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The map label for this gene is rlmI [H]

Identifier: 21245076

GI number: 21245076

Start: 5160167

End: 5161333

Strand: Reverse

Name: rlmI [H]

Synonym: XAC4364

Alternate gene names: 21245076

Gene position: 5161333-5160167 (Counterclockwise)

Preceding gene: 21245079

Following gene: 21245075

Centisome position: 99.73

GC content: 64.52

Gene sequence:

>1167_bases
ATGAATATTCCAGTCCCCGTCGTCCGTCTCAAGAATGCCTGGCGCTCCAGCCACCCGTGGATCTTCCAGAAGCTGGTGGA
AAAGCCCGCCGCCAAGCCCAAGCCCGGCAGCATCGTCGACGTGGTCGGCATGGACGGGGAATGGATTGGCCGCGGCTTCT
ACAACGGCCATTCGCGCATTGCCGTGCGCATCCTGGAAACCGACCGGTCGGTGCCGGTGGATGCGGGCTGGTTCTCGCGC
AAGATCGCCGCAGCGGTGAGCCTGCGCCGCGACTGGCTCAAGCTCGACGGCGTGTCCGACGCCTGGCGCGTGGTGCATAG
CGAAGGCGACGGCCTGTCCGGCCTGGTGGTGGACCGCTACGGCGACCTGGTGGTGGTGGAGTTCTTCGCCGCCGGCATGT
TCCGTCACCGCGAATGGATCTACGAGGCGCTGCGCGAACAGTTCCCCGGCTGCCGCTTCCACAGCTTTGCCGACGAACAT
GTGCAGAAGCAGGAAAGCTTCGATTTCCACAGCAACACCACCACCGAAGCGGCGGTGATCACCGAGCACGGCATCAAGTT
CCGCGCCGACCCGGCCGGTGCGCACAAGACCGGTTTCTTCGCCGACCAGCGCGACAACCGTCAGTGGCTGAGCGAGCAGG
TCGAAGGCAAGAGCGTGCTGGACCTGTGCTGCAACACCGGCGGCTTTGCGGTGTATGCCGCCGCGCGCGGTGCGGCCGAA
GTGCTGGGCGTGGACATCGACGAAGACGTGATCGCCATTGCCAAGGGCAACGCCAAGCTCAACAACGTGCGGCCCAAGTT
CGTGCAGGCCGACATCTTTCCGTGGCTGCGCGATGCGGCCAACCGCGGCGAGCAGTTCGATGTGGTGATCCTGGACCCGG
CCAAGATGACCCGCGACCGCGAGCAGGTGATTCCGGCGCTGAAGAAGTATCTGGACATGAACAAGCTGGCCTTGGGCGTG
GTCAAGCCCGGCGGCCTGTTCGCCACGTTCTCGTGTACCGGCCTGGTGGCCGAGCAGGAGTTCCTGGACATGCTGCGGCG
CGCCTCGTATTTCTCCGGGCGCACCATCCAGATTTTGAAAGTGGCCGGTGCCGGCCCGGACCACCCGTTCATGGCGCACG
TGCAGGAATCGCGTTATTTGAAAGCCGTTTTCTGCCGCGTGGTGTAA

Upstream 100 bases:

>100_bases
GCTCGTCATGACGGCGACAACGCTGGGAGCTACTCCCCTTCTGCGCCCGATTCTGCGTGCCCGGCGCTGGCACGTCAATC
CAGCCGGATACAATGGGCCG

Downstream 100 bases:

>100_bases
GCGCGCTCGGTGGCGGAGGACGCGGTCTGCGGCATTGCAGGCAGCGTCTTTCGCGGCGCGATGCCATCGCCGGCAAGCAG
TAGGTCAACGCAGACATGCA

Product: oxidoreductase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MNIPVPVVRLKNAWRSSHPWIFQKLVEKPAAKPKPGSIVDVVGMDGEWIGRGFYNGHSRIAVRILETDRSVPVDAGWFSR
KIAAAVSLRRDWLKLDGVSDAWRVVHSEGDGLSGLVVDRYGDLVVVEFFAAGMFRHREWIYEALREQFPGCRFHSFADEH
VQKQESFDFHSNTTTEAAVITEHGIKFRADPAGAHKTGFFADQRDNRQWLSEQVEGKSVLDLCCNTGGFAVYAAARGAAE
VLGVDIDEDVIAIAKGNAKLNNVRPKFVQADIFPWLRDAANRGEQFDVVILDPAKMTRDREQVIPALKKYLDMNKLALGV
VKPGGLFATFSCTGLVAEQEFLDMLRRASYFSGRTIQILKVAGAGPDHPFMAHVQESRYLKAVFCRVV

