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The map label for this gene is insF1 [C]

Identifier: 21243390

GI number: 21243390

Start: 3119656

End: 3120462

Strand: Reverse

Name: insF1 [C]

Synonym: XAC2661

Alternate gene names: 21243390

Gene position: 3120462-3119656 (Counterclockwise)

Preceding gene: 21243391

Following gene: 21243383

Centisome position: 60.29

GC content: 62.7

Gene sequence:

>807_bases
ATGTGCCGGGTTTTGCGGGTGAACCGGGCTGGATACTACGCGTGGCTAAAGTCGCCCGACAGTGAACGCGCCAAGGAAGA
CGAACGCCTGCTGGGGCTGATCAAGCACCACTGGTTGGCCAGCGGCAGTGTCTATGGGCATCGCAAGATTGCCAAGGATC
TACGCGATCTGGGTGAGCGTTGCAGTCGCCATCGGGTGCATCGATTGATGCGCACCGAGGGACTGCGTGCCCAGGTGGGC
TATGGCCGCAAGCCGCGCTTCCATGGCGGAACGCCGTGCAAGGCGGCAGCCAACCTGCTGGATCGACAGTTCGACGTGAC
CGAGCCGGACACGGCCTGGGCGAGCGACTTCACCTTCATCCGTACGCATGAAGGCTGGATGTACCTGGCTGTGGTGATCG
ATCTGTTTTCCAGGCAGGTCGTCGGCTGGGCGATGCGCGATCGAGCCGATACCGAGTTGGTCGTGCAGGCCTTGCTGTCG
GCGGTGTGGCGGCGCAACCCCAGCGCTGGTTGCCTGGTTCATTCGGACCAGGGGTCTGTCTACACCAGCGATGACTGGCG
CAGTTTCCTGGCGTCCCATGGCTTGGTGTGCAGCATGAGTCGGCGTGGCAACTGCCACGAGCGCGAAGCCTGCCCTTGGG
GCACAACGCACCGGTGGAGAGCTTCTTTGGTCTGCTCAAACGCGAGCGGATCAGGCGGCGGATCTACCCCACCAAGGACG
CCGCACGCGCCGAGGTGTTCGACTACATCGAGATGTTCTACAACCCACAACGCCGTCATGGTTCAACTGGCGACCTGTCC
CCTGTAG

Upstream 100 bases:

>100_bases
AGCGCGACATCCTAAAAAAGGCCGCCGCGTACTTTGCCAAGGGGTAAAGGCAAAGTACCTCTTCATGCAAGTCCACCGCG
AAGAATTCAGGGTGTGCGCG

Downstream 100 bases:

>100_bases
AGTTTGAACGGCGCTACGCGCAACGAGGGTCTTGAGTGTCTACGGAACCCTGGGCGTATCAAACACCACGACTTGTTCGC
CGATTGCCGGCGCCGACCAG

Product: ISxac3 transposase

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MCRVLRVNRAGYYAWLKSPDSERAKEDERLLGLIKHHWLASGSVYGHRKIAKDLRDLGERCSRHRVHRLMRTEGLRAQVG
YGRKPRFHGGTPCKAAANLLDRQFDVTEPDTAWASDFTFIRTHEGWMYLAVVIDLFSRQVVGWAMRDRADTELVVQALLS
AVWRRNPSAGCLVHSDQGSVYTSDDWRSFLASHGLVCSMSRRGNCHEREACPWGTTHRWRASLVCSNASGSGGGSTPPRT
PHAPRCSTTSRCSTTHNAVMVQLATCPL

Sequences:

>Translated_268_residues
MCRVLRVNRAGYYAWLKSPDSERAKEDERLLGLIKHHWLASGSVYGHRKIAKDLRDLGERCSRHRVHRLMRTEGLRAQVG
YGRKPRFHGGTPCKAAANLLDRQFDVTEPDTAWASDFTFIRTHEGWMYLAVVIDLFSRQVVGWAMRDRADTELVVQALLS
AVWRRNPSAGCLVHSDQGSVYTSDDWRSFLASHGLVCSMSRRGNCHEREACPWGTTHRWRASLVCSNASGSGGGSTPPRT
PHAPRCSTTSRCSTTHNAVMVQLATCPL
>Mature_268_residues
MCRVLRVNRAGYYAWLKSPDSERAKEDERLLGLIKHHWLASGSVYGHRKIAKDLRDLGERCSRHRVHRLMRTEGLRAQVG
YGRKPRFHGGTPCKAAANLLDRQFDVTEPDTAWASDFTFIRTHEGWMYLAVVIDLFSRQVVGWAMRDRADTELVVQALLS
AVWRRNPSAGCLVHSDQGSVYTSDDWRSFLASHGLVCSMSRRGNCHEREACPWGTTHRWRASLVCSNASGSGGGSTPPRT
PHAPRCSTTSRCSTTHNAVMVQLATCPL

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=212, Percent_Identity=33.0188679245283, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1788405, Length=213, Percent_Identity=37.0892018779343, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1787263, Length=213, Percent_Identity=37.0892018779343, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1786753, Length=213, Percent_Identity=37.0892018779343, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1786570, Length=213, Percent_Identity=37.0892018779343, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1786490, Length=213, Percent_Identity=37.0892018779343, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1789981, Length=220, Percent_Identity=30.4545454545455, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 30075; Mature: 30075

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRVLRVNRAGYYAWLKSPDSERAKEDERLLGLIKHHWLASGSVYGHRKIAKDLRDLGER
CCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
CSRHRVHRLMRTEGLRAQVGYGRKPRFHGGTPCKAAANLLDRQFDVTEPDTAWASDFTFI
HHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHCCCEEE
RTHEGWMYLAVVIDLFSRQVVGWAMRDRADTELVVQALLSAVWRRNPSAGCLVHSDQGSV
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCE
YTSDDWRSFLASHGLVCSMSRRGNCHEREACPWGTTHRWRASLVCSNASGSGGGSTPPRT
EECHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCC
PHAPRCSTTSRCSTTHNAVMVQLATCPL
CCCCCCCCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MCRVLRVNRAGYYAWLKSPDSERAKEDERLLGLIKHHWLASGSVYGHRKIAKDLRDLGER
CCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
CSRHRVHRLMRTEGLRAQVGYGRKPRFHGGTPCKAAANLLDRQFDVTEPDTAWASDFTFI
HHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHCCCEEE
RTHEGWMYLAVVIDLFSRQVVGWAMRDRADTELVVQALLSAVWRRNPSAGCLVHSDQGSV
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCE
YTSDDWRSFLASHGLVCSMSRRGNCHEREACPWGTTHRWRASLVCSNASGSGGGSTPPRT
EECHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCC
PHAPRCSTTSRCSTTHNAVMVQLATCPL
CCCCCCCCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]