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The map label for this gene is pyrG [H]

Identifier: 213157543

GI number: 213157543

Start: 2317058

End: 2318695

Strand: Reverse

Name: pyrG [H]

Synonym: AB57_2232

Alternate gene names: 213157543

Gene position: 2318695-2317058 (Counterclockwise)

Preceding gene: 213157545

Following gene: 213157542

Centisome position: 57.24

GC content: 39.99

Gene sequence:

>1638_bases
ATGACCCATTTTATTTTTGTCACTGGTGGTGTGGTTTCATCGCTAGGTAAAGGTATTTCTGCTGCTTCTGTTGCTGCACT
TTTGGAAGCTCGTGGCTTAAAAGTCACAATGGTTAAAATGGATCCTTATATTAATGTCGATCCAGGGACAATGAGCCCAT
TCCAGCATGGTGAAGTTTTTGTTACCGAAGATGGTGCAGAAACAGATCTGGATCTGGGTTATTACGAACGTTTCTTACGT
CGCGCGAAAATGACCAAACTAAACAACTTCACTAGTGGTCGTGTATATCAAGACGTTTTAAATAAAGAGCGTCGTGGTGA
TTACTTAGGTGGTACAGTTCAGGTTATTCCTCATATTACCGACAATATTAAAGAACGTGTACTCCGCGCAGGCGAAGGTT
ATGATGTTGCTATCGTAGAGATCGGTGGTACCGTAGGTGATATCGAATCTTTACCGTTTATGGAGTCGGTTCGTCAGTTA
ATGGTTGAGCTGGGCCATAAACGTACCATGCTTATGCATTTAACTTTGTTGCCATATATTAAGTCAGCAGCGGAGTTAAA
AACCAAACCAACTCAGCACTCTGTAAAAGAGTTACTTTCAATCGGTATTCAACCAGATATTCTTATCTGTCGTACTGAGT
ATGATGTTGATGCAGACACTAAACGTAAGATCGCGTTATTCACTAACGTAGAGGCTCGTGCGGTTGTCGTGTGTAAAGAT
GCGAAAACGATCTATCAAATTCCACGTGGCTTCTATGAGCAAAATGTAGATGACCTGATTTGTGAACGTTTTGGTTTTAC
TGATTTACCGGAAGCTGACTTAACTGATTGGGATAATGTTGTAGAAGCATTACTTAACCCAGAATACACAGTACGTGTAG
CAATGGTCGGTAAGTATGTTGAATTACCGGATGCTTATAAATCTGTAAACGAAGCATTGTTACATGCTGGTATTAAAAAT
CGCGTAAAAGTACAAATTGATTATGTCAATGCTGAAGAGCTTGAAAGTCAAGATGTTTCTATTTTGAAAACAGCTGATGC
GATTTTAGTTCCTGGTGGTTTTGGTGAGCGTGGTACTGAAGGCAAAATGAAAGCTATTCAATACGCACGTGAAAATGGTA
TTCCTTTCTTGGGAATCTGTTTGGGTATGCAGTTGGCTGTTATTGAATATGCTCGCCATGTTGCAGGTATGCCAGAAGCT
TCTTCTACAGAATTTAACCGTTCTACAAAATATCCATTGATTGGTTTAATTACCGAATGGTTAGATGAGCGCGGTGAATT
ACAACAGCGTAGCCTTGAGTCTGACTTAGGCGGAACAATGCGTTTAGGTGCACAAAAATCAGAATTGGTTGAAGGCACAA
AAACTCGTGAAGTTTACGGAAAAGCTGAGATTACCGAACGTCATCGTCACCGTTATGAAATGAATAATCGTTTCATTGAA
GCGATTGAACAAGCGGGCATGAAGATTTCTGGTTATTCTTCAGCGCAACATTTAGTTGAAACTGTAGAAATTCCTGAACA
TCCTTGGTTTATTGCTGTACAATTCCACCCGGAATTTACAAGTTCACCACGTGATGGACATCCATTATTTGCTAGTTTTA
TTGACGCTGCAAAAACACAGCATCAAAAAAGTAAGTAA

Upstream 100 bases:

>100_bases
CTTTGAATTGATGCGGTTTGACTTTGCTTTATAGGGGCAGAGTACAGTAAAATTGCCAAACATTGTAGGCCGATTTCGGC
TTGATTGTACGAGAAACTTA

Downstream 100 bases:

>100_bases
TGCGATTTTAAAATAAACTGGGAAAGTTTAAATGTCACAATTAAAACCACAAGAAGTTGTACGTTTAGGCGATATACAAA
TGGCAAATCATTTGCCATTT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MTHFIFVTGGVVSSLGKGISAASVAALLEARGLKVTMVKMDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGYYERFLR
RAKMTKLNNFTSGRVYQDVLNKERRGDYLGGTVQVIPHITDNIKERVLRAGEGYDVAIVEIGGTVGDIESLPFMESVRQL
MVELGHKRTMLMHLTLLPYIKSAAELKTKPTQHSVKELLSIGIQPDILICRTEYDVDADTKRKIALFTNVEARAVVVCKD
AKTIYQIPRGFYEQNVDDLICERFGFTDLPEADLTDWDNVVEALLNPEYTVRVAMVGKYVELPDAYKSVNEALLHAGIKN
RVKVQIDYVNAEELESQDVSILKTADAILVPGGFGERGTEGKMKAIQYARENGIPFLGICLGMQLAVIEYARHVAGMPEA
SSTEFNRSTKYPLIGLITEWLDERGELQQRSLESDLGGTMRLGAQKSELVEGTKTREVYGKAEITERHRHRYEMNNRFIE
AIEQAGMKISGYSSAQHLVETVEIPEHPWFIAVQFHPEFTSSPRDGHPLFASFIDAAKTQHQKSK

Sequences:

>Translated_545_residues
MTHFIFVTGGVVSSLGKGISAASVAALLEARGLKVTMVKMDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGYYERFLR
RAKMTKLNNFTSGRVYQDVLNKERRGDYLGGTVQVIPHITDNIKERVLRAGEGYDVAIVEIGGTVGDIESLPFMESVRQL
MVELGHKRTMLMHLTLLPYIKSAAELKTKPTQHSVKELLSIGIQPDILICRTEYDVDADTKRKIALFTNVEARAVVVCKD
AKTIYQIPRGFYEQNVDDLICERFGFTDLPEADLTDWDNVVEALLNPEYTVRVAMVGKYVELPDAYKSVNEALLHAGIKN
RVKVQIDYVNAEELESQDVSILKTADAILVPGGFGERGTEGKMKAIQYARENGIPFLGICLGMQLAVIEYARHVAGMPEA
SSTEFNRSTKYPLIGLITEWLDERGELQQRSLESDLGGTMRLGAQKSELVEGTKTREVYGKAEITERHRHRYEMNNRFIE
AIEQAGMKISGYSSAQHLVETVEIPEHPWFIAVQFHPEFTSSPRDGHPLFASFIDAAKTQHQKSK
>Mature_544_residues
THFIFVTGGVVSSLGKGISAASVAALLEARGLKVTMVKMDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGYYERFLRR
AKMTKLNNFTSGRVYQDVLNKERRGDYLGGTVQVIPHITDNIKERVLRAGEGYDVAIVEIGGTVGDIESLPFMESVRQLM
VELGHKRTMLMHLTLLPYIKSAAELKTKPTQHSVKELLSIGIQPDILICRTEYDVDADTKRKIALFTNVEARAVVVCKDA
KTIYQIPRGFYEQNVDDLICERFGFTDLPEADLTDWDNVVEALLNPEYTVRVAMVGKYVELPDAYKSVNEALLHAGIKNR
VKVQIDYVNAEELESQDVSILKTADAILVPGGFGERGTEGKMKAIQYARENGIPFLGICLGMQLAVIEYARHVAGMPEAS
STEFNRSTKYPLIGLITEWLDERGELQQRSLESDLGGTMRLGAQKSELVEGTKTREVYGKAEITERHRHRYEMNNRFIEA
IEQAGMKISGYSSAQHLVETVEIPEHPWFIAVQFHPEFTSSPRDGHPLFASFIDAAKTQHQKSK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=557, Percent_Identity=42.9084380610413, Blast_Score=457, Evalue=1e-129,
Organism=Homo sapiens, GI28559085, Length=560, Percent_Identity=41.4285714285714, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI28559083, Length=560, Percent_Identity=41.4285714285714, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI221316689, Length=560, Percent_Identity=41.4285714285714, Blast_Score=440, Evalue=1e-123,
Organism=Escherichia coli, GI1789142, Length=544, Percent_Identity=63.6029411764706, Blast_Score=717, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=613, Percent_Identity=37.1941272430669, Blast_Score=398, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6322563, Length=566, Percent_Identity=40.4593639575972, Blast_Score=434, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6319432, Length=569, Percent_Identity=40.597539543058, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24664469, Length=557, Percent_Identity=40.754039497307, Blast_Score=423, Evalue=1e-118,
Organism=Drosophila melanogaster, GI21357815, Length=503, Percent_Identity=38.9662027833002, Blast_Score=353, Evalue=2e-97,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60956; Mature: 60825

