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The map label for this gene is vieA [H]

Identifier: 21243595

GI number: 21243595

Start: 3365621

End: 3366904

Strand: Direct

Name: vieA [H]

Synonym: XAC2868

Alternate gene names: 21243595

Gene position: 3365621-3366904 (Clockwise)

Preceding gene: 21243594

Following gene: 21243596

Centisome position: 65.03

GC content: 67.68

Gene sequence:

>1284_bases
ATGCGCCCCGCCCTTCTCTCCTGCAACTGCACGCTGGCCGGCCCGGTCCTGGTCGTGGACGACAGCGTGGTCCAGCGCGA
ACACGCCATGGCGCTATGTCGCCAGCTCGGCGCAAGCGTGGTCGACGGTGCCGTCGACGGAAACGCGGCCCTGGAGTGGC
TGACGCGCGCCCAGGCGCCATCGTTGCTGCTGGTGGATCTGGAAATGCCGGGCATGGACGGCGTGCAGTTGCTCGACGCC
CTGGCGCGCGGCAAATACAGCGTGCCGGTAGTGGTGGTCTCGCAACGCGGCGGTGCCTTGATCGATGCGGTGATGCAACT
CAGCCGCAGTGCCGGCGTGCGCGTGCTCGGCGGCATCGAAAAGCCGATGAACCTGCAGGATCTGGCCACCGTACTGGAGT
GCGAACCGGAGCCGGCCGCCGACGCACCGGCGTTCGCGCCGGTGGCGATTTCGCCGCTGATGTCCAGCGGTGGCGCCGCC
GCCTCGCGGCTGGATCGCGCCATGCGCCGCGGCGAGATCCGCGTGGCCTACCAACCCAAGCTCGATCTGAAGGACGGCCG
CCTGCGTGGCGTGGAAGCGCTCGCACGTTGGCGCCGCCCGCAAGGCGACATGATCGGCCCGGACCGTTTCATTCCGCTGG
CCGAACGCGAAGGCTTGATCCACGCGTTGACGCAGCAGGTCATCGACAAGGCGATCGCGCAATTGGTGGACTGGCGCGCC
GAAGGCTTCGACCTGAGCCTGGCCCTGAACCTTTCGCCCCGGCTGCTGGGCGAGGACCATTTCCTCGAAGAGCTATGCGC
CAAGCTGGGCGAGAACGGCCTAAGCCCGGCCGATCTAGTACTGGAGCTCACCGAAAGCGCCATCGTGGAGCCGGCCAATG
CGCTGAGCATGCTGGCGCGTTTGCGCCTGCACGGGTTCGGCCTGTCCATCGACGACTACGGCACCGGCTTCTCCTCGCTG
CAGCGCCTGGCCAGCATTCCCTTCACCGAACTCAAGCTGGACCGCAGCTTCGTGCATGCGGCGCATCGCAGCCGCAGCCA
GCGCACCGTGCTCGAATCCACGCTGGAACTGGCGCACCGGCTCGAGCTCACTGCCGTGGCGGAAGGCGTGGAAACGCCCG
ACGACTGGCGGCTGCTGCGCGAACTTGGCTGCGATCTGGCACAAGGCTATCTGATGGCATCGCCGATGCCCGGGCCGATG
CTGTCGGAGTGGTGGCGCGAACACGCCGTGCGTATCGCACGCCTGAGCGACAACACGTTCCACAGCGATGCGGACGTGGC
CTGA

Upstream 100 bases:

>100_bases
TCCTGCTCGATGCCGATGCCGTGCTGGGCAACGACACGCTCGCCCAGCTGCCAGCCGCCGAGCTGGCAGCCTGACCTCAC
GCATGCACTCAAGGACTCCC

Downstream 100 bases:

>100_bases
GCCGATGAGCACCGCCACCGCCATCACCGAACAGGAGTTCGGCCGCTTCCAGCGCTTCATCTTCGAAGCGGCCGGCATCA
CCATTTCCTCCGGCAAGAAG

Product: response regulator

Products: NA

Alternate protein names: Direct oxygen sensing phosphodiesterase; Direct oxygen sensor protein; Ec DOS; Heme-regulated cyclic di-GMP phosphodiesterase, [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MRPALLSCNCTLAGPVLVVDDSVVQREHAMALCRQLGASVVDGAVDGNAALEWLTRAQAPSLLLVDLEMPGMDGVQLLDA
LARGKYSVPVVVVSQRGGALIDAVMQLSRSAGVRVLGGIEKPMNLQDLATVLECEPEPAADAPAFAPVAISPLMSSGGAA
ASRLDRAMRRGEIRVAYQPKLDLKDGRLRGVEALARWRRPQGDMIGPDRFIPLAEREGLIHALTQQVIDKAIAQLVDWRA
EGFDLSLALNLSPRLLGEDHFLEELCAKLGENGLSPADLVLELTESAIVEPANALSMLARLRLHGFGLSIDDYGTGFSSL
QRLASIPFTELKLDRSFVHAAHRSRSQRTVLESTLELAHRLELTAVAEGVETPDDWRLLRELGCDLAQGYLMASPMPGPM
LSEWWREHAVRIARLSDNTFHSDADVA

Sequences:

>Translated_427_residues
MRPALLSCNCTLAGPVLVVDDSVVQREHAMALCRQLGASVVDGAVDGNAALEWLTRAQAPSLLLVDLEMPGMDGVQLLDA
LARGKYSVPVVVVSQRGGALIDAVMQLSRSAGVRVLGGIEKPMNLQDLATVLECEPEPAADAPAFAPVAISPLMSSGGAA
ASRLDRAMRRGEIRVAYQPKLDLKDGRLRGVEALARWRRPQGDMIGPDRFIPLAEREGLIHALTQQVIDKAIAQLVDWRA
EGFDLSLALNLSPRLLGEDHFLEELCAKLGENGLSPADLVLELTESAIVEPANALSMLARLRLHGFGLSIDDYGTGFSSL
QRLASIPFTELKLDRSFVHAAHRSRSQRTVLESTLELAHRLELTAVAEGVETPDDWRLLRELGCDLAQGYLMASPMPGPM
LSEWWREHAVRIARLSDNTFHSDADVA
>Mature_427_residues
MRPALLSCNCTLAGPVLVVDDSVVQREHAMALCRQLGASVVDGAVDGNAALEWLTRAQAPSLLLVDLEMPGMDGVQLLDA
LARGKYSVPVVVVSQRGGALIDAVMQLSRSAGVRVLGGIEKPMNLQDLATVLECEPEPAADAPAFAPVAISPLMSSGGAA
ASRLDRAMRRGEIRVAYQPKLDLKDGRLRGVEALARWRRPQGDMIGPDRFIPLAEREGLIHALTQQVIDKAIAQLVDWRA
EGFDLSLALNLSPRLLGEDHFLEELCAKLGENGLSPADLVLELTESAIVEPANALSMLARLRLHGFGLSIDDYGTGFSSL
QRLASIPFTELKLDRSFVHAAHRSRSQRTVLESTLELAHRLELTAVAEGVETPDDWRLLRELGCDLAQGYLMASPMPGPM
LSEWWREHAVRIARLSDNTFHSDADVA

Specific function: In association with DosC is involved in the production and removal of the second messenger c-di-GMP in response to changing O(2) levels. Has phosphodiesterase (PDE) activity with c- di-GMP (PubMed:15995192), very poor activity on cAMP (PubMed:15995192) bu

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI87081921, Length=355, Percent_Identity=30.7042253521127, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1790496, Length=242, Percent_Identity=33.8842975206612, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1787541, Length=251, Percent_Identity=33.8645418326693, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI87081743, Length=246, Percent_Identity=33.3333333333333, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI87081845, Length=240, Percent_Identity=36.25, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1787055, Length=248, Percent_Identity=34.6774193548387, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1788502, Length=258, Percent_Identity=34.1085271317829, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1786507, Length=241, Percent_Identity=33.6099585062241, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI226510982, Length=245, Percent_Identity=31.0204081632653, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87081980, Length=245, Percent_Identity=32.2448979591837, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1788849, Length=240, Percent_Identity=29.1666666666667, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87082096, Length=233, Percent_Identity=27.8969957081545, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787410, Length=236, Percent_Identity=24.5762711864407, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR012226
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013767 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF00989 PAS; PF08448 PAS_4 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 46273; Mature: 46273

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPALLSCNCTLAGPVLVVDDSVVQREHAMALCRQLGASVVDGAVDGNAALEWLTRAQAP
CCCCEEECCCEECCCEEEECCHHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHCCCC
SLLLVDLEMPGMDGVQLLDALARGKYSVPVVVVSQRGGALIDAVMQLSRSAGVRVLGGIE
CEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEECCC
KPMNLQDLATVLECEPEPAADAPAFAPVAISPLMSSGGAAASRLDRAMRRGEIRVAYQPK
CCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCC
LDLKDGRLRGVEALARWRRPQGDMIGPDRFIPLAEREGLIHALTQQVIDKAIAQLVDWRA
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCC
EGFDLSLALNLSPRLLGEDHFLEELCAKLGENGLSPADLVLELTESAIVEPANALSMLAR
CCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
LRLHGFGLSIDDYGTGFSSLQRLASIPFTELKLDRSFVHAAHRSRSQRTVLESTLELAHR
HHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
LELTAVAEGVETPDDWRLLRELGCDLAQGYLMASPMPGPMLSEWWREHAVRIARLSDNTF
HHHHHHHCCCCCCHHHHHHHHHCCHHHCCEEEECCCCCHHHHHHHHHHHHEEEEECCCCC
HSDADVA
CCCCCCC
>Mature Secondary Structure
MRPALLSCNCTLAGPVLVVDDSVVQREHAMALCRQLGASVVDGAVDGNAALEWLTRAQAP
CCCCEEECCCEECCCEEEECCHHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHCCCC
SLLLVDLEMPGMDGVQLLDALARGKYSVPVVVVSQRGGALIDAVMQLSRSAGVRVLGGIE
CEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEECCC
KPMNLQDLATVLECEPEPAADAPAFAPVAISPLMSSGGAAASRLDRAMRRGEIRVAYQPK
CCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCC
LDLKDGRLRGVEALARWRRPQGDMIGPDRFIPLAEREGLIHALTQQVIDKAIAQLVDWRA
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCC
EGFDLSLALNLSPRLLGEDHFLEELCAKLGENGLSPADLVLELTESAIVEPANALSMLAR
CCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
LRLHGFGLSIDDYGTGFSSLQRLASIPFTELKLDRSFVHAAHRSRSQRTVLESTLELAHR
HHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
LELTAVAEGVETPDDWRLLRELGCDLAQGYLMASPMPGPMLSEWWREHAVRIARLSDNTF
HHHHHHHCCCCCCHHHHHHHHHCCHHHCCEEEECCCCCHHHHHHHHHHHHEEEEECCCCC
HSDADVA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]