAAATTATGCGGAAAACTTCTTACACATGATGTTTGCAACTCCTGCAGACCGTGACTACA
AAGTAAACCCTGTTCTTGCTCGTGCAATGGATCGTATCTTTACGCTTCACGCTGACCACGAACAAAACGCGTCTACTTCT
ACAGTTCGTCTTGCTGGTTCTACTGGTGCGAATCCATATGCATGTATCTCTGCTGGTATCTCTGCTCTTTGGGGTCCTGC
ACACGGTGGTGCGAACGAAGCAGTTCTTAAAATGCTTGATGAAATCGGTAGCGTTGAAAATGTTGCTGAGTTCATGGAAA
AAGTTAAACGCAAAGAAGTTAAACTTATGGGCTTCGGTCACCGCGTTTACAAAAACTTCGATCCACGTGCTAAAGTGATG
AAGCAAACTTGTGACGAAGTTCTTGAAGCATTAGGTATCAATGATCCTCAATTAGCGCTTGCTATGGAACTTGAACGTAT
TGCATTGAACGACCCGTACTTTGTTGAACGTAAACTTTACCCTAACGTAGACTTCTACTCTGGTATCATCCTTAAAGCGA
TTGGTATCCCAACAGAAATGTTTACTGTTATCTTCGCTCTTGCACGTACAGTTGGCTGGATCAGTCACTGGTTAGAAATG
CACAGCGGTCCTTACAAAATTGGTCGTCCTCGTCAGCTTTACACTGGTGAAGTGCAACGTGACATCAAGCGTTAA

Upstream 100 bases:

>100_bases
TCACTAATCTTATAGCAAATTTTGACACCGTCTGATTCGCACATGAGAAAATTAGGATTTCGAGTCAGATAATCATTCAC
CAGGACAGGAGATCTATTGA

Downstream 100 bases:

>100_bases
TATTCGAAAGAACATTAATGTAAAAAAGCTGCCTAATGGCAGCTTTTTTTATAAATAAGTTTTTAAAAGTTATTCTTCTT
CAAACATATTCAATAAGTGA

Product: citrate synthase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MSEATGKKAVLHLDGKEIELPIYSGTLGPDVIDVKDVLASGHFTFDPGFMATASCESKITFIDGDKGILLHRGYPIDQLA
TQADYLETCYLLLNGELPTAEQKVEFDAKVRAHTMVHDQVSRFFNGFRRDAHPMAIMVGVVGALSAFYHNNLDIEDINHR
EITAIRLIAKIPTLAAWSYKYTVGQPFIYPRNDLNYAENFLHMMFATPADRDYKVNPVLARAMDRIFTLHADHEQNASTS
TVRLAGSTGANPYACISAGISALWGPAHGGANEAVLKMLDEIGSVENVAEFMEKVKRKEVKLMGFGHRVYKNFDPRAKVM
KQTCDEVLEALGINDPQLALAMELERIALNDPYFVERKLYPNVDFYSGIILKAIGIPTEMFTVIFALARTVGWISHWLEM
HSGPYKIGRPRQLYTGEVQRDIKR

Sequences:

>Translated_424_residues
MSEATGKKAVLHLDGKEIELPIYSGTLGPDVIDVKDVLASGHFTFDPGFMATASCESKITFIDGDKGILLHRGYPIDQLA
TQADYLETCYLLLNGELPTAEQKVEFDAKVRAHTMVHDQVSRFFNGFRRDAHPMAIMVGVVGALSAFYHNNLDIEDINHR
EITAIRLIAKIPTLAAWSYKYTVGQPFIYPRNDLNYAENFLHMMFATPADRDYKVNPVLARAMDRIFTLHADHEQNASTS
TVRLAGSTGANPYACISAGISALWGPAHGGANEAVLKMLDEIGSVENVAEFMEKVKRKEVKLMGFGHRVYKNFDPRAKVM
KQTCDEVLEALGINDPQLALAMELERIALNDPYFVERKLYPNVDFYSGIILKAIGIPTEMFTVIFALARTVGWISHWLEM
HSGPYKIGRPRQLYTGEVQRDIKR
>Mature_423_residues
SEATGKKAVLHLDGKEIELPIYSGTLGPDVIDVKDVLASGHFTFDPGFMATASCESKITFIDGDKGILLHRGYPIDQLAT
QADYLETCYLLLNGELPTAEQKVEFDAKVRAHTMVHDQVSRFFNGFRRDAHPMAIMVGVVGALSAFYHNNLDIEDINHRE
ITAIRLIAKIPTLAAWSYKYTVGQPFIYPRNDLNYAENFLHMMFATPADRDYKVNPVLARAMDRIFTLHADHEQNASTST
VRLAGSTGANPYACISAGISALWGPAHGGANEAVLKMLDEIGSVENVAEFMEKVKRKEVKLMGFGHRVYKNFDPRAKVMK
QTCDEVLEALGINDPQLALAMELERIALNDPYFVERKLYPNVDFYSGIILKAIGIPTEMFTVIFALARTVGWISHWLEMH
SGPYKIGRPRQLYTGEVQRDIKR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family

