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The map label for this gene is cit1 [H]

Identifier: 21244199

GI number: 21244199

Start: 4107105

End: 4108454

Strand: Direct

Name: cit1 [H]

Synonym: XAC3474

Alternate gene names: 21244199

Gene position: 4107105-4108454 (Clockwise)

Preceding gene: 21244198

Following gene: 21244200

Centisome position: 79.36

GC content: 63.41

Gene sequence:

>1350_bases
ATGCATACCTATCGTGGAGGGCGATTTATGCAGAGTTCCAGCAGTGAGGCAACGCGCCAGTCGAAACTCGGGATGGTGCT
GAGGGTGACCTCGGGCAACTTCCTCGAGCAGTTCGACTTCTTCCTGTTCGGCTTTTACGCAACGCAGATCGCCGCGACCT
TCTTCCCTGCGAGCAACGAATTCGCGTCCCTGATGATGACCTTCGCCGTGTTTGGCGCGGGCTTTCTGATGCGGCCGCTG
GGTGCGGTGATCCTGGGTGCCTACATCGACGATGTCGGGCGCCGCAAGGGCTTGATCGTCACCTTGGCGATCATGGCCAG
CGGTACGGTGCTGATCGTGCTGGTGCCAGGCTACGCCAGCATCGGCTTGTGGGCACCGGCCCTGGTGCTGCTTGGCCGCC
TGCTGCAGGGCTTTTCGGCCGGCGCCGAAATGGGCGGCGTGTCGGTGTATCTGGCCGAGATGGCCACACCCGGTCGGCGC
GGCTTCTACGCCAGTTGGCAATCGGCCAGCCAGCAGCTTGCCATCGTCGCTGCGGCCGCAATCGGGTATGCGCTCAACCA
GCTGATGGCGCCGCAGGACCTGGCGCAGTGGGGCTGGCGAATTCCGTTCGGCATCGGCTGCGTCATCATTCCTTTCATCT
TCTTGCTGCGGCGAAGCCTGGAAGAGACTGCAGAATTTGCCCAGCGCCGGCAGCCGGTCACGATGAAGCAAGTGATGCGC
GGGCTTGCGAACAATGCCGGCATCGTGATCGCCGGCGGCTTGATGGTCGCGCTGACGACGACCGCGTTCTATCTGATCAC
CGTCTACGCCCCCACCTTCGGCAAGAGCGTACTCAAACTCAGCACCGGCGATGCGTTGATCGTCACGCTGTTGGTAGGCG
TCTCCAATTTCATCTGGTTGCCGATCGGTGGCGCGCTCAGCGACCGCTTCGGCCGCAAGCCGCTGCTGGTGAGCATGGCG
GTGGTATGCATGGTAACGGCCTATCCGGTGCTTGCGTTTCTCGCGGCCGCACCGAGCTTTACGCATATGTTGCAGGCGCT
GCTGTGGCTGTCCTTCATCTACGGCCTCTACAACGGCGCCATGATTCCGGCGCTCACCGAACTCATGCCGGCGCATGTGC
GGGTCGCCGGATTCTCGCTTGCCTACAGCCTGGCGACCGCGGTGTTTGGCGGCTTTACGCCGGTGATGTCCACCTGGCTG
ATCCATGTCACCGGCGACAAGGCCGCGCCTGGCTACTGGCTGGTCTTCGCGTCGGCGTGCGCGCTGGGGGCCACGCTGTT
TCTGTATCGCCGCCAGAGCAATCCCGCGACGGCGACCGGCCTGGATACCGCGGCAGGAGCGACGAAATGA

Upstream 100 bases:

>100_bases
GCGCGGACCGATGTTCAACGCGCATCAATCGATGAGAAAGCAGCAATTATCAACGTCGACCATCTCGACCAGAATCGAGT
GCCTCAGATGTCATACGTCC

Downstream 100 bases:

>100_bases
TGCGGACCATGTGGAAAAAGTGGCTGGGTGCGATCGTTGCAACGGCTCTGCTGTCGACAGCGGCAGGCGCGCAGGAATTG
ACCGTGATGACCTCCGGCGG

Product: citrate carrier protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MHTYRGGRFMQSSSSEATRQSKLGMVLRVTSGNFLEQFDFFLFGFYATQIAATFFPASNEFASLMMTFAVFGAGFLMRPL
GAVILGAYIDDVGRRKGLIVTLAIMASGTVLIVLVPGYASIGLWAPALVLLGRLLQGFSAGAEMGGVSVYLAEMATPGRR
GFYASWQSASQQLAIVAAAAIGYALNQLMAPQDLAQWGWRIPFGIGCVIIPFIFLLRRSLEETAEFAQRRQPVTMKQVMR
GLANNAGIVIAGGLMVALTTTAFYLITVYAPTFGKSVLKLSTGDALIVTLLVGVSNFIWLPIGGALSDRFGRKPLLVSMA
VVCMVTAYPVLAFLAAAPSFTHMLQALLWLSFIYGLYNGAMIPALTELMPAHVRVAGFSLAYSLATAVFGGFTPVMSTWL
IHVTGDKAAPGYWLVFASACALGATLFLYRRQSNPATATGLDTAAGATK

Sequences:

