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The map label for this gene is greA

Identifier: 21242607

GI number: 21242607

Start: 2160878

End: 2161354

Strand: Direct

Name: greA

Synonym: XAC1863

Alternate gene names: 21242607

Gene position: 2160878-2161354 (Clockwise)

Preceding gene: 161485715

Following gene: 21242608

Centisome position: 41.75

GC content: 61.64

Gene sequence:

>477_bases
ATGAGAGCACCCATGACATCGAAGGGCGCGCAGCGCCTGCGCGAAGAGCTGGAACACCTGAAGTCGGTCAAGCGCCCGGA
GGTGATCAATGCGATCGCCGAAGCGCGTGCGCATGGCGATCTGAAGGAAAACGCCGAATACCATGCGGCGCGCGAGCAAC
AGAGCTTTATCGAAGGCCGCATCAAGCAGCTGGAAAGCGAGCTGTCGCATGCCGAAATCATCGACATCACCAAGTTGTCG
GCCGGCACCAAGATCGTGTTCGGCGCCACGGTGACGCTGGCCGATACCGAGACCGACGAAGAGAAGCGCTACCAGATCGT
TGGCGATCTGGAAGCGGACATCAAGATGGGCCTGATCGCGATCTCCTCGCCGTTGGCGCGTGCGCTGATCGGCAAGCTGG
AAGGCGATTCGGTCACCATCGATGCGCCGGCCGGCCAGCGCGAGTACGAAGTCGTCAGCGTCGCCTACCTGGACTGA

Upstream 100 bases:

>100_bases
CGACCACTGTCGCCGGCGCCAAGGCGCTGGTGCAATCGCTGGAATTCCGCGGCACCGGCCCCGTGTGGTCGCTGCAGGAA
CTGCACAAGGAGCTGGAAGC

Downstream 100 bases:

>100_bases
CCGGCATCACTGCGCGATGACCACCGCCACATTGCTGCTGCCGGAAAAACGCCGCCTGCCCGGAACCCTGGGCGATACGG
CAGTGGCGCGTGCGCTTGCG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MRAPMTSKGAQRLREELEHLKSVKRPEVINAIAEARAHGDLKENAEYHAAREQQSFIEGRIKQLESELSHAEIIDITKLS
AGTKIVFGATVTLADTETDEEKRYQIVGDLEADIKMGLIAISSPLARALIGKLEGDSVTIDAPAGQREYEVVSVAYLD

Sequences:

>Translated_158_residues
MRAPMTSKGAQRLREELEHLKSVKRPEVINAIAEARAHGDLKENAEYHAAREQQSFIEGRIKQLESELSHAEIIDITKLS
AGTKIVFGATVTLADTETDEEKRYQIVGDLEADIKMGLIAISSPLARALIGKLEGDSVTIDAPAGQREYEVVSVAYLD
>Mature_158_residues
MRAPMTSKGAQRLREELEHLKSVKRPEVINAIAEARAHGDLKENAEYHAAREQQSFIEGRIKQLESELSHAEIIDITKLS
AGTKIVFGATVTLADTETDEEKRYQIVGDLEADIKMGLIAISSPLARALIGKLEGDSVTIDAPAGQREYEVVSVAYLD

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=154, Percent_Identity=61.6883116883117, Blast_Score=204, Evalue=2e-54,
Organism=Escherichia coli, GI87082261, Length=160, Percent_Identity=33.75, Blast_Score=89, Evalue=1e-19,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_XANAC (Q8PLD9)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642189.1
- ProteinModelPortal:   Q8PLD9
- SMR:   Q8PLD9
- GeneID:   1155934
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1863
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   XAXO190486:XAC1863-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17358; Mature: 17358

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAPMTSKGAQRLREELEHLKSVKRPEVINAIAEARAHGDLKENAEYHAAREQQSFIEGR
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHH
IKQLESELSHAEIIDITKLSAGTKIVFGATVTLADTETDEEKRYQIVGDLEADIKMGLIA
HHHHHHHHCHHHHHEEEECCCCCEEEEEEEEEEECCCCCCHHHEEEECCCCCCHHHHHHH
ISSPLARALIGKLEGDSVTIDAPAGQREYEVVSVAYLD
HHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEECC
>Mature Secondary Structure
MRAPMTSKGAQRLREELEHLKSVKRPEVINAIAEARAHGDLKENAEYHAAREQQSFIEGR
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHH
IKQLESELSHAEIIDITKLSAGTKIVFGATVTLADTETDEEKRYQIVGDLEADIKMGLIA
HHHHHHHHCHHHHHEEEECCCCCEEEEEEEEEEECCCCCCHHHEEEECCCCCCHHHHHHH
ISSPLARALIGKLEGDSVTIDAPAGQREYEVVSVAYLD
HHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217