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The map label for this gene is purR [H]

Identifier: 21243048

GI number: 21243048

Start: 2707521

End: 2708543

Strand: Reverse

Name: purR [H]

Synonym: XAC2313

Alternate gene names: 21243048

Gene position: 2708543-2707521 (Counterclockwise)

Preceding gene: 21243049

Following gene: 21243047

Centisome position: 52.33

GC content: 69.21

Gene sequence:

>1023_bases
ATGGCCAAGGGTGCGGTCACCATCAAAGATGTCGCTCGGGAAGCGCAGGTCTCCGTCGCAACGGTGTCGCGTGCGCTCAA
CGGGCACGACAATGTTGCCGGGCCGGTGCGCCAGCAAGTGCTGGAAGTGGCCGAGCGGCTGCGCTACAGCCCGCATGCGG
CCGCGCGTAGCCTGAGCAGCCGGCGCTCGCAGACGCTGGGCGTGGTGCTGCCGGACTTGTATGGCGAATTCTTCTCCGAA
TTGATTCGCGGCATCGACGGCGCGGCGCGTGTGGCGGGCCAGCACCTGCTGGTGTCCAGCTATCACGGCGACCCGGAAGC
CCAGGGCAGGGCGCTGCGCTCGATGCGCGGACGCGTCGATGGTTTGCTGGTGCTCTCGCCCTACGCCGAGCAGCCGGGGT
TCCTCACCGATAACCTGCCGCAGTCGCTGCCAACGGTGTTGATCAATGCGCACCTGCCCGGTGCCGACTATCCGGTGCTG
AGCATCGACGACCACGCCGGTGCCATGGCCATGACCGAGCACCTGCTGCGTGCCGGGCACCGTCGCATCGCCTTCATCGG
TGGCCCGGCGCTGAATTTCGACGCGCGCGAACGCCTGCGCGGCTTCCGCGATGCGCTTGCTGCGTTCGGTGCGCAGGCGC
AGGGCGTGGAGTATCCGGGCGACTTCGACGAAGCCTCCGGCCACCGCGCCGGGCTGGCGATGCTGGCGGCCGGCGCGTTG
CCGGATGCGGTCTTCGCCGCCAACGACATGACTGCGCTGGGCTGCCTGTACGCGTTCGCCCAGGCCGATGTGTCGGTGCC
GGGCGACGTGGCGCTGGCGGGTTTCGACGACATTCCGCTGGCCCGTTTTGTCCACCCCGCGCTGACCACCATGCGCGTGA
GCATCGCCCGGCTGGGCGAGCAGGCCATGACCCGACTGATGCGCCTGGTCGAAACGCCCGACCGCGACGACGGCATCCGC
CAATTGCTCACGCCCGAGCTGGTGATCCGCGCCTCCTGCGGTGCCGGCGAACCGGCTCACTGA

Upstream 100 bases:

>100_bases
AAGCGTTTTCACGCGTGTGAAGATAGGGGTTTACGCCTCTGTATGAAAACGCTTACAGTCCGCGCCAGTTTTGGGAAGTC
GGTCGCGGAGGGCGACACGA

Downstream 100 bases:

>100_bases
CCTTTCCGGCCACGCGTCTCTGGGCCGCCGACCCTTTCGCGGGCGTTCTGTCCGCTCCCGCTGCCCCCGCAGCGGCTGTT
CCTGCACCACTGCACTGCCA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MAKGAVTIKDVAREAQVSVATVSRALNGHDNVAGPVRQQVLEVAERLRYSPHAAARSLSSRRSQTLGVVLPDLYGEFFSE
LIRGIDGAARVAGQHLLVSSYHGDPEAQGRALRSMRGRVDGLLVLSPYAEQPGFLTDNLPQSLPTVLINAHLPGADYPVL
SIDDHAGAMAMTEHLLRAGHRRIAFIGGPALNFDARERLRGFRDALAAFGAQAQGVEYPGDFDEASGHRAGLAMLAAGAL
PDAVFAANDMTALGCLYAFAQADVSVPGDVALAGFDDIPLARFVHPALTTMRVSIARLGEQAMTRLMRLVETPDRDDGIR
QLLTPELVIRASCGAGEPAH

Sequences:

>Translated_340_residues
MAKGAVTIKDVAREAQVSVATVSRALNGHDNVAGPVRQQVLEVAERLRYSPHAAARSLSSRRSQTLGVVLPDLYGEFFSE
LIRGIDGAARVAGQHLLVSSYHGDPEAQGRALRSMRGRVDGLLVLSPYAEQPGFLTDNLPQSLPTVLINAHLPGADYPVL
SIDDHAGAMAMTEHLLRAGHRRIAFIGGPALNFDARERLRGFRDALAAFGAQAQGVEYPGDFDEASGHRAGLAMLAAGAL
PDAVFAANDMTALGCLYAFAQADVSVPGDVALAGFDDIPLARFVHPALTTMRVSIARLGEQAMTRLMRLVETPDRDDGIR
QLLTPELVIRASCGAGEPAH
>Mature_339_residues
AKGAVTIKDVAREAQVSVATVSRALNGHDNVAGPVRQQVLEVAERLRYSPHAAARSLSSRRSQTLGVVLPDLYGEFFSEL
IRGIDGAARVAGQHLLVSSYHGDPEAQGRALRSMRGRVDGLLVLSPYAEQPGFLTDNLPQSLPTVLINAHLPGADYPVLS
IDDHAGAMAMTEHLLRAGHRRIAFIGGPALNFDARERLRGFRDALAAFGAQAQGVEYPGDFDEASGHRAGLAMLAAGALP
DAVFAANDMTALGCLYAFAQADVSVPGDVALAGFDDIPLARFVHPALTTMRVSIARLGEQAMTRLMRLVETPDRDDGIRQ
LLTPELVIRASCGAGEPAH

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=328, Percent_Identity=35.6707317073171, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI1789202, Length=299, Percent_Identity=35.4515050167224, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1790369, Length=337, Percent_Identity=33.5311572700297, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1788474, Length=306, Percent_Identity=33.3333333333333, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=33.6309523809524, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789068, Length=319, Percent_Identity=30.4075235109718, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1786540, Length=310, Percent_Identity=29.6774193548387, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787580, Length=314, Percent_Identity=28.343949044586, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1789456, Length=319, Percent_Identity=29.4670846394984, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI48994940, Length=315, Percent_Identity=28.5714285714286, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787906, Length=307, Percent_Identity=26.7100977198697, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=23.1511254019293, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1786268, Length=285, Percent_Identity=22.8070175438596, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790689, Length=307, Percent_Identity=22.8013029315961, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36153; Mature: 36021

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGAVTIKDVAREAQVSVATVSRALNGHDNVAGPVRQQVLEVAERLRYSPHAAARSLSS
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
RRSQTLGVVLPDLYGEFFSELIRGIDGAARVAGQHLLVSSYHGDPEAQGRALRSMRGRVD
HHHHHHHEECHHHHHHHHHHHHHCCCHHHHHHCCHHEEECCCCCCHHHHHHHHHHHCCCC
GLLVLSPYAEQPGFLTDNLPQSLPTVLINAHLPGADYPVLSIDDHAGAMAMTEHLLRAGH
EEEEECCCCCCCCCCCCCCHHHHHHEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHCCC
RRIAFIGGPALNFDARERLRGFRDALAAFGAQAQGVEYPGDFDEASGHRAGLAMLAAGAL
CEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
PDAVFAANDMTALGCLYAFAQADVSVPGDVALAGFDDIPLARFVHPALTTMRVSIARLGE
CHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
QAMTRLMRLVETPDRDDGIRQLLTPELVIRASCGAGEPAH
HHHHHHHHHHCCCCCCHHHHHHHCHHHEEEECCCCCCCCC
>Mature Secondary Structure 
AKGAVTIKDVAREAQVSVATVSRALNGHDNVAGPVRQQVLEVAERLRYSPHAAARSLSS
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
RRSQTLGVVLPDLYGEFFSELIRGIDGAARVAGQHLLVSSYHGDPEAQGRALRSMRGRVD
HHHHHHHEECHHHHHHHHHHHHHCCCHHHHHHCCHHEEECCCCCCHHHHHHHHHHHCCCC
GLLVLSPYAEQPGFLTDNLPQSLPTVLINAHLPGADYPVLSIDDHAGAMAMTEHLLRAGH
EEEEECCCCCCCCCCCCCCHHHHHHEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHCCC
RRIAFIGGPALNFDARERLRGFRDALAAFGAQAQGVEYPGDFDEASGHRAGLAMLAAGAL
CEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
PDAVFAANDMTALGCLYAFAQADVSVPGDVALAGFDDIPLARFVHPALTTMRVSIARLGE
CHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
QAMTRLMRLVETPDRDDGIRQLLTPELVIRASCGAGEPAH
HHHHHHHHHHCCCCCCHHHHHHHCHHHEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA