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The map label for this gene is pfkA [H]

Identifier: 21244163

GI number: 21244163

Start: 4048222

End: 4049478

Strand: Direct

Name: pfkA [H]

Synonym: XAC3438

Alternate gene names: 21244163

Gene position: 4048222-4049478 (Clockwise)

Preceding gene: 21244158

Following gene: 21244165

Centisome position: 78.22

GC content: 65.08

Gene sequence:

>1257_bases
ATGACCACTGGCAACCTGCTCTATGCCCAATCCGGCGGCGTCACCGCCGTCATCAATGCCACTGCCGCCGGCGTCATCGC
CGAAGCGCGCGCACGCAAGATCAAGGTACTGGCTGCGCGCAATGGCATCCTGGGCGCCTTGCGCGAGGAGTTGATCGATA
CCTCCAAGGAATCCGCTGCAGCCATTGCCGCATTGGCGCAGACCCCCGGCGGCGCTTTCGGCTCGTGCCGCTACAAGCTC
AAATCGCTGGAGGAAGACAGCGCCAAGTACGAACGTCTGCTCGACGTGCTGCGCGCGCACGACGTGCGCTGGTTCCTCTA
CAACGGCGGCAACGACTCGGCCGACACCGCGTGGAAGGTCTCGCAACTCGCAAAGGCCTATGGTTACCCGCTGCACTGCA
TCGGCGTGCCAAAGACCATCGACAACGATCTGGCGGCGACCGACACCTGCCCTGGCTTCGGCTCGGCGGCCAAGTACACC
GTCGTCTCGGTACGCGAGGCCGCACTGGATGTCGCCGCCATGGCCGACACCTCCACCAAGGTCTTCATCTATGAGGCGAT
GGGCCGCCATGCCGGTTGGCTGGCCGCAGCGGCAGGCCTGGCCGGGCAAGGCCCGGACGACGCCCCGCAGATCATCCTGC
TACCCGAACGCGCCTACGACCAGGCTGCATTCCTGGCCAAGGTGAAACAGGTGGTGGAGAAGGTCGGCTGGTGCGTGGTG
GTGGCCAGCGAGGGCATCCAGGACGCACACGGCAAGTTCGTCGCCGATGCCGGTGGCGCGGCCGACTCGTTCGGCCATGC
ACAGCTCGGCGGCGTGGCCTCGTTCCTGGCCGCACAGGTCAAGCAGGAACTCGGCTACAAGGTGCACTGGACGTTGCCGG
ATTACCTGCAGCGCTCCGCACGCCACCTCGCATCCAAGACGGACTGGGAACAGGCGCAAGCGGTCGGCAAGGCCGCCGTG
CAATACGCGATCAAAGGCATGAACGCGGTGATCCCGGTGATCGAACGCGTCAGCGACGCACCCTACCGCTGGAAGATCGT
GCCCGCCCCCCTGCACAAGGTGGCCAATCACGAAAAGAAGATGCCGCCCAGCTTCCTGCGCAAGGACGGCTTCGGCATCA
CCGAACGCGCCCGCCGCTACTTCGCCCCGTTGATCAAGGGCGAAGCCGCGCTGGCCTACGGCAGCGATGGCTTGCCGAAG
TATGTGAGTTTGAAGAATGTCGCGGTGGCGAAGAAATTGCCAGCTTGGGAGGGGTGA

Upstream 100 bases:

>100_bases
CGCCGGCGCTGACCGTATGGCCGTGCAGCTTACCGCATCCGGGGAATGTCCCGGCACTGTCCCCCTGCGCCATGTTCCCA
TGTGAGGAGTCTGTCCCCCT

Downstream 100 bases:

>100_bases
GATCGGCGCCGCGTGGGATGCACAGCGATGTTCAACTCTCCGCTGAGGCGGCGGCACATCGGGCGACTCATGCGCGTGGG
TTGCGCCCTAACGAAAGGTG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: ATP-PFK; Phosphofructokinase 1; Phosphohexokinase 1 [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MTTGNLLYAQSGGVTAVINATAAGVIAEARARKIKVLAARNGILGALREELIDTSKESAAAIAALAQTPGGAFGSCRYKL
KSLEEDSAKYERLLDVLRAHDVRWFLYNGGNDSADTAWKVSQLAKAYGYPLHCIGVPKTIDNDLAATDTCPGFGSAAKYT
VVSVREAALDVAAMADTSTKVFIYEAMGRHAGWLAAAAGLAGQGPDDAPQIILLPERAYDQAAFLAKVKQVVEKVGWCVV
VASEGIQDAHGKFVADAGGAADSFGHAQLGGVASFLAAQVKQELGYKVHWTLPDYLQRSARHLASKTDWEQAQAVGKAAV
QYAIKGMNAVIPVIERVSDAPYRWKIVPAPLHKVANHEKKMPPSFLRKDGFGITERARRYFAPLIKGEAALAYGSDGLPK
YVSLKNVAVAKKLPAWEG

