Click here to switch to the map view.

The map label for this gene is argG

Identifier: 21243085

GI number: 21243085

Start: 2746102

End: 2747340

Strand: Reverse

Name: argG

Synonym: XAC2351

Alternate gene names: 21243085

Gene position: 2747340-2746102 (Counterclockwise)

Preceding gene: 21243086

Following gene: 77748648

Centisome position: 53.08

GC content: 64.0

Gene sequence:

>1239_bases
ATGATGAGCGCTGCTTCCACCAATCCCGAATTCCCCATCGCGACTTCCGGCACCAAGGACATCGTTCTCGCGTTTTCCGG
TGGTCTGGACACCAGCTTCTGCATTCCGTATCTGCAGGAGCGCGGCTACGCGGTGCACACCGTGTTTGCCGATACCGGTG
GTGTGGATGCCGAAGAACGTGAGTTCATCGAGAAGCGCGCTGCCGAATTGGGCGCGGCCAGCCACGTCACCGTGGACGGT
GGCCCGGCGATCTGGGAAGGCTTCGTCAAGCCGTTCGTGTGGGCGGGCGAGGGGTACCAGGGCCAGTATCCGTTGCTGGT
GTCCGACCGCTACCTGATCGTGGATGCTGCGCTCAAGCGTGCCGAAGAATTGGGCACGCGCATCATTGCGCACGGTTGCA
CCGGCATGGGCAACGACCAGGTGCGTTTCGACCTGGCAGTGAAGGCGCTGGGCGACTATCAGATCGTCGCGCCGATTCGC
GAGATCCAGAAAGAGCACACCCAGACCCGTGCCTATGAGCAGAAGTATCTGGAAGCGCGTGGCTTCGGCGTGCGTGCCAA
ACAGCAGGCCTACACCATCAATGAGAACCTGCTGGGCCTGACCATGTCCGGCGGCGAGATCGACCGTTGGGAAGCGCCGG
GCGAGGGCGCGCGCGGCTGGTGCGCGCCGCGCAGCGCCTGGCCGAGCGAAGCGCTGACGGTGACGCTGAAGTTCGTCGAA
GGCGAAGCGGTGGAACTGGACGGCAAGCCGCTGCCGGGTGCGAAGATCCTGGCCAAGCTCAACACCTTGTTCGCGCAGTA
CGGCGTGGGCCGCGGCGTCTATACCGGCGATACCGTGATCGGTTTGAAGGGCCGCATCGTATTCGAAGCGCCGGGCCTGA
TTTCGCTGCTCACCGCGCACCGTGCACTGGAAGATGCGGTGCTGACCAAGCAGCAGAACCGCTTCAAGCCGGACGTGGCG
CGCAAGTGGGTGGAGCTGGTGTACGAAGGCTTCTATCACGACCCGCTCAAGAGCGATATCGAGGCGTTCCTGAAGTCCTC
GCAGGCCAAGGTCAATGGCGAGGTGACGCTGGAAACCCGCGGGGGGCGCGTCGATGCGGTGGCGGTGCGTTCGCCGCACC
TGCTCAACACCAAGGGTGCGACCTACGCGCAATCGGCCGACTGGGGCGTGGAAGAGGCCGAGGGCTTCATCAAGCTGTTC
GGGATGAGCTCCACGCTGTACGCGCAGGTCAACCGCTGA

Upstream 100 bases:

>100_bases
GCGATCATGGCGGCGCTTGCTTCGCAAGCCGGAATCGGGAATCGGGAATAGAGAATCGCAACAGCAACACCTTGGCCCCC
ACACTACGTACTAAGGAACA

Downstream 100 bases:

>100_bases
GCAAAAGGCCCCTCTCTCTTCCATGGGGAGGGGAGAAGAGGGCCACTGCGATGGAACCCATGCCCGCACTACGCATCAGT
ACCGACAAGCAGGAACTCGA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MMSAASTNPEFPIATSGTKDIVLAFSGGLDTSFCIPYLQERGYAVHTVFADTGGVDAEEREFIEKRAAELGAASHVTVDG
GPAIWEGFVKPFVWAGEGYQGQYPLLVSDRYLIVDAALKRAEELGTRIIAHGCTGMGNDQVRFDLAVKALGDYQIVAPIR
EIQKEHTQTRAYEQKYLEARGFGVRAKQQAYTINENLLGLTMSGGEIDRWEAPGEGARGWCAPRSAWPSEALTVTLKFVE
GEAVELDGKPLPGAKILAKLNTLFAQYGVGRGVYTGDTVIGLKGRIVFEAPGLISLLTAHRALEDAVLTKQQNRFKPDVA
RKWVELVYEGFYHDPLKSDIEAFLKSSQAKVNGEVTLETRGGRVDAVAVRSPHLLNTKGATYAQSADWGVEEAEGFIKLF
GMSSTLYAQVNR

Sequences:

>Translated_412_residues
MMSAASTNPEFPIATSGTKDIVLAFSGGLDTSFCIPYLQERGYAVHTVFADTGGVDAEEREFIEKRAAELGAASHVTVDG
GPAIWEGFVKPFVWAGEGYQGQYPLLVSDRYLIVDAALKRAEELGTRIIAHGCTGMGNDQVRFDLAVKALGDYQIVAPIR
EIQKEHTQTRAYEQKYLEARGFGVRAKQQAYTINENLLGLTMSGGEIDRWEAPGEGARGWCAPRSAWPSEALTVTLKFVE
GEAVELDGKPLPGAKILAKLNTLFAQYGVGRGVYTGDTVIGLKGRIVFEAPGLISLLTAHRALEDAVLTKQQNRFKPDVA
RKWVELVYEGFYHDPLKSDIEAFLKSSQAKVNGEVTLETRGGRVDAVAVRSPHLLNTKGATYAQSADWGVEEAEGFIKLF
GMSSTLYAQVNR
>Mature_412_residues
MMSAASTNPEFPIATSGTKDIVLAFSGGLDTSFCIPYLQERGYAVHTVFADTGGVDAEEREFIEKRAAELGAASHVTVDG
GPAIWEGFVKPFVWAGEGYQGQYPLLVSDRYLIVDAALKRAEELGTRIIAHGCTGMGNDQVRFDLAVKALGDYQIVAPIR
EIQKEHTQTRAYEQKYLEARGFGVRAKQQAYTINENLLGLTMSGGEIDRWEAPGEGARGWCAPRSAWPSEALTVTLKFVE
GEAVELDGKPLPGAKILAKLNTLFAQYGVGRGVYTGDTVIGLKGRIVFEAPGLISLLTAHRALEDAVLTKQQNRFKPDVA
RKWVELVYEGFYHDPLKSDIEAFLKSSQAKVNGEVTLETRGGRVDAVAVRSPHLLNTKGATYAQSADWGVEEAEGFIKLF
GMSSTLYAQVNR

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=409, Percent_Identity=27.1393643031785, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI16950633, Length=409, Percent_Identity=27.1393643031785, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1789563, Length=369, Percent_Identity=26.8292682926829, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324514, Length=360, Percent_Identity=29.1666666666667, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21358151, Length=396, Percent_Identity=30.3030303030303, Blast_Score=146, Evalue=3e-35,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_XANAC (Q8PK26)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642667.1
- ProteinModelPortal:   Q8PK26
- SMR:   Q8PK26
- GeneID:   1156422
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC2351
- NMPDR:   fig|190486.1.peg.2311
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- ProtClustDB:   PRK04527
- BioCyc:   XAXO190486:XAC2351-MONOMER
- BRENDA:   6.3.4.5
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44901; Mature: 44901

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 50-50 BINDING 103-103 BINDING 108-108 BINDING 132-132 BINDING 134-134 BINDING 138-138 BINDING 138-138 BINDING 139-139 BINDING 142-142

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSAASTNPEFPIATSGTKDIVLAFSGGLDTSFCIPYLQERGYAVHTVFADTGGVDAEER
CCCCCCCCCCCCEECCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHH
EFIEKRAAELGAASHVTVDGGPAIWEGFVKPFVWAGEGYQGQYPLLVSDRYLIVDAALKR
HHHHHHHHHCCCCCEEEECCCHHHHHHHHCCEEECCCCCCCCCCEEECCCEEEEHHHHHH
AEELGTRIIAHGCTGMGNDQVRFDLAVKALGDYQIVAPIREIQKEHTQTRAYEQKYLEAR
HHHHHHEEEECCCCCCCCCCEEEEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHC
GFGVRAKQQAYTINENLLGLTMSGGEIDRWEAPGEGARGWCAPRSAWPSEALTVTLKFVE
CCCCCCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEC
GEAVELDGKPLPGAKILAKLNTLFAQYGVGRGVYTGDTVIGLKGRIVFEAPGLISLLTAH
CCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEECCCEEEEECCEEEEECCCHHHHHHHH
RALEDAVLTKQQNRFKPDVARKWVELVYEGFYHDPLKSDIEAFLKSSQAKVNGEVTLETR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEEEC
GGRVDAVAVRSPHLLNTKGATYAQSADWGVEEAEGFIKLFGMSSTLYAQVNR
CCEEEEEEECCCCEECCCCCCCCCCCCCCHHHHCCEEEEEECCCEEEEEECC
>Mature Secondary Structure
MMSAASTNPEFPIATSGTKDIVLAFSGGLDTSFCIPYLQERGYAVHTVFADTGGVDAEER
CCCCCCCCCCCCEECCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHH
EFIEKRAAELGAASHVTVDGGPAIWEGFVKPFVWAGEGYQGQYPLLVSDRYLIVDAALKR
HHHHHHHHHCCCCCEEEECCCHHHHHHHHCCEEECCCCCCCCCCEEECCCEEEEHHHHHH
AEELGTRIIAHGCTGMGNDQVRFDLAVKALGDYQIVAPIREIQKEHTQTRAYEQKYLEAR
HHHHHHEEEECCCCCCCCCCEEEEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHC
GFGVRAKQQAYTINENLLGLTMSGGEIDRWEAPGEGARGWCAPRSAWPSEALTVTLKFVE
CCCCCCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEC
GEAVELDGKPLPGAKILAKLNTLFAQYGVGRGVYTGDTVIGLKGRIVFEAPGLISLLTAH
CCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEECCCEEEEECCEEEEECCCHHHHHHHH
RALEDAVLTKQQNRFKPDVARKWVELVYEGFYHDPLKSDIEAFLKSSQAKVNGEVTLETR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEEEC
GGRVDAVAVRSPHLLNTKGATYAQSADWGVEEAEGFIKLFGMSSTLYAQVNR
CCEEEEEEECCCCEECCCCCCCCCCCCCCHHHHCCEEEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217