Sequences:

>Translated_388_residues
MNIPVPVVRLKNAWRSSHPWIFQKLVEKPAAKPKPGSIVDVVGMDGEWIGRGFYNGHSRIAVRILETDRSVPVDAGWFSR
KIAAAVSLRRDWLKLDGVSDAWRVVHSEGDGLSGLVVDRYGDLVVVEFFAAGMFRHREWIYEALREQFPGCRFHSFADEH
VQKQESFDFHSNTTTEAAVITEHGIKFRADPAGAHKTGFFADQRDNRQWLSEQVEGKSVLDLCCNTGGFAVYAAARGAAE
VLGVDIDEDVIAIAKGNAKLNNVRPKFVQADIFPWLRDAANRGEQFDVVILDPAKMTRDREQVIPALKKYLDMNKLALGV
VKPGGLFATFSCTGLVAEQEFLDMLRRASYFSGRTIQILKVAGAGPDHPFMAHVQESRYLKAVFCRVV
>Mature_388_residues
MNIPVPVVRLKNAWRSSHPWIFQKLVEKPAAKPKPGSIVDVVGMDGEWIGRGFYNGHSRIAVRILETDRSVPVDAGWFSR
KIAAAVSLRRDWLKLDGVSDAWRVVHSEGDGLSGLVVDRYGDLVVVEFFAAGMFRHREWIYEALREQFPGCRFHSFADEH
VQKQESFDFHSNTTTEAAVITEHGIKFRADPAGAHKTGFFADQRDNRQWLSEQVEGKSVLDLCCNTGGFAVYAAARGAAE
VLGVDIDEDVIAIAKGNAKLNNVRPKFVQADIFPWLRDAANRGEQFDVVILDPAKMTRDREQVIPALKKYLDMNKLALGV
VKPGGLFATFSCTGLVAEQEFLDMLRRASYFSGRTIQILKVAGAGPDHPFMAHVQESRYLKAVFCRVV

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=379, Percent_Identity=35.0923482849604, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1787181, Length=224, Percent_Identity=26.3392857142857, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43333; Mature: 43333

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIPVPVVRLKNAWRSSHPWIFQKLVEKPAAKPKPGSIVDVVGMDGEWIGRGFYNGHSRI
CCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCEECCCEECCCCEE
AVRILETDRSVPVDAGWFSRKIAAAVSLRRDWLKLDGVSDAWRVVHSEGDGLSGLVVDRY
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCHHHHHEECCCCCCCEEEEECC
GDLVVVEFFAAGMFRHREWIYEALREQFPGCRFHSFADEHVQKQESFDFHSNTTTEAAVI
CCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEE
TEHGIKFRADPAGAHKTGFFADQRDNRQWLSEQVEGKSVLDLCCNTGGFAVYAAARGAAE
EECCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCHH
VLGVDIDEDVIAIAKGNAKLNNVRPKFVQADIFPWLRDAANRGEQFDVVILDPAKMTRDR
HEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHCCCH
EQVIPALKKYLDMNKLALGVVKPGGLFATFSCTGLVAEQEFLDMLRRASYFSGRTIQILK
HHHHHHHHHHHCCCHHEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEE
VAGAGPDHPFMAHVQESRYLKAVFCRVV
EECCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIPVPVVRLKNAWRSSHPWIFQKLVEKPAAKPKPGSIVDVVGMDGEWIGRGFYNGHSRI
CCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCEECCCEECCCCEE
AVRILETDRSVPVDAGWFSRKIAAAVSLRRDWLKLDGVSDAWRVVHSEGDGLSGLVVDRY
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCHHHHHEECCCCCCCEEEEECC
GDLVVVEFFAAGMFRHREWIYEALREQFPGCRFHSFADEHVQKQESFDFHSNTTTEAAVI
CCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEE
TEHGIKFRADPAGAHKTGFFADQRDNRQWLSEQVEGKSVLDLCCNTGGFAVYAAARGAAE
EECCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCHH
VLGVDIDEDVIAIAKGNAKLNNVRPKFVQADIFPWLRDAANRGEQFDVVILDPAKMTRDR
HEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHCCCH
EQVIPALKKYLDMNKLALGVVKPGGLFATFSCTGLVAEQEFLDMLRRASYFSGRTIQILK
HHHHHHHHHHHCCCHHEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEE
VAGAGPDHPFMAHVQESRYLKAVFCRVV
EECCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA