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The map label for this gene is pmbA [H]

Identifier: 21243492

GI number: 21243492

Start: 3237915

End: 3239282

Strand: Reverse

Name: pmbA [H]

Synonym: XAC2765

Alternate gene names: 21243492

Gene position: 3239282-3237915 (Counterclockwise)

Preceding gene: 21243494

Following gene: 21243491

Centisome position: 62.59

GC content: 68.86

Gene sequence:

>1368_bases
TTGAACGTACTCTCCTCCGAAATCCGCGTCACCGACGACAGCCTGCAGCGCCTGGATGCGCTCACCGACATCTCCCAGCG
CCTGCTCGAGCGCGCGCGCGCGGCCGGCGCCACGCAGGCCGAAGTGAGCTGCAGCGAAGAGCGCGGCCTGGACGTCAATG
TGCGGCTGGGCGATGTGGAGACGGTCGAATCCACGCGCGATCGCGGCATTGCGGTCACGGTCTACTTCGGCAAGCGCAAG
GGCAGTGCCAGCACCGCCGACCTGCAGGAGTCCAGCCTGGAATCCACCGTGGCGCAGGCCTGCGCGATCGCCCGCTTCAC
CGAGGACGACGTGGCCGCCGGCCTGGCCGACCCGGAGTTGATGGCGCGCGAGTTCCCCGAGCTGGATAGCTGGCACCCGT
GGGCGCTGGACGCCGACACCGCCGTGGATCTGGCGCTGGCCTGCGAAACCGCCGGCCGCGACGCCGATGCGCGCATCAGC
AACTCCGACGGCGCCTCGGCCAGCACCGGGCTCAGCCTGTCGGTGTACGCCAATTCGCATGGCTTCGTCGGCCGCGAGCG
CGGTACCCATCATTCGATCAGCTGTGCGCTGATCGCCGGCCAGGGCGACGGCATGCAGCGCGACGGCTGGTACAGCAGTG
CACTGGCACGCGAGGACCTGGAAGCGCCCGACGCCATCGGCCGCCATGCCGCCGCGCGTACCGTGGCGCGCTTGCAGCCG
CGTTCGCTGCCCACCGGCCAGTTGCCGGTGCTGTTCGCTCCGGAAGTGGCGCGCTCGCTGGTCGGCCACCTGCTCGGTGC
GGTATCCGGCGGCGCGCTGTATCGCCGCGCCAGCTTCCTGCTCGACAGCGTCGGCAGCAAGTTGTTCCCGGACTGGTTCA
ACATCGAAGAGCTGCCGCACCTGCGCCGCGGCTTGCGCTCGGCCGCCTTCGACGGCGAAGGCGTGGCGACGCGGCGTTCG
GCGCTGGTCACCGACGGCGTGCTGCAGCGCTATGTGCTGGGCAGCTATTCGGCACGCAAGCTGGGCCTGCAGACCACCGC
CAATGCCGGCGGCGTCCACAACCTGCAGGTCACCGCCAATGCAGGCGATCTGGCGTCGATGATTGCCGGCATGTCGCGCG
GCCTGCTGGTCACCGAATTGATGGGCAATGGCGTCAATCCCGTCACCGGCGACTATTCGCGCGGGGCTGGTGGCTTCTGG
ATCGAGAATGGCGTGATTGCCTATCCGGTCGATGGCCTGACCATCGCCGGCAATCTGCGCGACATGTTCGCCGGCATCGA
AGCGGTGGGCAGCGATGTCGATCCGCGCTCGCACGTCAAGATCGGCTCGGTGCTGATCAACCGCATGACGGTGGCAGGCG
ACAGCTGA

Upstream 100 bases:

>100_bases
CGGCCGGGCGTCGGCGGAACAACTCCGCACCGAATTCAAACACTCCGGCCATGGACAGCCGGGCTTTCGCGGACGGATCC
GCGTTTCCAAGGATAAAGCA

Downstream 100 bases:

>100_bases
GCGGCATCGGCTGTACCGGCTTCACCGGTTTCCGAAGCGGCGGCTTGTTGTGTCGGCGCACGTCGCGCGAGCAGTCGTTC
GAGCCTGACGGTGCATGCGC

Product: PmbA protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MNVLSSEIRVTDDSLQRLDALTDISQRLLERARAAGATQAEVSCSEERGLDVNVRLGDVETVESTRDRGIAVTVYFGKRK
GSASTADLQESSLESTVAQACAIARFTEDDVAAGLADPELMAREFPELDSWHPWALDADTAVDLALACETAGRDADARIS
NSDGASASTGLSLSVYANSHGFVGRERGTHHSISCALIAGQGDGMQRDGWYSSALAREDLEAPDAIGRHAAARTVARLQP
RSLPTGQLPVLFAPEVARSLVGHLLGAVSGGALYRRASFLLDSVGSKLFPDWFNIEELPHLRRGLRSAAFDGEGVATRRS
ALVTDGVLQRYVLGSYSARKLGLQTTANAGGVHNLQVTANAGDLASMIAGMSRGLLVTELMGNGVNPVTGDYSRGAGGFW
IENGVIAYPVDGLTIAGNLRDMFAGIEAVGSDVDPRSHVKIGSVLINRMTVAGDS

