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The map label for this gene is ilvA [H]

Identifier: 213157560

GI number: 213157560

Start: 2336187

End: 2337728

Strand: Direct

Name: ilvA [H]

Synonym: AB57_2252

Alternate gene names: 213157560

Gene position: 2336187-2337728 (Clockwise)

Preceding gene: 213157558

Following gene: 213157561

Centisome position: 57.68

GC content: 41.89

Gene sequence:

>1542_bases
ATGCTGTCTCGTGTGGTACGACAAATTTTACAAGCAACGGTTTATGATGTTGCAATTGAAACACCTCTCGAAGCAGCTCC
ACGAATTAGTCAAAAATTAAACAACACAATTCGTTTTAAGCGTGAAGATTTACAACCGGTTTTTTCTTTTAAATTACGCG
GTGCCTATAACCGTATTAGCCAATTACCGAAAGAACAACTTGAACGTGGCGTCATTTGCGCATCTGCAGGCAACCATGCG
CAAGGTGTAGCGTTGTCTGGTCAACGTTTAGGTATTCCTGCAATTATTGTTATGCCAAGTACTACCCCTGACATTAAAGT
TCAGGCAGTGAAGCGTTTGGGTGGCCAAGTTGTATTGCATGGCGATAGTTTTGATATTGCAAACAAATACGCTCAGCAAC
GTGCCGAAGCTGAAGGATTAGTTTTTATTCCACCTTATGACGACGAGTTGGTTATTGCGGGCCAAGGCACAATTGCGAAT
GAAATTTTACGCCAATGGCGTGATGTAGAATATGTTTTTGTTGCTGTTGGTGGTGGTGGCTTAATTGCTGGTGTAGCAGC
TTACTTAGGTGATGTTGCACCGCATGTCAAAGTCATCGGTGTTGAATATGAAGAGTCAGCTTGTTTAAAAGCAGCGCTAG
AAGCAAATGAACGTGTAGTCCTACCCCACGTAGGCTTATTTGCTGATGGTACAGCAGTTGCTCAAATTGGTGCTTTACCT
TTTGATATCGTTCGTCTACGCAAATCAGATAACTCTGGACCAATTGTTGAACCAGATATTGTTACTGTAAATACAGATGA
GATCTGCGCTGCAATTAAAGATACTTTTGATGAAAACCGCAGCATTGTTGAACCGTCTGGTGCAATGGCCCTAGCTGGTA
TCAAAAAATATGTTGCCGAGCATAAAATTAGCGGCAAAAACATGGTCTCGATTGTTTGCGGCGCCAACATGAACTTTGAC
CGCTTACGTTACATTGCAGAGCGCACCGAATTGGGTGAACAACGTGAAGCAATTTATGCAGTTACTCTTTCTGAAGAAAA
AGGCGCATTCTTAGGCTTCTGCCGTGCTCTACAGGGCCGTAACATTACCGAGTTTAACTACCGTGCAAATAACACAGAAG
AAGCACAAGTTTTTGTGGGTATTAGCTTAAAAGGTGGTGACACTGAACGTCATGAAATTTTAGAACAACTTAAACAGCAA
AATTATGTTGTAGATGATTTATCTGATGATGAAGTTGCTAAATTACACATTCGTTACTTGATTGGTGGTCATGCCAATCT
GGATGACGAAAGATTGTTCCGTGTTGAGTTTCCGGAACGTCCGGGTGCCTTACTCACCTTCTTAACTCGTTTAGGTCCAA
CCCACAATATTACGCTGTTCCATTATCGTAACCACGGCGCTGCTGAAGGTCGTGTACTAGTTGGCTTACAAGCAACAGAT
GCTAAACAAAACCCAGATGGTTTAATTGAAACTCTTGAGCAAATTAGCTATCCATATGCAGAAATTACTGACAATGTGGG
CTATAAACGTTTCTTGAAGTAA

Upstream 100 bases:

>100_bases
CTCAACTATTTTTTGACATTTATGCGCCAATAAACGGCGCAAATGCACAAGGGTTTGCTACAATAATTTCCTATTTTAAA
CTGTTATAGGGAAGATGAAC

Downstream 100 bases:

