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The map label for this gene is aspS

Identifier: 21243880

GI number: 21243880

Start: 3704027

End: 3705793

Strand: Reverse

Name: aspS

Synonym: XAC3154

Alternate gene names: 21243880

Gene position: 3705793-3704027 (Counterclockwise)

Preceding gene: 21243882

Following gene: 77748701

Centisome position: 71.6

GC content: 65.87

Gene sequence:

>1767_bases
ATGCGTACCCACTTCTGCGGCCTGGTCGACGAGACCTTGATCGGCCAAACCGTCACTCTCGCCGGCTGGACCGACGTGGC
CCGCAACCTGGGCGGGGTGTGCTTCATCGACCTGCGCGACCACGAAGGCATCGTGCAGGTGACGGTGGAACCGGTGGCCG
GCGACGACGCCAGCGCAGAGCTGTTCAAGGTGGCCGCCAGCCTGGGCTACGAGGACGTGTTGCAAGTGGAGGGTGTGGTG
CGCGCGCGTCATGCGGTCAACGACAAGCTGCGCAGCGGCAAGGTGGAAGTGATCGCCACGCGCATCAGCATCCTCAACAA
GGCCGCGCCGCTGCCGTTCCACGCGCACGAAAATCCGGGTGAAGAGACGCGCCTGAAATACCGCTACCTGGATCTGCGCC
GGCCGGAGATGCAGCGCATGCAGCGCACCCGCATCAAGCTGGTGCAGGCGCTGCGCCGGCATCTGGATGCGCGCGACTTC
CAGGACATCGAAACCCCGATCCTGACCAAGGCCACCCCGGAAGGCGCGCGCGACTTCCTGGTGCCGGCGCGCATGCATCC
GGGCGAGTTCTACGCGCTGCCGCAGAGCCCGCAGCTGTTCAAGCAGATCCTGATGGTGGCCGGCTTCGACCGCTACTACC
AGATCGCGCGCTGCTTTCGCGATGAAGCGCTACGCGCCGACCGCCAGCTGGAATTCACCCAGCTCGACATGGAATTCGCC
TTCGTGCGCGAGCGCGACGTGCAGGATTTCGTCGAAGACATGATTCGCGCCATCTTCAAGGAAGTGGTGGACGTGGACCT
GGCTGCGCAGTTCCCGCGCATGACCTGGGCCGAGGCGATGCGCCGCTTTGGATCGGACAAGCCGGACCTGCGCATCGCGC
TGGAACTGGTCGACGTGGCCGAGCTGGTCAAGGCCAGCCAATTCCCGGTGTTCACCGCTGCCGCCAACGATGCCGATGGC
CGCGTCGCCGCGCTGCGCATTCCCGGCGGCGCCAGCCTGTCGCGCAAGCAGATCGACGAATACGCAGCGCACGCCGCCAA
ATACGGTGCCAAGGGCCTGGCCTACATCAAGCTGTCGGAGACCGGCGAGGTCAGTTCGCCGATCGCCAAGTTCTTCAGCG
AAGAATCCTTCGCCGCGCTGCTCAAGCATGTAGGCGCCGGCAATGGCGACATCGTGTTCTTCGGTGCCGGCGGCTACACC
AAGGTGTCCGACTTCATGGGCGCGCTGCGCCTGAAGGCCGGCAAGGAATTCGACCTGATCGCCGACGGCTGGGCGCCGCT
GTGGGTCACCGACTTTCCGATGTTCGAATGGGACGAAGAGGCGCAGCGTTACGTCGCCTTGCATCACCCCTTCACCGCAC
CGGCGGTGGACGATATTGCCGACCTGCGCGCCAACGCCCGCACCGCGGTCTCGCGCGGCTACGACATGGTGCTCAATGGC
AATGAAATCGGCGGCGGCTCGATCCGTATCCATCGCCCGGACATGCAGAGCGCCGTGTTCGAACTGCTCGGCATCGGCGC
CGAAGAGGCGCGCGCCAAGTTCGGCTTCCTGCTGGATGCCTTGAACTATGGCGCACCGCCGCACGGCGGCATCGCCTTCG
GTATCGACCGCATCGCCGCGCTGATGGCCGGTACCGAGTCGATTCGCGACGTCATCCCGTTCCCCAAGACCACCGGCGCG
CAGGACCTGATGACCGACGCCCCGTCGCCGATCGCTGCCGACCAGCTCGCCGAAGTGCACGTGCAGGTGCGTCCGAAGCA
GGTCTGA

Upstream 100 bases:

>100_bases
GCGCCCATGAGCGCCCGCGCATTTGCTCCTGTCGCCCGCCGGCCCCAGAATATCCGGCTTTCCGGCGCTGTCCTGCGCCG
GGTTTTCAGCGGAGCTTTCG

Downstream 100 bases:

>100_bases
CCGGCCATCGAACGCACGGCCACGCCCATTGCGTGGCCGTGCGTTCATGCGCAGCGAGCAGCTTGCGCGAGGCTTTGCCG
ACCGATCCGCGCTGCACTCA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MRTHFCGLVDETLIGQTVTLAGWTDVARNLGGVCFIDLRDHEGIVQVTVEPVAGDDASAELFKVAASLGYEDVLQVEGVV
RARHAVNDKLRSGKVEVIATRISILNKAAPLPFHAHENPGEETRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDARDF
QDIETPILTKATPEGARDFLVPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
FVRERDVQDFVEDMIRAIFKEVVDVDLAAQFPRMTWAEAMRRFGSDKPDLRIALELVDVAELVKASQFPVFTAAANDADG
RVAALRIPGGASLSRKQIDEYAAHAAKYGAKGLAYIKLSETGEVSSPIAKFFSEESFAALLKHVGAGNGDIVFFGAGGYT
KVSDFMGALRLKAGKEFDLIADGWAPLWVTDFPMFEWDEEAQRYVALHHPFTAPAVDDIADLRANARTAVSRGYDMVLNG
NEIGGGSIRIHRPDMQSAVFELLGIGAEEARAKFGFLLDALNYGAPPHGGIAFGIDRIAALMAGTESIRDVIPFPKTTGA
QDLMTDAPSPIAADQLAEVHVQVRPKQV

Sequences:

>Translated_588_residues
MRTHFCGLVDETLIGQTVTLAGWTDVARNLGGVCFIDLRDHEGIVQVTVEPVAGDDASAELFKVAASLGYEDVLQVEGVV
RARHAVNDKLRSGKVEVIATRISILNKAAPLPFHAHENPGEETRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDARDF
QDIETPILTKATPEGARDFLVPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
FVRERDVQDFVEDMIRAIFKEVVDVDLAAQFPRMTWAEAMRRFGSDKPDLRIALELVDVAELVKASQFPVFTAAANDADG
RVAALRIPGGASLSRKQIDEYAAHAAKYGAKGLAYIKLSETGEVSSPIAKFFSEESFAALLKHVGAGNGDIVFFGAGGYT
KVSDFMGALRLKAGKEFDLIADGWAPLWVTDFPMFEWDEEAQRYVALHHPFTAPAVDDIADLRANARTAVSRGYDMVLNG
NEIGGGSIRIHRPDMQSAVFELLGIGAEEARAKFGFLLDALNYGAPPHGGIAFGIDRIAALMAGTESIRDVIPFPKTTGA
QDLMTDAPSPIAADQLAEVHVQVRPKQV
>Mature_588_residues
MRTHFCGLVDETLIGQTVTLAGWTDVARNLGGVCFIDLRDHEGIVQVTVEPVAGDDASAELFKVAASLGYEDVLQVEGVV
RARHAVNDKLRSGKVEVIATRISILNKAAPLPFHAHENPGEETRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDARDF
QDIETPILTKATPEGARDFLVPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
FVRERDVQDFVEDMIRAIFKEVVDVDLAAQFPRMTWAEAMRRFGSDKPDLRIALELVDVAELVKASQFPVFTAAANDADG
RVAALRIPGGASLSRKQIDEYAAHAAKYGAKGLAYIKLSETGEVSSPIAKFFSEESFAALLKHVGAGNGDIVFFGAGGYT
KVSDFMGALRLKAGKEFDLIADGWAPLWVTDFPMFEWDEEAQRYVALHHPFTAPAVDDIADLRANARTAVSRGYDMVLNG
NEIGGGSIRIHRPDMQSAVFELLGIGAEEARAKFGFLLDALNYGAPPHGGIAFGIDRIAALMAGTESIRDVIPFPKTTGA
QDLMTDAPSPIAADQLAEVHVQVRPKQV

