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The map label for this gene is fliA [H]

Identifier: 21242677

GI number: 21242677

Start: 2258166

End: 2258933

Strand: Reverse

Name: fliA [H]

Synonym: XAC1933

Alternate gene names: 21242677

Gene position: 2258933-2258166 (Counterclockwise)

Preceding gene: 21242678

Following gene: 21242676

Centisome position: 43.65

GC content: 66.67

Gene sequence:

>768_bases
ATGAGCACCGCTACCGCAACCACGGCCAGCGCCCAGTACCGCGCCGTGCAGCGCAACAACGCCAACGACGTGGTGACCCA
GCACGCCGACCTGGTCCGCCGCATCGCCCACCATCTGGCAGCGCGTCTGCCGGCCAGCGTGGAAGTGGACGACCTGATCC
AGGCCGGCATGATCGGCCTGATCGAAGCCTCGCGCAGCTACGACTCCGAACAGGGCGCGTCGTTCGAAACCTATGCCTCG
ATCCGCATCCGCGGCTCGATGATCGACGAGATTCGCAGGGGCGACTGGGTGCCGCGTTCGGTGCACCGCCGCGCCCGCGA
TGCCGCCGCCGCCGTGCGCAAGATCGAACAGAACACCGGCCGCGCCGCCGCCGCCACCGAAGTGGCCGCCGCCATGGAAA
TGCCGCTGCCCGATTACCTGCGCCTGATGGAAGATGCGGCACGTGGCCAGGTGCTGAGCCTGGAATCGCGCATCGAAGAC
CACGGCGAGCTGGACACCACCGCCAAGGGCGGCCCCAATCCGCAGCAGATGATGGAGCGTGGCGAATTCGGCCGCGAGCT
GGGCAAGGCCATCGGCCAACTGCCCGAGCGCGAGCAACTGGTGCTGTCGCTGTACTACGAACAGGAATTGAACCTGAAGG
AAATCGGCGCCGTGCTGGGCGTCAGCGAATCGCGCGTGTGCCAGATCCACGGCCAGGCGGTGGTGCGTTTGCGTGGCCGC
CTCAAAGTCTTCGAACTGGCCGATGCCGGTGTCGAAATTGACGACTAA

Upstream 100 bases:

>100_bases
CGGAAATCGCACGCCGTACCTCGCGCTGGCAGGCCCCGACCGTGCCGCGCGGCAACGTCGAGTTCTTTGTCGAACGCATC
ATCCAACGGGGAGTGGCCGC

Downstream 100 bases:

>100_bases
TTTTTTGTGTTAGGAGCTTGATGTGAACAAGAACATGCGGATCCTGATCGTGGACGACTTCTCGACGATGCGACGTATCG
TCAAGAATCTGTTGGGCGAT

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSTATATTASAQYRAVQRNNANDVVTQHADLVRRIAHHLAARLPASVEVDDLIQAGMIGLIEASRSYDSEQGASFETYAS
IRIRGSMIDEIRRGDWVPRSVHRRARDAAAAVRKIEQNTGRAAAATEVAAAMEMPLPDYLRLMEDAARGQVLSLESRIED
HGELDTTAKGGPNPQQMMERGEFGRELGKAIGQLPEREQLVLSLYYEQELNLKEIGAVLGVSESRVCQIHGQAVVRLRGR
LKVFELADAGVEIDD

Sequences:

>Translated_255_residues
MSTATATTASAQYRAVQRNNANDVVTQHADLVRRIAHHLAARLPASVEVDDLIQAGMIGLIEASRSYDSEQGASFETYAS
IRIRGSMIDEIRRGDWVPRSVHRRARDAAAAVRKIEQNTGRAAAATEVAAAMEMPLPDYLRLMEDAARGQVLSLESRIED
HGELDTTAKGGPNPQQMMERGEFGRELGKAIGQLPEREQLVLSLYYEQELNLKEIGAVLGVSESRVCQIHGQAVVRLRGR
LKVFELADAGVEIDD
>Mature_254_residues
STATATTASAQYRAVQRNNANDVVTQHADLVRRIAHHLAARLPASVEVDDLIQAGMIGLIEASRSYDSEQGASFETYASI
RIRGSMIDEIRRGDWVPRSVHRRARDAAAAVRKIEQNTGRAAAATEVAAAMEMPLPDYLRLMEDAARGQVLSLESRIEDH
GELDTTAKGGPNPQQMMERGEFGRELGKAIGQLPEREQLVLSLYYEQELNLKEIGAVLGVSESRVCQIHGQAVVRLRGRL
KVFELADAGVEIDD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=217, Percent_Identity=50.6912442396313, Blast_Score=202, Evalue=2e-53,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 28003; Mature: 27872

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTATATTASAQYRAVQRNNANDVVTQHADLVRRIAHHLAARLPASVEVDDLIQAGMIGL
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IEASRSYDSEQGASFETYASIRIRGSMIDEIRRGDWVPRSVHRRARDAAAAVRKIEQNTG
HHHHHCCCCCCCCCEEHEEEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
RAAAATEVAAAMEMPLPDYLRLMEDAARGQVLSLESRIEDHGELDTTAKGGPNPQQMMER
CHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEHHHHHHHCCCCCCCCCCCCCHHHHHHH
GEFGRELGKAIGQLPEREQLVLSLYYEQELNLKEIGAVLGVSESRVCQIHGQAVVRLRGR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEECHHHHHHHHCC
LKVFELADAGVEIDD
EEEEEECCCCCCCCC
>Mature Secondary Structure 
STATATTASAQYRAVQRNNANDVVTQHADLVRRIAHHLAARLPASVEVDDLIQAGMIGL
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IEASRSYDSEQGASFETYASIRIRGSMIDEIRRGDWVPRSVHRRARDAAAAVRKIEQNTG
HHHHHCCCCCCCCCEEHEEEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
RAAAATEVAAAMEMPLPDYLRLMEDAARGQVLSLESRIEDHGELDTTAKGGPNPQQMMER
CHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEHHHHHHHCCCCCCCCCCCCCHHHHHHH
GEFGRELGKAIGQLPEREQLVLSLYYEQELNLKEIGAVLGVSESRVCQIHGQAVVRLRGR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEECHHHHHHHHCC
LKVFELADAGVEIDD
EEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]