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The map label for this gene is priA [H]

Identifier: 21244542

GI number: 21244542

Start: 4491394

End: 4493616

Strand: Direct

Name: priA [H]

Synonym: XAC3818

Alternate gene names: 21244542

Gene position: 4491394-4493616 (Clockwise)

Preceding gene: 21244539

Following gene: 21244546

Centisome position: 86.78

GC content: 71.97

Gene sequence:

>2223_bases
ATGTCTTCGACCGTCGCCACCCTGCGCGTCGCCTTGCCGGTGCCGCTGCCCCAGTTGTTCGACTATCTGCCTCCGGCCGA
TGCGGCGCCGACCGATCCGGCGCGGGTCGGCTGCCGGGTGCGGGTGCCGTTCGGGCCGCGCGAGCTGGTCGGGGTTGTGG
TGGAGATCGGGCAACTGCCCGCGGCCGACGGCCTGCGCCCGGCACTGGCGTGGTGCGACCAGGCCCCGCTGCTGGTCGAT
GAACTGGCCCGCTCGCTGCACTGGCTGGCCCGCTACACCCACGCCCCGCTCGGCGAGGCCCAGGCCAGCGCCCTGCCCGG
CCCGCTTCGGCGCGGCGAGCCGCTGGCCGATACCCACGCCTGGGCCTGGCAGCTGACCGAAGCCGGCCGCACCGGCTCCA
CCAGCCTGCGCGCAGGCAGCCGCCCCGCCCTGCTGGCAGCCCTGCTGCGCACCGGTGCGGTCGGCGAGGAGCAACTGGAC
CCGCTGCTGCCGCAGTGGCGCGAAGCCGCGCGCAGCCTGGCCAAGCGCGAGTACGCCGAGCGCGTGGCGGTGCCGGCCGA
CACGATTCCGCCACGCCCCGGCAACGGCCCGCCGCTCAACGACGAACAACAGGCCGCCACCGCCGCGATCCGCGCGCATA
GCGGCTTTGCCACCTATCTGCTCGACGGCGTCACCGGCAGCGGCAAGACCGAGGTCTACCTGCAGGCGATCGCCGATTGC
CTGGCCGCCGGCAAGCAGGCGCTGGTGCTGGTGCCCGAGATCGGGCTGACGCCGCAGACCCTGGGGCGTTTCCGTGCGCG
CCTGGGCGTGCCGGTGCATGCGCTGCACTCGGGTCTGTCCGATGGCGAACGCGCGCGGGTTTGGGCGGCGGCCTGGCGCG
GCCAGGCACAGCTGATCGTCGGCACCCGCTCGGCGGTGTTCACCCCGCTGCCCAACGCCGGCCTGATCGTGATCGACGAA
GAACACGACGGCAGCTACAAGCAGCAGGACGGCATCCGCTATCACGCGCGCGATTTCGCGCTGGTGCGCGGCAAGGCGCT
GGATGTGCCGGTGATCCTGGGCAGCGCCACGCCATCGCTGGAAAGCCTGCACAACGCCTACGCCGGTCGCTACCAGCATC
TGCGCCTGTCGCAGCGCGCCGGCGAAGCGCGTCCGCCGCGCGTGCGCGTGCTCGACGTGCGCAAGCGCCCGCTCAAGGAC
GGCCTGTCGCCCGAGGTGCTGGCCGGTATCGGCACCACCCTGGCGCGTGGCGAACAGGTCCTGGTGTTCAAGAACCGCCG
CGGCTATGCACCGGTGCTGCTGTGCCACGACTGCGGCTGGACCGCTGCCTGCCAGCGCTGCAGCACGCCGCTGCACCAGA
CCCCGATGACCGTGCATGCAGGCGGCCGGCGCCTGCAGTGCCACCATTGCGGCGCGCGCCAACCCGCACCGCTGGCCTGC
CCGGCCTGCGCCAGCCTGGCGCTGCAGCCGCAGGGCATCGGCACCGAGCGGCTGGAAGAACGCCTGACCGAGGCGTTTCC
GGACGTCCCGGTGGTGCGCATCGACCGCAGCACCACCCAGCGCCGCGACGCACTGGAAACCCAGCTCGCCCGGCTCGGCA
CCGAGGCCGGCATCCTGGTCGGCACGCAGATCCTGGCCAAGGGCCACGACCTGCCGCGGCTAACCATGGTCGTGGTGGTC
GGCATCGACGAAGGCCTGTTCTCGGCCGACTTCCGCGCCGCCGAAAAACTGGCCCAGCAGTTGATCCAGGTGGCCGGACG
CGCCGGCCGTGCCGACCGGCCGGGCGAAGTCTGGCTGCAGACCCATCACCCCGAACACCCGTTGCTGCAGACCCTGGTCA
ATGGCGGCTACCACGCCTTCGCCGACGCCGAACTGCAGCAGCGCGAGGCCGCGGGATTTCCGCCGTTCGCGCATCTGGCG
CTGTTCCGCGCCGAGGCCAAGGACGTGGCCGCGGCCAACCAATTCCTGATGGCGGTGCGTGGCCTGACCACGGCAGACAG
CACCGCACAATCGCCTGCCTTTGCCGCAGTGGAGTGCTACGGCCCGATGCCGGCACCGATGCCACGCCGCGCCGGTTTCC
AGCGCAGCCAGTTGCTGTTGTCCGCGCAACAGCGCTCGGCGCTGCATCGTCTGCTGGATGCGCAACTGCCTGCGATCTAT
GCGCTGCCGCAGGCGCGGCGCGTACGCTGGTCGCTGGATGTGGATCCGATCGATCTGTATTAG

