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The map label for this gene is rpoN [H]

Identifier: 21242713

GI number: 21242713

Start: 2300466

End: 2301869

Strand: Reverse

Name: rpoN [H]

Synonym: XAC1969

Alternate gene names: 21242713

Gene position: 2301869-2300466 (Counterclockwise)

Preceding gene: 21242714

Following gene: 21242712

Centisome position: 44.48

GC content: 67.45

Gene sequence:

>1404_bases
ATGAAGACGACCATTTCCGCCCAGCTTGGCCAGCAACTGCACCTCACCCCGCAGTTGCTGCAGTCGATCCGTCTGTTGCA
GTTGGACGGCATGCAGTTGGAACTGGAAATCCGTCGTGCCCTGGAAACCAATCCGTTGCTGGAGTTGGAAGAGCTGGCCG
ATAACGGGGACGAGACCGCCACCAACAACGATGGCGCCAGCGATATCGCCGCGTTCGACGAGCTGCCGGAAAGCACGATG
TGGGACGTGCAGGGCAGCAGTTGGAACGATGGGGACGACGATCGCATGCAGCGCGTGGCGGCCGGCGAGTCGACCGACCC
GCATGTGCGCATCCTGCGCGAGCTGGCGCTGGACCTGGACGAGGCGGCGTTGGGGGCCGCTGCGTTCTGGCTGGAGCAGA
CCGACGACGCCGGTTATCTGACCGAAGCGCTGTCGACGCTGCAACAGCTGGGCGCCACCCGTCTGGGCATGACCGCGCAG
GCGGTGGAAGCGATCCGGCAGCGGTTGCTGCACGGCGACCCGGCCGGTCTGGCCGCCTGCGATCTGCGCGAGTGTCTGCA
GGCCCAGTTGAGCGCCCTGCCCGGCCGCGTGCCGGCACGCCATCTGGCTGCGCGCATCCTCGACGGCGATCTGGAGCTGC
TGGCCGCGCACGACTACACGGTACTGGCGCGCGCGCACGATGCCGAAGTGGACGATGCGCGCGAAGCGGTGCGGCTGATC
CTGTCGCTGCAACCGCGCCCGGGCGACGACCTGCTGCAGGAAAGCAATGCCAGCGTGATCCCGGATGTGCTCGCCTGGCA
TGCCGACGACAGCTGGCGCGTCGCACTCAACCCGGCCACCACCCATCGCGTCAGCATCAACCCGGTGCACGAACGCGCGC
TGGCCGACGCCGGCGAGGCCGCGCAGCCGTTGCGCGACATGCTGCAGGAAGCGCGCTGGCTGACCCGCGGGCTGTCGATG
CGCTATGAGACCCTGCTGCGCGCTACGCGCGCCATCGTCGAACGCCAGGCCGCCTTCCTGGCGCGTGGCGAAGAAGCGAT
GGCACCGCTGACGCTGAAGGAAATCGCCGACGAGATCGGCATGCACGAATCGACTATTTCGCGCATCACCACCGGCAAGT
ACATCCAGACCCCACGCGGCACCTTCGAGCTCAAGCACTTCTTCGCCGTGCGGCTGGAAGGCGCCAGCGTCTCCGGCCAG
GCCGTCAAGGCGATGGTGCGCCGCTTGATCGAAAGCGAGCCGGCCGGCCGGCCGCTGGCCGACGAGGCGATTGCCGGATT
GCTGTCGCGGCAAGGCGTTAACATCGCGCGCCGCACCGTGGCCAAATATCGCGAACAACTTGATATCGCACCGGCACGCG
AGCGCCGCCGCACCACTAAACCGCTGCTTGCCCGGGCAGGATAA

Upstream 100 bases:

>100_bases
ACGAGTCACCTCACCCGACTCCCTGATTAGAAACCCGTCGCGTTGTCCTGCAAGATGCATTCCATCGCTGCTGTGTGCAG
CGCCCATCGGAAGCGCTTCC

Downstream 100 bases:

>100_bases
GGACTACTATGAGCAAACTCACCGTGCTGCTGGTCGATGACCACGAGGGCTTCATCAACGCTGCTATGCGTCATTTTCGC
AAGGTCGACTGGCTCAATAT

Product: RNA polymerase sigma-54 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MKTTISAQLGQQLHLTPQLLQSIRLLQLDGMQLELEIRRALETNPLLELEELADNGDETATNNDGASDIAAFDELPESTM
WDVQGSSWNDGDDDRMQRVAAGESTDPHVRILRELALDLDEAALGAAAFWLEQTDDAGYLTEALSTLQQLGATRLGMTAQ
AVEAIRQRLLHGDPAGLAACDLRECLQAQLSALPGRVPARHLAARILDGDLELLAAHDYTVLARAHDAEVDDAREAVRLI
LSLQPRPGDDLLQESNASVIPDVLAWHADDSWRVALNPATTHRVSINPVHERALADAGEAAQPLRDMLQEARWLTRGLSM
RYETLLRATRAIVERQAAFLARGEEAMAPLTLKEIADEIGMHESTISRITTGKYIQTPRGTFELKHFFAVRLEGASVSGQ
AVKAMVRRLIESEPAGRPLADEAIAGLLSRQGVNIARRTVAKYREQLDIAPARERRRTTKPLLARAG

Sequences:

>Translated_467_residues
MKTTISAQLGQQLHLTPQLLQSIRLLQLDGMQLELEIRRALETNPLLELEELADNGDETATNNDGASDIAAFDELPESTM
WDVQGSSWNDGDDDRMQRVAAGESTDPHVRILRELALDLDEAALGAAAFWLEQTDDAGYLTEALSTLQQLGATRLGMTAQ
AVEAIRQRLLHGDPAGLAACDLRECLQAQLSALPGRVPARHLAARILDGDLELLAAHDYTVLARAHDAEVDDAREAVRLI
LSLQPRPGDDLLQESNASVIPDVLAWHADDSWRVALNPATTHRVSINPVHERALADAGEAAQPLRDMLQEARWLTRGLSM
RYETLLRATRAIVERQAAFLARGEEAMAPLTLKEIADEIGMHESTISRITTGKYIQTPRGTFELKHFFAVRLEGASVSGQ
AVKAMVRRLIESEPAGRPLADEAIAGLLSRQGVNIARRTVAKYREQLDIAPARERRRTTKPLLARAG
>Mature_467_residues
MKTTISAQLGQQLHLTPQLLQSIRLLQLDGMQLELEIRRALETNPLLELEELADNGDETATNNDGASDIAAFDELPESTM
WDVQGSSWNDGDDDRMQRVAAGESTDPHVRILRELALDLDEAALGAAAFWLEQTDDAGYLTEALSTLQQLGATRLGMTAQ
AVEAIRQRLLHGDPAGLAACDLRECLQAQLSALPGRVPARHLAARILDGDLELLAAHDYTVLARAHDAEVDDAREAVRLI
LSLQPRPGDDLLQESNASVIPDVLAWHADDSWRVALNPATTHRVSINPVHERALADAGEAAQPLRDMLQEARWLTRGLSM
RYETLLRATRAIVERQAAFLARGEEAMAPLTLKEIADEIGMHESTISRITTGKYIQTPRGTFELKHFFAVRLEGASVSGQ
AVKAMVRRLIESEPAGRPLADEAIAGLLSRQGVNIARRTVAKYREQLDIAPARERRRTTKPLLARAG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=482, Percent_Identity=39.4190871369295, Blast_Score=296, Evalue=2e-81,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 51273; Mature: 51273

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTISAQLGQQLHLTPQLLQSIRLLQLDGMQLELEIRRALETNPLLELEELADNGDETA
CCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCHHHHHHHCCCCCCC
TNNDGASDIAAFDELPESTMWDVQGSSWNDGDDDRMQRVAAGESTDPHVRILRELALDLD
CCCCCHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCHH
EAALGAAAFWLEQTDDAGYLTEALSTLQQLGATRLGMTAQAVEAIRQRLLHGDPAGLAAC
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHCCCCCCHHHH
DLRECLQAQLSALPGRVPARHLAARILDGDLELLAAHDYTVLARAHDAEVDDAREAVRLI
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHEEEECCEEEEEECCCCCCHHHHHHHHHH
LSLQPRPGDDLLQESNASVIPDVLAWHADDSWRVALNPATTHRVSINPVHERALADAGEA
HHCCCCCCHHHHHCCCCCCCCHHHHCCCCCCCEEEECCCCCEEEECCHHHHHHHHCCCHH
AQPLRDMLQEARWLTRGLSMRYETLLRATRAIVERQAAFLARGEEAMAPLTLKEIADEIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHC
MHESTISRITTGKYIQTPRGTFELKHFFAVRLEGASVSGQAVKAMVRRLIESEPAGRPLA
CCHHHHHHHCCCCEEECCCCCHHHEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCH
DEAIAGLLSRQGVNIARRTVAKYREQLDIAPARERRRTTKPLLARAG
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHCHHHHHHCCC
>Mature Secondary Structure
MKTTISAQLGQQLHLTPQLLQSIRLLQLDGMQLELEIRRALETNPLLELEELADNGDETA
CCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCHHHHHHHCCCCCCC
TNNDGASDIAAFDELPESTMWDVQGSSWNDGDDDRMQRVAAGESTDPHVRILRELALDLD
CCCCCHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCHH
EAALGAAAFWLEQTDDAGYLTEALSTLQQLGATRLGMTAQAVEAIRQRLLHGDPAGLAAC
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHCCCCCCHHHH
DLRECLQAQLSALPGRVPARHLAARILDGDLELLAAHDYTVLARAHDAEVDDAREAVRLI
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHEEEECCEEEEEECCCCCCHHHHHHHHHH
LSLQPRPGDDLLQESNASVIPDVLAWHADDSWRVALNPATTHRVSINPVHERALADAGEA
HHCCCCCCHHHHHCCCCCCCCHHHHCCCCCCCEEEECCCCCEEEECCHHHHHHHHCCCHH
AQPLRDMLQEARWLTRGLSMRYETLLRATRAIVERQAAFLARGEEAMAPLTLKEIADEIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHC
MHESTISRITTGKYIQTPRGTFELKHFFAVRLEGASVSGQAVKAMVRRLIESEPAGRPLA
CCHHHHHHHCCCCEEECCCCCHHHEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCH
DEAIAGLLSRQGVNIARRTVAKYREQLDIAPARERRRTTKPLLARAG
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113171; 10984043 [H]