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The map label for this gene is celF [H]

Identifier: 21243808

GI number: 21243808

Start: 3614460

End: 3615884

Strand: Direct

Name: celF [H]

Synonym: XAC3081

Alternate gene names: 21243808

Gene position: 3614460-3615884 (Clockwise)

Preceding gene: 21243807

Following gene: 21243809

Centisome position: 69.84

GC content: 70.18

Gene sequence:

>1425_bases
ATGAGCAGTTCTGTCGACAATCGCAAGCTCACCCTGATCGGCGGCGGCGGCGTCCGCACACCCTTGGTGGTGTACGGCGT
CAACGAAGCCGCCGAGGCGCTGGGCGCGCGCGAGATCGTGCTGTACGACCCCGATCAGGAGCGGCTGGCGCTGATGGTGG
CGATGGGCCGCGCCGTCGTGGCCGACGCCGGCGGCGCGTTGCAGGTGCATGCGGCGCCCTCGCTGGAAGCGGCGATCGAA
GGCGCGCATTTCGTGCTCAACAGCATCCGCGTCGGCGGCATCGCCCAGCGCGCCTCCGATGAGCAGACCATCATCGGCCA
TGGCTATTCCGGCCAGGAGACCACCGGCCCGGGCGGCGTGGCCAAGGCGCTGCGCACGGTACCGGTGGCGATCGAACAGG
CGCGCATGATCGAACGCCTGAGCCCGGACGCCTGGCTGGTGAGCTTCACCAACCCCGCCGGGCTGATCACCCAGGCCATC
AGCGCACACACCCGCGCGCGCGTGGTCGGTATCTGCGATACGCCGGTGGAGTTGTTCCACAACATCGCACGCGCGCTCGG
CGAGCCGGCTGCCGATGTGGAATGCGACTACGTCGGGCTCAATCATCTGGGCTGGATCCGCGCGGTGCGCCTGCGCGGCG
AAGACATCCTCGACCGCGTGCTCGCCGACGACGCCATCCTGCGCCAGCTCTACCTGGCGCCCTTGTTCGACTTCGACATG
CTGCGCGCACTGCGGCTGATCCCCACCGAATACCTGTATTTCTTCTACGAGCGCCGCCGCGCCCTGGAGAACCAGCGCGC
CAGCGGCGCGAGCCGTGGCGGCGAGATCGAGCGGCTCAACCGCACCCTCATCGGCGACCTGCGCAGCCGGCTGGACGCCG
GCGACACCACCGGTGCGGTACAGACCTATGCGGCCTATCTGGCGCAGCGCTCCGGCTCGTACATGAAGCTGGAGGCCGAG
GCCGGCTCGGCCTTCGCGCCGGACCTGGCGCCGCAGCCGGACCCGTTCCGCGCCAGCACCGGCTATCACCGCATCGCGGT
GGACGTGATGAGCGCGCTGACCGGCGCCCGGCCCGCGCGGCATGTCGTCAATGTGCGCAACCAGGGCGCGATGGGCGAGC
TGGCCGACGACGACATCGTGGAAATCGCCGCAGACATCGATCGCGACCGCATCGTGCCGGTCCCGGCCGCGCCGCTGCCC
AGCGCCGTGCAGGGGCTGGTGCACACGTTCAAGGCGTACGAGCACGCCGCCATCGAGGCGGCGCTCAGCGGCAGCCATCT
GGACGCCTGCAAGGCGATGCTGATCCACCCGGCGATCGGCGAATGGAGCCCGGCGCGGCGGTTGCTGGACGAGTTGTTCG
GCCACGCACACGACGATGGCCACTGCGCGGGGCCGGCACAGTGGCGTGGACATGCGGTGGGCTGA

Upstream 100 bases:

>100_bases
GCGCACAGCACGGCTGCCACGCGGGCGCGGCCTGCTGCAGCGCGCTCGGTGCGCTCGACGGCCTGTTTCTCCTATCTCCC
CCCACACACGAGGACGTTCC

Downstream 100 bases:

>100_bases
TACCGAGGTTGATGCCGTGCCGTCGCTGCCGGTGGGGTCATGACACTGGCGTGCCGGTCGTCGGCGCGTTTCAACGCCGC
CGGCAACGAAGACCCAGCGC

Product: 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSSSVDNRKLTLIGGGGVRTPLVVYGVNEAAEALGAREIVLYDPDQERLALMVAMGRAVVADAGGALQVHAAPSLEAAIE
GAHFVLNSIRVGGIAQRASDEQTIIGHGYSGQETTGPGGVAKALRTVPVAIEQARMIERLSPDAWLVSFTNPAGLITQAI
SAHTRARVVGICDTPVELFHNIARALGEPAADVECDYVGLNHLGWIRAVRLRGEDILDRVLADDAILRQLYLAPLFDFDM
LRALRLIPTEYLYFFYERRRALENQRASGASRGGEIERLNRTLIGDLRSRLDAGDTTGAVQTYAAYLAQRSGSYMKLEAE
AGSAFAPDLAPQPDPFRASTGYHRIAVDVMSALTGARPARHVVNVRNQGAMGELADDDIVEIAADIDRDRIVPVPAAPLP
SAVQGLVHTFKAYEHAAIEAALSGSHLDACKAMLIHPAIGEWSPARRLLDELFGHAHDDGHCAGPAQWRGHAVG

