Click here to switch to the map view.

The map label for this gene is rph

Identifier: 21244122

GI number: 21244122

Start: 4004369

End: 4005094

Strand: Direct

Name: rph

Synonym: XAC3397

Alternate gene names: 21244122

Gene position: 4004369-4005094 (Clockwise)

Preceding gene: 21244112

Following gene: 21244123

Centisome position: 77.37

GC content: 68.18

Gene sequence:

>726_bases
ATGACCTTTTCCCGTCCCAGCGGCCGCACGGCCGACCAGCTGCGCCCGGTGCGCATCGAACGCTCCTTCACCCGCCATGC
CGAAGGCTCGGTGCTGGTCAGCTTCGGCGACACCCGCGTGCTGTGCACCGCCAGCGTGGAAAACCGCGTGCCGGGTTTCC
TGCGCGGCAAGGGCGAAGGCTGGGTCACCGCCGAATACGGCATGTTGCCGCGCTCCACCCACACCCGTTCCGACCGCGAG
GCCGCGCGCGGCAAGCAGGGCGGCCGCACGCTGGAAATCCAGCGTCTGATCGGCCGCGCGCTGCGCGCCTGCGTGGACCG
CAATGCACTTGGCGAGCGCACCATCACCCTGGACTGCGACGTGCTGCAGGCCGATGGCGGCACCCGCACCGCGGCCATCA
CCGGCGCGTACGTGGCGCTGGCCGATGCGGTGAACCTGCTGATCAAGCGCGGCGACATCAAGAAGCACCCGCTGATCGGC
GCGGTGGCTGCGGTGTCGGTCGGCATCTATCGTGGTGAGCCGGTGCTGGATCTGGATTACCCGGAAGACAGCGACTGCGA
TACCGACATGAATGTGGTGATGAACGACGGCGGCGGCTTCATCGAACTGCAGGGCACGGCCGAAGGCCACGCCTTCCGTC
GCGACGAACTCAACGCGCTGCTCGCCCTGGCCGAAAAAGGCATGGGCGAGTTGTTCGCACTGCAACGCGCTGCGCTGGCC
GGATGA

Upstream 100 bases:

>100_bases
CGTGCCGTCAAAGCGCGAATGGTACCCTAGCCGCCCTCGCCGGCTGCCTGCACGCTGCGCAGTGGCGCCAGACCCACCTC
CCGACTTCCGGACTCATCGC

Downstream 100 bases:

>100_bases
GCCGTCGTATCGCCCTGACCACCTTGCTGGTTGCCGATTACGATGCGGCCATTGCCTGGTACACCGGCGCGCTGGGTTTC
GGCGTGCTGCAGGATCGCCC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MTFSRPSGRTADQLRPVRIERSFTRHAEGSVLVSFGDTRVLCTASVENRVPGFLRGKGEGWVTAEYGMLPRSTHTRSDRE
AARGKQGGRTLEIQRLIGRALRACVDRNALGERTITLDCDVLQADGGTRTAAITGAYVALADAVNLLIKRGDIKKHPLIG
AVAAVSVGIYRGEPVLDLDYPEDSDCDTDMNVVMNDGGGFIELQGTAEGHAFRRDELNALLALAEKGMGELFALQRAALA
G

Sequences:

>Translated_241_residues
MTFSRPSGRTADQLRPVRIERSFTRHAEGSVLVSFGDTRVLCTASVENRVPGFLRGKGEGWVTAEYGMLPRSTHTRSDRE
AARGKQGGRTLEIQRLIGRALRACVDRNALGERTITLDCDVLQADGGTRTAAITGAYVALADAVNLLIKRGDIKKHPLIG
AVAAVSVGIYRGEPVLDLDYPEDSDCDTDMNVVMNDGGGFIELQGTAEGHAFRRDELNALLALAEKGMGELFALQRAALA
G
>Mature_240_residues
TFSRPSGRTADQLRPVRIERSFTRHAEGSVLVSFGDTRVLCTASVENRVPGFLRGKGEGWVTAEYGMLPRSTHTRSDREA
ARGKQGGRTLEIQRLIGRALRACVDRNALGERTITLDCDVLQADGGTRTAAITGAYVALADAVNLLIKRGDIKKHPLIGA
VAAVSVGIYRGEPVLDLDYPEDSDCDTDMNVVMNDGGGFIELQGTAEGHAFRRDELNALLALAEKGMGELFALQRAALAG

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=208, Percent_Identity=69.7115384615385, Blast_Score=301, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI71981632, Length=186, Percent_Identity=26.8817204301075, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_XANAC (Q8PH63)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643704.1
- ProteinModelPortal:   Q8PH63
- SMR:   Q8PH63
- GeneID:   1157468
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3397
- NMPDR:   fig|190486.1.peg.3348
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- BioCyc:   XAXO190486:XAC3397-MONOMER
- BRENDA:   2.7.7.56
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25927; Mature: 25796

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSRPSGRTADQLRPVRIERSFTRHAEGSVLVSFGDTRVLCTASVENRVPGFLRGKGEG
CCCCCCCCCCHHHCCCEEEEHHHHHCCCCCEEEEECCCEEEEEECCCCCCCCEEEECCCC
WVTAEYGMLPRSTHTRSDREAARGKQGGRTLEIQRLIGRALRACVDRNALGERTITLDCD
EEEEECCCCCCCCCCCCHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
VLQADGGTRTAAITGAYVALADAVNLLIKRGDIKKHPLIGAVAAVSVGIYRGEPVLDLDY
EEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEEECCCEEEECCC
PEDSDCDTDMNVVMNDGGGFIELQGTAEGHAFRRDELNALLALAEKGMGELFALQRAALA
CCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHC
G
C
>Mature Secondary Structure 
TFSRPSGRTADQLRPVRIERSFTRHAEGSVLVSFGDTRVLCTASVENRVPGFLRGKGEG
CCCCCCCCCHHHCCCEEEEHHHHHCCCCCEEEEECCCEEEEEECCCCCCCCEEEECCCC
WVTAEYGMLPRSTHTRSDREAARGKQGGRTLEIQRLIGRALRACVDRNALGERTITLDCD
EEEEECCCCCCCCCCCCHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
VLQADGGTRTAAITGAYVALADAVNLLIKRGDIKKHPLIGAVAAVSVGIYRGEPVLDLDY
EEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEEECCCEEEECCC
PEDSDCDTDMNVVMNDGGGFIELQGTAEGHAFRRDELNALLALAEKGMGELFALQRAALA
CCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217