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The map label for this gene is holB [H]

Identifier: 213157120

GI number: 213157120

Start: 1889948

End: 1890883

Strand: Direct

Name: holB [H]

Synonym: AB57_1803

Alternate gene names: 213157120

Gene position: 1889948-1890883 (Clockwise)

Preceding gene: 213157119

Following gene: 213157121

Centisome position: 46.66

GC content: 34.4

Gene sequence:

>936_bases
ATGTGGGAAACGTTAACAACACGTTTTCCTAATATTGGGCATGGCTTATTATTTTATGGTAAACAAGGTTGTGGTAAACA
TGCTTTTGCTAAGCATTTCTTAGCGTGGGTACTTTGTTTAAATAAACAACCTCAAGGTGCTTGTGGTGAATGTAGTAGCT
GTCAATGGTTAAAATCAGATACGCATCCAAACTATGTTCATATCACGACAGATGAAGAAAATAAAAAACAGAATGCAAAA
ATTAAAATAGAAAAAATTCGAGATTTATTGCCATTTGTACAACAAACTGGTGAGGGATGGCGTGTTGTTGTTATTGAGCC
AGCTGAAGCTTTAAATCTTGCGTCATCAAATGCGCTGCTAAAAACCTTGGAAGAGCCAGGTGAACGCGTCGTTTTAATCT
TGTTGGCCGATCATTATTTGAAATTACCGGCAACCATTCGAAGTCGCTTACAGCATTTTGCTTTAGACCGTATTTCATAT
ACAGAAGCCACATCTTATCTAAATGAACATTTAGCGGAAACAGCATCTGTACCACCTGAATTATTGCTAGGCCTTTCTAA
TGATATGCCTTTGCAAGCAGTTGAAATTGCGACTAGTGATTGGTTTGCTAAAAGACAGTCTTTTTTAGAAGATTGGACTA
AAGTTGTAAATCAAAAAAATATGCCTTTATTTTATTCGGCTAAATGGCAAAAAGAACTCAACTTTAGTGATTTTATGATG
CTTCTTGAATACTTGTTAGGGGATTTAATTTGTGTCAAGCTCAATCAACCACAGAAAAATATAGATTTACAGTTCGATCA
ATTGGCGCAAAACTATAATTTAGAAAGTTTATTCAGTCTTTATGAGGAATTACAGCAATCTAAAAGATTGGTTGAACAGA
ATGTACAAACACAACTCATCATAGATCAACTTTTTATAAAATTAATGAACGTTTAA

Upstream 100 bases:

>100_bases
ATCTTGATCGTTTAAATAATATGCCTGTTGAGTCTTTTGAGTAAGCGTTAATTTAATGAATCAGAATGTGACTTCTAAGG
TTTATCCGTGGCAACAGACA

Downstream 100 bases:

>100_bases
GGGGAAAACTAATGCAACCACAAATGGGTGGAATTATTCAGGTCAATATTCCAGATAGAGCAACATTACAAGCAAGTTAT
ATGGGATATGTACAAGGTGG

Product: DNA polymerase III delta prime subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MWETLTTRFPNIGHGLLFYGKQGCGKHAFAKHFLAWVLCLNKQPQGACGECSSCQWLKSDTHPNYVHITTDEENKKQNAK
IKIEKIRDLLPFVQQTGEGWRVVVIEPAEALNLASSNALLKTLEEPGERVVLILLADHYLKLPATIRSRLQHFALDRISY
TEATSYLNEHLAETASVPPELLLGLSNDMPLQAVEIATSDWFAKRQSFLEDWTKVVNQKNMPLFYSAKWQKELNFSDFMM
LLEYLLGDLICVKLNQPQKNIDLQFDQLAQNYNLESLFSLYEELQQSKRLVEQNVQTQLIIDQLFIKLMNV

Sequences:

>Translated_311_residues
MWETLTTRFPNIGHGLLFYGKQGCGKHAFAKHFLAWVLCLNKQPQGACGECSSCQWLKSDTHPNYVHITTDEENKKQNAK
IKIEKIRDLLPFVQQTGEGWRVVVIEPAEALNLASSNALLKTLEEPGERVVLILLADHYLKLPATIRSRLQHFALDRISY
TEATSYLNEHLAETASVPPELLLGLSNDMPLQAVEIATSDWFAKRQSFLEDWTKVVNQKNMPLFYSAKWQKELNFSDFMM
LLEYLLGDLICVKLNQPQKNIDLQFDQLAQNYNLESLFSLYEELQQSKRLVEQNVQTQLIIDQLFIKLMNV
>Mature_311_residues
MWETLTTRFPNIGHGLLFYGKQGCGKHAFAKHFLAWVLCLNKQPQGACGECSSCQWLKSDTHPNYVHITTDEENKKQNAK
IKIEKIRDLLPFVQQTGEGWRVVVIEPAEALNLASSNALLKTLEEPGERVVLILLADHYLKLPATIRSRLQHFALDRISY
TEATSYLNEHLAETASVPPELLLGLSNDMPLQAVEIATSDWFAKRQSFLEDWTKVVNQKNMPLFYSAKWQKELNFSDFMM
LLEYLLGDLICVKLNQPQKNIDLQFDQLAQNYNLESLFSLYEELQQSKRLVEQNVQTQLIIDQLFIKLMNV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=147, Percent_Identity=34.0136054421769, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 35789; Mature: 35789

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWETLTTRFPNIGHGLLFYGKQGCGKHAFAKHFLAWVLCLNKQPQGACGECSSCQWLKSD
CCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCC
THPNYVHITTDEENKKQNAKIKIEKIRDLLPFVQQTGEGWRVVVIEPAEALNLASSNALL
CCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHH
KTLEEPGERVVLILLADHYLKLPATIRSRLQHFALDRISYTEATSYLNEHLAETASVPPE
HHHHCCCCEEEEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
LLLGLSNDMPLQAVEIATSDWFAKRQSFLEDWTKVVNQKNMPLFYSAKWQKELNFSDFMM
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHH
LLEYLLGDLICVKLNQPQKNIDLQFDQLAQNYNLESLFSLYEELQQSKRLVEQNVQTQLI
HHHHHHCCEEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHH
IDQLFIKLMNV
HHHHHHHHHCC
>Mature Secondary Structure
MWETLTTRFPNIGHGLLFYGKQGCGKHAFAKHFLAWVLCLNKQPQGACGECSSCQWLKSD
CCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCC
THPNYVHITTDEENKKQNAKIKIEKIRDLLPFVQQTGEGWRVVVIEPAEALNLASSNALL
CCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHH
KTLEEPGERVVLILLADHYLKLPATIRSRLQHFALDRISYTEATSYLNEHLAETASVPPE
HHHHCCCCEEEEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
LLLGLSNDMPLQAVEIATSDWFAKRQSFLEDWTKVVNQKNMPLFYSAKWQKELNFSDFMM
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHH
LLEYLLGDLICVKLNQPQKNIDLQFDQLAQNYNLESLFSLYEELQQSKRLVEQNVQTQLI
HHHHHHCCEEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHH
IDQLFIKLMNV
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]