Sequences:

>Translated_418_residues
MTTGNLLYAQSGGVTAVINATAAGVIAEARARKIKVLAARNGILGALREELIDTSKESAAAIAALAQTPGGAFGSCRYKL
KSLEEDSAKYERLLDVLRAHDVRWFLYNGGNDSADTAWKVSQLAKAYGYPLHCIGVPKTIDNDLAATDTCPGFGSAAKYT
VVSVREAALDVAAMADTSTKVFIYEAMGRHAGWLAAAAGLAGQGPDDAPQIILLPERAYDQAAFLAKVKQVVEKVGWCVV
VASEGIQDAHGKFVADAGGAADSFGHAQLGGVASFLAAQVKQELGYKVHWTLPDYLQRSARHLASKTDWEQAQAVGKAAV
QYAIKGMNAVIPVIERVSDAPYRWKIVPAPLHKVANHEKKMPPSFLRKDGFGITERARRYFAPLIKGEAALAYGSDGLPK
YVSLKNVAVAKKLPAWEG
>Mature_417_residues
TTGNLLYAQSGGVTAVINATAAGVIAEARARKIKVLAARNGILGALREELIDTSKESAAAIAALAQTPGGAFGSCRYKLK
SLEEDSAKYERLLDVLRAHDVRWFLYNGGNDSADTAWKVSQLAKAYGYPLHCIGVPKTIDNDLAATDTCPGFGSAAKYTV
VSVREAALDVAAMADTSTKVFIYEAMGRHAGWLAAAAGLAGQGPDDAPQIILLPERAYDQAAFLAKVKQVVEKVGWCVVV
ASEGIQDAHGKFVADAGGAADSFGHAQLGGVASFLAAQVKQELGYKVHWTLPDYLQRSARHLASKTDWEQAQAVGKAAVQ
YAIKGMNAVIPVIERVSDAPYRWKIVPAPLHKVANHEKKMPPSFLRKDGFGITERARRYFAPLIKGEAALAYGSDGLPKY
VSLKNVAVAKKLPAWEG

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=271, Percent_Identity=27.3062730627306, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 44536; Mature: 44405

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTGNLLYAQSGGVTAVINATAAGVIAEARARKIKVLAARNGILGALREELIDTSKESAA
CCCCCEEEEECCCEEEEEECHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHCCCHHHHH
AIAALAQTPGGAFGSCRYKLKSLEEDSAKYERLLDVLRAHDVRWFLYNGGNDSADTAWKV
HHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHH
SQLAKAYGYPLHCIGVPKTIDNDLAATDTCPGFGSAAKYTVVSVREAALDVAAMADTSTK
HHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCE
VFIYEAMGRHAGWLAAAAGLAGQGPDDAPQIILLPERAYDQAAFLAKVKQVVEKVGWCVV
EEEEECCCCCCHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCEEEE
VASEGIQDAHGKFVADAGGAADSFGHAQLGGVASFLAAQVKQELGYKVHWTLPDYLQRSA
EECCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHH
RHLASKTDWEQAQAVGKAAVQYAIKGMNAVIPVIERVSDAPYRWKIVPAPLHKVANHEKK
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCCCC
MPPSFLRKDGFGITERARRYFAPLIKGEAALAYGSDGLPKYVSLKNVAVAKKLPAWEG
CCHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TTGNLLYAQSGGVTAVINATAAGVIAEARARKIKVLAARNGILGALREELIDTSKESAA
CCCCEEEEECCCEEEEEECHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHCCCHHHHH
AIAALAQTPGGAFGSCRYKLKSLEEDSAKYERLLDVLRAHDVRWFLYNGGNDSADTAWKV
HHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHH
SQLAKAYGYPLHCIGVPKTIDNDLAATDTCPGFGSAAKYTVVSVREAALDVAAMADTSTK
HHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCE
VFIYEAMGRHAGWLAAAAGLAGQGPDDAPQIILLPERAYDQAAFLAKVKQVVEKVGWCVV
EEEEECCCCCCHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCEEEE
VASEGIQDAHGKFVADAGGAADSFGHAQLGGVASFLAAQVKQELGYKVHWTLPDYLQRSA
EECCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHH
RHLASKTDWEQAQAVGKAAVQYAIKGMNAVIPVIERVSDAPYRWKIVPAPLHKVANHEKK
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCCCC
MPPSFLRKDGFGITERARRYFAPLIKGEAALAYGSDGLPKYVSLKNVAVAKKLPAWEG
CCHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9055413; 12000953 [H]