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The map label for this gene is rluB

Identifier: 21243053

GI number: 21243053

Start: 2711970

End: 2713622

Strand: Direct

Name: rluB

Synonym: XAC2318

Alternate gene names: 21243053

Gene position: 2711970-2713622 (Clockwise)

Preceding gene: 21243052

Following gene: 21243055

Centisome position: 52.4

GC content: 70.24

Gene sequence:

>1653_bases
ATGAGTGACACCCCCCGCAAGCTGTCGTTGAACAAGCTTTCCCTCAAGCGCGACAGCGCGCCCGAAGCGCCCAAGCTGGA
AGAGCGCCTGCACAAGGTGCTGGCCCAGGCCGGCCTGGGTTCGCGCCGCGCGCTGGAACAGCGCATCGCCGACGGCCTGA
TCAAGGTCAATGGCGCCGTTGCGCAGACCGGCATGTCGGTGCGCAGCGGCGACAAGATCGAGTTGGACGGCCGCAGCTTC
GTGGCCAGCGCGCTGACCGAGCCCTCGCGCGTGCTGATATACAACAAGCCCGAAGGCGAAGTGACCACGCGCGAAGATCC
CGAAGGCCGCCCGACCGTGTTCGAGTCGCTGCCGGTCCTGAAGGGCTCGCGCTGGATCGCCATTGGCCGCCTGGACATCA
ACACCACCGGCCTGCTGCTGCTCACCACCGACGGCGAGCTGGCCAACGCGATGATGCACCCCTCCTACGAGGTCGAGCGC
GAATATGTGGTGCGCGTGCGCGCACCGGAAGGCGAAGAAAAAGTCTCCGATGCCATCATCGAGCGCCTGTCGCGCGGTGT
GCTGCTGGAAGACGGCGGCGCCAAGTTCGACGAGATCGAACGCATCGGCGGCACCGATTCGCACGACTGGTTTCGTGTGG
TCGTCAAGGAAGGCCGCAACCGCGAAGTGCGCCGTCTGTGGGAATCGCAGGGTTGCCAGGTCAGCCGCCTCAAGCGCAGC
CGCTACGGCAAGATTTCGCTGCCGCGCGAATTGCTGCGTGGGCACTCGGTGGAAGTGGCGCAGGACAAGGTCGACGCCTT
GCGCGCCGAACTGAAACTGGAAGAAGGCGCGCCGTCGGCACTGACGCTGCAGCCGGTGATCGGCCAGCGCCGCGCCGCCA
AGTCCACCGTGCACGTCTCGCGCGATGGCCGCAGCAATGCCTACGTCAACGGCCAGACCTCCGGTGCCGATGAAGGCCGC
GAGCTGCGCCGTTTCGACAATCTGCGCGAAGATCGCGGCCGCGGCGGTCGCGGCAAGCCGGGCGGCTTCAAGGGTGGCCT
GACGGTCAGCGGCGAAGCGGCCGCTCGGCAATCGCAGCAGCGCCCGTTCAAGCAGCGCGGCCCGGCCAAGGGCGACCGCG
GCGCGTTGCCGGACGGCAATCCGGCCGCGTTCCGCAGCTGGTATGTGCCCGATGGCGTGAGCACCGGCCCAAGCGGTCAC
CGCAATGCCGGCCCGAGCGGCCCGGGCACCGGTCAGGCACGCCCGTATGCGAAGAAAGGACCAGGCGGCGCGCGTCCGGG
TACCGGCGGTCCAGTTGGCGCCCGCTCGGGTGGTCCGGGGCGCGGCGCTGGCGGCGGACAGGGGCAGTCGCAAGGCCAGG
GGCAGCGCAAGCACCCGTATGGGCACCCGGGCAACGCACCGAGCTTCCCTTCCGATCACGCCAACCCGGGTTTCAATCCG
TATGGTGCGGCACGCCCTGCTGGCCGCCCGAGCGGTGGGCGTCCGGGACCGGGTGGCAATCGCGGCCCGGCATCCGCCAA
CCGCGGCCCCGGCGGTCCAGGTGGTGGCGCCCGCGGTCCCGGCGGCCCAGGTGGCGCACCGCGTGGCCCGGGCGGCCGTC
CGCCGGGCGGCGGTAATCGTCGCCCTCCTGGCGGCGGCAATCGCGGTCGTTGA

Upstream 100 bases:

>100_bases
GAAGACGAAGACAACGCCGTCGCGACGACGACCGTGGCTGTTGACGAAGCCGATTCCGACCCAGAGGCCGACCCGCAACG
CGGCGGCCGGAGCCAGACAC

Downstream 100 bases:

>100_bases
GCGTCAACTGCTCCGCACAATAGAAAACGGCCCTGCAAAGGGCCGTTTTCTATGGCGGACGGCATCGGCCATCTACTTAA
GACCGGGAAGTTGCGGCGCA

Product: pseudouridylate synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MSDTPRKLSLNKLSLKRDSAPEAPKLEERLHKVLAQAGLGSRRALEQRIADGLIKVNGAVAQTGMSVRSGDKIELDGRSF
VASALTEPSRVLIYNKPEGEVTTREDPEGRPTVFESLPVLKGSRWIAIGRLDINTTGLLLLTTDGELANAMMHPSYEVER
EYVVRVRAPEGEEKVSDAIIERLSRGVLLEDGGAKFDEIERIGGTDSHDWFRVVVKEGRNREVRRLWESQGCQVSRLKRS
RYGKISLPRELLRGHSVEVAQDKVDALRAELKLEEGAPSALTLQPVIGQRRAAKSTVHVSRDGRSNAYVNGQTSGADEGR
ELRRFDNLREDRGRGGRGKPGGFKGGLTVSGEAAARQSQQRPFKQRGPAKGDRGALPDGNPAAFRSWYVPDGVSTGPSGH
RNAGPSGPGTGQARPYAKKGPGGARPGTGGPVGARSGGPGRGAGGGQGQSQGQGQRKHPYGHPGNAPSFPSDHANPGFNP
YGAARPAGRPSGGRPGPGGNRGPASANRGPGGPGGGARGPGGPGGAPRGPGGRPPGGGNRRPPGGGNRGR

Sequences:

>Translated_550_residues
MSDTPRKLSLNKLSLKRDSAPEAPKLEERLHKVLAQAGLGSRRALEQRIADGLIKVNGAVAQTGMSVRSGDKIELDGRSF
VASALTEPSRVLIYNKPEGEVTTREDPEGRPTVFESLPVLKGSRWIAIGRLDINTTGLLLLTTDGELANAMMHPSYEVER
EYVVRVRAPEGEEKVSDAIIERLSRGVLLEDGGAKFDEIERIGGTDSHDWFRVVVKEGRNREVRRLWESQGCQVSRLKRS
RYGKISLPRELLRGHSVEVAQDKVDALRAELKLEEGAPSALTLQPVIGQRRAAKSTVHVSRDGRSNAYVNGQTSGADEGR
ELRRFDNLREDRGRGGRGKPGGFKGGLTVSGEAAARQSQQRPFKQRGPAKGDRGALPDGNPAAFRSWYVPDGVSTGPSGH
RNAGPSGPGTGQARPYAKKGPGGARPGTGGPVGARSGGPGRGAGGGQGQSQGQGQRKHPYGHPGNAPSFPSDHANPGFNP
YGAARPAGRPSGGRPGPGGNRGPASANRGPGGPGGGARGPGGPGGAPRGPGGRPPGGGNRRPPGGGNRGR
>Mature_549_residues
SDTPRKLSLNKLSLKRDSAPEAPKLEERLHKVLAQAGLGSRRALEQRIADGLIKVNGAVAQTGMSVRSGDKIELDGRSFV
ASALTEPSRVLIYNKPEGEVTTREDPEGRPTVFESLPVLKGSRWIAIGRLDINTTGLLLLTTDGELANAMMHPSYEVERE
YVVRVRAPEGEEKVSDAIIERLSRGVLLEDGGAKFDEIERIGGTDSHDWFRVVVKEGRNREVRRLWESQGCQVSRLKRSR
YGKISLPRELLRGHSVEVAQDKVDALRAELKLEEGAPSALTLQPVIGQRRAAKSTVHVSRDGRSNAYVNGQTSGADEGRE
LRRFDNLREDRGRGGRGKPGGFKGGLTVSGEAAARQSQQRPFKQRGPAKGDRGALPDGNPAAFRSWYVPDGVSTGPSGHR
NAGPSGPGTGQARPYAKKGPGGARPGTGGPVGARSGGPGRGAGGGQGQSQGQGQRKHPYGHPGNAPSFPSDHANPGFNPY
GAARPAGRPSGGRPGPGGNRGPASANRGPGGPGGGARGPGGPGGAPRGPGGRPPGGGNRRPPGGGNRGR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787524, Length=251, Percent_Identity=54.1832669322709, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI1790453, Length=224, Percent_Identity=33.4821428571429, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1788510, Length=227, Percent_Identity=32.15859030837, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87081838, Length=176, Percent_Identity=32.3863636363636, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLUB_XANAC (Q8PK58)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642635.1
- ProteinModelPortal:   Q8PK58
- SMR:   Q8PK58
- GeneID:   1156389
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC2318
- NMPDR:   fig|190486.1.peg.2279
- HOGENOM:   HBG754478
- OMA:   WGETDAR
- ProtClustDB:   CLSK446516
- BioCyc:   XAXO190486:XAC2318-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942
- SMART:   SM00363
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 57730; Mature: 57599

