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The map label for this gene is wbnF [C]

Identifier: 21244627

GI number: 21244627

Start: 4593241

End: 4594206

Strand: Direct

Name: wbnF [C]

Synonym: XAC3908

Alternate gene names: 21244627

Gene position: 4593241-4594206 (Clockwise)

Preceding gene: 21244626

Following gene: 21244628

Centisome position: 88.75

GC content: 66.77

Gene sequence:

>966_bases
ATGACCATTCTCGTCACCGGCGCTGCCGGTTTCATCGGTGCCTACACCTGCCGCGCGCTGGCGGCACGCGGCGAGACGGT
GGTGGGGCTGGACAACTACAACAACTACTACGACCCGCAGCTCAAGCACGACCGGGTGGCGACGCTGTGCCCAGGCCTGG
ATATCCGCACGCTGGACCTGACCGATCGCGAGGGCCTGGCTGCATTGTTCGATGAGATCCAGCCGACCCGCGTCGTGCAT
CTGGCTGCGCAGGCGGGCGTGCGGTATTCGCTGGAAAACCCCCACGCCTACGTCGACAGCAACCTGGTCGGCTTCGTCAA
CATGCTCGAGCTGTGCCGGCACCGCGGCGTGCAGCACCTGGTGTATGCCTCGAGCAGTTCGGTCTACGGCGATTCGGCCA
CCCCGCCGTTTTCCGAAGACCAGCGCGTGGACCAACCGCGTTCGTTGTATGCGGCAACCAAGGCCGCCAACGAATTGATG
GGCCACACCTATGCGCAGCTGTATGGCCTGCGCGCGACCGGTCTGCGCTTTTTCACGGTATACGGGCCGTGGGGCCGGCC
GGACATGGCACCGCTGATCTTCAGCCGTGCGGTGCTGGCGGGGCGGCCGATCGAGGTGTTCAACCACGGCAAGATGCAGC
GCGATTTCACTTTCGTCGACGATATCGTGGCCGGTGTGCTGGGCGCGCTGGACACGCCCAGCGACGCGCCGGTGCCGCAC
CGGGTGTTCAACCTGGGCAACCACACGCCGGTGGAGCTGGAGTACTTCATCGACGTGATCGCGCAGGCCGCCGGCCGCCC
GGCCGAGAAGGTCTACCGGCCGATGCAGCCGGGCGACATGATCCGCACCATGGCCGATACGCAGCGTGCGCAGGCCGCCT
TCGGGTTCGATCCGGCGACCCCGGTCGAGCGTGGCTTGCCGCAGGTCGTGGAGTGGTGCCGGCAGTATTTCGGCGAGCGC
GCCTAG

Upstream 100 bases:

>100_bases
ATCGGGATGCCGGACCCGCTCGCGGCGGTGTCCGCAATGCGCCGCGCGTGCGCATCCGGAGCGCCAGCCTTTTGTTCCCG
CATTTCCCAGGATCACAGCC

Downstream 100 bases:

>100_bases
CGCTTGGCGGGGGTTGCTGCGGAGTTGCACGTGGGCGTCGATGGGCGCGCGTAGCGCCGGGACTGCCATGCGGGCCAGAT
GCAGGGCGTACTACTGTCCA

Product: nucleotide sugar epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTILVTGAAGFIGAYTCRALAARGETVVGLDNYNNYYDPQLKHDRVATLCPGLDIRTLDLTDREGLAALFDEIQPTRVVH
LAAQAGVRYSLENPHAYVDSNLVGFVNMLELCRHRGVQHLVYASSSSVYGDSATPPFSEDQRVDQPRSLYAATKAANELM
GHTYAQLYGLRATGLRFFTVYGPWGRPDMAPLIFSRAVLAGRPIEVFNHGKMQRDFTFVDDIVAGVLGALDTPSDAPVPH
RVFNLGNHTPVELEYFIDVIAQAAGRPAEKVYRPMQPGDMIRTMADTQRAQAAFGFDPATPVERGLPQVVEWCRQYFGER
A

Sequences:

>Translated_321_residues
MTILVTGAAGFIGAYTCRALAARGETVVGLDNYNNYYDPQLKHDRVATLCPGLDIRTLDLTDREGLAALFDEIQPTRVVH
LAAQAGVRYSLENPHAYVDSNLVGFVNMLELCRHRGVQHLVYASSSSVYGDSATPPFSEDQRVDQPRSLYAATKAANELM
GHTYAQLYGLRATGLRFFTVYGPWGRPDMAPLIFSRAVLAGRPIEVFNHGKMQRDFTFVDDIVAGVLGALDTPSDAPVPH
RVFNLGNHTPVELEYFIDVIAQAAGRPAEKVYRPMQPGDMIRTMADTQRAQAAFGFDPATPVERGLPQVVEWCRQYFGER
A
>Mature_320_residues
TILVTGAAGFIGAYTCRALAARGETVVGLDNYNNYYDPQLKHDRVATLCPGLDIRTLDLTDREGLAALFDEIQPTRVVHL
AAQAGVRYSLENPHAYVDSNLVGFVNMLELCRHRGVQHLVYASSSSVYGDSATPPFSEDQRVDQPRSLYAATKAANELMG
HTYAQLYGLRATGLRFFTVYGPWGRPDMAPLIFSRAVLAGRPIEVFNHGKMQRDFTFVDDIVAGVLGALDTPSDAPVPHR
VFNLGNHTPVELEYFIDVIAQAAGRPAEKVYRPMQPGDMIRTMADTQRAQAAFGFDPATPVERGLPQVVEWCRQYFGERA

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=322, Percent_Identity=27.9503105590062, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI56237023, Length=337, Percent_Identity=26.4094955489614, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI56118217, Length=337, Percent_Identity=26.4094955489614, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI189083684, Length=337, Percent_Identity=26.4094955489614, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI7657641, Length=337, Percent_Identity=23.4421364985163, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI48994969, Length=334, Percent_Identity=26.9461077844311, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788353, Length=343, Percent_Identity=27.4052478134111, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786974, Length=318, Percent_Identity=25.4716981132075, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788366, Length=199, Percent_Identity=28.1407035175879, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71982038, Length=343, Percent_Identity=27.4052478134111, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71982035, Length=338, Percent_Identity=27.2189349112426, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17539532, Length=319, Percent_Identity=25.3918495297806, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17568069, Length=334, Percent_Identity=25.748502994012, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI115532424, Length=325, Percent_Identity=24.6153846153846, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17554428, Length=325, Percent_Identity=21.5384615384615, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=343, Percent_Identity=26.2390670553936, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356223, Length=318, Percent_Identity=27.3584905660377, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19923002, Length=334, Percent_Identity=26.6467065868263, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 35427; Mature: 35295

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILVTGAAGFIGAYTCRALAARGETVVGLDNYNNYYDPQLKHDRVATLCPGLDIRTLDL
CEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCCEEEECC
TDREGLAALFDEIQPTRVVHLAAQAGVRYSLENPHAYVDSNLVGFVNMLELCRHRGVQHL
CCCCHHHHHHHCCCCHHEEEEHHHCCCEEECCCCCEECCCHHHHHHHHHHHHHHCCCEEE
VYASSSSVYGDSATPPFSEDQRVDQPRSLYAATKAANELMGHTYAQLYGLRATGLRFFTV
EEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
YGPWGRPDMAPLIFSRAVLAGRPIEVFNHGKMQRDFTFVDDIVAGVLGALDTPSDAPVPH
ECCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCH
RVFNLGNHTPVELEYFIDVIAQAAGRPAEKVYRPMQPGDMIRTMADTQRAQAAFGFDPAT
HHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCC
PVERGLPQVVEWCRQYFGERA
HHHHCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TILVTGAAGFIGAYTCRALAARGETVVGLDNYNNYYDPQLKHDRVATLCPGLDIRTLDL
EEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCCEEEECC
TDREGLAALFDEIQPTRVVHLAAQAGVRYSLENPHAYVDSNLVGFVNMLELCRHRGVQHL
CCCCHHHHHHHCCCCHHEEEEHHHCCCEEECCCCCEECCCHHHHHHHHHHHHHHCCCEEE
VYASSSSVYGDSATPPFSEDQRVDQPRSLYAATKAANELMGHTYAQLYGLRATGLRFFTV
EEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
YGPWGRPDMAPLIFSRAVLAGRPIEVFNHGKMQRDFTFVDDIVAGVLGALDTPSDAPVPH
ECCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCH
RVFNLGNHTPVELEYFIDVIAQAAGRPAEKVYRPMQPGDMIRTMADTQRAQAAFGFDPAT
HHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCC
PVERGLPQVVEWCRQYFGERA
HHHHCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]