>100_bases
CCTTTCATTTTGATAAAAAAGCCGGCAAATATGTCGGCTTTTTTATCATTTAAGCTTATTATTTTTTATATTTACATTGC
TTCTCTTTATCTCAAAGAAA

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MLSRVVRQILQATVYDVAIETPLEAAPRISQKLNNTIRFKREDLQPVFSFKLRGAYNRISQLPKEQLERGVICASAGNHA
QGVALSGQRLGIPAIIVMPSTTPDIKVQAVKRLGGQVVLHGDSFDIANKYAQQRAEAEGLVFIPPYDDELVIAGQGTIAN
EILRQWRDVEYVFVAVGGGGLIAGVAAYLGDVAPHVKVIGVEYEESACLKAALEANERVVLPHVGLFADGTAVAQIGALP
FDIVRLRKSDNSGPIVEPDIVTVNTDEICAAIKDTFDENRSIVEPSGAMALAGIKKYVAEHKISGKNMVSIVCGANMNFD
RLRYIAERTELGEQREAIYAVTLSEEKGAFLGFCRALQGRNITEFNYRANNTEEAQVFVGISLKGGDTERHEILEQLKQQ
NYVVDDLSDDEVAKLHIRYLIGGHANLDDERLFRVEFPERPGALLTFLTRLGPTHNITLFHYRNHGAAEGRVLVGLQATD
AKQNPDGLIETLEQISYPYAEITDNVGYKRFLK

Sequences:

>Translated_513_residues
MLSRVVRQILQATVYDVAIETPLEAAPRISQKLNNTIRFKREDLQPVFSFKLRGAYNRISQLPKEQLERGVICASAGNHA
QGVALSGQRLGIPAIIVMPSTTPDIKVQAVKRLGGQVVLHGDSFDIANKYAQQRAEAEGLVFIPPYDDELVIAGQGTIAN
EILRQWRDVEYVFVAVGGGGLIAGVAAYLGDVAPHVKVIGVEYEESACLKAALEANERVVLPHVGLFADGTAVAQIGALP
FDIVRLRKSDNSGPIVEPDIVTVNTDEICAAIKDTFDENRSIVEPSGAMALAGIKKYVAEHKISGKNMVSIVCGANMNFD
RLRYIAERTELGEQREAIYAVTLSEEKGAFLGFCRALQGRNITEFNYRANNTEEAQVFVGISLKGGDTERHEILEQLKQQ
NYVVDDLSDDEVAKLHIRYLIGGHANLDDERLFRVEFPERPGALLTFLTRLGPTHNITLFHYRNHGAAEGRVLVGLQATD
AKQNPDGLIETLEQISYPYAEITDNVGYKRFLK
>Mature_513_residues
MLSRVVRQILQATVYDVAIETPLEAAPRISQKLNNTIRFKREDLQPVFSFKLRGAYNRISQLPKEQLERGVICASAGNHA
QGVALSGQRLGIPAIIVMPSTTPDIKVQAVKRLGGQVVLHGDSFDIANKYAQQRAEAEGLVFIPPYDDELVIAGQGTIAN
EILRQWRDVEYVFVAVGGGGLIAGVAAYLGDVAPHVKVIGVEYEESACLKAALEANERVVLPHVGLFADGTAVAQIGALP
FDIVRLRKSDNSGPIVEPDIVTVNTDEICAAIKDTFDENRSIVEPSGAMALAGIKKYVAEHKISGKNMVSIVCGANMNFD
RLRYIAERTELGEQREAIYAVTLSEEKGAFLGFCRALQGRNITEFNYRANNTEEAQVFVGISLKGGDTERHEILEQLKQQ
NYVVDDLSDDEVAKLHIRYLIGGHANLDDERLFRVEFPERPGALLTFLTRLGPTHNITLFHYRNHGAAEGRVLVGLQATD
AKQNPDGLIETLEQISYPYAEITDNVGYKRFLK