>Translated_449_residues
MHTYRGGRFMQSSSSEATRQSKLGMVLRVTSGNFLEQFDFFLFGFYATQIAATFFPASNEFASLMMTFAVFGAGFLMRPL
GAVILGAYIDDVGRRKGLIVTLAIMASGTVLIVLVPGYASIGLWAPALVLLGRLLQGFSAGAEMGGVSVYLAEMATPGRR
GFYASWQSASQQLAIVAAAAIGYALNQLMAPQDLAQWGWRIPFGIGCVIIPFIFLLRRSLEETAEFAQRRQPVTMKQVMR
GLANNAGIVIAGGLMVALTTTAFYLITVYAPTFGKSVLKLSTGDALIVTLLVGVSNFIWLPIGGALSDRFGRKPLLVSMA
VVCMVTAYPVLAFLAAAPSFTHMLQALLWLSFIYGLYNGAMIPALTELMPAHVRVAGFSLAYSLATAVFGGFTPVMSTWL
IHVTGDKAAPGYWLVFASACALGATLFLYRRQSNPATATGLDTAAGATK
>Mature_449_residues
MHTYRGGRFMQSSSSEATRQSKLGMVLRVTSGNFLEQFDFFLFGFYATQIAATFFPASNEFASLMMTFAVFGAGFLMRPL
GAVILGAYIDDVGRRKGLIVTLAIMASGTVLIVLVPGYASIGLWAPALVLLGRLLQGFSAGAEMGGVSVYLAEMATPGRR
GFYASWQSASQQLAIVAAAAIGYALNQLMAPQDLAQWGWRIPFGIGCVIIPFIFLLRRSLEETAEFAQRRQPVTMKQVMR
GLANNAGIVIAGGLMVALTTTAFYLITVYAPTFGKSVLKLSTGDALIVTLLVGVSNFIWLPIGGALSDRFGRKPLLVSMA
VVCMVTAYPVLAFLAAAPSFTHMLQALLWLSFIYGLYNGAMIPALTELMPAHVRVAGFSLAYSLATAVFGGFTPVMSTWL
IHVTGDKAAPGYWLVFASACALGATLFLYRRQSNPATATGLDTAAGATK

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=410, Percent_Identity=30.7317073170732, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1788942, Length=427, Percent_Identity=33.0210772833724, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1788292, Length=415, Percent_Identity=29.1566265060241, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1789941, Length=387, Percent_Identity=29.1989664082687, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI87082231, Length=224, Percent_Identity=27.2321428571429, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47963; Mature: 47963

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTYRGGRFMQSSSSEATRQSKLGMVLRVTSGNFLEQFDFFLFGFYATQIAATFFPASNE
CCCCCCCCEECCCCHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
FASLMMTFAVFGAGFLMRPLGAVILGAYIDDVGRRKGLIVTLAIMASGTVLIVLVPGYAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHCCCEEEEEECCCHH
IGLWAPALVLLGRLLQGFSAGAEMGGVSVYLAEMATPGRRGFYASWQSASQQLAIVAAAA
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHHH
IGYALNQLMAPQDLAQWGWRIPFGIGCVIIPFIFLLRRSLEETAEFAQRRQPVTMKQVMR
HHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GLANNAGIVIAGGLMVALTTTAFYLITVYAPTFGKSVLKLSTGDALIVTLLVGVSNFIWL
HHCCCCCEEEECHHHHHHHHHHHHHHHEECCCCCCHHEEECCCCHHHHHHHHHHHHEEEE
PIGGALSDRFGRKPLLVSMAVVCMVTAYPVLAFLAAAPSFTHMLQALLWLSFIYGLYNGA
ECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCH
MIPALTELMPAHVRVAGFSLAYSLATAVFGGFTPVMSTWLIHVTGDKAAPGYWLVFASAC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEEEEECCCCCCCHHHHHHHHH
ALGATLFLYRRQSNPATATGLDTAAGATK
HHHHHHHEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHTYRGGRFMQSSSSEATRQSKLGMVLRVTSGNFLEQFDFFLFGFYATQIAATFFPASNE
CCCCCCCCEECCCCHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
FASLMMTFAVFGAGFLMRPLGAVILGAYIDDVGRRKGLIVTLAIMASGTVLIVLVPGYAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHCCCEEEEEECCCHH
IGLWAPALVLLGRLLQGFSAGAEMGGVSVYLAEMATPGRRGFYASWQSASQQLAIVAAAA
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHHH
IGYALNQLMAPQDLAQWGWRIPFGIGCVIIPFIFLLRRSLEETAEFAQRRQPVTMKQVMR
HHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GLANNAGIVIAGGLMVALTTTAFYLITVYAPTFGKSVLKLSTGDALIVTLLVGVSNFIWL
HHCCCCCEEEECHHHHHHHHHHHHHHHEECCCCCCHHEEECCCCHHHHHHHHHHHHEEEE
PIGGALSDRFGRKPLLVSMAVVCMVTAYPVLAFLAAAPSFTHMLQALLWLSFIYGLYNGA
ECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCH
MIPALTELMPAHVRVAGFSLAYSLATAVFGGFTPVMSTWLIHVTGDKAAPGYWLVFASAC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEEEEECCCCCCCHHHHHHHHH
ALGATLFLYRRQSNPATATGLDTAAGATK
HHHHHHHEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]