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHFIFVTGGVVSSLGKGISAASVAALLEARGLKVTMVKMDPYINVDPGTMSPFQHGEVF
CCEEEEEECHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTEDGAETDLDLGYYERFLRRAKMTKLNNFTSGRVYQDVLNKERRGDYLGGTVQVIPHIT
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEECHHH
DNIKERVLRAGEGYDVAIVEIGGTVGDIESLPFMESVRQLMVELGHKRTMLMHLTLLPYI
HHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KSAAELKTKPTQHSVKELLSIGIQPDILICRTEYDVDADTKRKIALFTNVEARAVVVCKD
HHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECC
AKTIYQIPRGFYEQNVDDLICERFGFTDLPEADLTDWDNVVEALLNPEYTVRVAMVGKYV
CHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEECCHH
ELPDAYKSVNEALLHAGIKNRVKVQIDYVNAEELESQDVSILKTADAILVPGGFGERGTE
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHCCCCCCEEEECCEEEECCCCCCCCCC
GKMKAIQYARENGIPFLGICLGMQLAVIEYARHVAGMPEASSTEFNRSTKYPLIGLITEW
CHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
LDERGELQQRSLESDLGGTMRLGAQKSELVEGTKTREVYGKAEITERHRHRYEMNNRFIE
HHHHHHHHHHHHHHHCCCCEECCCCHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
AIEQAGMKISGYSSAQHLVETVEIPEHPWFIAVQFHPEFTSSPRDGHPLFASFIDAAKTQ
HHHHCCCEEECCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
HQKSK
HHCCC
>Mature Secondary Structure 
THFIFVTGGVVSSLGKGISAASVAALLEARGLKVTMVKMDPYINVDPGTMSPFQHGEVF
CEEEEEECHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTEDGAETDLDLGYYERFLRRAKMTKLNNFTSGRVYQDVLNKERRGDYLGGTVQVIPHIT
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEECHHH
DNIKERVLRAGEGYDVAIVEIGGTVGDIESLPFMESVRQLMVELGHKRTMLMHLTLLPYI
HHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KSAAELKTKPTQHSVKELLSIGIQPDILICRTEYDVDADTKRKIALFTNVEARAVVVCKD
HHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECC
AKTIYQIPRGFYEQNVDDLICERFGFTDLPEADLTDWDNVVEALLNPEYTVRVAMVGKYV
CHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEECCHH
ELPDAYKSVNEALLHAGIKNRVKVQIDYVNAEELESQDVSILKTADAILVPGGFGERGTE
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHCCCCCCEEEECCEEEECCCCCCCCCC
GKMKAIQYARENGIPFLGICLGMQLAVIEYARHVAGMPEASSTEFNRSTKYPLIGLITEW
CHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
LDERGELQQRSLESDLGGTMRLGAQKSELVEGTKTREVYGKAEITERHRHRYEMNNRFIE
HHHHHHHHHHHHHHHCCCCEECCCCHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
AIEQAGMKISGYSSAQHLVETVEIPEHPWFIAVQFHPEFTSSPRDGHPLFASFIDAAKTQ
HHHHCCCEEECCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
HQKSK
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA