Click here to switch to the map view.

The map label for this gene is tcbD [C]

Identifier: 21244586

GI number: 21244586

Start: 4545367

End: 4546464

Strand: Direct

Name: tcbD [C]

Synonym: XAC3862

Alternate gene names: 21244586

Gene position: 4545367-4546464 (Clockwise)

Preceding gene: 21244585

Following gene: 21244587

Centisome position: 87.82

GC content: 65.39

Gene sequence:

>1098_bases
ATGAAGATCACCGGCTTCCGGCTTGGCATGGTGCGCGTGCCCCTGAAAACACCGTTCAAGACGGCCGTGCGCACGGTGCA
GGCAATCGAGGACGTGGTGGTGCTGTTGCAGACCGACAGCGGCCACATCGGGTACGGCGCCGCGCCCGCCACCGCACCCA
TCACCGGCGATACCCATGGCAGCATCGTTGCCGCGATCGAACACTGCATCGGCCCCAAGCTGATCGGACAGGACGTAGCC
GATCTCAACCGGCTATGCGGGCTCGTTCAACACGCGCTCGAACGCAACACCAGCGCCAAGGCCGCGGTGGAAATCGCACT
GTACGATCTGTGGGCGCAGGGTTTTGGTGCGCCGCTGTACCAGTTGCTCGGTGGCGGCACGCCGCGCGTCACGACCGACA
TCACCATCAGCGCCGATACCATCGACACCATGGTGGCGCAGGCGCAAGCGGCGCTCGCGCACGGCTATCGCGCACTGAAG
ATCAAGGTCGGCAAGGACATCGAGTCGGATGTCGAACGCGTCACGGCAATTCATGCCGCAGTCGCTGGGCGCGCATCGCT
GCGGCTGGATGCCAATCAAGGCTGGACGCCCAAACAAGCGGTGCGCACCATGCGTACGCTGGAAGATGGCGGCATCGCGC
TGGGGCTGCTGGAGCAGCCGGTCAAGGCCGCCGACCTCGACGGGCTCGCCTTCGTCACCGCACGCATCGACACGCCGGTG
ATGGCCGACGAGAGCGTCTTCTCGCCCATGCAGGTGATCGCGCTGATTCAGCGTCGCGCCGCCGACATCATCAACATCAA
ACTGATGAAGACCGGCGGGCTGTCCAATGCCATCCGCATCGCCGACATCGCCGGGTTGTACGGGTTGCCATGCATGATCG
GCTGCATGATCGAATCCAGCATCAGCGTGGCCGCCGCGGTGCATCTGGCGGTGGCCAAGGCCGACAGCATCACCCTGGCC
GATCTGGATGCGCCCGCGCTTGGGCAGTTCGACCCCACCGAGGGCGGCGTGCGGTTCGACGAGGCACAGATCCACATCGA
CGATTCGCCGGGACTGGGCATCCGCCGCATTCGTGGGCTGGAGCTGTTGCCGGCGTGA

Upstream 100 bases:

>100_bases
GTGTGTCGGTCACCCCATTGCTGCCCTTGCTCGCCAGCGATGGCACTGCATTCATCGACCACATCACCCGCATTCAAAGG
GTTTTTCCAACGGGCACATT

Downstream 100 bases:

>100_bases
GAGCGGCCGACGCGGGCGGCGAACAGCCCCCCAAGTGGAGGCAGACCGCGCTGTAACGTTGGTTTGACCAGCGTTACTCG
CCGATTCCGAGCAAGGGACG

Product: chloromuconate cycloisomerase

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKITGFRLGMVRVPLKTPFKTAVRTVQAIEDVVVLLQTDSGHIGYGAAPATAPITGDTHGSIVAAIEHCIGPKLIGQDVA
DLNRLCGLVQHALERNTSAKAAVEIALYDLWAQGFGAPLYQLLGGGTPRVTTDITISADTIDTMVAQAQAALAHGYRALK
IKVGKDIESDVERVTAIHAAVAGRASLRLDANQGWTPKQAVRTMRTLEDGGIALGLLEQPVKAADLDGLAFVTARIDTPV
MADESVFSPMQVIALIQRRAADIINIKLMKTGGLSNAIRIADIAGLYGLPCMIGCMIESSISVAAAVHLAVAKADSITLA
DLDAPALGQFDPTEGGVRFDEAQIHIDDSPGLGIRRIRGLELLPA

Sequences:

>Translated_365_residues
MKITGFRLGMVRVPLKTPFKTAVRTVQAIEDVVVLLQTDSGHIGYGAAPATAPITGDTHGSIVAAIEHCIGPKLIGQDVA
DLNRLCGLVQHALERNTSAKAAVEIALYDLWAQGFGAPLYQLLGGGTPRVTTDITISADTIDTMVAQAQAALAHGYRALK
IKVGKDIESDVERVTAIHAAVAGRASLRLDANQGWTPKQAVRTMRTLEDGGIALGLLEQPVKAADLDGLAFVTARIDTPV
MADESVFSPMQVIALIQRRAADIINIKLMKTGGLSNAIRIADIAGLYGLPCMIGCMIESSISVAAAVHLAVAKADSITLA
DLDAPALGQFDPTEGGVRFDEAQIHIDDSPGLGIRRIRGLELLPA
>Mature_365_residues
MKITGFRLGMVRVPLKTPFKTAVRTVQAIEDVVVLLQTDSGHIGYGAAPATAPITGDTHGSIVAAIEHCIGPKLIGQDVA
DLNRLCGLVQHALERNTSAKAAVEIALYDLWAQGFGAPLYQLLGGGTPRVTTDITISADTIDTMVAQAQAALAHGYRALK
IKVGKDIESDVERVTAIHAAVAGRASLRLDANQGWTPKQAVRTMRTLEDGGIALGLLEQPVKAADLDGLAFVTARIDTPV
MADESVFSPMQVIALIQRRAADIINIKLMKTGGLSNAIRIADIAGLYGLPCMIGCMIESSISVAAAVHLAVAKADSITLA
DLDAPALGQFDPTEGGVRFDEAQIHIDDSPGLGIRRIRGLELLPA

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the epimerization of L-Ala-D-Asp [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=337, Percent_Identity=31.7507418397626, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 38385; Mature: 38385

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITGFRLGMVRVPLKTPFKTAVRTVQAIEDVVVLLQTDSGHIGYGAAPATAPITGDTHG
CEECEEEEEEEEECCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCC
SIVAAIEHCIGPKLIGQDVADLNRLCGLVQHALERNTSAKAAVEIALYDLWAQGFGAPLY
HHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHEEHHHHHHHHHCCCCHHH
QLLGGGTPRVTTDITISADTIDTMVAQAQAALAHGYRALKIKVGKDIESDVERVTAIHAA
HHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHH
VAGRASLRLDANQGWTPKQAVRTMRTLEDGGIALGLLEQPVKAADLDGLAFVTARIDTPV
HCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEHHHCCCCCCCCCCEEEEEEECCCCC
MADESVFSPMQVIALIQRRAADIINIKLMKTGGLSNAIRIADIAGLYGLPCMIGCMIESS
CCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCC
ISVAAAVHLAVAKADSITLADLDAPALGQFDPTEGGVRFDEAQIHIDDSPGLGIRRIRGL
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEECEEEEECCCCCCCHHHCCCE
ELLPA
ECCCC
>Mature Secondary Structure
MKITGFRLGMVRVPLKTPFKTAVRTVQAIEDVVVLLQTDSGHIGYGAAPATAPITGDTHG
CEECEEEEEEEEECCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCC
SIVAAIEHCIGPKLIGQDVADLNRLCGLVQHALERNTSAKAAVEIALYDLWAQGFGAPLY
HHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHEEHHHHHHHHHCCCCHHH
QLLGGGTPRVTTDITISADTIDTMVAQAQAALAHGYRALKIKVGKDIESDVERVTAIHAA
HHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHH
VAGRASLRLDANQGWTPKQAVRTMRTLEDGGIALGLLEQPVKAADLDGLAFVTARIDTPV
HCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEHHHCCCCCCCCCCEEEEEEECCCCC
MADESVFSPMQVIALIQRRAADIINIKLMKTGGLSNAIRIADIAGLYGLPCMIGCMIESS
CCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCC
ISVAAAVHLAVAKADSITLADLDAPALGQFDPTEGGVRFDEAQIHIDDSPGLGIRRIRGL
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEECEEEEECCCCCCCHHHCCCE
ELLPA
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]