Click here to switch to the map view.

The map label for this gene is pilU [H]

Identifier: 21243650

GI number: 21243650

Start: 3429688

End: 3430818

Strand: Reverse

Name: pilU [H]

Synonym: XAC2923

Alternate gene names: 21243650

Gene position: 3430818-3429688 (Counterclockwise)

Preceding gene: 21243651

Following gene: 21243648

Centisome position: 66.29

GC content: 62.16

Gene sequence:

>1131_bases
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGCCCGATTACCCAGACCCCGCTGACCGCGCAGCAGAGTCGCGATCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGTTTTCGTGTCAGCTGCTTCTACCAGCGTAATCAGGTGGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCCTGCCGCCGGTGATCAAGACGCTGTCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGGACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGGCACATCATCACC
ATCGAGGACCCGATCGAATTCGTGCACAAGCACGAGGGTTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGTTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCATGCGATCGCATTCGCCGAAACCGGCCATCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAAGATCGCCGCAACCAGCTGTTGATGGACCTGTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGATTCCGACCCCGGACGGGCGCAGCCGTCGCGTGGCGATGGAAATCATGTTGGGCACGCCGCTGGTGC
AGGACTACATACGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAAATCAGTTACGAAGACGCGTTGCGCTACGCCGACTCGCAGAACGA
GGTCCGCCTGCGCATCAAGCTCTCGCAGGGCGGCGATGCCAAGACCTTGTCGCAGGGCCTGGACGGCGTGGAAATTTCCG
AAGTGCGCTGA

Upstream 100 bases:

>100_bases
TGGGGAATCGCAAAGGCGCGTTCCCGTCACCGAGTCCGTTGCCCGGCTTCTCCAATTCCCGTTTCTCGATTCCCCATTCC
CGGCTCCGAGGGAGCACCCC

Downstream 100 bases:

>100_bases
GTCGCGCGCGGTCGCGCCGGTGACTCAGTTGCCCGCTCCCCTCCAAGGGTGAGCGGGCGCCCCGTCCTCATCGTGCCGTG
CCAGTGTTTTCAACCTGTAT

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLSMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEISEVR

Sequences:

>Translated_376_residues
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLSMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEISEVR
>Mature_375_residues
STIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSGV
GRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLSMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIITI
EDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAMD
RIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTFD
QSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEISEVR

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=321, Percent_Identity=34.2679127725857, Blast_Score=217, Evalue=8e-58,
Organism=Escherichia coli, GI1789723, Length=146, Percent_Identity=40.4109589041096, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1786296, Length=261, Percent_Identity=26.4367816091954, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 42045; Mature: 41914

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTP
CCCCCHHHHHHHHHHCCCCCEEEECCCCCCEEEECEECCCCCCCCCCCCCCCEEEEEECC
SQREEFEKTHECNFAIGVSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKT
CHHHHHHHHHCCCEEEECCCCCEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHH
LSMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIITIEDPIEFVHKHEGCIITQRE
HHHCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCEEEECCHHHHHHCCCCEEEEECC
VGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
CCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEECCCCEEEEEEECCCHHHHH
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDG
HHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHCCC
EIHKLKEIMKESTNLGMRTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDA
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEEECCCCCH
KTLSQGLDGVEISEVR
HHHHHCCCCCEECCCC
>Mature Secondary Structure 
STIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTP
CCCCHHHHHHHHHHCCCCCEEEECCCCCCEEEECEECCCCCCCCCCCCCCCEEEEEECC
SQREEFEKTHECNFAIGVSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKT
CHHHHHHHHHCCCEEEECCCCCEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHH
LSMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIITIEDPIEFVHKHEGCIITQRE
HHHCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCEEEECCHHHHHHCCCCEEEEECC
VGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
CCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEECCCCEEEEEEECCCHHHHH
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDG
HHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHCCC
EIHKLKEIMKESTNLGMRTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDA
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEEECCCCCH
KTLSQGLDGVEISEVR
HHHHHCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]