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia. Deaminates L-threonine, but also L-serine to a lesser extent [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=304, Percent_Identity=31.25, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI19923959, Length=325, Percent_Identity=29.5384615384615, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI33469958, Length=324, Percent_Identity=30.5555555555556, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1790207, Length=509, Percent_Identity=52.0628683693517, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1789505, Length=320, Percent_Identity=37.8125, Blast_Score=169, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI71991565, Length=309, Percent_Identity=35.9223300970874, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17508781, Length=295, Percent_Identity=29.1525423728814, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17537387, Length=397, Percent_Identity=30.2267002518892, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320930, Length=523, Percent_Identity=48.1835564053537, Blast_Score=441, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6322631, Length=302, Percent_Identity=32.7814569536424, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319788, Length=309, Percent_Identity=25.2427184466019, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21355833, Length=319, Percent_Identity=33.5423197492163, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24645328, Length=260, Percent_Identity=30.7692307692308, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 56451; Mature: 56451

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRVVRQILQATVYDVAIETPLEAAPRISQKLNNTIRFKREDLQPVFSFKLRGAYNRIS
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHEEEEHHHCCHHHHHHHCHHHHHHH
QLPKEQLERGVICASAGNHAQGVALSGQRLGIPAIIVMPSTTPDIKVQAVKRLGGQVVLH
HCCHHHHHCCEEEECCCCCCCCEEECCCCCCCCEEEEECCCCCCHHHHHHHHCCCEEEEE
GDSFDIANKYAQQRAEAEGLVFIPPYDDELVIAGQGTIANEILRQWRDVEYVFVAVGGGG
CCCCHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCHHHHHHHHHHCCCEEEEEEECCCH
LIAGVAAYLGDVAPHVKVIGVEYEESACLKAALEANERVVLPHVGLFADGTAVAQIGALP
HHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHCCCCEEEECCCCEEECCHHHHHHCCCC
FDIVRLRKSDNSGPIVEPDIVTVNTDEICAAIKDTFDENRSIVEPSGAMALAGIKKYVAE
HHHHHEECCCCCCCEECCCEEEECHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHH
HKISGKNMVSIVCGANMNFDRLRYIAERTELGEQREAIYAVTLSEEKGAFLGFCRALQGR
HCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCCC
NITEFNYRANNTEEAQVFVGISLKGGDTERHEILEQLKQQNYVVDDLSDDEVAKLHIRYL
CCEEEECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCEECCCCCCCCCEEEEEEE
IGGHANLDDERLFRVEFPERPGALLTFLTRLGPTHNITLFHYRNHGAAEGRVLVGLQATD
ECCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEEEECC
AKQNPDGLIETLEQISYPYAEITDNVGYKRFLK
CCCCCCHHHHHHHHHCCCHHHHHCCCCHHHHCC
>Mature Secondary Structure
MLSRVVRQILQATVYDVAIETPLEAAPRISQKLNNTIRFKREDLQPVFSFKLRGAYNRIS
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHEEEEHHHCCHHHHHHHCHHHHHHH
QLPKEQLERGVICASAGNHAQGVALSGQRLGIPAIIVMPSTTPDIKVQAVKRLGGQVVLH
HCCHHHHHCCEEEECCCCCCCCEEECCCCCCCCEEEEECCCCCCHHHHHHHHCCCEEEEE
GDSFDIANKYAQQRAEAEGLVFIPPYDDELVIAGQGTIANEILRQWRDVEYVFVAVGGGG
CCCCHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCHHHHHHHHHHCCCEEEEEEECCCH
LIAGVAAYLGDVAPHVKVIGVEYEESACLKAALEANERVVLPHVGLFADGTAVAQIGALP
HHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHCCCCEEEECCCCEEECCHHHHHHCCCC
FDIVRLRKSDNSGPIVEPDIVTVNTDEICAAIKDTFDENRSIVEPSGAMALAGIKKYVAE
HHHHHEECCCCCCCEECCCEEEECHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHH
HKISGKNMVSIVCGANMNFDRLRYIAERTELGEQREAIYAVTLSEEKGAFLGFCRALQGR
HCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCCC
NITEFNYRANNTEEAQVFVGISLKGGDTERHEILEQLKQQNYVVDDLSDDEVAKLHIRYL
CCEEEECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCEECCCCCCCCCEEEEEEE
IGGHANLDDERLFRVEFPERPGALLTFLTRLGPTHNITLFHYRNHGAAEGRVLVGLQATD
ECCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEEEECC
AKQNPDGLIETLEQISYPYAEITDNVGYKRFLK
CCCCCCHHHHHHHHHCCCHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3315862; 3550695; 1379743; 9278503; 3003115; 2473940; 9562556 [H]