Sequences:

>Translated_474_residues
MSSSVDNRKLTLIGGGGVRTPLVVYGVNEAAEALGAREIVLYDPDQERLALMVAMGRAVVADAGGALQVHAAPSLEAAIE
GAHFVLNSIRVGGIAQRASDEQTIIGHGYSGQETTGPGGVAKALRTVPVAIEQARMIERLSPDAWLVSFTNPAGLITQAI
SAHTRARVVGICDTPVELFHNIARALGEPAADVECDYVGLNHLGWIRAVRLRGEDILDRVLADDAILRQLYLAPLFDFDM
LRALRLIPTEYLYFFYERRRALENQRASGASRGGEIERLNRTLIGDLRSRLDAGDTTGAVQTYAAYLAQRSGSYMKLEAE
AGSAFAPDLAPQPDPFRASTGYHRIAVDVMSALTGARPARHVVNVRNQGAMGELADDDIVEIAADIDRDRIVPVPAAPLP
SAVQGLVHTFKAYEHAAIEAALSGSHLDACKAMLIHPAIGEWSPARRLLDELFGHAHDDGHCAGPAQWRGHAVG
>Mature_473_residues
SSSVDNRKLTLIGGGGVRTPLVVYGVNEAAEALGAREIVLYDPDQERLALMVAMGRAVVADAGGALQVHAAPSLEAAIEG
AHFVLNSIRVGGIAQRASDEQTIIGHGYSGQETTGPGGVAKALRTVPVAIEQARMIERLSPDAWLVSFTNPAGLITQAIS
AHTRARVVGICDTPVELFHNIARALGEPAADVECDYVGLNHLGWIRAVRLRGEDILDRVLADDAILRQLYLAPLFDFDML
RALRLIPTEYLYFFYERRRALENQRASGASRGGEIERLNRTLIGDLRSRLDAGDTTGAVQTYAAYLAQRSGSYMKLEAEA
GSAFAPDLAPQPDPFRASTGYHRIAVDVMSALTGARPARHVVNVRNQGAMGELADDDIVEIAADIDRDRIVPVPAAPLPS
AVQGLVHTFKAYEHAAIEAALSGSHLDACKAMLIHPAIGEWSPARRLLDELFGHAHDDGHCAGPAQWRGHAVG

Specific function: Hydrolyzes phospho-beta-glucosides (Probable) [H]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=446, Percent_Identity=27.8026905829596, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1790560, Length=441, Percent_Identity=24.4897959183673, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 50808; Mature: 50677

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSVDNRKLTLIGGGGVRTPLVVYGVNEAAEALGAREIVLYDPDQERLALMVAMGRAVV
CCCCCCCCEEEEEECCCCCCCEEEEECCHHHHHCCCCEEEEECCCHHHHHHHHHHCCHHE
ADAGGALQVHAAPSLEAAIEGAHFVLNSIRVGGIAQRASDEQTIIGHGYSGQETTGPGGV
ECCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEECCCCCCCCCCCHHH
AKALRTVPVAIEQARMIERLSPDAWLVSFTNPAGLITQAISAHTRARVVGICDTPVELFH
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHH
NIARALGEPAADVECDYVGLNHLGWIRAVRLRGEDILDRVLADDAILRQLYLAPLFDFDM
HHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHH
LRALRLIPTEYLYFFYERRRALENQRASGASRGGEIERLNRTLIGDLRSRLDAGDTTGAV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHH
QTYAAYLAQRSGSYMKLEAEAGSAFAPDLAPQPDPFRASTGYHRIAVDVMSALTGARPAR
HHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHH
HVVNVRNQGAMGELADDDIVEIAADIDRDRIVPVPAAPLPSAVQGLVHTFKAYEHAAIEA
HHHHCCCCCCCCCCCCCHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
ALSGSHLDACKAMLIHPAIGEWSPARRLLDELFGHAHDDGHCAGPAQWRGHAVG
HHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSSVDNRKLTLIGGGGVRTPLVVYGVNEAAEALGAREIVLYDPDQERLALMVAMGRAVV
CCCCCCCEEEEEECCCCCCCEEEEECCHHHHHCCCCEEEEECCCHHHHHHHHHHCCHHE
ADAGGALQVHAAPSLEAAIEGAHFVLNSIRVGGIAQRASDEQTIIGHGYSGQETTGPGGV
ECCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEECCCCCCCCCCCHHH
AKALRTVPVAIEQARMIERLSPDAWLVSFTNPAGLITQAISAHTRARVVGICDTPVELFH
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHH
NIARALGEPAADVECDYVGLNHLGWIRAVRLRGEDILDRVLADDAILRQLYLAPLFDFDM
HHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHH
LRALRLIPTEYLYFFYERRRALENQRASGASRGGEIERLNRTLIGDLRSRLDAGDTTGAV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHH
QTYAAYLAQRSGSYMKLEAEAGSAFAPDLAPQPDPFRASTGYHRIAVDVMSALTGARPAR
HHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHH
HVVNVRNQGAMGELADDDIVEIAADIDRDRIVPVPAAPLPSAVQGLVHTFKAYEHAAIEA
HHHHCCCCCCCCCCCCCHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
ALSGSHLDACKAMLIHPAIGEWSPARRLLDELFGHAHDDGHCAGPAQWRGHAVG
HHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990303; 9384377; 8969509 [H]