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The map label for this gene is nfi

Identifier: 21243602

GI number: 21243602

Start: 3374560

End: 3375273

Strand: Direct

Name: nfi

Synonym: XAC2875

Alternate gene names: 21243602

Gene position: 3374560-3375273 (Clockwise)

Preceding gene: 161485709

Following gene: 77748677

Centisome position: 65.2

GC content: 69.19

Gene sequence:

>714_bases
ATGCAGACCAACGACCCCGTCTTCGCCGGTTGGGACGGCAGCGTGATCCAGGCGCGACAGTTGCAACGGCAACTGGCACG
CCGCGTGGTACTCGATGACGCGGTGAGCGCCACGCCGCAGTTGCTTGCAGGGTTCGATGTCGGTTTCGAAGACGATGGCC
AGACCACCCGCGCGGCCGCCGTGTTGCTGGATGCGCAGACGTTGCTGCCGCTGGAAACGCACATCGCGCGCGTGCCGACC
TCGATGCCGTATGTCCCTGGCCTGCTCAGTTTTCGCGAATTGCCGGCATTGCTGCAGGCCCTGGCGCTGCTGTCGCGCAC
GCCGGACCTGGTGTTCATCGATGGCCAGGGCATCGCGCATCCACGCAAGCTTGGCATCGCGGCGCACTTCGGCGTGGTGA
CCGGCCTGCCGTGCATCGGCATCGCCAAGCAGCGCCTGGCCGGCAGCTTCGCCGAGCCTGGGCCCGAGCGCGGCGACCAC
ACGCCGATCCTGCTGGGCGGCTCGCAGATCGGCTGGGCGCTGCGCAGCAAACCGCGCTGCAATCCCTTGATCGTCTCGCC
CGGCCATCGCGTGTCGATGCAAGGCGCACTCGACTGGACCCTGCGCACGTTGCGCGCCTATCGCCTGCCCGAGCCCACGC
GCCTGGCCGACCGGCTGGCCTCGCGCCGCGGCGAGGTGACGGTGCCGGCAGGCTATTCCGGCCACCTGCTGTGA

Upstream 100 bases:

>100_bases
CGGTTCTCCGATTGGGGCCGATCAGCGTGCGATTGTAGCGGCAGCGCGTGCATCGCAGGCGTGCACGAGATCGCCACATG
CAGCGCGCGATGCTTTGGCA

Downstream 100 bases:

>100_bases
GCAGACGTTCAAGCCGCGTCAGGCGCCGCATGCACGGGCGGACGCACTGACACGGCGCTGAGGCGATCAATCAGTTTTCA
GTGATGGCGATGCGACCGGC

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MQTNDPVFAGWDGSVIQARQLQRQLARRVVLDDAVSATPQLLAGFDVGFEDDGQTTRAAAVLLDAQTLLPLETHIARVPT
SMPYVPGLLSFRELPALLQALALLSRTPDLVFIDGQGIAHPRKLGIAAHFGVVTGLPCIGIAKQRLAGSFAEPGPERGDH
TPILLGGSQIGWALRSKPRCNPLIVSPGHRVSMQGALDWTLRTLRAYRLPEPTRLADRLASRRGEVTVPAGYSGHLL

Sequences:

>Translated_237_residues
MQTNDPVFAGWDGSVIQARQLQRQLARRVVLDDAVSATPQLLAGFDVGFEDDGQTTRAAAVLLDAQTLLPLETHIARVPT
SMPYVPGLLSFRELPALLQALALLSRTPDLVFIDGQGIAHPRKLGIAAHFGVVTGLPCIGIAKQRLAGSFAEPGPERGDH
TPILLGGSQIGWALRSKPRCNPLIVSPGHRVSMQGALDWTLRTLRAYRLPEPTRLADRLASRRGEVTVPAGYSGHLL
>Mature_237_residues
MQTNDPVFAGWDGSVIQARQLQRQLARRVVLDDAVSATPQLLAGFDVGFEDDGQTTRAAAVLLDAQTLLPLETHIARVPT
SMPYVPGLLSFRELPALLQALALLSRTPDLVFIDGQGIAHPRKLGIAAHFGVVTGLPCIGIAKQRLAGSFAEPGPERGDH
TPILLGGSQIGWALRSKPRCNPLIVSPGHRVSMQGALDWTLRTLRAYRLPEPTRLADRLASRRGEVTVPAGYSGHLL

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family

Homologues:

Organism=Homo sapiens, GI31542775, Length=197, Percent_Identity=37.5634517766497, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI257467548, Length=121, Percent_Identity=39.6694214876033, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI257467550, Length=121, Percent_Identity=39.6694214876033, Blast_Score=78, Evalue=6e-15,
Organism=Escherichia coli, GI87082357, Length=206, Percent_Identity=52.4271844660194, Blast_Score=221, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFI_XANAC (Q8PIM0)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643184.1
- ProteinModelPortal:   Q8PIM0
- GeneID:   1156946
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC2875
- NMPDR:   fig|190486.1.peg.2828
- HOGENOM:   HBG716360
- OMA:   MPYIPGL
- ProtClustDB:   PRK11617
- BioCyc:   XAXO190486:XAC2875-MONOMER
- BRENDA:   3.1.21.7
- GO:   GO:0005737
- HAMAP:   MF_00801
- InterPro:   IPR007581

Pfam domain/function: PF04493 Endonuclease_5

EC number: =3.1.21.7

Molecular weight: Translated: 25509; Mature: 25509

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTNDPVFAGWDGSVIQARQLQRQLARRVVLDDAVSATPQLLAGFDVGFEDDGQTTRAAA
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHH
VLLDAQTLLPLETHIARVPTSMPYVPGLLSFRELPALLQALALLSRTPDLVFIDGQGIAH
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
PRKLGIAAHFGVVTGLPCIGIAKQRLAGSFAEPGPERGDHTPILLGGSQIGWALRSKPRC
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCHHHCCCCCC
NPLIVSPGHRVSMQGALDWTLRTLRAYRLPEPTRLADRLASRRGEVTVPAGYSGHLL
CEEEECCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEECCCCCCCCC
>Mature Secondary Structure
MQTNDPVFAGWDGSVIQARQLQRQLARRVVLDDAVSATPQLLAGFDVGFEDDGQTTRAAA
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHH
VLLDAQTLLPLETHIARVPTSMPYVPGLLSFRELPALLQALALLSRTPDLVFIDGQGIAH
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
PRKLGIAAHFGVVTGLPCIGIAKQRLAGSFAEPGPERGDHTPILLGGSQIGWALRSKPRC
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCHHHCCCCCC
NPLIVSPGHRVSMQGALDWTLRTLRAYRLPEPTRLADRLASRRGEVTVPAGYSGHLL
CEEEECCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217