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The map label for this gene is rlmH

Identifier: 21243502

GI number: 21243502

Start: 3254054

End: 3254524

Strand: Reverse

Name: rlmH

Synonym: XAC2775

Alternate gene names: 21243502

Gene position: 3254524-3254054 (Counterclockwise)

Preceding gene: 21243504

Following gene: 21243498

Centisome position: 62.88

GC content: 67.52

Gene sequence:

>471_bases
ATGAAATGCCGACTCATCGCTACCGGCGAGCGCGCACCGAGCTGGGTGGCGCAAGGTTTTGCCGAATATCAAAAGCGTCT
TTCGCACTGGATGCCGCTGGAGCTGGTCGAGATCGAACCCGGCATGCGCGGCAAGGGCCGCGATGCGCAGCGCGCGACCG
ACGACGAAGGCCGCCGCGTGCTCGCCGCGCTGCCGAAAAACGCCTACGTGGTGGCGCTTGATGTGCCGGGGCGCCCGCTC
AGTTCCGAGCAGCTCGCCCAGCGCATGGAGCATTGGCGCGGGCAGGGCCGCGACCTGGCGTTTCTCATCGGCGGCCCGGA
AGGCCACGCGGCAGAGGTGCTCAAGAGCGCCAGCGAAAGCTGGTCGATCGGCCCACTCACCCTGCCGCACATGCTGGTGC
GCCTGATCGTCGCCGAGCAGCTGTATCGCGCCGCGGCGATGCTGGCCAACCATCCGTATCACCGCGCATAA

Upstream 100 bases:

>100_bases
GGTGGTCGGGCGACACTAATTGCCAGCTGTAACCACACCATGACACGCAGTGACAAATCGCCGCGGGCTGCCCGGGCTGT
GACGCGGATATCATGTCGCC

Downstream 100 bases:

>100_bases
GAGCGGCTGACGAAACGTGTGTGCAGCGCCATATCGATGCGGCGGCTGCGGTTGCAGGCCGCCGCATCCGGTCGCCGCTC
AATTCAGCGAGAAACTCACC

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKCRLIATGERAPSWVAQGFAEYQKRLSHWMPLELVEIEPGMRGKGRDAQRATDDEGRRVLAALPKNAYVVALDVPGRPL
SSEQLAQRMEHWRGQGRDLAFLIGGPEGHAAEVLKSASESWSIGPLTLPHMLVRLIVAEQLYRAAAMLANHPYHRA

Sequences:

>Translated_156_residues
MKCRLIATGERAPSWVAQGFAEYQKRLSHWMPLELVEIEPGMRGKGRDAQRATDDEGRRVLAALPKNAYVVALDVPGRPL
SSEQLAQRMEHWRGQGRDLAFLIGGPEGHAAEVLKSASESWSIGPLTLPHMLVRLIVAEQLYRAAAMLANHPYHRA
>Mature_156_residues
MKCRLIATGERAPSWVAQGFAEYQKRLSHWMPLELVEIEPGMRGKGRDAQRATDDEGRRVLAALPKNAYVVALDVPGRPL
SSEQLAQRMEHWRGQGRDLAFLIGGPEGHAAEVLKSASESWSIGPLTLPHMLVRLIVAEQLYRAAAMLANHPYHRA

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=155, Percent_Identity=50.9677419354839, Blast_Score=163, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_XANAC (Q8PIX0)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643084.1
- ProteinModelPortal:   Q8PIX0
- SMR:   Q8PIX0
- GeneID:   1156846
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC2775
- NMPDR:   fig|190486.1.peg.2728
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- ProtClustDB:   PRK00103
- BioCyc:   XAXO190486:XAC2775-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17357; Mature: 17357

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCRLIATGERAPSWVAQGFAEYQKRLSHWMPLELVEIEPGMRGKGRDAQRATDDEGRRV
CCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCEE
LAALPKNAYVVALDVPGRPLSSEQLAQRMEHWRGQGRDLAFLIGGPEGHAAEVLKSASES
EEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCC
WSIGPLTLPHMLVRLIVAEQLYRAAAMLANHPYHRA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKCRLIATGERAPSWVAQGFAEYQKRLSHWMPLELVEIEPGMRGKGRDAQRATDDEGRRV
CCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCEE
LAALPKNAYVVALDVPGRPLSSEQLAQRMEHWRGQGRDLAFLIGGPEGHAAEVLKSASES
EEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCC
WSIGPLTLPHMLVRLIVAEQLYRAAAMLANHPYHRA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217