Upstream 100 bases:

>100_bases
TATCACTTCCGTCACAGAAACGCGGCCGCTGCCCCGGCGTGGATCTGGGCCGATTCGGGGTCGGCCGCCGGCCTCTGGAC
AGGGTGAGTAGACTGGTCGG

Downstream 100 bases:

>100_bases
TGCGGGCGTATCGCACGCGGAGCAGCAGCTAGATCACCGTGTACCCAGCTTGCGTAGGAGCGCACCTGGGCGCGATGTGG
CGTTCCCGGGAATGCCCTCG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 740; Mature: 739

Protein sequence:

>740_residues
MSSTVATLRVALPVPLPQLFDYLPPADAAPTDPARVGCRVRVPFGPRELVGVVVEIGQLPAADGLRPALAWCDQAPLLVD
ELARSLHWLARYTHAPLGEAQASALPGPLRRGEPLADTHAWAWQLTEAGRTGSTSLRAGSRPALLAALLRTGAVGEEQLD
PLLPQWREAARSLAKREYAERVAVPADTIPPRPGNGPPLNDEQQAATAAIRAHSGFATYLLDGVTGSGKTEVYLQAIADC
LAAGKQALVLVPEIGLTPQTLGRFRARLGVPVHALHSGLSDGERARVWAAAWRGQAQLIVGTRSAVFTPLPNAGLIVIDE
EHDGSYKQQDGIRYHARDFALVRGKALDVPVILGSATPSLESLHNAYAGRYQHLRLSQRAGEARPPRVRVLDVRKRPLKD
GLSPEVLAGIGTTLARGEQVLVFKNRRGYAPVLLCHDCGWTAACQRCSTPLHQTPMTVHAGGRRLQCHHCGARQPAPLAC
PACASLALQPQGIGTERLEERLTEAFPDVPVVRIDRSTTQRRDALETQLARLGTEAGILVGTQILAKGHDLPRLTMVVVV
GIDEGLFSADFRAAEKLAQQLIQVAGRAGRADRPGEVWLQTHHPEHPLLQTLVNGGYHAFADAELQQREAAGFPPFAHLA
LFRAEAKDVAAANQFLMAVRGLTTADSTAQSPAFAAVECYGPMPAPMPRRAGFQRSQLLLSAQQRSALHRLLDAQLPAIY
ALPQARRVRWSLDVDPIDLY

Sequences:

>Translated_740_residues
MSSTVATLRVALPVPLPQLFDYLPPADAAPTDPARVGCRVRVPFGPRELVGVVVEIGQLPAADGLRPALAWCDQAPLLVD
ELARSLHWLARYTHAPLGEAQASALPGPLRRGEPLADTHAWAWQLTEAGRTGSTSLRAGSRPALLAALLRTGAVGEEQLD
PLLPQWREAARSLAKREYAERVAVPADTIPPRPGNGPPLNDEQQAATAAIRAHSGFATYLLDGVTGSGKTEVYLQAIADC
LAAGKQALVLVPEIGLTPQTLGRFRARLGVPVHALHSGLSDGERARVWAAAWRGQAQLIVGTRSAVFTPLPNAGLIVIDE
EHDGSYKQQDGIRYHARDFALVRGKALDVPVILGSATPSLESLHNAYAGRYQHLRLSQRAGEARPPRVRVLDVRKRPLKD
GLSPEVLAGIGTTLARGEQVLVFKNRRGYAPVLLCHDCGWTAACQRCSTPLHQTPMTVHAGGRRLQCHHCGARQPAPLAC
PACASLALQPQGIGTERLEERLTEAFPDVPVVRIDRSTTQRRDALETQLARLGTEAGILVGTQILAKGHDLPRLTMVVVV
GIDEGLFSADFRAAEKLAQQLIQVAGRAGRADRPGEVWLQTHHPEHPLLQTLVNGGYHAFADAELQQREAAGFPPFAHLA
LFRAEAKDVAAANQFLMAVRGLTTADSTAQSPAFAAVECYGPMPAPMPRRAGFQRSQLLLSAQQRSALHRLLDAQLPAIY
ALPQARRVRWSLDVDPIDLY
>Mature_739_residues
SSTVATLRVALPVPLPQLFDYLPPADAAPTDPARVGCRVRVPFGPRELVGVVVEIGQLPAADGLRPALAWCDQAPLLVDE
LARSLHWLARYTHAPLGEAQASALPGPLRRGEPLADTHAWAWQLTEAGRTGSTSLRAGSRPALLAALLRTGAVGEEQLDP
LLPQWREAARSLAKREYAERVAVPADTIPPRPGNGPPLNDEQQAATAAIRAHSGFATYLLDGVTGSGKTEVYLQAIADCL
AAGKQALVLVPEIGLTPQTLGRFRARLGVPVHALHSGLSDGERARVWAAAWRGQAQLIVGTRSAVFTPLPNAGLIVIDEE
HDGSYKQQDGIRYHARDFALVRGKALDVPVILGSATPSLESLHNAYAGRYQHLRLSQRAGEARPPRVRVLDVRKRPLKDG
LSPEVLAGIGTTLARGEQVLVFKNRRGYAPVLLCHDCGWTAACQRCSTPLHQTPMTVHAGGRRLQCHHCGARQPAPLACP
ACASLALQPQGIGTERLEERLTEAFPDVPVVRIDRSTTQRRDALETQLARLGTEAGILVGTQILAKGHDLPRLTMVVVVG
IDEGLFSADFRAAEKLAQQLIQVAGRAGRADRPGEVWLQTHHPEHPLLQTLVNGGYHAFADAELQQREAAGFPPFAHLAL
FRAEAKDVAAANQFLMAVRGLTTADSTAQSPAFAAVECYGPMPAPMPRRAGFQRSQLLLSAQQRSALHRLLDAQLPAIYA
LPQARRVRWSLDVDPIDLY

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=742, Percent_Identity=45.9568733153639, Blast_Score=607, Evalue=1e-174,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 79777; Mature: 79646