Sequences:

>Translated_455_residues
MNVLSSEIRVTDDSLQRLDALTDISQRLLERARAAGATQAEVSCSEERGLDVNVRLGDVETVESTRDRGIAVTVYFGKRK
GSASTADLQESSLESTVAQACAIARFTEDDVAAGLADPELMAREFPELDSWHPWALDADTAVDLALACETAGRDADARIS
NSDGASASTGLSLSVYANSHGFVGRERGTHHSISCALIAGQGDGMQRDGWYSSALAREDLEAPDAIGRHAAARTVARLQP
RSLPTGQLPVLFAPEVARSLVGHLLGAVSGGALYRRASFLLDSVGSKLFPDWFNIEELPHLRRGLRSAAFDGEGVATRRS
ALVTDGVLQRYVLGSYSARKLGLQTTANAGGVHNLQVTANAGDLASMIAGMSRGLLVTELMGNGVNPVTGDYSRGAGGFW
IENGVIAYPVDGLTIAGNLRDMFAGIEAVGSDVDPRSHVKIGSVLINRMTVAGDS
>Mature_455_residues
MNVLSSEIRVTDDSLQRLDALTDISQRLLERARAAGATQAEVSCSEERGLDVNVRLGDVETVESTRDRGIAVTVYFGKRK
GSASTADLQESSLESTVAQACAIARFTEDDVAAGLADPELMAREFPELDSWHPWALDADTAVDLALACETAGRDADARIS
NSDGASASTGLSLSVYANSHGFVGRERGTHHSISCALIAGQGDGMQRDGWYSSALAREDLEAPDAIGRHAAARTVARLQP
RSLPTGQLPVLFAPEVARSLVGHLLGAVSGGALYRRASFLLDSVGSKLFPDWFNIEELPHLRRGLRSAAFDGEGVATRRS
ALVTDGVLQRYVLGSYSARKLGLQTTANAGGVHNLQVTANAGDLASMIAGMSRGLLVTELMGNGVNPVTGDYSRGAGGFW
IENGVIAYPVDGLTIAGNLRDMFAGIEAVGSDVDPRSHVKIGSVLINRMTVAGDS

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=414, Percent_Identity=47.8260869565217, Blast_Score=405, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47981; Mature: 47981

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLSSEIRVTDDSLQRLDALTDISQRLLERARAAGATQAEVSCSEERGLDVNVRLGDVE
CCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEECCCH
TVESTRDRGIAVTVYFGKRKGSASTADLQESSLESTVAQACAIARFTEDDVAAGLADPEL
HHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHH
MAREFPELDSWHPWALDADTAVDLALACETAGRDADARISNSDGASASTGLSLSVYANSH
HHHHCCCCCCCCCCEECCCHHHHHHEEECCCCCCCCCEECCCCCCCCCCCEEEEEEECCC
GFVGRERGTHHSISCALIAGQGDGMQRDGWYSSALAREDLEAPDAIGRHAAARTVARLQP
CCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
RSLPTGQLPVLFAPEVARSLVGHLLGAVSGGALYRRASFLLDSVGSKLFPDWFNIEELPH
CCCCCCCCCEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LRRGLRSAAFDGEGVATRRSALVTDGVLQRYVLGSYSARKLGLQTTANAGGVHNLQVTAN
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCEECCCCCCEEEEEEECC
AGDLASMIAGMSRGLLVTELMGNGVNPVTGDYSRGAGGFWIENGVIAYPVDGLTIAGNLR
CHHHHHHHHHHCCCEEHHHHHCCCCCCCCCCCCCCCCCEEEECCEEEECCCCEEEECCHH
DMFAGIEAVGSDVDPRSHVKIGSVLINRMTVAGDS
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHEECCCC
>Mature Secondary Structure
MNVLSSEIRVTDDSLQRLDALTDISQRLLERARAAGATQAEVSCSEERGLDVNVRLGDVE
CCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEECCCH
TVESTRDRGIAVTVYFGKRKGSASTADLQESSLESTVAQACAIARFTEDDVAAGLADPEL
HHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHH
MAREFPELDSWHPWALDADTAVDLALACETAGRDADARISNSDGASASTGLSLSVYANSH
HHHHCCCCCCCCCCEECCCHHHHHHEEECCCCCCCCCEECCCCCCCCCCCEEEEEEECCC
GFVGRERGTHHSISCALIAGQGDGMQRDGWYSSALAREDLEAPDAIGRHAAARTVARLQP
CCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
RSLPTGQLPVLFAPEVARSLVGHLLGAVSGGALYRRASFLLDSVGSKLFPDWFNIEELPH
CCCCCCCCCEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LRRGLRSAAFDGEGVATRRSALVTDGVLQRYVLGSYSARKLGLQTTANAGGVHNLQVTAN
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCEECCCCCCEEEEEEECC
AGDLASMIAGMSRGLLVTELMGNGVNPVTGDYSRGAGGFWIENGVIAYPVDGLTIAGNLR
CHHHHHHHHHHCCCEEHHHHHCCCCCCCCCCCCCCCCCEEEECCEEEECCCCEEEECCHH
DMFAGIEAVGSDVDPRSHVKIGSVLINRMTVAGDS
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]