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The map label for this gene is trpI [H]

Identifier: 21243445

GI number: 21243445

Start: 3178717

End: 3179643

Strand: Direct

Name: trpI [H]

Synonym: XAC2718

Alternate gene names: 21243445

Gene position: 3178717-3179643 (Clockwise)

Preceding gene: 21243435

Following gene: 21243454

Centisome position: 61.42

GC content: 69.15

Gene sequence:

>927_bases
ATGAATGCTTCGCTTCCCCCGTTGGCGGCGCTGCGCGCGTTCGAAGCTGCCGCGCGCTTGCAGAGCGTCAGCCGTGCTGC
CCAGGAACTGCACGTCACCCATGGCGCCGTCAGTCGGCATGTGCGTTCGCTGGAACAGGCATTGGGCACCGCGCTGTTTG
CGCGCCAGGGCCGCGGCCTGATGCTGACCGCTGCAGGGGTTCGGCTTCGCGATGCCACCGGCGAAGGCTTTGGCGTGATT
GGTCAGGCCTGGGCGGCATTACAACGCCCGGCCGCCGGAGCGCCGCTGGTGCTGGGATGTTCGGGCAGCTTGTTGGCGCG
CTGGGTGATTCCGCGCCTGGGACTGCTGCAGCAAGAGCTGCCGGACGTGCGCCTGCATCTGTCGGCCAGCGAACAGCCGC
CTGGACCGGACCTGGACGGTCTGGATGCCGCCCTGCTGCTGGACACCCCACCCTGGCCGGGCGAATGGCAGGTGCACGCG
CTGGGCGTGGAGTGGATTGGGCCGGTGCTGAGTCCGCAGCTTGCATCGACCTTGCAGATCGAGGGGAGCGACCCGGCCGT
GCTCGGCGATCACGCGCTGCTGCACACCCGCTCGCGCCCGCAGGCCTGGCCGGAATGGGCACAGGCGCACGGGCTGGCAC
CTGAGGCGCTGCGCTTCGGCACCGAATTCGAGCACCTGGTGTATCTGCTGGAAGCCGCTGCCGCTGGCCTGGGGATCGCG
ATTGCGCCGCAGCCGTTGGTCGCCGCGGACCTGGCAGCCGGGCGCCTGCTGGCGCCGTGGGGATTCGCCGCCACGCATGG
CCGCTGGGTATTGTGCGCCGCCAAGCGCAACCCCGATCCACGCATCGAACGGTTGGCCGGCTGGCTGGGTAGAACACTGG
CGACAGACCAGCACACCTCTTCGACCATGAATGTGGCGAAGAACTGA

Upstream 100 bases:

>100_bases
TCCTCTGCAGCAATGCGTCATACGAGAGGCATCACTCTAGCGCAGCCGCGGCATGGGATAAATCGATAAGATTGCTGCAA
CCTGTGACCTGGAATCACAT

Downstream 100 bases:

>100_bases
GCCACCATCGCGCGCGATCGCCACATGACCACATCACCAATAGCAGGGGATTGCGAACACACGCAGGGAGGAAGCGACTG
CCCAGTGGACCCAGACGCAG

Product: transcriptional regulator

Products: NA

Alternate protein names: TrpBA operon transcriptional activator [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MNASLPPLAALRAFEAAARLQSVSRAAQELHVTHGAVSRHVRSLEQALGTALFARQGRGLMLTAAGVRLRDATGEGFGVI
GQAWAALQRPAAGAPLVLGCSGSLLARWVIPRLGLLQQELPDVRLHLSASEQPPGPDLDGLDAALLLDTPPWPGEWQVHA
LGVEWIGPVLSPQLASTLQIEGSDPAVLGDHALLHTRSRPQAWPEWAQAHGLAPEALRFGTEFEHLVYLLEAAAAGLGIA
IAPQPLVAADLAAGRLLAPWGFAATHGRWVLCAAKRNPDPRIERLAGWLGRTLATDQHTSSTMNVAKN

Sequences:

>Translated_308_residues
MNASLPPLAALRAFEAAARLQSVSRAAQELHVTHGAVSRHVRSLEQALGTALFARQGRGLMLTAAGVRLRDATGEGFGVI
GQAWAALQRPAAGAPLVLGCSGSLLARWVIPRLGLLQQELPDVRLHLSASEQPPGPDLDGLDAALLLDTPPWPGEWQVHA
LGVEWIGPVLSPQLASTLQIEGSDPAVLGDHALLHTRSRPQAWPEWAQAHGLAPEALRFGTEFEHLVYLLEAAAAGLGIA
IAPQPLVAADLAAGRLLAPWGFAATHGRWVLCAAKRNPDPRIERLAGWLGRTLATDQHTSSTMNVAKN
>Mature_308_residues
MNASLPPLAALRAFEAAARLQSVSRAAQELHVTHGAVSRHVRSLEQALGTALFARQGRGLMLTAAGVRLRDATGEGFGVI
GQAWAALQRPAAGAPLVLGCSGSLLARWVIPRLGLLQQELPDVRLHLSASEQPPGPDLDGLDAALLLDTPPWPGEWQVHA
LGVEWIGPVLSPQLASTLQIEGSDPAVLGDHALLHTRSRPQAWPEWAQAHGLAPEALRFGTEFEHLVYLLEAAAAGLGIA
IAPQPLVAADLAAGRLLAPWGFAATHGRWVLCAAKRNPDPRIERLAGWLGRTLATDQHTSSTMNVAKN

Specific function: Activates the expression of the trpBA genes encoding the two tryptophan synthase subunits. In the absence of the inducer (indoleglycerol phosphate), trpI binds upstream of the trpAB operon, overlapping its own promoter region [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=299, Percent_Identity=32.4414715719064, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1786448, Length=264, Percent_Identity=31.0606060606061, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=28.6195286195286, Blast_Score=100, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32622; Mature: 32622

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNASLPPLAALRAFEAAARLQSVSRAAQELHVTHGAVSRHVRSLEQALGTALFARQGRGL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
MLTAAGVRLRDATGEGFGVIGQAWAALQRPAAGAPLVLGCSGSLLARWVIPRLGLLQQEL
EEEECCEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHC
PDVRLHLSASEQPPGPDLDGLDAALLLDTPPWPGEWQVHALGVEWIGPVLSPQLASTLQI
CCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECHHHHHHHCCHHHHHEEEE
EGSDPAVLGDHALLHTRSRPQAWPEWAQAHGLAPEALRFGTEFEHLVYLLEAAAAGLGIA
CCCCCCEECCCHHEECCCCCCCCHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHCCCCEE
IAPQPLVAADLAAGRLLAPWGFAATHGRWVLCAAKRNPDPRIERLAGWLGRTLATDQHTS
ECCCCHHHHHHCCCCEECCCCCEECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
STMNVAKN
HHHHHCCC
>Mature Secondary Structure
MNASLPPLAALRAFEAAARLQSVSRAAQELHVTHGAVSRHVRSLEQALGTALFARQGRGL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
MLTAAGVRLRDATGEGFGVIGQAWAALQRPAAGAPLVLGCSGSLLARWVIPRLGLLQQEL
EEEECCEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHC
PDVRLHLSASEQPPGPDLDGLDAALLLDTPPWPGEWQVHALGVEWIGPVLSPQLASTLQI
CCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECHHHHHHHCCHHHHHEEEE
EGSDPAVLGDHALLHTRSRPQAWPEWAQAHGLAPEALRFGTEFEHLVYLLEAAAAGLGIA
CCCCCCEECCCHHEECCCCCCCCHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHCCCCEE
IAPQPLVAADLAAGRLLAPWGFAATHGRWVLCAAKRNPDPRIERLAGWLGRTLATDQHTS
ECCCCHHHHHHCCCCEECCCCCEECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
STMNVAKN
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8423001 [H]