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTVATLRVALPVPLPQLFDYLPPADAAPTDPARVGCRVRVPFGPRELVGVVVEIGQLP
CCCCEEEEEEECCCCHHHHHHCCCCCCCCCCCHHHCCCEEECCCCHHHHHHHHHHHHCCC
AADGLRPALAWCDQAPLLVDELARSLHWLARYTHAPLGEAQASALPGPLRRGEPLADTHA
CCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCH
WAWQLTEAGRTGSTSLRAGSRPALLAALLRTGAVGEEQLDPLLPQWREAARSLAKREYAE
HEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHH
RVAVPADTIPPRPGNGPPLNDEQQAATAAIRAHSGFATYLLDGVTGSGKTEVYLQAIADC
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEHHEECCCCCCCHHHHHHHHHHH
LAAGKQALVLVPEIGLTPQTLGRFRARLGVPVHALHSGLSDGERARVWAAAWRGQAQLIV
HHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCHHEEEHHHHCCCEEEEE
GTRSAVFTPLPNAGLIVIDEEHDGSYKQQDGIRYHARDFALVRGKALDVPVILGSATPSL
ECCCEEECCCCCCCEEEEECCCCCCCCCCCCCEEECCCEEEECCCCCCCEEEECCCCCCH
ESLHNAYAGRYQHLRLSQRAGEARPPRVRVLDVRKRPLKDGLSPEVLAGIGTTLARGEQV
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHCCCCHHHHHHHHHHHHCCCEE
LVFKNRRGYAPVLLCHDCGWTAACQRCSTPLHQTPMTVHAGGRRLQCHHCGARQPAPLAC
EEEECCCCCCCEEEEECCCHHHHHHHHCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCC
PACASLALQPQGIGTERLEERLTEAFPDVPVVRIDRSTTQRRDALETQLARLGTEAGILV
HHHHHHEECCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCHHH
GTQILAKGHDLPRLTMVVVVGIDEGLFSADFRAAEKLAQQLIQVAGRAGRADRPGEVWLQ
HHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
THHPEHPLLQTLVNGGYHAFADAELQQREAAGFPPFAHLALFRAEAKDVAAANQFLMAVR
CCCCCCHHHHHHHCCCCEEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GLTTADSTAQSPAFAAVECYGPMPAPMPRRAGFQRSQLLLSAQQRSALHRLLDAQLPAIY
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
ALPQARRVRWSLDVDPIDLY
ECCCCCCEEEECCCCCCCCC
>Mature Secondary Structure 
SSTVATLRVALPVPLPQLFDYLPPADAAPTDPARVGCRVRVPFGPRELVGVVVEIGQLP
CCCEEEEEEECCCCHHHHHHCCCCCCCCCCCHHHCCCEEECCCCHHHHHHHHHHHHCCC
AADGLRPALAWCDQAPLLVDELARSLHWLARYTHAPLGEAQASALPGPLRRGEPLADTHA
CCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCH
WAWQLTEAGRTGSTSLRAGSRPALLAALLRTGAVGEEQLDPLLPQWREAARSLAKREYAE
HEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHH
RVAVPADTIPPRPGNGPPLNDEQQAATAAIRAHSGFATYLLDGVTGSGKTEVYLQAIADC
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEHHEECCCCCCCHHHHHHHHHHH
LAAGKQALVLVPEIGLTPQTLGRFRARLGVPVHALHSGLSDGERARVWAAAWRGQAQLIV
HHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCHHEEEHHHHCCCEEEEE
GTRSAVFTPLPNAGLIVIDEEHDGSYKQQDGIRYHARDFALVRGKALDVPVILGSATPSL
ECCCEEECCCCCCCEEEEECCCCCCCCCCCCCEEECCCEEEECCCCCCCEEEECCCCCCH
ESLHNAYAGRYQHLRLSQRAGEARPPRVRVLDVRKRPLKDGLSPEVLAGIGTTLARGEQV
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHCCCCHHHHHHHHHHHHCCCEE
LVFKNRRGYAPVLLCHDCGWTAACQRCSTPLHQTPMTVHAGGRRLQCHHCGARQPAPLAC
EEEECCCCCCCEEEEECCCHHHHHHHHCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCC
PACASLALQPQGIGTERLEERLTEAFPDVPVVRIDRSTTQRRDALETQLARLGTEAGILV
HHHHHHEECCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCHHH
GTQILAKGHDLPRLTMVVVVGIDEGLFSADFRAAEKLAQQLIQVAGRAGRADRPGEVWLQ
HHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
THHPEHPLLQTLVNGGYHAFADAELQQREAAGFPPFAHLALFRAEAKDVAAANQFLMAVR
CCCCCCHHHHHHHCCCCEEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GLTTADSTAQSPAFAAVECYGPMPAPMPRRAGFQRSQLLLSAQQRSALHRLLDAQLPAIY
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
ALPQARRVRWSLDVDPIDLY
ECCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]