Homologues:

Organism=Homo sapiens, GI38327625, Length=393, Percent_Identity=27.2264631043257, Blast_Score=112, Evalue=6e-25,
Organism=Escherichia coli, GI1786939, Length=419, Percent_Identity=67.7804295942721, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI1786527, Length=362, Percent_Identity=31.767955801105, Blast_Score=159, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17555174, Length=400, Percent_Identity=27.5, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319850, Length=360, Percent_Identity=25.8333333333333, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324328, Length=357, Percent_Identity=26.0504201680672, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6325257, Length=359, Percent_Identity=26.7409470752089, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24640124, Length=400, Percent_Identity=27.25, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24640126, Length=400, Percent_Identity=27.25, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI21356863, Length=402, Percent_Identity=26.1194029850746, Blast_Score=101, Evalue=9e-22,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CISY_ACIAN (P20902)

Other databases:

- EMBL:   M33037
- PIR:   I39506
- ProteinModelPortal:   P20902
- SMR:   P20902
- BRENDA:   2.3.3.1
- GO:   GO:0005737
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953
- Gene3D:   G3DSA:1.10.580.10
- Gene3D:   G3DSA:1.10.230.10
- PANTHER:   PTHR11739
- PRINTS:   PR00143
- TIGRFAMs:   TIGR01798

Pfam domain/function: PF00285 Citrate_synt; SSF48256 Citrate_synthase_core

EC number: =2.3.3.1

Molecular weight: Translated: 47461; Mature: 47330

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: ACT_SITE 307-307 ACT_SITE 364-364

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEATGKKAVLHLDGKEIELPIYSGTLGPDVIDVKDVLASGHFTFDPGFMATASCESKIT
CCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCHHHHHHCCCEEECCCCEEECCCCCEEE
FIDGDKGILLHRGYPIDQLATQADYLETCYLLLNGELPTAEQKVEFDAKVRAHTMVHDQV
EEECCCCEEEECCCCHHHHHHHHHHHHHHHHEECCCCCCCHHHHCCCHHHHHHHHHHHHH
SRFFNGFRRDAHPMAIMVGVVGALSAFYHNNLDIEDINHREITAIRLIAKIPTLAAWSYK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHEEEEE
YTVGQPFIYPRNDLNYAENFLHMMFATPADRDYKVNPVLARAMDRIFTLHADHEQNASTS
EECCCCEEECCCCCHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHEEEECCCCCCCCCC
TVRLAGSTGANPYACISAGISALWGPAHGGANEAVLKMLDEIGSVENVAEFMEKVKRKEV
EEEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KLMGFGHRVYKNFDPRAKVMKQTCDEVLEALGINDPQLALAMELERIALNDPYFVERKLY
HHEECCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECC
PNVDFYSGIILKAIGIPTEMFTVIFALARTVGWISHWLEMHSGPYKIGRPRQLYTGEVQR
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCHHHH
DIKR
HHCC
>Mature Secondary Structure 
SEATGKKAVLHLDGKEIELPIYSGTLGPDVIDVKDVLASGHFTFDPGFMATASCESKIT
CCCCCCCEEEEECCCEEEEEEECCCCCCCCCCHHHHHHCCCEEECCCCEEECCCCCEEE
FIDGDKGILLHRGYPIDQLATQADYLETCYLLLNGELPTAEQKVEFDAKVRAHTMVHDQV
EEECCCCEEEECCCCHHHHHHHHHHHHHHHHEECCCCCCCHHHHCCCHHHHHHHHHHHHH
SRFFNGFRRDAHPMAIMVGVVGALSAFYHNNLDIEDINHREITAIRLIAKIPTLAAWSYK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHEEEEE
YTVGQPFIYPRNDLNYAENFLHMMFATPADRDYKVNPVLARAMDRIFTLHADHEQNASTS
EECCCCEEECCCCCHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHEEEECCCCCCCCCC
TVRLAGSTGANPYACISAGISALWGPAHGGANEAVLKMLDEIGSVENVAEFMEKVKRKEV
EEEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KLMGFGHRVYKNFDPRAKVMKQTCDEVLEALGINDPQLALAMELERIALNDPYFVERKLY
HHEECCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECC
PNVDFYSGIILKAIGIPTEMFTVIFALARTVGWISHWLEMHSGPYKIGRPRQLYTGEVQR
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCHHHH
DIKR
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3442626