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The map label for this gene is fruB [H]

Identifier: 21243234

GI number: 21243234

Start: 2924373

End: 2926889

Strand: Direct

Name: fruB [H]

Synonym: XAC2501

Alternate gene names: 21243234

Gene position: 2924373-2926889 (Clockwise)

Preceding gene: 21243232

Following gene: 21243235

Centisome position: 56.5

GC content: 71.28

Gene sequence:

>2517_bases
TTGTCCTCTCCGTCGCCCGCCCCCGTCACCCCCGACCTTGTGCGCCTGCGCGCCTCAGCCCGCGACAAGGACGACGCCAT
CGCCCAGGCCGCCCAGTTACTGGTCGCGGCCGGCTGCGTCGCGCCGGGCTACGAGGCCAGCATGCGGCGGCGCGAAGGCC
TGGCGAACACCTTCCTGGGCCACGGCCTGGCGATCCCGCACGGCGTCGGCGAAGACCGCCACCTGGTGCGCCGCGACGGC
ATCGCAGTGCTGCAGCTCCCCGACGGCGTGGAATGGAACCCCGGCCAGATCACCCGGCTGGTGGTCGGCATCGCCGCGCA
ATCGGACACCCACATCACCTTGCTGCGCCGGCTGACCCGGTTGATCCAGGATCCGGCGCAGCTGGAAGCGCTGTTCAGTA
CCGACGACCCCAGCGTGATCGTCGCCGCACTCACCGGCGACCGCGCACCCGAGACCAGCGCCGCGCCGGCCACCGACCTG
GCCGAAGCATTCGAGTGGACCATCGCCTACCCCAGCGGCCTGCACGCGCGCCCGGCCACCCGCTGGGCCGAGACCGCACG
CGGCTTCTCGGCACGCGCGCAGGTACGCGCCGGCGACCAGGCCGCCGATGCCAAGAGCCTGGTCGGCTTGCTGCAGCTGG
GCCTGCGTGCCGGCGACAGCATCACCGTCTCGGCCGAAGGCAGCGACGCAGCGGAGCTGCTCAAGCGCCTGCGCGCCGTC
ATGGACAGCCTGACCGCGCAGGAAAAGGCCGATGCCGAGCGCGCCGCGCAACGTCGCGCCGCCCCGGTGGCCGGCTGGAC
GCCGCCGCAAGCGCAGCCGGCCATTGTCGGCATTGGTGCCAGCCCGGGCGTGGCGATCGGCATCGTCCATCGCCTGCGCG
CCGCGCAGACCGAAGTGGCCGATCAACCGGTGGGCCTGGGCGATGGCGGCGCGCAACTGCACGACGCGCTGACCCGCACC
CGCCAGCAGCTCGCGGCGATTCAGGACGACACCCAGCGCCGGCTCGGCGCCTCGGACGCGGCCATCTTCAAGGCCCAGGC
CGAACTGCTCAACGACACCGACCTGATCACCCGCACCTGCCAACTGATGGTCGAAGGCCATGGCGTGGCGTGGTCGTGGC
ACCAGGCGGTGGAACAGATCGCCTCCGGCCTGGCCGCACTCGGCAACCCGGTGCTGGCCGGTCGCGCGGCCGATCTGCGC
GATGTCGGCCGGCGCGTGCTGGCCCAGCTCGACCCGGCCGCGGCCGGCGCCGGTCTCACCGACCTGCCCGATCAGCCGTG
CATCCTGCTCGCCAGCGACCTGTCGCCGTCGGATACCGCCAATCTGGACACCGCGCGCGTGCTCGGCCTGGCCACCGCGC
AGGGCGGCCCAACCTCGCACACCGCCATCCTCTCGCGCACGCTCGGCCTGCCGGCGCTGGTCGCGGCCGGCGGCCAGTTG
CTGGAAATCGAAGACGGCGTCACCGCCATCATCGACGGCAGCAGCGGGCGTCTGTACATCGACCCGTCCGAGCAGGATCT
GGACGCAGCACGCACGCATATCGCCGAGCAGCAGGCCATTCGCGAACGCGAAGCGGCGCAGCGCGCGTTGCCGGCCGAAA
CCACCGACGGCCACCACATCGACATCGGCGCCAACGTCAATCTACCCGACCAGGTCGCGATGGCGCTGACCCAGGGCGCC
GAGGGCGTTGGCCTGATGCGCACCGAATTCCTGTTCCTGGAAAGCGGCCGCACCCCCAGCGAAGACGAACAGCACGCCAC
CTACCTGGCAATGGCGCAGGCGCTGGATGGCCGCCCGCTGATCGTGCGTGCGCTGGATATCGGCGGCGACAAGCAAGTGG
CGCATCTGGAATTGCCGCACGAAGAAAACCCGTTCCTGGGCGTGCGCGGCGCGCGCCTGCTGCTGCGTCGCCCCGACCTG
CTGGAGCCGCAACTGCGTGCGCTGTATCGCGCCGCCAAGGACGGCGCGCGGCTGTCGATCATGTTCCCGATGATCACCTC
GGTGCCGGAACTGGTCGCGCTGCGCGCCATCTGCGCACGCATCCGCGCCGACCTCGACGCACCGGAAGTGCCGATCGGCA
TCATGATCGAAGTGCCGGCCGCCGCCGCGCAGGCCGACGTGCTGGCGCGCCACGCAGACTTCTTCTCGATCGGCACCAAC
GACCTCACCCAGTACGTGCTGGCGATCGACCGCCAGAACCCGGAACTGGCGGCAGAAGCCGACAGCCTGCACCCGGCGGT
GCTGCGCATGATCCGCAGCACCATCGAAGGCGCACGCATGCACGACCGCTGGGTCGGCGTCTGCGGCGGCCTGGCCGGCG
ATGCGTTCGGCGCCAGCCTGCTGGCCGGCCTGGGCGTGCAGGAATTGTCGATGACGCCCAACGACATCCCGGCGGTCAAG
GCACGCCTGCGCGGCGCCGCGTTGAGCCAGCTGCAACAGCTCGCCGAACAAGCGCTGGCCTGCGAAACCGCCGAACAGGT
GCGCGCATTGGAAGCCAAGCGCGAGGACAAGGCATGA