Theoretical pI: Translated: 11.22; Mature: 11.22

Prosite motif: PS01149 PSI_RSU; PS50889 S4

Important sites: ACT_SITE 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTPRKLSLNKLSLKRDSAPEAPKLEERLHKVLAQAGLGSRRALEQRIADGLIKVNGAV
CCCCCCEEECHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCEEEECCHH
AQTGMSVRSGDKIELDGRSFVASALTEPSRVLIYNKPEGEVTTREDPEGRPTVFESLPVL
HHCCCCCCCCCEEEECCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCCC
KGSRWIAIGRLDINTTGLLLLTTDGELANAMMHPSYEVEREYVVRVRAPEGEEKVSDAII
CCCCEEEEEEEEECCCEEEEEECCCHHHHHHHCCCHHCCCEEEEEEECCCCHHHHHHHHH
ERLSRGVLLEDGGAKFDEIERIGGTDSHDWFRVVVKEGRNREVRRLWESQGCQVSRLKRS
HHHHCCEEEECCCCCHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHH
RYGKISLPRELLRGHSVEVAQDKVDALRAELKLEEGAPSALTLQPVIGQRRAAKSTVHVS
CCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCHHHHCCEEEEE
RDGRSNAYVNGQTSGADEGRELRRFDNLREDRGRGGRGKPGGFKGGLTVSGEAAARQSQQ
CCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHH
RPFKQRGPAKGDRGALPDGNPAAFRSWYVPDGVSTGPSGHRNAGPSGPGTGQARPYAKKG
CCHHHCCCCCCCCCCCCCCCHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PGGARPGTGGPVGARSGGPGRGAGGGQGQSQGQGQRKHPYGHPGNAPSFPSDHANPGFNP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YGAARPAGRPSGGRPGPGGNRGPASANRGPGGPGGGARGPGGPGGAPRGPGGRPPGGGNR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RPPGGGNRGR
CCCCCCCCCC
>Mature Secondary Structure 
SDTPRKLSLNKLSLKRDSAPEAPKLEERLHKVLAQAGLGSRRALEQRIADGLIKVNGAV
CCCCCEEECHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCEEEECCHH
AQTGMSVRSGDKIELDGRSFVASALTEPSRVLIYNKPEGEVTTREDPEGRPTVFESLPVL
HHCCCCCCCCCEEEECCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCCC
KGSRWIAIGRLDINTTGLLLLTTDGELANAMMHPSYEVEREYVVRVRAPEGEEKVSDAII
CCCCEEEEEEEEECCCEEEEEECCCHHHHHHHCCCHHCCCEEEEEEECCCCHHHHHHHHH
ERLSRGVLLEDGGAKFDEIERIGGTDSHDWFRVVVKEGRNREVRRLWESQGCQVSRLKRS
HHHHCCEEEECCCCCHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHH
RYGKISLPRELLRGHSVEVAQDKVDALRAELKLEEGAPSALTLQPVIGQRRAAKSTVHVS
CCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCHHHHCCEEEEE
RDGRSNAYVNGQTSGADEGRELRRFDNLREDRGRGGRGKPGGFKGGLTVSGEAAARQSQQ
CCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHH
RPFKQRGPAKGDRGALPDGNPAAFRSWYVPDGVSTGPSGHRNAGPSGPGTGQARPYAKKG
CCHHHCCCCCCCCCCCCCCCHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PGGARPGTGGPVGARSGGPGRGAGGGQGQSQGQGQRKHPYGHPGNAPSFPSDHANPGFNP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YGAARPAGRPSGGRPGPGGNRGPASANRGPGGPGGGARGPGGPGGAPRGPGGRPPGGGNR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RPPGGGNRGR
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217