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=596, Percent_Identity=39.7651006711409, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI45439306, Length=255, Percent_Identity=31.3725490196078, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI5031815, Length=288, Percent_Identity=26.0416666666667, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI194272210, Length=288, Percent_Identity=26.0416666666667, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1788173, Length=593, Percent_Identity=55.1433389544688, Blast_Score=644, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=327, Percent_Identity=27.217125382263, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1790571, Length=286, Percent_Identity=28.3216783216783, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI32566633, Length=610, Percent_Identity=35.2459016393443, Blast_Score=337, Evalue=7e-93,
Organism=Caenorhabditis elegans, GI17551876, Length=266, Percent_Identity=26.6917293233083, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71994340, Length=310, Percent_Identity=24.1935483870968, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=264, Percent_Identity=24.6212121212121, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17535927, Length=264, Percent_Identity=24.6212121212121, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=637, Percent_Identity=32.182103610675, Blast_Score=286, Evalue=9e-78,
Organism=Saccharomyces cerevisiae, GI6323011, Length=250, Percent_Identity=30.8, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320242, Length=257, Percent_Identity=29.1828793774319, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324256, Length=174, Percent_Identity=24.7126436781609, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24584738, Length=603, Percent_Identity=36.9817578772803, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI17136276, Length=265, Percent_Identity=27.9245283018868, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24640849, Length=256, Percent_Identity=27.34375, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24640851, Length=256, Percent_Identity=27.34375, Blast_Score=80, Evalue=5e-15,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_XANAC (Q8PHU5)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643462.1
- ProteinModelPortal:   Q8PHU5
- SMR:   Q8PHU5
- GeneID:   1157225
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3154
- NMPDR:   fig|190486.1.peg.3106
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   XAXO190486:XAC3154-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 65033; Mature: 65033

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTHFCGLVDETLIGQTVTLAGWTDVARNLGGVCFIDLRDHEGIVQVTVEPVAGDDASAE
CCCCHHCHHHHHHCCCEEEECCHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCCCCHHH
LFKVAASLGYEDVLQVEGVVRARHAVNDKLRSGKVEVIATRISILNKAAPLPFHAHENPG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCCCCCC
EETRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDARDFQDIETPILTKATPEGARDFL
HHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEECCCCCCCHHCC
VPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
CCCCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEHHCCHHHH
FVRERDVQDFVEDMIRAIFKEVVDVDLAAQFPRMTWAEAMRRFGSDKPDLRIALELVDVA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEHHHHHHHH
ELVKASQFPVFTAAANDADGRVAALRIPGGASLSRKQIDEYAAHAAKYGAKGLAYIKLSE
HHHHHCCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECC
TGEVSSPIAKFFSEESFAALLKHVGAGNGDIVFFGAGGYTKVSDFMGALRLKAGKEFDLI
CCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEE
ADGWAPLWVTDFPMFEWDEEAQRYVALHHPFTAPAVDDIADLRANARTAVSRGYDMVLNG
ECCCCCCEEECCCCCCCCHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEC
NEIGGGSIRIHRPDMQSAVFELLGIGAEEARAKFGFLLDALNYGAPPHGGIAFGIDRIAA
CEECCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LMAGTESIRDVIPFPKTTGAQDLMTDAPSPIAADQLAEVHVQVRPKQV
HHHCHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHEEEEECCCCC
>Mature Secondary Structure
MRTHFCGLVDETLIGQTVTLAGWTDVARNLGGVCFIDLRDHEGIVQVTVEPVAGDDASAE
CCCCHHCHHHHHHCCCEEEECCHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCCCCHHH
LFKVAASLGYEDVLQVEGVVRARHAVNDKLRSGKVEVIATRISILNKAAPLPFHAHENPG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCCCCCC
EETRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDARDFQDIETPILTKATPEGARDFL
HHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEECCCCCCCHHCC
VPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
CCCCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEHHCCHHHH
FVRERDVQDFVEDMIRAIFKEVVDVDLAAQFPRMTWAEAMRRFGSDKPDLRIALELVDVA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEHHHHHHHH
ELVKASQFPVFTAAANDADGRVAALRIPGGASLSRKQIDEYAAHAAKYGAKGLAYIKLSE
HHHHHCCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECC
TGEVSSPIAKFFSEESFAALLKHVGAGNGDIVFFGAGGYTKVSDFMGALRLKAGKEFDLI
CCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEE
ADGWAPLWVTDFPMFEWDEEAQRYVALHHPFTAPAVDDIADLRANARTAVSRGYDMVLNG
ECCCCCCEEECCCCCCCCHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEC
NEIGGGSIRIHRPDMQSAVFELLGIGAEEARAKFGFLLDALNYGAPPHGGIAFGIDRIAA
CEECCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LMAGTESIRDVIPFPKTTGAQDLMTDAPSPIAADQLAEVHVQVRPKQV
HHHCHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217