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The map label for this gene is rimO [H]

Identifier: 21243462

GI number: 21243462

Start: 3198750

End: 3200123

Strand: Reverse

Name: rimO [H]

Synonym: XAC2735

Alternate gene names: 21243462

Gene position: 3200123-3198750 (Counterclockwise)

Preceding gene: 21243467

Following gene: 77748671

Centisome position: 61.83

GC content: 63.1

Gene sequence:

>1374_bases
ATGTCCCAGCTGAACCCTAAAGTCGGCTTCGTCAGCCTTGGCTGCCCGAAGGCGCTCGTCGATTCCGAACGCATCCTCAC
CCAGCTGCGCGTAGAGGGCTACGACATCGTGCCCAGCTACGACGCTGCCGATGTGGTGGTGGTCAACACCTGCGGCTTCA
TCGATTCGGCAGTGACCGAATCGCTGGACGCGATCGGCGAGGCGATGAACGCCAACGGCAAGGTCATCGTCACCGGCTGC
CTGGGCAAGCGCCCGGAGCAGATTCGCGAGGCGTACCCGCAAGTGCTGGCGGTATCCGGCCCGCAGGACTACCAGAGCGT
CATGGAGGCAGTGCATGCCGCGTTGCCGCCGCGTCACGACCCGTTCGTGGATCTGGTGCCGGACTACGGCATCAAGCTGA
CGCCGCGCCATTACGCGTATCTCAAGATTTCCGAAGGCTGCAATCACCGGTGCAGCTTCTGCATCATTCCCTCGATGCGC
GGCGATCTGGTCTCGCGTCCGGTCGATGAAGTGCTGCGCGAAGCCGAGCGGCTGGTGCGCGGTGGCGTCAAGGAATTGCT
GGTGGTGTCGCAGGACACCTCGGCCTACGGTGTGGACCTGAAATATGCCGAGCGTCCGTGGCGGGACCGCCTGTACCAGA
CCCGCATGAAGGCGCTGTGCGAGGGGTTGTCGGAGCTTGGCGTGTGGACGCGCCTGCATTACGTGTACCCGTATCCGCAC
GTGGACGATGTGATTGGGCTGATGGCCGAAGGCAAGCTGCTGCCGTATCTGGACATTCCGTTCCAGCACGCCAGCCCGCG
CATTCTCAAGTTGATGAAGCGCCCCGGCGCGGTGGAAAAAACCCTGGAGCGCGTGCAGCGCTGGAAGGCGATGTGCCCGG
AGATCACCGTGCGCTCGACCTTCATCGTCGGCTTCCCCGGCGAGACCGATGCCGAATTCGAATCCTTGCTGGACTTCTTG
GATCAGGCGCAGCTGGACCGCGTCGGTGCGTTTGCGTATTCGCCGGTCGATGGCGCCAGCGCCAACGCGCTGCCGGATCA
GGTGCCCGAAGAAGTGAAACAGGAGCGCCTGGCGCGCTTCATGGCCAAGCAGGCGCAGATCTCTGCGTTGCGTCTGGAAT
CCAAGATCGGCAGCGTGCAGCAGTGCCTGGTGGATGTGATCGAAGACGACATCGCGGTGGCCCGCTCGCGTGCCGATGCG
CCTGAAATCGATGGGCTGGTGCACATCCAGAACGGTGGCGAGCTCGGCTTGAAGGTGGGCGATCTGGTGGATGTTGAAAT
CACCGACAGCGACGAGCACGATCTGTTTGGCGATGCACTGCCGTCTGGCCATGCGGTGCAGCCAGGGCGGACATTGAACC
TGCAGATCGTTTAG

Upstream 100 bases:

>100_bases
CCCCCGCGTGCGCGGCATGCGGTTCAGAGCGCCGCCCCCACACCGCTATAATTGGCCCGCTCAGGCCCCAGGCCGCTCCG
CTTCCCAGGCTCCTGCTTCC

Downstream 100 bases:

>100_bases
CCGCAGCAGGGCGCCGGCTGGCCGATGGCGTCGGCGCCAGACTGTGCCGCACCGTCGCCGCACATGCCCCGGTGGGCACG
CCAACCGGTAGGAGCGTGCC

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSQLNPKVGFVSLGCPKALVDSERILTQLRVEGYDIVPSYDAADVVVVNTCGFIDSAVTESLDAIGEAMNANGKVIVTGC
LGKRPEQIREAYPQVLAVSGPQDYQSVMEAVHAALPPRHDPFVDLVPDYGIKLTPRHYAYLKISEGCNHRCSFCIIPSMR
GDLVSRPVDEVLREAERLVRGGVKELLVVSQDTSAYGVDLKYAERPWRDRLYQTRMKALCEGLSELGVWTRLHYVYPYPH
VDDVIGLMAEGKLLPYLDIPFQHASPRILKLMKRPGAVEKTLERVQRWKAMCPEITVRSTFIVGFPGETDAEFESLLDFL
DQAQLDRVGAFAYSPVDGASANALPDQVPEEVKQERLARFMAKQAQISALRLESKIGSVQQCLVDVIEDDIAVARSRADA
PEIDGLVHIQNGGELGLKVGDLVDVEITDSDEHDLFGDALPSGHAVQPGRTLNLQIV

Sequences:

>Translated_457_residues
MSQLNPKVGFVSLGCPKALVDSERILTQLRVEGYDIVPSYDAADVVVVNTCGFIDSAVTESLDAIGEAMNANGKVIVTGC
LGKRPEQIREAYPQVLAVSGPQDYQSVMEAVHAALPPRHDPFVDLVPDYGIKLTPRHYAYLKISEGCNHRCSFCIIPSMR
GDLVSRPVDEVLREAERLVRGGVKELLVVSQDTSAYGVDLKYAERPWRDRLYQTRMKALCEGLSELGVWTRLHYVYPYPH
VDDVIGLMAEGKLLPYLDIPFQHASPRILKLMKRPGAVEKTLERVQRWKAMCPEITVRSTFIVGFPGETDAEFESLLDFL
DQAQLDRVGAFAYSPVDGASANALPDQVPEEVKQERLARFMAKQAQISALRLESKIGSVQQCLVDVIEDDIAVARSRADA
PEIDGLVHIQNGGELGLKVGDLVDVEITDSDEHDLFGDALPSGHAVQPGRTLNLQIV
>Mature_456_residues
SQLNPKVGFVSLGCPKALVDSERILTQLRVEGYDIVPSYDAADVVVVNTCGFIDSAVTESLDAIGEAMNANGKVIVTGCL
GKRPEQIREAYPQVLAVSGPQDYQSVMEAVHAALPPRHDPFVDLVPDYGIKLTPRHYAYLKISEGCNHRCSFCIIPSMRG
DLVSRPVDEVLREAERLVRGGVKELLVVSQDTSAYGVDLKYAERPWRDRLYQTRMKALCEGLSELGVWTRLHYVYPYPHV
DDVIGLMAEGKLLPYLDIPFQHASPRILKLMKRPGAVEKTLERVQRWKAMCPEITVRSTFIVGFPGETDAEFESLLDFLD
QAQLDRVGAFAYSPVDGASANALPDQVPEEVKQERLARFMAKQAQISALRLESKIGSVQQCLVDVIEDDIAVARSRADAP
EIDGLVHIQNGGELGLKVGDLVDVEITDSDEHDLFGDALPSGHAVQPGRTLNLQIV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=465, Percent_Identity=26.0215053763441, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=25.5230125523013, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=25.5230125523013, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1787057, Length=434, Percent_Identity=67.2811059907834, Blast_Score=618, Evalue=1e-178,
Organism=Escherichia coli, GI1786882, Length=455, Percent_Identity=28.1318681318681, Blast_Score=161, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17553146, Length=462, Percent_Identity=25.3246753246753, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI71996771, Length=253, Percent_Identity=26.8774703557312, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356207, Length=482, Percent_Identity=25.5186721991701, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19922432, Length=374, Percent_Identity=24.8663101604278, Blast_Score=99, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50404; Mature: 50273

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLNPKVGFVSLGCPKALVDSERILTQLRVEGYDIVPSYDAADVVVVNTCGFIDSAVTE
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCEEEEECCCHHHHHHHH
SLDAIGEAMNANGKVIVTGCLGKRPEQIREAYPQVLAVSGPQDYQSVMEAVHAALPPRHD
HHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCC
PFVDLVPDYGIKLTPRHYAYLKISEGCNHRCSFCIIPSMRGDLVSRPVDEVLREAERLVR
CCEEECCCCCCEECCCEEEEEEECCCCCCCCCEEEECCCCCHHHCCCHHHHHHHHHHHHH
GGVKELLVVSQDTSAYGVDLKYAERPWRDRLYQTRMKALCEGLSELGVWTRLHYVYPYPH
CCHHHEEEEECCCCCCCEEEHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCCC
VDDVIGLMAEGKLLPYLDIPFQHASPRILKLMKRPGAVEKTLERVQRWKAMCPEITVRST
HHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEE
FIVGFPGETDAEFESLLDFLDQAQLDRVGAFAYSPVDGASANALPDQVPEEVKQERLARF
EEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
MAKQAQISALRLESKIGSVQQCLVDVIEDDIAVARSRADAPEIDGLVHIQNGGELGLKVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEC
DLVDVEITDSDEHDLFGDALPSGHAVQPGRTLNLQIV
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure 
SQLNPKVGFVSLGCPKALVDSERILTQLRVEGYDIVPSYDAADVVVVNTCGFIDSAVTE
CCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCEEEEECCCHHHHHHHH
SLDAIGEAMNANGKVIVTGCLGKRPEQIREAYPQVLAVSGPQDYQSVMEAVHAALPPRHD
HHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCC
PFVDLVPDYGIKLTPRHYAYLKISEGCNHRCSFCIIPSMRGDLVSRPVDEVLREAERLVR
CCEEECCCCCCEECCCEEEEEEECCCCCCCCCEEEECCCCCHHHCCCHHHHHHHHHHHHH
GGVKELLVVSQDTSAYGVDLKYAERPWRDRLYQTRMKALCEGLSELGVWTRLHYVYPYPH
CCHHHEEEEECCCCCCCEEEHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCCC
VDDVIGLMAEGKLLPYLDIPFQHASPRILKLMKRPGAVEKTLERVQRWKAMCPEITVRST
HHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEE
FIVGFPGETDAEFESLLDFLDQAQLDRVGAFAYSPVDGASANALPDQVPEEVKQERLARF
EEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
MAKQAQISALRLESKIGSVQQCLVDVIEDDIAVARSRADAPEIDGLVHIQNGGELGLKVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEC
DLVDVEITDSDEHDLFGDALPSGHAVQPGRTLNLQIV
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA