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The map label for this gene is trpA

Identifier: 21243443

GI number: 21243443

Start: 3176119

End: 3176925

Strand: Reverse

Name: trpA

Synonym: XAC2716

Alternate gene names: 21243443

Gene position: 3176925-3176119 (Counterclockwise)

Preceding gene: 21243444

Following gene: 21243442

Centisome position: 61.38

GC content: 69.14

Gene sequence:

>807_bases
ATGCGTCGTATCGATTCCTGCTTCGCCGAGCTGCGTGCCAATGGGCGCAAGGCACTGATCCCTTTCATCACCGCCGGCGA
CCCATCGTTGGAAGCCACCGTGCCGGTGATGCACGCGCTCGTGCGCGCCGGTGCCGATGTGATCGAGCTGGGTGTGCCGT
TCTCCGACCCGATGGCCGACGGCCCCACCATCCAGCGCAGCTCCGAGCGCGCGCTGGCTCGTGGCGCTGGCCTGGCGTAT
GTGCTGGAAGCGGTGCACGAGTTCCGCCGCGAAGATGCCACCACCCCGGTGGTGTTGATGGGCTACCTCAACCCGATCGA
GATCCATGGCACGCGACGCTTCGCCGAGGCCGCGGTCGCCGCGGGCGTGGATGGCGTGCTGCTGGTCGACCTGCCGCCGG
AGGAAGCCGGCGAGACGCGCACCATCTTCACCGAGGTCGGCTTGGCGCTGATCGCACTGGCCTCGCCCACCACCAGCGAG
CAGCGCCTGGATATGTTGTGCAGCACCGCGCAGGGCTATCTGTACTACGTCAGCTTTGCCGGCGTCACCGGCGCCTCCAA
TCTGCTCGACACCCACGCCGCCAGCGATCGCCTGCGCCAGCTGCGTCGGCGTGCCGGCGCGCCCGTGGTGGCCGGCTTCG
GCATCAAGGATGCGGCCAGCGCCGCTGCCATGGCCGTGGATGCCGACGGCGTGGTGGTGGGCAGTGCCCTGGTCGCTGCT
TTGGCGGAAGTGAACGACGTGCGTAGCGCCCGTGAACGCGCCGAAGCGTTCCTGGCCCCCTTGCGCCAGGCGCTCGACCA
GGGATGA

Upstream 100 bases:

>100_bases
TACGGCTACCTGTTTGCGCTGTGCTTTGCGCTGATGCGGTATTTCTTTGCCGCCTGAGGTCAGATGGCGGCCGGCTTTCC
TCCCTTGTTCGAGTGATTTC

Downstream 100 bases:

>100_bases
GAACCGAGCGTCTCTCCCAGCGCTAGAGGGATTGAGGCGAAGGGGCGGGCGCAGGCGTAGCCAGTGCCTGCGCCGCATCG
CGCCACGGCAGCCGCCGCGA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MRRIDSCFAELRANGRKALIPFITAGDPSLEATVPVMHALVRAGADVIELGVPFSDPMADGPTIQRSSERALARGAGLAY
VLEAVHEFRREDATTPVVLMGYLNPIEIHGTRRFAEAAVAAGVDGVLLVDLPPEEAGETRTIFTEVGLALIALASPTTSE
QRLDMLCSTAQGYLYYVSFAGVTGASNLLDTHAASDRLRQLRRRAGAPVVAGFGIKDAASAAAMAVDADGVVVGSALVAA
LAEVNDVRSARERAEAFLAPLRQALDQG

Sequences:

>Translated_268_residues
MRRIDSCFAELRANGRKALIPFITAGDPSLEATVPVMHALVRAGADVIELGVPFSDPMADGPTIQRSSERALARGAGLAY
VLEAVHEFRREDATTPVVLMGYLNPIEIHGTRRFAEAAVAAGVDGVLLVDLPPEEAGETRTIFTEVGLALIALASPTTSE
QRLDMLCSTAQGYLYYVSFAGVTGASNLLDTHAASDRLRQLRRRAGAPVVAGFGIKDAASAAAMAVDADGVVVGSALVAA
LAEVNDVRSARERAEAFLAPLRQALDQG
>Mature_268_residues
MRRIDSCFAELRANGRKALIPFITAGDPSLEATVPVMHALVRAGADVIELGVPFSDPMADGPTIQRSSERALARGAGLAY
VLEAVHEFRREDATTPVVLMGYLNPIEIHGTRRFAEAAVAAGVDGVLLVDLPPEEAGETRTIFTEVGLALIALASPTTSE
QRLDMLCSTAQGYLYYVSFAGVTGASNLLDTHAASDRLRQLRRRAGAPVVAGFGIKDAASAAAMAVDADGVVVGSALVAA
LAEVNDVRSARERAEAFLAPLRQALDQG

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=269, Percent_Identity=34.2007434944238, Blast_Score=147, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6321412, Length=239, Percent_Identity=41.0041841004184, Blast_Score=191, Evalue=1e-49,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_XANAC (Q8PJ29)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643025.1
- ProteinModelPortal:   Q8PJ29
- SMR:   Q8PJ29
- GeneID:   1156787
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC2716
- NMPDR:   fig|190486.1.peg.2669
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   XAXO190486:XAC2716-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28153; Mature: 28153

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIDSCFAELRANGRKALIPFITAGDPSLEATVPVMHALVRAGADVIELGVPFSDPMAD
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPTIQRSSERALARGAGLAYVLEAVHEFRREDATTPVVLMGYLNPIEIHGTRRFAEAAVA
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCHHHHHHHHHH
AGVDGVLLVDLPPEEAGETRTIFTEVGLALIALASPTTSEQRLDMLCSTAQGYLYYVSFA
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEEC
GVTGASNLLDTHAASDRLRQLRRRAGAPVVAGFGIKDAASAAAMAVDADGVVVGSALVAA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHEEECCCCCHHHHHHHHH
LAEVNDVRSARERAEAFLAPLRQALDQG
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRRIDSCFAELRANGRKALIPFITAGDPSLEATVPVMHALVRAGADVIELGVPFSDPMAD
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPTIQRSSERALARGAGLAYVLEAVHEFRREDATTPVVLMGYLNPIEIHGTRRFAEAAVA
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCHHHHHHHHHH
AGVDGVLLVDLPPEEAGETRTIFTEVGLALIALASPTTSEQRLDMLCSTAQGYLYYVSFA
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEEC
GVTGASNLLDTHAASDRLRQLRRRAGAPVVAGFGIKDAASAAAMAVDADGVVVGSALVAA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHEEECCCCCHHHHHHHHH
LAEVNDVRSARERAEAFLAPLRQALDQG
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217