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The map label for this gene is phaE

Identifier: 21242791

GI number: 21242791

Start: 2391564

End: 2392787

Strand: Direct

Name: phaE

Synonym: XAC2047

Alternate gene names: NA

Gene position: 2391564-2392787 (Clockwise)

Preceding gene: 21242790

Following gene: 21242792

Centisome position: 46.21

GC content: 67.48

Gene sequence:

>1224_bases
ATGGCAAGCAGCGGTTCGGACAACGGCAACTTCGACGACAAGGCGCGCCAATACTGGGCTGCCTGGGGAGATGCCATGCG
CCACGGCCAGGCCGGCGCCGCTGCGCAACAACCACCGCCCACGTCCGGTAGCGACGCCCCGCAGGATTGGCGCAAGGCCG
TGGATTGGTGGTCGCAATTGCTGCCCACCCAGGCCGCCCCGCAGGCGCAGGAGGCCATCGACCGCTTTCGCACCCAGGCC
GGCGACTGGTTTGGCACCATGCAGCAGGTGGCCGCGCAGTTTGCCGGGCGCGACACCTCGGCCAATGAAGTCGCCGATGC
CTGGCGCCAGGCCGTACAAGGGCAGGGTGAGCAGCTGATGCAGTGGACGCTGGGTTCGCTGCGCGGCGGCAGCCCGGGCG
GGTTCGACCCGTGGCTGCAGGACGCGGCGCAAGCGCTGCAAAAGTGGCGCGAGGAAAATGCGCCTTGGCTGGACATGCCG
GCGTTCGGGTTGAACCGCAACCACCAGTCGCGCCTGCAGAAACTGGCACGCGCGCAGCAGGAGTTCCAGGCCCAGTCGGA
GGCCTATGGCGAGCAACTCAAGGCGGCGATCGAGCAGGCGTTTGCGCGCTTCGCGTCCAGGCTGAGCGAGCACGAGAGCA
GCGGCAGCCAGTTGACCAGCGCCCGCGCCCTGTTCGATCTATGGATCGAAGCGGCCGAAGAGTCCTATGCCGATGTTGCC
TTGTCGGAGCAGTTCCGCAAGGTGTACGGCGGCTTTGCCAACGCGCACATGCGCCTGCGCGCTGCGCTGCAGGAAGAGGT
CGAGCAACTCAGCGAACGCTTCGGCATGCCCACACGTTCGGAGATGGACGCCGCGCATCGCCGCATTGCCGAACTGGAAC
GCCTGGTCAGGCGGATGCTGCGTAACGCAGCTTCGCCGGCGAGCAAGCCCGCTGCAGCGGCGCAGCCTGCACCGGTGACA
CCGGCAGCGGGCAAGCGCGCGTCGTCCGCCGGGGCCAGCGCTGTTGCGCGGCCGGCCGGGGCAGAACGGGCAGTCAAGCA
GGGTGCATCGAAGAAGGCACCCGTCGACAAGACCGCGACGAAAAAGGCGGCTCCGAAAAAGGCGCCAGCCGTTTCTAAGC
CGACGAAAACGACCTCGCCCAAGAAGCATGGTGGCGCGACATCCTCGCCCGAGCTTGCGGCCCGCAAACCCGTCGCAGCT
AAAAAACGTGGAGCGCAGGCATGA

Upstream 100 bases:

>100_bases
CTCGCTGATGATCAGCAAGACGCTGCGTATCCCCAAACCCTGAGCGGCACACCGCTACGCACGCGGTAGGATGGCTGGGT
AGAGGTCACTGGATGGGATG

Downstream 100 bases:

>100_bases
AAGGTCCGTTGGGATTCAGCGCCGAAGACCTGATGCAGGAAACCCTGTCGATGCAACGCAAGTTGCGCGAAGGACTCAAG
CTGCTGCCGGGCGTGGACGA

Product: PHA synthase subunit

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MASSGSDNGNFDDKARQYWAAWGDAMRHGQAGAAAQQPPPTSGSDAPQDWRKAVDWWSQLLPTQAAPQAQEAIDRFRTQA
GDWFGTMQQVAAQFAGRDTSANEVADAWRQAVQGQGEQLMQWTLGSLRGGSPGGFDPWLQDAAQALQKWREENAPWLDMP
AFGLNRNHQSRLQKLARAQQEFQAQSEAYGEQLKAAIEQAFARFASRLSEHESSGSQLTSARALFDLWIEAAEESYADVA
LSEQFRKVYGGFANAHMRLRAALQEEVEQLSERFGMPTRSEMDAAHRRIAELERLVRRMLRNAASPASKPAAAAQPAPVT
PAAGKRASSAGASAVARPAGAERAVKQGASKKAPVDKTATKKAAPKKAPAVSKPTKTTSPKKHGGATSSPELAARKPVAA
KKRGAQA

Sequences:

>Translated_407_residues
MASSGSDNGNFDDKARQYWAAWGDAMRHGQAGAAAQQPPPTSGSDAPQDWRKAVDWWSQLLPTQAAPQAQEAIDRFRTQA
GDWFGTMQQVAAQFAGRDTSANEVADAWRQAVQGQGEQLMQWTLGSLRGGSPGGFDPWLQDAAQALQKWREENAPWLDMP
AFGLNRNHQSRLQKLARAQQEFQAQSEAYGEQLKAAIEQAFARFASRLSEHESSGSQLTSARALFDLWIEAAEESYADVA
LSEQFRKVYGGFANAHMRLRAALQEEVEQLSERFGMPTRSEMDAAHRRIAELERLVRRMLRNAASPASKPAAAAQPAPVT
PAAGKRASSAGASAVARPAGAERAVKQGASKKAPVDKTATKKAAPKKAPAVSKPTKTTSPKKHGGATSSPELAARKPVAA
KKRGAQA
>Mature_406_residues
ASSGSDNGNFDDKARQYWAAWGDAMRHGQAGAAAQQPPPTSGSDAPQDWRKAVDWWSQLLPTQAAPQAQEAIDRFRTQAG
DWFGTMQQVAAQFAGRDTSANEVADAWRQAVQGQGEQLMQWTLGSLRGGSPGGFDPWLQDAAQALQKWREENAPWLDMPA
FGLNRNHQSRLQKLARAQQEFQAQSEAYGEQLKAAIEQAFARFASRLSEHESSGSQLTSARALFDLWIEAAEESYADVAL
SEQFRKVYGGFANAHMRLRAALQEEVEQLSERFGMPTRSEMDAAHRRIAELERLVRRMLRNAASPASKPAAAAQPAPVTP
AAGKRASSAGASAVARPAGAERAVKQGASKKAPVDKTATKKAAPKKAPAVSKPTKTTSPKKHGGATSSPELAARKPVAAK
KRGAQA

Specific function: Is required for expression of active PHA synthase. May play a role in the polymerization step [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: To the corresponding ORF in T.violacea [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010123 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 43894; Mature: 43763

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHCCCCC
>Mature Secondary Structure 
ASSGSDNGNFDDKARQYWAAWGDAMRHGQAGAAAQQPPPTSGSDAPQDWRKAVDWWSQL
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LPTQAAPQAQEAIDRFRTQAGDWFGTMQQVAAQFAGRDTSANEVADAWRQAVQGQGEQLM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHH
QWTLGSLRGGSPGGFDPWLQDAAQALQKWREENAPWLDMPAFGLNRNHQSRLQKLARAQQ
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHH
EFQAQSEAYGEQLKAAIEQAFARFASRLSEHESSGSQLTSARALFDLWIEAAEESYADVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LSEQFRKVYGGFANAHMRLRAALQEEVEQLSERFGMPTRSEMDAAHRRIAELERLVRRML
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RNAASPASKPAAAAQPAPVTPAAGKRASSAGASAVARPAGAERAVKQGASKKAPVDKTAT
HHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHCCCCHHHHHHCCCCCCCCCCHHHH
KKAAPKKAPAVSKPTKTTSPKKHGGATSSPELAARKPVAAKKRGAQA
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1396692 [H]