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The map label for this gene is btuE [H]

Identifier: 21245058

GI number: 21245058

Start: 5139249

End: 5139800

Strand: Reverse

Name: btuE [H]

Synonym: XAC4346

Alternate gene names: 21245058

Gene position: 5139800-5139249 (Counterclockwise)

Preceding gene: 77748776

Following gene: 21245057

Centisome position: 99.31

GC content: 65.04

Gene sequence:

>552_bases
ATGAGCCAGACGCTATACGACATTCCCGTGACCCGCATCGATGGCCAGCCCACCACCTTGGCGGACTATCGCGGCAAGGT
GCTGCTGGTGGTCAACGTCGCCTCCAAGTGCGGCCTGACCCCGCAGTACGAAGGGCTGGAAGCGCTGTATCGCGACAAGC
GCGCACAGGGCCTGGAAGTGCTGGCGTTTCCGGCCAACGACTTCAACGGCCAGGAGCCGGGCAGCGAAGCGGAGATCGCG
CAGTTCTGCCAGCTCACCTACGACGTCACCTTCCCGATGTTTGCCAAGATCGCCGTCGCTGGCGACCAGGCGCACCCGCT
GTATGGGGCACTGACCAGCACCCATCCGCACACCACCGGCGAGGGCCCGATGCGCGAGAAACTTGCCGGTTACGGCATCG
CCCCCAACCCCGCGCCGGGCGTGGTGTGGAACTTCGAAAAATTCCTGATCGGTCGCGACGGCAAGATCATCGACCGCTTC
GCCCCGGACATTCCTGCCGACGATGCGCGCTTGCGTGCCGCGATTGATGCGGCGCTGGCCACACGCTCATAG

Upstream 100 bases:

>100_bases
GAGCGTCGCTGCAGACAACTGCCAGCCGCGTGAGCCAGCGGCATCGCTCGCCTCACCGTGGCCGTTTATGCTCTGGGCTC
TCCCCGCCCAGGACGCACCG

Downstream 100 bases:

>100_bases
AGCGCTGTCGGCATTGCGATGACGCGTCATGTCCTTATCGGGAGCCGGCGAATAGGCAATGACCTTCAAGTACTTCTTCA
GCTCTTCAATGCTCGCGTTG

Product: glutathione peroxidase

Products: ADP; phosphate; cob(I)alamin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MSQTLYDIPVTRIDGQPTTLADYRGKVLLVVNVASKCGLTPQYEGLEALYRDKRAQGLEVLAFPANDFNGQEPGSEAEIA
QFCQLTYDVTFPMFAKIAVAGDQAHPLYGALTSTHPHTTGEGPMREKLAGYGIAPNPAPGVVWNFEKFLIGRDGKIIDRF
APDIPADDARLRAAIDAALATRS

Sequences:

>Translated_183_residues
MSQTLYDIPVTRIDGQPTTLADYRGKVLLVVNVASKCGLTPQYEGLEALYRDKRAQGLEVLAFPANDFNGQEPGSEAEIA
QFCQLTYDVTFPMFAKIAVAGDQAHPLYGALTSTHPHTTGEGPMREKLAGYGIAPNPAPGVVWNFEKFLIGRDGKIIDRF
APDIPADDARLRAAIDAALATRS
>Mature_182_residues
SQTLYDIPVTRIDGQPTTLADYRGKVLLVVNVASKCGLTPQYEGLEALYRDKRAQGLEVLAFPANDFNGQEPGSEAEIAQ
FCQLTYDVTFPMFAKIAVAGDQAHPLYGALTSTHPHTTGEGPMREKLAGYGIAPNPAPGVVWNFEKFLIGRDGKIIDRFA
PDIPADDARLRAAIDAALATRS

Specific function: Not essential for B12 transport; however, it is an auxiliary component of the transport system [H]

COG id: COG0386

COG function: function code O; Glutathione peroxidase

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutathione peroxidase family [H]

Homologues:

Organism=Homo sapiens, GI15618997, Length=169, Percent_Identity=40.8284023668639, Blast_Score=125, Evalue=2e-29,
Organism=Homo sapiens, GI90903240, Length=162, Percent_Identity=38.8888888888889, Blast_Score=107, Evalue=7e-24,
Organism=Homo sapiens, GI75709200, Length=162, Percent_Identity=38.8888888888889, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI192455698, Length=169, Percent_Identity=31.9526627218935, Blast_Score=94, Evalue=8e-20,
Organism=Homo sapiens, GI90903238, Length=148, Percent_Identity=39.1891891891892, Blast_Score=93, Evalue=1e-19,
Organism=Homo sapiens, GI4557629, Length=164, Percent_Identity=32.9268292682927, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI6006001, Length=177, Percent_Identity=33.3333333333333, Blast_Score=85, Evalue=3e-17,
Organism=Homo sapiens, GI41406084, Length=156, Percent_Identity=35.8974358974359, Blast_Score=81, Evalue=4e-16,
Organism=Homo sapiens, GI33186887, Length=175, Percent_Identity=30.8571428571429, Blast_Score=76, Evalue=1e-14,
Organism=Homo sapiens, GI32967607, Length=175, Percent_Identity=30.2857142857143, Blast_Score=74, Evalue=9e-14,
Organism=Escherichia coli, GI1788003, Length=182, Percent_Identity=47.2527472527472, Blast_Score=169, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17506887, Length=180, Percent_Identity=38.8888888888889, Blast_Score=120, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17535473, Length=180, Percent_Identity=38.3333333333333, Blast_Score=120, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17550320, Length=170, Percent_Identity=35.8823529411765, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI193209493, Length=186, Percent_Identity=32.258064516129, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71993584, Length=165, Percent_Identity=32.7272727272727, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI193210707, Length=164, Percent_Identity=33.5365853658537, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71993573, Length=98, Percent_Identity=37.7551020408163, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71999642, Length=78, Percent_Identity=39.7435897435897, Blast_Score=66, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6322228, Length=161, Percent_Identity=44.7204968944099, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319721, Length=161, Percent_Identity=40.9937888198758, Blast_Score=139, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6322826, Length=159, Percent_Identity=36.4779874213836, Blast_Score=116, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24656772, Length=177, Percent_Identity=37.2881355932203, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24656767, Length=177, Percent_Identity=37.2881355932203, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24656777, Length=177, Percent_Identity=37.2881355932203, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI85726443, Length=163, Percent_Identity=37.4233128834356, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000889
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00255 GSHPx [H]

EC number: NA

Molecular weight: Translated: 19809; Mature: 19677

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00460 GLUTATHIONE_PEROXID_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTLYDIPVTRIDGQPTTLADYRGKVLLVVNVASKCGLTPQYEGLEALYRDKRAQGLEV
CCCEEECCCEEEECCCCCCHHCCCCCEEEEEEEHHHCCCCCCCHHHHHHHHHHHCCCCEE
LAFPANDFNGQEPGSEAEIAQFCQLTYDVTFPMFAKIAVAGDQAHPLYGALTSTHPHTTG
EEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCEEEECCCCCCCCC
EGPMREKLAGYGIAPNPAPGVVWNFEKFLIGRDGKIIDRFAPDIPADDARLRAAIDAALA
CCHHHHHHHCCCCCCCCCCCEEECHHHEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHH
TRS
CCC
>Mature Secondary Structure 
SQTLYDIPVTRIDGQPTTLADYRGKVLLVVNVASKCGLTPQYEGLEALYRDKRAQGLEV
CCEEECCCEEEECCCCCCHHCCCCCEEEEEEEHHHCCCCCCCHHHHHHHHHHHCCCCEE
LAFPANDFNGQEPGSEAEIAQFCQLTYDVTFPMFAKIAVAGDQAHPLYGALTSTHPHTTG
EEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCEEEECCCCCCCCC
EGPMREKLAGYGIAPNPAPGVVWNFEKFLIGRDGKIIDRFAPDIPADDARLRAAIDAALA
CCHHHHHHHCCCCCCCCCCCEEECHHHEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHH
TRS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; cob(I)alamin [Periplasm]; H2O [C]

Specific reaction: ATP + cob(I)alamin [Periplasm] + H2O = ADP + phosphate + cob(I)alamin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3528129; 9097039; 9278503 [H]