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The map label for this gene is intS [H]

Identifier: 21243021

GI number: 21243021

Start: 2673934

End: 2675160

Strand: Reverse

Name: intS [H]

Synonym: XAC2286

Alternate gene names: 21243021

Gene position: 2675160-2673934 (Counterclockwise)

Preceding gene: 21243022

Following gene: 21243003

Centisome position: 51.69

GC content: 59.33

Gene sequence:

>1227_bases
ATGAGCCGAATGAGGGCGAAGAACATGCTTACCGATACCAAGCTGCGTAACCTCAAGCCGAGGGATAGGCTCTACAAGGA
GAACGACCGTGATGGCCTCTATGTGGCCGTCACGCCTGCAGGTGCGATCTCGTTTCGCTATAACTATTCGATCCATGGCC
GGCAGGAGACCATCACTTTCGGCCGCTACGGTGTTGGCGGAATTACCCTTGCGGAAGCCCGAGAGCGGCTCGGGGAAGCC
AAGAAGATGATCGCGGCCGGGAAGTCGCCGGCCAAAGAGAAGGCGCGAGACAAGGCACGCGTTAAGGATGCGGAGACCTT
TGGGGCATGGTCGGAGAAGTGGCTGCGCGGCTACCAGATGGCTGACTCCACACGAGACATGCGCCGCTCGGTCTACGAGC
GCGAGTTAAAGCCGAAATTCGGCAATCAGAAGCTGGTGGAGATCACCCATGAGGACTTGCGCGCGCTCACCGATGCCATC
GTGGAGCGCGGCGCGCCGGCCACCGCAGTACATGCACGTGAGGTGATGTCGCAGGTCTATCGCTGGGCCATCGAACGCGG
CCAGAAGGTCGCCAACCCGGCAGAACTGGTGCGCCCAACGACCATCGCCAAGTTCGAGCCGCGCGACCGTGCGCTGACGC
CCGATGAGATCGGCCTGATGTATCAGTACATGGAGCGCATCGGTACGACGCCATCCATCCGGGCAGCAGCCAAATTGCTG
CTTCTGACGATGGTGCGCAAAAGCGAGCTGACCAACGCGACCTGGAGCGAGATCAATTTCAGCGACGCGCTTTGGACGAT
CCCGAAGGAGCGAATGAAGCGACGCAATCCGCACTTGGTATTTCTGTCTCGCCAAGCGCTGGACATTTTCATTGCCCTCA
AAACCTTTGCCGGCGGGTCGGACTACGTGTTGCCGTCGCGGTATGACTCAGACCTACCCATGAGCAGCGCCACGCTCAAC
CAAGTATTGACGCTCACATATCGGCTGGCGCAAAAGGAAGGAAAGTCGCTTCCCAAGTTTGGGCCACATGACCTACGGCG
CACGGCCAGCACGCTATTGCACGAGGCTGGCTACAACACCGACTGGATTGAGAAGTGCCTGGCGCATGAGCAGAAAGGCG
TCAGGGCCGTCTACAACAAGGCTGAGTACAGAGAGCAACGCACCGCTATGCTGCAGGACTGGGCGAACATGATCGACGAA
TGGACAATGAAGCGGCCGAGTGCTTGA

Upstream 100 bases:

>100_bases
CATGTGGCAATCTTCATGGCTATCATGGTACTAGATCTTCATAAGTATTTGATTTCATTTATTTTTTGCGCAAAATTTGG
CTGAATCTGGATCATGGTAT

Downstream 100 bases:

>100_bases
ACAAAGAGAGCACTTTGGGACGCTATCCGTACATCGATGCGTGACTTCGTTCTATTGTCGCCGGAGCAGCGCAGCGCTTT
TATCCGTTAAAGGGGCGGAG

Product: phage-related integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MSRMRAKNMLTDTKLRNLKPRDRLYKENDRDGLYVAVTPAGAISFRYNYSIHGRQETITFGRYGVGGITLAEARERLGEA
KKMIAAGKSPAKEKARDKARVKDAETFGAWSEKWLRGYQMADSTRDMRRSVYERELKPKFGNQKLVEITHEDLRALTDAI
VERGAPATAVHAREVMSQVYRWAIERGQKVANPAELVRPTTIAKFEPRDRALTPDEIGLMYQYMERIGTTPSIRAAAKLL
LLTMVRKSELTNATWSEINFSDALWTIPKERMKRRNPHLVFLSRQALDIFIALKTFAGGSDYVLPSRYDSDLPMSSATLN
QVLTLTYRLAQKEGKSLPKFGPHDLRRTASTLLHEAGYNTDWIEKCLAHEQKGVRAVYNKAEYREQRTAMLQDWANMIDE
WTMKRPSA