Upstream 100 bases:

>100_bases
AACCCGAAAGCACTACAGCGTTTTTGTGCACCGCACATTGACCTGAATCGTGGGAACGTTCCCACTATACTTCCCAGCCC
CACCTCATCCGGAGCCCGCC

Downstream 100 bases:

>100_bases
GCCTGCAGGCCATCACCGTCACGCTGAACCCGGCGATCGACCAGACCATCCAGCTCGAACACCTGCAGCCTGGCGCCGTG
CACCGCGCCAGCAGCGTGCG

Product: multiphosphoryl transfer protein

Products: NA

Alternate protein names: MTP; Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system enzyme I; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; EIII-Fru; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 838; Mature: 837

Protein sequence:

>838_residues
MSSPSPAPVTPDLVRLRASARDKDDAIAQAAQLLVAAGCVAPGYEASMRRREGLANTFLGHGLAIPHGVGEDRHLVRRDG
IAVLQLPDGVEWNPGQITRLVVGIAAQSDTHITLLRRLTRLIQDPAQLEALFSTDDPSVIVAALTGDRAPETSAAPATDL
AEAFEWTIAYPSGLHARPATRWAETARGFSARAQVRAGDQAADAKSLVGLLQLGLRAGDSITVSAEGSDAAELLKRLRAV
MDSLTAQEKADAERAAQRRAAPVAGWTPPQAQPAIVGIGASPGVAIGIVHRLRAAQTEVADQPVGLGDGGAQLHDALTRT
RQQLAAIQDDTQRRLGASDAAIFKAQAELLNDTDLITRTCQLMVEGHGVAWSWHQAVEQIASGLAALGNPVLAGRAADLR
DVGRRVLAQLDPAAAGAGLTDLPDQPCILLASDLSPSDTANLDTARVLGLATAQGGPTSHTAILSRTLGLPALVAAGGQL
LEIEDGVTAIIDGSSGRLYIDPSEQDLDAARTHIAEQQAIREREAAQRALPAETTDGHHIDIGANVNLPDQVAMALTQGA
EGVGLMRTEFLFLESGRTPSEDEQHATYLAMAQALDGRPLIVRALDIGGDKQVAHLELPHEENPFLGVRGARLLLRRPDL
LEPQLRALYRAAKDGARLSIMFPMITSVPELVALRAICARIRADLDAPEVPIGIMIEVPAAAAQADVLARHADFFSIGTN
DLTQYVLAIDRQNPELAAEADSLHPAVLRMIRSTIEGARMHDRWVGVCGGLAGDAFGASLLAGLGVQELSMTPNDIPAVK
ARLRGAALSQLQQLAEQALACETAEQVRALEAKREDKA

Sequences:

