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The map label for this gene is mltB [H]

Identifier: 21243951

GI number: 21243951

Start: 3798986

End: 3800263

Strand: Reverse

Name: mltB [H]

Synonym: XAC3225

Alternate gene names: 21243951

Gene position: 3800263-3798986 (Counterclockwise)

Preceding gene: 21243952

Following gene: 21243950

Centisome position: 73.43

GC content: 62.52

Gene sequence:

>1278_bases
ATGATCATGCCTTCGCGCCTTTTGCGTTTAACGCTCGGTGTCAGCGTCTGTGTGGCCGCAACCAGCGTAGCAGCGCAAGC
CATCGCTCCCGAAACAACCGCATCCTCCGCAACCGACGCCGGTGGGCAACAGCAGGATCCCGCACCCACTGCGAGCTTCG
AACAGTGGCTGGCAGACTTCCGCCAACGCGCGCTTGCCGCCGGCATTGGAGCCACCACGCTGGATAACGCATTGGCTGGT
GTCACACCCGATCCAGCGGTGCATGAACTGGATCAGCGGCAACCCGAATTCACCCAGTATCTATGGGACTATCTCGACGC
GCGTGTCACACCTTCGGCCATCCAGGAAGGACAGCAGCTGCTGATCAGCCAGCACGCCCTCTTCGAGAAGTTGCGGCAGC
ACTACGGTGTGGATCCGGGCATTCTGACCGCCATCTGGAGCATGGAAAGCGGGTACGGCAAGCAGATCGGGGACTTTTAT
GTCATTCGCTCCCTGGCGACACTGGCCCATGAGGGACGGCGCACCACGTATGGCAACACGCAGCTGCTTGCGGCCCTGCA
GATCCTGCAGACGGAAAAAAGCATTGATCGCTCGCAACTGGTGGGTTCGTGGGCCGGCGCCATGGGACAGACGCAATTCA
TTCCGAGCACCTATCGCGACTACGCCGTCGACGAGGATGGCGACCAGAAGCGGGATGTCTGGAATTCCAAGGCAGACGCT
TTGGGCTCTGCGGCGAATTATCTCAAGCAGAACAATTGGACCAGCGCTGTTCCCTGGGGCCAGGAAGTCCAGCTGTCAGC
CGGCTTCGACTACGCGCAGGCCGACCTGACGATCAAGAAGACCGTCGCCGAATGGCAGCGCCTGGGCGTGGCGCCCCGCA
GGCCGATTGCGCCCGCACTGGCGCAGCAGTTGGCATCGGTGTTGTTGCCCACCGGATACCGCGGCCCGGCCTTCCTAGTG
TTCGATAACTTCCGCAGCATTCTGCGCTACAACAATTCCACCGCCTATGCCCTGGCGGTCGGCTTGCTGGCAGACGGCTA
TGCGGGCAGGGCCGGGGTCAAGCAACCCTGGCCCAAAGACGATCCGCCGCTCAATAGCACGGCCCAGATCACCGAGCTGC
AGCAGAGGCTGACCGACAAGGGATTCGACGTGGGCGGCATCGACGGTGTGCTGGGGGCGCAAACTCGCCAGGGAATCCGT
GCATTCCAGCGTAGCCAGCAATTGCCGCAGGACGGTTACGCCAGTACGTCGCTGCTGGCCCGTCTGCGGGCCGCCTGA

Upstream 100 bases:

>100_bases
TCCTGCTTGCCCATCGCGGTCAAAAACGCATAGCTGCAATAATTACATCCTGGGAACAGGAGGACTAGAGTGGCCGCTCA
ACCACCACCTCGGGTATCTC

Downstream 100 bases:

>100_bases
TCGGGGATGTACGTCGGAGGCTGCAATTCTTCACCCACTTACAAGTACTGCAAGGCCATCAATAATGGTGCGGGAATCTC
AAAAATGGGTTGCACTATCT

Product: transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MIMPSRLLRLTLGVSVCVAATSVAAQAIAPETTASSATDAGGQQQDPAPTASFEQWLADFRQRALAAGIGATTLDNALAG
VTPDPAVHELDQRQPEFTQYLWDYLDARVTPSAIQEGQQLLISQHALFEKLRQHYGVDPGILTAIWSMESGYGKQIGDFY
VIRSLATLAHEGRRTTYGNTQLLAALQILQTEKSIDRSQLVGSWAGAMGQTQFIPSTYRDYAVDEDGDQKRDVWNSKADA
LGSAANYLKQNNWTSAVPWGQEVQLSAGFDYAQADLTIKKTVAEWQRLGVAPRRPIAPALAQQLASVLLPTGYRGPAFLV
FDNFRSILRYNNSTAYALAVGLLADGYAGRAGVKQPWPKDDPPLNSTAQITELQQRLTDKGFDVGGIDGVLGAQTRQGIR
AFQRSQQLPQDGYASTSLLARLRAA

Sequences:

>Translated_425_residues
MIMPSRLLRLTLGVSVCVAATSVAAQAIAPETTASSATDAGGQQQDPAPTASFEQWLADFRQRALAAGIGATTLDNALAG
VTPDPAVHELDQRQPEFTQYLWDYLDARVTPSAIQEGQQLLISQHALFEKLRQHYGVDPGILTAIWSMESGYGKQIGDFY
VIRSLATLAHEGRRTTYGNTQLLAALQILQTEKSIDRSQLVGSWAGAMGQTQFIPSTYRDYAVDEDGDQKRDVWNSKADA
LGSAANYLKQNNWTSAVPWGQEVQLSAGFDYAQADLTIKKTVAEWQRLGVAPRRPIAPALAQQLASVLLPTGYRGPAFLV
FDNFRSILRYNNSTAYALAVGLLADGYAGRAGVKQPWPKDDPPLNSTAQITELQQRLTDKGFDVGGIDGVLGAQTRQGIR
AFQRSQQLPQDGYASTSLLARLRAA
>Mature_425_residues
MIMPSRLLRLTLGVSVCVAATSVAAQAIAPETTASSATDAGGQQQDPAPTASFEQWLADFRQRALAAGIGATTLDNALAG
VTPDPAVHELDQRQPEFTQYLWDYLDARVTPSAIQEGQQLLISQHALFEKLRQHYGVDPGILTAIWSMESGYGKQIGDFY
VIRSLATLAHEGRRTTYGNTQLLAALQILQTEKSIDRSQLVGSWAGAMGQTQFIPSTYRDYAVDEDGDQKRDVWNSKADA
LGSAANYLKQNNWTSAVPWGQEVQLSAGFDYAQADLTIKKTVAEWQRLGVAPRRPIAPALAQQLASVLLPTGYRGPAFLV
FDNFRSILRYNNSTAYALAVGLLADGYAGRAGVKQPWPKDDPPLNSTAQITELQQRLTDKGFDVGGIDGVLGAQTRQGIR
AFQRSQQLPQDGYASTSLLARLRAA

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=243, Percent_Identity=32.9218106995885, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 46144; Mature: 46144

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCC
>Mature Secondary Structure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HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]