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The map label for this gene is holC [H]

Identifier: 21244283

GI number: 21244283

Start: 4216987

End: 4217412

Strand: Direct

Name: holC [H]

Synonym: XAC3558

Alternate gene names: 21244283

Gene position: 4216987-4217412 (Clockwise)

Preceding gene: 21244282

Following gene: 21244284

Centisome position: 81.48

GC content: 67.14

Gene sequence:

>426_bases
ATGCCCCGTGCCGATTTTTATCTGATCGCCAAGCCGCGCTTCCTCAACGAACCGCTGCGGCTGGTCTGCGAGCTGGCGCG
CAAGGCCAACGACGCCAACCTGTCCACGCTGATCCTGGCCCGCGATGCGGAGCAGGCCGAAGCGCTGGACGATCTGCTGT
GGGCGTTCGACGACGAGGCCTACGTGCCGCACCAGATCGCCGGCACCGACGAGGAAGACGAACTGGCACCGGTGCTGATC
GCCGCACCGGAATTCGCCGCCCCCTCGCGCCCGCTGGTGATCAACCTGCGCGACGACCCGTACCTGGGCGCCTGCGACCG
CGTGCTCGAAGTCGTCCCCGCCGACCCCGCTGCCCGCGAACCGCTGCGCGAACGCTGGAAACAATACAAGGCCCTGGGCC
TGGAACTGACCAAGTACGACATGTGA

Upstream 100 bases:

>100_bases
ATGGGCGGGTTCGTGCTGGTGATCCTGGCGTCCGGCCTGCTGTTCACGCTCGGCAGCATCATTGCCGAATCCGTTTTTCC
TTCCGTTTCGTGATCTGACC

Downstream 100 bases:

>100_bases
ATCGGGAATGGGGAATGGGGAATCGAAACTTGGCTCCGTCCCATTAGACCGTCACCGATCAGCACCTCCCGCTTTACCCA
TTCCCTATTCCCTATTCCCT

Product: DNA polymerase III subunit chi

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 141; Mature: 140

Protein sequence:

>141_residues
MPRADFYLIAKPRFLNEPLRLVCELARKANDANLSTLILARDAEQAEALDDLLWAFDDEAYVPHQIAGTDEEDELAPVLI
AAPEFAAPSRPLVINLRDDPYLGACDRVLEVVPADPAAREPLRERWKQYKALGLELTKYDM

Sequences:

>Translated_141_residues
MPRADFYLIAKPRFLNEPLRLVCELARKANDANLSTLILARDAEQAEALDDLLWAFDDEAYVPHQIAGTDEEDELAPVLI
AAPEFAAPSRPLVINLRDDPYLGACDRVLEVVPADPAAREPLRERWKQYKALGLELTKYDM
>Mature_140_residues
PRADFYLIAKPRFLNEPLRLVCELARKANDANLSTLILARDAEQAEALDDLLWAFDDEAYVPHQIAGTDEEDELAPVLIA
APEFAAPSRPLVINLRDDPYLGACDRVLEVVPADPAAREPLRERWKQYKALGLELTKYDM

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG2927

COG function: function code L; DNA polymerase III, chi subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790709, Length=143, Percent_Identity=34.2657342657343, Blast_Score=65, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007459 [H]

Pfam domain/function: PF04364 DNA_pol3_chi [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 15904; Mature: 15773

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRADFYLIAKPRFLNEPLRLVCELARKANDANLSTLILARDAEQAEALDDLLWAFDDEA
CCCCCEEEEECCHHHCHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCC
YVPHQIAGTDEEDELAPVLIAAPEFAAPSRPLVINLRDDPYLGACDRVLEVVPADPAARE
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCH
PLRERWKQYKALGLELTKYDM
HHHHHHHHHHHHCCEEEECCC
>Mature Secondary Structure 
PRADFYLIAKPRFLNEPLRLVCELARKANDANLSTLILARDAEQAEALDDLLWAFDDEA
CCCCEEEEECCHHHCHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCC
YVPHQIAGTDEEDELAPVLIAAPEFAAPSRPLVINLRDDPYLGACDRVLEVVPADPAARE
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCH
PLRERWKQYKALGLELTKYDM
HHHHHHHHHHHHCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9864319; 10984043 [H]