Sequences:

>Translated_408_residues
MSRMRAKNMLTDTKLRNLKPRDRLYKENDRDGLYVAVTPAGAISFRYNYSIHGRQETITFGRYGVGGITLAEARERLGEA
KKMIAAGKSPAKEKARDKARVKDAETFGAWSEKWLRGYQMADSTRDMRRSVYERELKPKFGNQKLVEITHEDLRALTDAI
VERGAPATAVHAREVMSQVYRWAIERGQKVANPAELVRPTTIAKFEPRDRALTPDEIGLMYQYMERIGTTPSIRAAAKLL
LLTMVRKSELTNATWSEINFSDALWTIPKERMKRRNPHLVFLSRQALDIFIALKTFAGGSDYVLPSRYDSDLPMSSATLN
QVLTLTYRLAQKEGKSLPKFGPHDLRRTASTLLHEAGYNTDWIEKCLAHEQKGVRAVYNKAEYREQRTAMLQDWANMIDE
WTMKRPSA
>Mature_407_residues
SRMRAKNMLTDTKLRNLKPRDRLYKENDRDGLYVAVTPAGAISFRYNYSIHGRQETITFGRYGVGGITLAEARERLGEAK
KMIAAGKSPAKEKARDKARVKDAETFGAWSEKWLRGYQMADSTRDMRRSVYERELKPKFGNQKLVEITHEDLRALTDAIV
ERGAPATAVHAREVMSQVYRWAIERGQKVANPAELVRPTTIAKFEPRDRALTPDEIGLMYQYMERIGTTPSIRAAAKLLL
LTMVRKSELTNATWSEINFSDALWTIPKERMKRRNPHLVFLSRQALDIFIALKTFAGGSDYVLPSRYDSDLPMSSATLNQ
VLTLTYRLAQKEGKSLPKFGPHDLRRTASTLLHEAGYNTDWIEKCLAHEQKGVRAVYNKAEYREQRTAMLQDWANMIDEW
TMKRPSA

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI145693166, Length=403, Percent_Identity=35.4838709677419, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1788974, Length=403, Percent_Identity=31.7617866004963, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1788690, Length=399, Percent_Identity=27.3182957393484, Blast_Score=137, Evalue=9e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 46727; Mature: 46596

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRMRAKNMLTDTKLRNLKPRDRLYKENDRDGLYVAVTPAGAISFRYNYSIHGRQETITF
CCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEEECCCEEEEEEEEEECCCCEEEEE
GRYGVGGITLAEARERLGEAKKMIAAGKSPAKEKARDKARVKDAETFGAWSEKWLRGYQM
CCCCCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADSTRDMRRSVYERELKPKFGNQKLVEITHEDLRALTDAIVERGAPATAVHAREVMSQVY
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RWAIERGQKVANPAELVRPTTIAKFEPRDRALTPDEIGLMYQYMERIGTTPSIRAAAKLL
HHHHHHCCCCCCHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
LLTMVRKSELTNATWSEINFSDALWTIPKERMKRRNPHLVFLSRQALDIFIALKTFAGGS
HHHHHHHHHHCCCCHHHCCCCHHHHCCCHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCC
DYVLPSRYDSDLPMSSATLNQVLTLTYRLAQKEGKSLPKFGPHDLRRTASTLLHEAGYNT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCH
DWIEKCLAHEQKGVRAVYNKAEYREQRTAMLQDWANMIDEWTMKRPSA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SRMRAKNMLTDTKLRNLKPRDRLYKENDRDGLYVAVTPAGAISFRYNYSIHGRQETITF
CCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEEECCCEEEEEEEEEECCCCEEEEE
GRYGVGGITLAEARERLGEAKKMIAAGKSPAKEKARDKARVKDAETFGAWSEKWLRGYQM
CCCCCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADSTRDMRRSVYERELKPKFGNQKLVEITHEDLRALTDAIVERGAPATAVHAREVMSQVY
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RWAIERGQKVANPAELVRPTTIAKFEPRDRALTPDEIGLMYQYMERIGTTPSIRAAAKLL
HHHHHHCCCCCCHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
LLTMVRKSELTNATWSEINFSDALWTIPKERMKRRNPHLVFLSRQALDIFIALKTFAGGS
HHHHHHHHHHCCCCHHHCCCCHHHHCCCHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCC
DYVLPSRYDSDLPMSSATLNQVLTLTYRLAQKEGKSLPKFGPHDLRRTASTLLHEAGYNT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCH
DWIEKCLAHEQKGVRAVYNKAEYREQRTAMLQDWANMIDEWTMKRPSA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]