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The map label for this gene is kpsF [H]

Identifier: 21243694

GI number: 21243694

Start: 3470768

End: 3471769

Strand: Direct

Name: kpsF [H]

Synonym: XAC2967

Alternate gene names: 21243694

Gene position: 3470768-3471769 (Clockwise)

Preceding gene: 77748685

Following gene: 21243695

Centisome position: 67.06

GC content: 70.86

Gene sequence:

>1002_bases
ATGGCTGTCTCGCACCTGCCCTCCGCCACCGTCAGCGACGCCACCCTGGTCGCCAGCGGCCAGCGCGTGCTGGAGATCGA
GCGCGAGGCGCTGGCCAGCGTGGGCGCACGCATCGGCCGCGAGTTCGCCGCGGCCTGCCGGCTGGTGCTGACCTCGCGCG
GCCGGGTGGTCGCCACCGGCATGGGCAAGTCCGGCCATGTGGCGCGCAAGATCGCCGCCACCCTCGCCTCGACCGGCACC
CCGGCGTTCTTCGTGCACCCGGGCGAAGCCGGACACGGCGACCTGGGCATGATCACCGAGGCCGACATCGTGCTGGCGCT
GTCGTACTCCGGGGAATCGGACGAAGTGCGCATGCTGCTGCCGGTGCTCAAGCGCCAGGGCAACCCGATCATCGCCATGA
CCGGCCGCACCAGTTCCACCCTGGCCCAGGCCGCCGACGTGCACCTGGACGTCAGCGTGTCGGCCGAAGCCTGCCCGCTG
CATCTGGCCCCCACCTCCAGCACCACCGCCTCGCTGGCGATGGGCGATGCACTGGCGGTTGCGCTGCTGGATGCGCGCGG
CTTCACCGCCGACGACTTCGCGCGCTCGCACCCGGCCGGCAGCCTGGGCCGGCGCCTGCTGCTGCACATCACCGATGTGA
TGCACGCCGGCGAGGACCTGCCGCGTGTGCGCGAAGACGCCAGCCTGAGCGAGGCGTTGATGGAAATGAGCCGCAAGCGG
CTGGGCATGACTGCCGTGGTGGACGCCGACGGCCATCTGATCGGGCTGTTCACCGACGGCGACCTGCGCCGCGCGCTGGA
CAGCGACATCGACGTGCGCAGCGCCGGCATCGCGCAGGTGATGACGCGCAACCCACGCACCATCGGCGCCGACCAGCTGG
CCGCCGAGGCCGCGCGGCTCATGGAGGACTACAAGATCAACGGCCTGATCGTGGTGGATGCGCAGCACCGCGCGGTGGGC
GCATTGAACATTCACGACCTGTTGCGCGCCAAGGTGGTTTAA

Upstream 100 bases:

>100_bases
TCGGGCAAGCCGGATTCGATGAGTTTACGGATGGTTTCGGCGTCCAAAGGGCGTTTCCTCATAAAATGAGCGCACATTCT
ACTCTTCGCCCGAGTCCTGC

Downstream 100 bases:

>100_bases
CAGGTTCAGTTTTCAACCGCCGGACCCCGTTCTCACCGATTCGTGCCGATGCCCTATTCCCCTTTGCAGCACCTTCCAGC
CGAGCTGATCGAGCGCGCCG

Product: polysialic acid capsule expression protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MAVSHLPSATVSDATLVASGQRVLEIEREALASVGARIGREFAAACRLVLTSRGRVVATGMGKSGHVARKIAATLASTGT
PAFFVHPGEAGHGDLGMITEADIVLALSYSGESDEVRMLLPVLKRQGNPIIAMTGRTSSTLAQAADVHLDVSVSAEACPL
HLAPTSSTTASLAMGDALAVALLDARGFTADDFARSHPAGSLGRRLLLHITDVMHAGEDLPRVREDASLSEALMEMSRKR
LGMTAVVDADGHLIGLFTDGDLRRALDSDIDVRSAGIAQVMTRNPRTIGADQLAAEAARLMEDYKINGLIVVDAQHRAVG
ALNIHDLLRAKVV

Sequences:

>Translated_333_residues
MAVSHLPSATVSDATLVASGQRVLEIEREALASVGARIGREFAAACRLVLTSRGRVVATGMGKSGHVARKIAATLASTGT
PAFFVHPGEAGHGDLGMITEADIVLALSYSGESDEVRMLLPVLKRQGNPIIAMTGRTSSTLAQAADVHLDVSVSAEACPL
HLAPTSSTTASLAMGDALAVALLDARGFTADDFARSHPAGSLGRRLLLHITDVMHAGEDLPRVREDASLSEALMEMSRKR
LGMTAVVDADGHLIGLFTDGDLRRALDSDIDVRSAGIAQVMTRNPRTIGADQLAAEAARLMEDYKINGLIVVDAQHRAVG
ALNIHDLLRAKVV
>Mature_332_residues
AVSHLPSATVSDATLVASGQRVLEIEREALASVGARIGREFAAACRLVLTSRGRVVATGMGKSGHVARKIAATLASTGTP
AFFVHPGEAGHGDLGMITEADIVLALSYSGESDEVRMLLPVLKRQGNPIIAMTGRTSSTLAQAADVHLDVSVSAEACPLH
LAPTSSTTASLAMGDALAVALLDARGFTADDFARSHPAGSLGRRLLLHITDVMHAGEDLPRVREDASLSEALMEMSRKRL
GMTAVVDADGHLIGLFTDGDLRRALDSDIDVRSAGIAQVMTRNPRTIGADQLAAEAARLMEDYKINGLIVVDAQHRAVGA
LNIHDLLRAKVV

Specific function: Catalyzes the interconversion of D-arabinose 5-phosphate and D-ribulose 5-phosphate [H]

COG id: COG0794

COG function: function code M; Predicted sugar phosphate isomerase involved in capsule formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SIS domain [H]

Homologues:

Organism=Escherichia coli, GI1789588, Length=315, Percent_Identity=57.1428571428571, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI87082151, Length=318, Percent_Identity=45.2830188679245, Blast_Score=275, Evalue=4e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000644
- InterPro:   IPR004800
- InterPro:   IPR001347 [H]

Pfam domain/function: PF00571 CBS; PF01380 SIS [H]

EC number: =5.3.1.13 [H]

Molecular weight: Translated: 34914; Mature: 34783

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSHLPSATVSDATLVASGQRVLEIEREALASVGARIGREFAAACRLVLTSRGRVVATG
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
MGKSGHVARKIAATLASTGTPAFFVHPGEAGHGDLGMITEADIVLALSYSGESDEVRMLL
CCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEECEEEEEEEECCCCCHHHHHH
PVLKRQGNPIIAMTGRTSSTLAQAADVHLDVSVSAEACPLHLAPTSSTTASLAMGDALAV
HHHHCCCCCEEEECCCCHHHHHHHHCEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHEE
ALLDARGFTADDFARSHPAGSLGRRLLLHITDVMHAGEDLPRVREDASLSEALMEMSRKR
EEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHH
LGMTAVVDADGHLIGLFTDGDLRRALDSDIDVRSAGIAQVMTRNPRTIGADQLAAEAARL
CCCEEEEECCCCEEEEEECCHHHHHHCCCCCCHHCCHHHHHHCCCCCCCHHHHHHHHHHH
MEDYKINGLIVVDAQHRAVGALNIHDLLRAKVV
HHHCCCCEEEEEECCCCEEEECCHHHHHHHHCC
>Mature Secondary Structure 
AVSHLPSATVSDATLVASGQRVLEIEREALASVGARIGREFAAACRLVLTSRGRVVATG
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
MGKSGHVARKIAATLASTGTPAFFVHPGEAGHGDLGMITEADIVLALSYSGESDEVRMLL
CCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEECEEEEEEEECCCCCHHHHHH
PVLKRQGNPIIAMTGRTSSTLAQAADVHLDVSVSAEACPLHLAPTSSTTASLAMGDALAV
HHHHCCCCCEEEECCCCHHHHHHHHCEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHEE
ALLDARGFTADDFARSHPAGSLGRRLLLHITDVMHAGEDLPRVREDASLSEALMEMSRKR
EEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHH
LGMTAVVDADGHLIGLFTDGDLRRALDSDIDVRSAGIAQVMTRNPRTIGADQLAAEAARL
CCCEEEEECCCCEEEEEECCHHHHHHCCCCCCHHCCHHHHHHCCCCCCCHHHHHHHHHHH
MEDYKINGLIVVDAQHRAVGALNIHDLLRAKVV
HHHCCCCEEEEEECCCCEEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]