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The map label for this gene is purN [H]

Identifier: 21243688

GI number: 21243688

Start: 3466187

End: 3466855

Strand: Direct

Name: purN [H]

Synonym: XAC2961

Alternate gene names: 21243688

Gene position: 3466187-3466855 (Clockwise)

Preceding gene: 21243687

Following gene: 77748684

Centisome position: 66.97

GC content: 69.51

Gene sequence:

>669_bases
ATGCCCGCACCTGACGCCCCATTACGCCTGGCCGTACTGGCCTCCGGCCGTGGCAGCAATCTGCAGGCCATTGTCGATGC
GATCGCCAGTGGGCGTCTGCATGCCGAGGTGGTCGGCGTGTTTTCCGATCGCCCGCAGGCGCCAGCCTTGCAGAAGGTCG
AGCCGGCGCGTCGCTGGAGCGCCAGCCCGCGCGATTTCGCCGACCGTGCCGCGTTCGATGCCGCACTTGGCCAGGCGATC
GCGGCAGCGCAGCCGGACTGGGTGATCTGCGCCGGCTACATGCGCATCCTGGGCGAGCCGTTGGTGCGCCGTTTCGCCGG
GCGCATGCTCAACATCCATCCCTCGCTGCTACCCAAATACCGCGGCCTGCACACGCACGCGCGCGCGCTGGAAGCCGGCG
ATGCAGAGCATGGTGCCAGCGTGCATCTGGTGGTGCCGGAGCTGGATGCCGGCGCGGTGATCGCGCAGGCGCGCGTCCCG
GTCCTGCCCGGCGACAGTGCCGAACAACTGGCGGCGCGGGTGCTGGCGCGCGAGCACCCGCTGCTCCTGGCCACGCTGGA
GGTGCTTGCCAGTGGCCGCGTCGCGGTGCATGGCGACACCGTGCTTATCGACGGTCAGTGCCTGTTTACGCCACTGCGAC
TAGAGTCCGCAAACACCGCACTCGCCTGA

Upstream 100 bases:

>100_bases
TTGGCGTCTTTACGCTGGTGACGCTGCTGCGCCCGCAGGTCAAGGCTCTGTTCGGTGTCGGCAACGCTGCGGCTTGAGAT
GTTTGTGAGCCTACTCTCCA

Downstream 100 bases:

>100_bases
ATGCGGAAGACATCTCCCTCATCCCGTCTCGGACATCCTTGCGGCCTCCGTGCCCGCCACCAGCGCATGAACGTCATCCC
GATCGCCGCCGCGCTGTTAA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MPAPDAPLRLAVLASGRGSNLQAIVDAIASGRLHAEVVGVFSDRPQAPALQKVEPARRWSASPRDFADRAAFDAALGQAI
AAAQPDWVICAGYMRILGEPLVRRFAGRMLNIHPSLLPKYRGLHTHARALEAGDAEHGASVHLVVPELDAGAVIAQARVP
VLPGDSAEQLAARVLAREHPLLLATLEVLASGRVAVHGDTVLIDGQCLFTPLRLESANTALA

Sequences:

>Translated_222_residues
MPAPDAPLRLAVLASGRGSNLQAIVDAIASGRLHAEVVGVFSDRPQAPALQKVEPARRWSASPRDFADRAAFDAALGQAI
AAAQPDWVICAGYMRILGEPLVRRFAGRMLNIHPSLLPKYRGLHTHARALEAGDAEHGASVHLVVPELDAGAVIAQARVP
VLPGDSAEQLAARVLAREHPLLLATLEVLASGRVAVHGDTVLIDGQCLFTPLRLESANTALA
>Mature_221_residues
PAPDAPLRLAVLASGRGSNLQAIVDAIASGRLHAEVVGVFSDRPQAPALQKVEPARRWSASPRDFADRAAFDAALGQAIA
AAQPDWVICAGYMRILGEPLVRRFAGRMLNIHPSLLPKYRGLHTHARALEAGDAEHGASVHLVVPELDAGAVIAQARVPV
LPGDSAEQLAARVLAREHPLLLATLEVLASGRVAVHGDTVLIDGQCLFTPLRLESANTALA

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=190, Percent_Identity=40.5263157894737, Blast_Score=138, Evalue=5e-33,
Organism=Homo sapiens, GI209869995, Length=190, Percent_Identity=40.5263157894737, Blast_Score=138, Evalue=5e-33,
Organism=Homo sapiens, GI209869993, Length=190, Percent_Identity=40.5263157894737, Blast_Score=138, Evalue=5e-33,
Organism=Escherichia coli, GI1788846, Length=203, Percent_Identity=46.3054187192118, Blast_Score=177, Evalue=4e-46,
Organism=Escherichia coli, GI1787483, Length=135, Percent_Identity=34.8148148148148, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788589, Length=117, Percent_Identity=31.6239316239316, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17567511, Length=175, Percent_Identity=32, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320616, Length=208, Percent_Identity=32.6923076923077, Blast_Score=82, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24582400, Length=188, Percent_Identity=45.7446808510638, Blast_Score=154, Evalue=6e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23399; Mature: 23268

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPDAPLRLAVLASGRGSNLQAIVDAIASGRLHAEVVGVFSDRPQAPALQKVEPARRWS
CCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHCCHHHHCC
ASPRDFADRAAFDAALGQAIAAAQPDWVICAGYMRILGEPLVRRFAGRMLNIHPSLLPKY
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCEEECCHHHHHHH
RGLHTHARALEAGDAEHGASVHLVVPELDAGAVIAQARVPVLPGDSAEQLAARVLAREHP
CCHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCC
LLLATLEVLASGRVAVHGDTVLIDGQCLFTPLRLESANTALA
HHHHHHHHHHCCCEEEECCEEEECCCEEEEEEEECCCCCCCC
>Mature Secondary Structure 
PAPDAPLRLAVLASGRGSNLQAIVDAIASGRLHAEVVGVFSDRPQAPALQKVEPARRWS
CCCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHCCHHHHCC
ASPRDFADRAAFDAALGQAIAAAQPDWVICAGYMRILGEPLVRRFAGRMLNIHPSLLPKY
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCEEECCHHHHHHH
RGLHTHARALEAGDAEHGASVHLVVPELDAGAVIAQARVPVLPGDSAEQLAARVLAREHP
CCHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCC
LLLATLEVLASGRVAVHGDTVLIDGQCLFTPLRLESANTALA
HHHHHHHHHHCCCEEEECCEEEECCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]