>Translated_838_residues
MSSPSPAPVTPDLVRLRASARDKDDAIAQAAQLLVAAGCVAPGYEASMRRREGLANTFLGHGLAIPHGVGEDRHLVRRDG
IAVLQLPDGVEWNPGQITRLVVGIAAQSDTHITLLRRLTRLIQDPAQLEALFSTDDPSVIVAALTGDRAPETSAAPATDL
AEAFEWTIAYPSGLHARPATRWAETARGFSARAQVRAGDQAADAKSLVGLLQLGLRAGDSITVSAEGSDAAELLKRLRAV
MDSLTAQEKADAERAAQRRAAPVAGWTPPQAQPAIVGIGASPGVAIGIVHRLRAAQTEVADQPVGLGDGGAQLHDALTRT
RQQLAAIQDDTQRRLGASDAAIFKAQAELLNDTDLITRTCQLMVEGHGVAWSWHQAVEQIASGLAALGNPVLAGRAADLR
DVGRRVLAQLDPAAAGAGLTDLPDQPCILLASDLSPSDTANLDTARVLGLATAQGGPTSHTAILSRTLGLPALVAAGGQL
LEIEDGVTAIIDGSSGRLYIDPSEQDLDAARTHIAEQQAIREREAAQRALPAETTDGHHIDIGANVNLPDQVAMALTQGA
EGVGLMRTEFLFLESGRTPSEDEQHATYLAMAQALDGRPLIVRALDIGGDKQVAHLELPHEENPFLGVRGARLLLRRPDL
LEPQLRALYRAAKDGARLSIMFPMITSVPELVALRAICARIRADLDAPEVPIGIMIEVPAAAAQADVLARHADFFSIGTN
DLTQYVLAIDRQNPELAAEADSLHPAVLRMIRSTIEGARMHDRWVGVCGGLAGDAFGASLLAGLGVQELSMTPNDIPAVK
ARLRGAALSQLQQLAEQALACETAEQVRALEAKREDKA
>Mature_837_residues
SSPSPAPVTPDLVRLRASARDKDDAIAQAAQLLVAAGCVAPGYEASMRRREGLANTFLGHGLAIPHGVGEDRHLVRRDGI
AVLQLPDGVEWNPGQITRLVVGIAAQSDTHITLLRRLTRLIQDPAQLEALFSTDDPSVIVAALTGDRAPETSAAPATDLA
EAFEWTIAYPSGLHARPATRWAETARGFSARAQVRAGDQAADAKSLVGLLQLGLRAGDSITVSAEGSDAAELLKRLRAVM
DSLTAQEKADAERAAQRRAAPVAGWTPPQAQPAIVGIGASPGVAIGIVHRLRAAQTEVADQPVGLGDGGAQLHDALTRTR
QQLAAIQDDTQRRLGASDAAIFKAQAELLNDTDLITRTCQLMVEGHGVAWSWHQAVEQIASGLAALGNPVLAGRAADLRD
VGRRVLAQLDPAAAGAGLTDLPDQPCILLASDLSPSDTANLDTARVLGLATAQGGPTSHTAILSRTLGLPALVAAGGQLL
EIEDGVTAIIDGSSGRLYIDPSEQDLDAARTHIAEQQAIREREAAQRALPAETTDGHHIDIGANVNLPDQVAMALTQGAE
GVGLMRTEFLFLESGRTPSEDEQHATYLAMAQALDGRPLIVRALDIGGDKQVAHLELPHEENPFLGVRGARLLLRRPDLL
EPQLRALYRAAKDGARLSIMFPMITSVPELVALRAICARIRADLDAPEVPIGIMIEVPAAAAQADVLARHADFFSIGTND
LTQYVLAIDRQNPELAAEADSLHPAVLRMIRSTIEGARMHDRWVGVCGGLAGDAFGASLLAGLGVQELSMTPNDIPAVKA
RLRGAALSQLQQLAEQALACETAEQVRALEAKREDKA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=572, Percent_Identity=38.6363636363636, Blast_Score=361, Evalue=1e-100,
Organism=Escherichia coli, GI48994992, Length=704, Percent_Identity=32.1022727272727, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1788726, Length=680, Percent_Identity=31.9117647058824, Blast_Score=289, Evalue=4e-79,
Organism=Escherichia coli, GI1789193, Length=499, Percent_Identity=35.6713426853707, Blast_Score=275, Evalue=8e-75,
Organism=Escherichia coli, GI1788494, Length=133, Percent_Identity=44.3609022556391, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1787994, Length=417, Percent_Identity=25.1798561151079, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI226510935, Length=352, Percent_Identity=26.4204545454545, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1790027, Length=137, Percent_Identity=35.036496350365, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 88388; Mature: 88257

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00369 PTS_HPR_HIS ; PS00589 PTS_HPR_SER ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPSPAPVTPDLVRLRASARDKDDAIAQAAQLLVAAGCVAPGYEASMRRREGLANTFLG
CCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
HGLAIPHGVGEDRHLVRRDGIAVLQLPDGVEWNPGQITRLVVGIAAQSDTHITLLRRLTR
CCCCCCCCCCCCCHHHHHCCEEEEECCCCCCCCCCHHEEEEEEEECCCCCHHHHHHHHHH
LIQDPAQLEALFSTDDPSVIVAALTGDRAPETSAAPATDLAEAFEWTIAYPSGLHARPAT
HHCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHEEEEECCCCCCCCCHH
RWAETARGFSARAQVRAGDQAADAKSLVGLLQLGLRAGDSITVSAEGSDAAELLKRLRAV
HHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHH
MDSLTAQEKADAERAAQRRAAPVAGWTPPQAQPAIVGIGASPGVAIGIVHRLRAAQTEVA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
DQPVGLGDGGAQLHDALTRTRQQLAAIQDDTQRRLGASDAAIFKAQAELLNDTDLITRTC
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHH
QLMVEGHGVAWSWHQAVEQIASGLAALGNPVLAGRAADLRDVGRRVLAQLDPAAAGAGLT
HHHEECCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHCCCCCC
DLPDQPCILLASDLSPSDTANLDTARVLGLATAQGGPTSHTAILSRTLGLPALVAAGGQL
CCCCCCEEEEECCCCCCCCCCCCHHHHHEEEECCCCCCHHHHHHHHHCCCHHHHHCCCCE
LEIEDGVTAIIDGSSGRLYIDPSEQDLDAARTHIAEQQAIREREAAQRALPAETTDGHHI
EEECCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
DIGANVNLPDQVAMALTQGAEGVGLMRTEFLFLESGRTPSEDEQHATYLAMAQALDGRPL
EECCCCCCHHHHHHHHHCCCCCCCCHHEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCE
IVRALDIGGDKQVAHLELPHEENPFLGVRGARLLLRRPDLLEPQLRALYRAAKDGARLSI
EEEEEECCCCCEEEEEECCCCCCCEEECCHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEE
MFPMITSVPELVALRAICARIRADLDAPEVPIGIMIEVPAAAAQADVLARHADFFSIGTN
EHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEECCCH
DLTQYVLAIDRQNPELAAEADSLHPAVLRMIRSTIEGARMHDRWVGVCGGLAGDAFGASL
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHH
LAGLGVQELSMTPNDIPAVKARLRGAALSQLQQLAEQALACETAEQVRALEAKREDKA
HHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSPSPAPVTPDLVRLRASARDKDDAIAQAAQLLVAAGCVAPGYEASMRRREGLANTFLG
CCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
HGLAIPHGVGEDRHLVRRDGIAVLQLPDGVEWNPGQITRLVVGIAAQSDTHITLLRRLTR
CCCCCCCCCCCCCHHHHHCCEEEEECCCCCCCCCCHHEEEEEEEECCCCCHHHHHHHHHH
LIQDPAQLEALFSTDDPSVIVAALTGDRAPETSAAPATDLAEAFEWTIAYPSGLHARPAT
HHCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHEEEEECCCCCCCCCHH
RWAETARGFSARAQVRAGDQAADAKSLVGLLQLGLRAGDSITVSAEGSDAAELLKRLRAV
HHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHH
MDSLTAQEKADAERAAQRRAAPVAGWTPPQAQPAIVGIGASPGVAIGIVHRLRAAQTEVA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
DQPVGLGDGGAQLHDALTRTRQQLAAIQDDTQRRLGASDAAIFKAQAELLNDTDLITRTC
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHH
QLMVEGHGVAWSWHQAVEQIASGLAALGNPVLAGRAADLRDVGRRVLAQLDPAAAGAGLT
HHHEECCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHCCCCCC
DLPDQPCILLASDLSPSDTANLDTARVLGLATAQGGPTSHTAILSRTLGLPALVAAGGQL
CCCCCCEEEEECCCCCCCCCCCCHHHHHEEEECCCCCCHHHHHHHHHCCCHHHHHCCCCE
LEIEDGVTAIIDGSSGRLYIDPSEQDLDAARTHIAEQQAIREREAAQRALPAETTDGHHI
EEECCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
DIGANVNLPDQVAMALTQGAEGVGLMRTEFLFLESGRTPSEDEQHATYLAMAQALDGRPL
EECCCCCCHHHHHHHHHCCCCCCCCHHEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCE
IVRALDIGGDKQVAHLELPHEENPFLGVRGARLLLRRPDLLEPQLRALYRAAKDGARLSI
EEEEEECCCCCEEEEEECCCCCCCEEECCHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEE
MFPMITSVPELVALRAICARIRADLDAPEVPIGIMIEVPAAAAQADVLARHADFFSIGTN
EHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEECCCH
DLTQYVLAIDRQNPELAAEADSLHPAVLRMIRSTIEGARMHDRWVGVCGGLAGDAFGASL
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHH
LAGLGVQELSMTPNDIPAVKARLRGAALSQLQQLAEQALACETAEQVRALEAKREDKA
HHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7496537; 12024217; 1655739 [H]